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1626 Norristown Rd.

, Ambler, PA 19002
610-368-1120 (Mobile), 215-382-7705 x 2105 (O) * ying_homan@hotmail.com
Ying Homan
Summary
An experienced scientist with about eight years of biomedical research experienc
e in both academic and industrial settings. Skilled in bioassays using qPCR, FAC
S, ELISA, Western blot analysis and fluorescence cell staining; experienced with
primary cell culture and establishing immortalized cell lines from primary cell
s; expertise with compound screening using immortal cell lines and primary cell
lines. Knowledge of establishing in vivo and in vitro models for stem cell resea
rch. Expertise in molecular mechanistic studies of cellular functions in stem c
ell regeneration research.
A quick learner capable of multitasking; with extensive knowledge and skills in
modern molecular biology and cell biology; detail orientated with good organizat
ional skills; experienced in data organization and statistical analysis using Ex
cel, Statgraphics plus 5.1; experienced with pathway finding database search, su
ch as, Ingenuigy; experienced in result presentation using PowerPoint.
Professional Experience
February 2009 - present
Aderans Research Institute, Inc.
Philadelphia, PA
Senior Scientist I
* Designed and conducted biological assays to study mechanism underlying our cel
l products potencies. Screened agonists and antagonists that impacts biological
functions of the cells using GFP reporting constructs
Techniques employed include qPCR, immunofluorescence cell staining, FACS, Wester
n blot, FISH , molecular cloning to generate the reporter constructs, lipofectio
n and magnetofection, immunohistochemical staining and GFP detection by FACS ana
lysis.
* Designed and conducted bioassays to identify and validate biomarkers for regen
erative activity of human primary cells. We explored both gene array approach a
nd SILAC protein analysis approach. We also screened cell surface markers for m
arker identification.
Techniques employed include: SILAC media culture, western blotting and immunoflu
orescence staining; mRNA array analysis and qRT-PCR analysis, GUAVA, FACS and ma
gnetic cell sorting.
* Established immortalized human dermal cell lines from primary culture of human
scalp using lentiviruses to study the molecular basis of human dermal cell rege
nerative activity.
February 2008 - February 2009
Aderans Research Institute, Inc.
Philadelphia, PA
Scientist IV
* Established semi high throughput protocols to screen lineage markers of cell p
roducts for phase II clinical trial.
* Studied and screened for protein cell surface markers for lineage identificati
on of our cell products for future clinical trial and production.
* Determined the effects of BMP2, BMP4, and Noggin on the mouse dorsal skins usi
ng immunohistochemistry and fluorescence in-situ hybridization
May 2005 January 2008
Aderans Research Institute, Inc.
Philadelphia, PA
Scientist I
* Conducting bioassays of mRNA and miRNA expression of clinical trial samples us
ing quantitative RT-PCR.
* Determined the effects of SHH, FGF2 and other growth factors on the potency of
cultured human primary cells from human clinical trials
* Optimized culture conditions for primary human cells, and wrote SOPs for trans
ferring culture techniques to the manufacturing department.
* Participated in the development of animal models using nu/nu and NOD/SCID mice
for testing skin cell regenerative activities in vivo.

