Herbal medicine , also called botanical medicine or phytomedicine,refers to the use of any plant seeds, berries, roots

, leaves, bark or flowers for medicinal purposes. There are many proceses patented for commercial extraction of plant ingredients. The general techniques of extraction of medicinal plants include maceration,percolation & hot continous extraction (soxhlet). Maceration is the simple method by soaking the powder material in the solvent for breaking the cell wall and foll.owed by destilation proces 1. Extraction Ethanol Absolute
Fresh Grass Mincing

Shadow dried/ oven 60 C

Grinding (Powder)

1kg of powdered plant Soaked in 10 L water 600C, 20 min

Filter to obtain crude yellow aquaeous extract

Add 100% Ethanol absolute 1:4

Filter the supernatant solution and gel pellet was discharge

yellow extract destilated 600C to get concentrated extract

2. Extraction Ethanol 96% (technical grade) Fresh Grass Mincing Shadow dried/ oven 60 C Grinding (Powder) 1kg of powdered plant Soaked in 10 L water 600C. 20 min Filter to obtain crude yellow aquaeous extract Add 96% Ethanol technical grade 1:4 Filter the supernatant solution and gel pellet was discharge yellow extract destilated 600C to get concentrated extract .

Extraction Water 8-10 times Fresh Grass Mincing Shadow dried/ oven 60 C Grinding (Powder) 1kg of powdered plant Soaked in 10 L water 600C. 20 min Filter to obtain crude yellow aquaeous extract 8-10X Add powdered plant 1/10 (w/v) from yellow extract Filter the crude yellow aquaeous extract Coated to the feed .3.

4. Methanol extract Fresh Grass Mincing Shadow dried/ oven 60 C Grinding (Powder) 550g of powdered plant Soaked in 3 L methanol for 7 day Filtered through cotton plug Followed by whatman filter paper no 1 Extract destilated 600C To get concentrated extract .

5. Directly mixing to the feed Fresh Grass Mincing Shadow dried/ oven 60 C Grinding (Powder) Mix to the feed 15 -20 % .

Uji kandungan kimia ekstrak 1.Cemaran logam berat (heavy metal) 8.EXTRACT PARAMETER Menurut buku Monografi Ekstrak dan Parameter Standar Ekstrak (Badan POM).Uji cemaran aflatoksin (aflatoxin) C.MPN Coliform c.Kadar total golongan kandungan kimia (Total chemical content) 3.Organoleptik ekstrak (organolepthic extract) 3. Cemaran Mikroba : (microbacterial contamination) a.Bobot jenis (specific gravity) 3.Kadar kandungan kimia tertentu (specific chemical content) MACHINE FOR GRXTRACTION .Sisa pelarut (solvent residue) 6. Parameter non spesifik 1.Uji Angka kapang dan khamir (mold) d.Kadar abu (ash) 5.Kadar air (moisture) 4. parameter yang harus diujikan terhadap ekstrak sedikitnya antara lain meliputi : A.ALTB b. Parameter spesifik 1.Pola kromatogram (chromaogaphi characteristic) 2.Susut pengeringan 2.Residu pestisida (pesticide residue) 7.Identitas ekstrak ( extract identity) 2.Senyawa terlarut dalam pelarut tertentu (soluble compound & solvent) B.

Grass Cutter OVEN Hammer Mill Mixer + heater .

Filter Press Rotary Evaporator 50 l/h Alcohol/Ethanol destilator .

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