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FERNANDO J. FERRER Cell: (858) 382-9759 ff8ea960@westpost.net San Diego CA 92128 www.linkedin.

com/in/fernandoferrer2010 SYNTHETIC CHEMIST More than 10 years of academic and pharmaceutical experience designing, synthesi zing, scaling up and purifying peptide sequences up to 90 residues. Hands-on ex perience in labeling and conjugating long peptides to cancer drugs (including do xorubicin and cilastatin). Mastery of different Fmoc peptide chemistries for sy nthesis of difficult peptide sequences. Introduced new technologies (microwave synthesis) and developed methods and protocols to improve peptide quality and pr oduction. Developed HPLC methods in instruments from Agilent, Waters and Gilson . Experience in supervising and training research associates. Areas of experti se include: Peptide design and drug modification Microwave peptide synthesis Troubleshooting synthesizers and HPLC Solid and solution phase synthesis Peptide drug conjugation Peptide fluorescence labeling Heterocyclic synthesis Design of new peptide coupling reagents (HATU analogs) Designing protocols for peptide synthesizers Flash chromatography, HPLC (reverse phase), HIC and SEC TLC (analytical and preparative), H-NMR and FT-IR Scale-up synthesis Organometallic synthesis Gel electrophoresis (SDS-PAGE) PROFESSIONAL EXPERIENCE AMYLIN PHARMACEUTICALS INC, San Diego, CA 2005 2010 Investigator 2008 2010 Drug design (SAR) and scale-up of drug candidates. Developed a novel protocol to scale-up the synthesis of drug candidates, obtain ing two grams of pure material using the APEX peptide synthesizer. Developed a simple solution phase peptide method saving $1K per gram synthesize d using an EDC and HOAt coupling system. Designed new peptide analogs (SAR) with improved potency in food intake assay. Successfully automated a manual coupling system (DIC/HOAt in DMF/DCM) in two di fferent peptide synthesizers (Liberty and APEX), increasing the purity of diffic ult sequences and lowering racemization. Solved the problem of obtaining peptides with a Cysteine at the carboxylic term inus, allowing the synthesis of long GLP-1 analog peptides in good yield. Staff Scientist 2005 2008 Peptide synthesis and method development. Introduced Microwave Peptide Synthesis (MPS), increasing the amount of difficul t peptides synthesized by 50%. Automated and developed protocols in the Liberty (microwave synthesizer) and AP EX to incorporate pseudoproline dipeptides, acid fluorides and pentafluorophenyl esters, increasing the success of difficult peptides synthesized by 30%. Automated the incorporation of Hmb amino acids in the peptide sequence to avoid aspartamide formation as a major impurity. Validated methods for peptide synthesis to obtain 80 peptides per year.

Developed a solid phase segment condensation to obtain small peptides in one st ep. FERNANDO J. FERRER ff8ea960@westpost.net Page Two SANFORD BURNHAM MEDICAL RESEARCH INSTITUTE, La Jolla, CA 2000 2005 Peptide Chemist In charge of a peptide core facility. Successfully applied various synthetic methods to obtain doxorubicin, cin and cilastatin peptide conjugates for drug delivery systems. Developed a chemical free method to obtain sulfur bridged peptides in eld. Implemented several synthetic methods to obtain pure labeled peptides yield. Successfully developed automated protocols to incorporate fluorescein mine into peptide sequences to be detected by electron microscopy.

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UNIVERSITY OF MASSACHUSETTS, Amherst, MA 1992 2000 Postdoctoral Associate 1999 2000 Research in peptide chemistry. Developed protocols for using Bsmoc-protected amino acids in the 433A (ABI) ins trument, reducing the deblocking time to one minute. Graduate Student 1992 1999 Research in designing new coupling peptide reagents. Developed novel coupling reagents for peptide synthesis with a better yield and optical purity than those obtained using HATU and HBTU. EDUCATION Ph.D., Organic Chemistry UNIVERSITY OF MASSACHUSETTS, Amherst, MA (Prof. L.A. Carpino, Advisor) M.S., Organic Chemistry UNIVERSIDAD SIMON BOLIVAR, Caracas, Venezuela B.S., Chemistry Teacher UNIVERSIDAD PEDAGOGICA LIBERTADOR, Caracas, Venezuela FERNANDO J. FERRER ff8ea960@westpost.net Page Three PUBLICATIONS New synthesis of alpha-bromo-alpha,beta-unsaturated esters. Zapata, A.J.; Ferre r, F.J. Synth. Commun. 1986, 16 (13), 1611. Effect of N-chloro structure and 1-substituent on sigma-substitution (additionelimination) in pyrroles. De Rosa, M.; Cabrera, G.; Ferrer, F.J. J. Org. Chem. 1989, 54 (22), 5347. Carbonyl olefination with *-stannyl ester enolates: A new synthesis of alpha,be ta-unsaturated esters. Zapata, A.J.; Nuez B.M.; Ferrer, F.J. J. Organomet. Chem. 1992, 424 (3), C9. Synthesis of the two isomeric benzo derivatives of 1-hydroxy-7-azabenzotriazole and their effectiveness as peptide coupling reagents. Ferrer, F. J.; Carpino, L .A. Proceedings of the 2nd International, 17th American Peptide Symposium, 2001, San Diego, CA. Synthesis of 1-hydroxy-7-azabenzotriazole-N-oxide, 1-hydroxy 1- and 2-azanaphth otriazoles and their various HATU and HBTU derivatives. Effectiveness as couplin

g reagents. Ph.D. Thesis, University of Massachusetts, Amherst, 1999. The 5,6-and 4,5 benzo derivatives of 1-hydroxy-7-azabenzotriazole. Louis A. Car pino and Fernando J. Ferrer. Org. Lett. 2001, 3, 2793-2795. The uronium/guanidinium peptide coupling reagents: finally the true uronium sal ts. Louis A. Carpino, Hideko Imazumi, Ayman El-Faham, Fernando J. Ferrer, Chongw u Zhang, Yunsub Lee, Bruce M. Foxman, Peter Henklein, Chistine Hanay, Clemens Mu gge, Holger Wenschunh, Jana Klose, Michael Beyermann and Michael Bienert. Angew. Chem. 2002 (41), 441. The uronium/guanidinium peptide coupling reagents: finally the true uronium sal ts. Carpino, L.A.; Imazumi, H.; El-Faham, A.; Ferrer, F.J.; Zhang, C.; Lee, Y.; Foxman, B.M. Henklein, P.; Hanay, C.; Mugge, C. Wenschuh, H.; Klose, J.; Beyerma nn, M.; Bienet, M. Biopolymers. 71, 349. 2003. Use of Hmb-back-bone protected amino acids in microwave peptide synthesis. (In preparation). Fernando J. Ferrer. PRESENTATIONS AT SCIENTIFIC CONFERENCES Synthesis of the two isomeric benzo derivatives of 1-hydroxy-7-azabenzotriazole and their effectiveness as peptide coupling reagents. Ferrer, F. J.; Carpino, L .A. Proceedings of 2nd International, 17th American Peptide Symposium, 2001, San Diego, CA. Microwave-assisted synthesis of human nesfatin: an efficient tool for synthesis of long and challenging peptide sequences. ACS, 2008. Philadelphia, PA.