October 2004 April 2005


GlaxoSmithKline
Upper Providence, PA
Associate Scientist (Contractor)
* Participated in the study to determine the effects of a protein kinase inhibit
or on the level of hemoglobin in BDF1 anemic mouse model.
* Participated in the study to determine the mechanism of this protein kinase in
hibitor by analyzing the gene expression profile of BDF1 anemic mouse bone marro
w under stress.
Responsibilities included: IP dosing the BDF1 mice (irradiated and paraplatin tr
eated) and cardiac puncture to obtain whole blood from mouse; blood sample proce
ssing using Advia 120; gene expression analysis using Agilent and TaqMan; wester
nblot and immunoprecipitation analysis to identify gene regulation and signal tr
ansduction mechanism; data analysis using Graphpad and Excel spreadsheet.
January 2003 December 2003
Medical Service, U.S. Human Health, Merck & Co., Inc.
North Wales, PA
Information Scientist (Contractor/Temp)
* Extensively searched and compiled medical information to support Merck medical
education projects for vaccine sale representative training, and to aid vaccine
business analysis and forecasting.
* Analyzed and distilled the information from extensive database search on post-
surgery infection rates, and presented to business teams to support of commercia
l assessment and other market analyses.
* Interpreted vaccine related questions from healthcare professionals and provid
ed information searching and letter editing to reply the questions from Health C
are professionals.
June 2000 December 2002
Dept of Pharmacology, University of Pennsylvania School of Medicine
Philadelphia, PA
Research Specialist: Molecular genetic studies of Nac-1 gene and its biological
functions
* Determined the genomic organization of mouse and human Nac-1 genes by extensiv
e mining of genetic databases, including sequence editing, exon/intron identifi
cation, deduced amino acid sequence analysis and homologous gene identification.
* Cloned mouse Nac-1 gene by screening mouse BAC library , identified the chromo
some in which Nac-1 is located, and determined the expression levels of Nac-1 ge
ne in cocaine-treated rats.
* Identified critical regulation factor of Nac-1 in transcriptional activity stu
dy using luciferase reporter assays.
* Other responsibilities included: maintaining and ordering lab instruments, ra
diation safety monitoring and swipe tests.
May 1998 June 2000
Coriell Institute for Medical Research
Camden, NJ
Research Scientist
* Participated in the study to compare the behaviors of normal and leukemia stem
cells in wild type and NOD/SCID mice.
* Developed protocols to successfully transducer Lin-primary cell lines using le
ntiviral vectors in combination with different cytokines.
* Optimized RNA isolation and RT-PCR gene transcription assay using very low num
bers of FACS sorted cells.
* Other responsibilities included: Irradiation and general management of NOD/SC
ID mouse colonies in a well controlled animal facility. Education
M.A., Biochemistry
University of Alaska Fairbank
Fairbank, Alaska
M.Sc., Microbiology
Beijing Agricultural University
Beijing, P.R. China
B.S., Biology
Hebei University
Hebei, P.R. China
Publications 1)Zheng, Y, Nace, A, Chen, W,Watkins, K, Sergott,L, Homan Y,Vandeb
erg,L, Breen,M, and K. Stenn, Mature Follicles generated from Dissociated cells:
A Universal Mechanism of Folliculoneogenesis.Dev Dyn2010, manuscript accepted.
2)Zheng,Y, Nace,A, Chen,W, Homan Y, Watkins,K, Sergott, L, Hsieh,J.C, VandeBerg,
J, Breen, M, Stenn, K. (2010) Common mechanism of hair follicle formation from
dissociated trichogenic and epidermal and dermal cells. Society for Investigativ
e Dermatology Conference. Georgia.
3)Mackler S, Pacchioni A, Degan R, Homan Y, Conti AC, Kalivas P and Blendy JA; (
2008) Requirement for the POZ/BTB protein NAC1 in acute but not chronic psychomo
tor stimulant response. Behav. Brain Res. Feb11; 187 (1) 48-55
4) Erickson-Miller, LC, Homan,Y, Chomo, M. Valoret, E, Elefante, L, Landis A, C
hadderton, T, and Hopson, C; (2007) The Characterization of DYRK3 mRNA and the
Utility of DYRK3 Inhibitors in a Carboplatin/Radiation Mouse Model of Anema Blo
od supplement for conference 110: 2202
5) Mackler SA, Homan YX, Korutla L, Conti AC, Blendy JA. (2003) The mouse nac1
gene, encoding a cocaine-regulated bric-a-brac tramtrac broad complex/pox virus
and zinc finger protein, is regulated by ap1. Neuroscience. 2003; 121(2):355-61.
6) Mackler SA, Homan YX, Korutla L, Conti AC, Blendy JA. (2002) The mouse Nac1
gene, encoding a cocaine-regulated POZ/BTB protein, is regulated byAP1. Society
for Neurosciences International Conference
7) D. Bonnet, Y. Xu and J. Luongo (1999) Heterogeneity at the Leukemic Stem Cel
l Level 1999 Annual Meeting of The American Society of Hematology
Patents
1) Zheng, Y, Boucher, M, Homan, Y, Hollow, C, Mamontov, P and Stenn, K. Methods
And Compositions For Increasing Trichogenic Potency of Dermal Cells Application
No. 61/227,540 and No. 61/187,894
2) Watkins, K, Chen,W, Homan,Y, Stenn, K, Zheng, Y, Nace, A. Trichogenic Dermal
Cells Patent Application in preparation
References
References are available on request.