Annual Report

2003-2004

Pakistan Atomic Energy Commission

PAEC Annual Report for the year 2003-2004, prepared as a statutory requirement under the provisions of section 15(b) of PAEC Ordinance 1965, aims to compile a concise description of Commission's activities undertaken and accomplished during this period.

While congratulating all the scientists, engineers, technicians, administration and financial personnel on their brilliant performance in their respective fields, the Commission places on record their valuable services with admiration and pride.

(Dr. KhwajaTaldram) Secretary Pakistan Atomic Energy Commission Islamabad

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Highlights Nuclear Power Physical Sciences & Engineering Bio-Sciences Nuclear Minerals Safety Human Resource Development Projects International Relations Finance Publications 1 5 11 23 41 46 49 51 53 55 57 .

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(ii) preparation ofthe feasibility report of the C-2 Project for the EXIM Bank of China. In addition. KANUPP was restarted on January 22. 2004 the plant operated at an average load of about 30 MWe.14% and 63. The total on line time of the Turbine-Generator was 5569 hours thus raising the on line time of Turbine-Generator to 22953 hours since connection to grid. Institute for Nuclear Power and Karachi Nuclear Power Complex entered into a Memorandum of Understanding (MOU) for joint technical activities. The theory has been applied to laser induced plasmas and the estimate of B-field turns out to be in agreement with the experimental observations.52 GWh. In this regard two major activities were: (i) preparation and submission of the final PC-1 ofthe project in April 2004. aimed at continued safe operation of KANUPP. meters for PARR-1. 5 at PARR-1. The cumulative gross generation since NPD was raised to 10. Reactor Experiments Experimentation has been started to measure the reactor kinetic para1 Annual Report 2003-2004 .831. INUP continued to provide requisite technical support to Chashma Nuclear Power Plant in In-Core fuel Management. Work for the development of nuclear reactor control system has been initiated including classical control. INUP continued work on analytical engineering studies related to the Pressurized Water Reactors (PWRs). 2004 to June 30. Neutron and X-Ray Diffraction The IAEA TC project PAK/4/044 has been successfully completed. PARR-2 was operated for 143 hours irradiating 1242 samples. developed and installed at beam tube No. 2002 after completion of the nominal design life of 30 years to fulfill regulatory requirements for relicensing. plant cost. Under this project. a small angle neutron scattering instrument has been designed. fuel cost and financial package forthe C-2 project were finalized. The project was approved by the ECNEC in July 2004. 560 capsules containing different samples were irradiated. conventional state feedback control. Gross electricity generated was 78. Operational Core analysis and Safety Analysis and participated in the second Refueling Outage (RFO) of CHASNUPP. After various rounds of negotiations with Chinese organizations. Plasma Physics A theory has been developed to describe the time evolution of nonequilibrium incompressible plasma to understand the mechanism of magnetic field generation taking into account the dynamics of both electrons and ions. The average plant capacity and availability factors were 59. During the period of January 22.40% respectively. Reactor Power Regulation Console at KANUPP CHASNUPP operated safely and generated a total of 1682 GWh of electrical energy from July 2003 to June 2004 of which 1558 GWh was exported to WAPDA. ASAG continued its contribution in planning of the C-2 project. NUCLEAR SCIENCE & TECHNOLOGY Reactor Operation During the period under report PARR-1 was operated for 550 hours including 23 continuous operations of 12 hours duration each at full power.HIGHLIGHTS NUCLEAR POWER KANUPP was shutdown on December 5. The final PC-1 was prepared based on these costs and financial terms. On completion of the regulatory requirement. PNRA issued a special license to KANUPP for restarting ofthe reactor.52% and 14.35 GWh with availability factor and gross capacity factor of 70. optimal control and state feedback assisted classical control. 2004 at 22:15 hrs with a new lease of life.49% respectively.

Under salinity and environmental management. 56./plants of PINSTECH.04. Government of Sindh. Pos* harvest treatments and development of seedless kinnow were the major areas of study under food crops. and Mercury was performed using the Nd: Yag laser as a pump for dye laser. Mustard canola. The proposal for its release has been prepared for submission to the Technical Sub-Committee for the Approval of Varieties and Techniques. Detailed surveys and experiments were carried out to find out causes of wheat. Availability of N to rice.628 smear samples. Cadmium.Laser Applications One hundred and fifty (150) units of Laser land leveler (LLL) were assembled and handed over to different public organizations and private farmers. oil seed and ricé crops pathologies. sugarcane borers. one analogue output. 75 mCi). 30400 were also supplied to nuclear medical centers.500. For Integrated Pest Management of various crops. PARR-2. efforts were made to lower the losses by carrying out studies to eradicate cotton pests.-12Vand+5V on board. NCD. four digital inputs/outputs. a new high yielding. unipolar & bipolar input range. 5146100 was dispatched to different nuclear medical centres in Pakistan. disease resistant wheat variety developed by NIA. 400 air samples and 162 water samples were also collected from the halls of PARR1. +12V. PARR-2 and labs. Personnel radiation contamination monitoring services were provided on about 16. P-application methods. 118000 and 4 consignments of MIBG-111I (19. About 16. For Bhittai. The NIFAAnnual Report 2003-2004 Computers A general-purpose interface module for data acquisition and control through parallel port for industrial 2 . Applied Health Physics Technical improvements were suggested for safe packaging and disposal of radioactive waste from various places of 131l plant. whereas supply on complimentary basis was 1885 mCi. various crops were screened and some income generating activities were devised for farmers. and IPD. Plant physiology and salt tolerance studies continued involving screening of salt tolerance varieties in rice. rice borers. mutant variety of mustard developed in Pakistan is canola quality and has the potential to replace the noncanola indigenous varieties.600 were also supplied. while Induced Systemic Resistance was also tried for certain crops. feed irradiation.010 radiation/contamination surveys were conducted in different labs. being the first ever. Radioisotope & Radiopharmaceutical Cold Kits Production Regular supply of sodium iodide (131l) consisting of 458 consignments (94750 mCi) worth Rs. 9 consignments of sodium phosphate ("P. 9154 vials of eleven different cold kits worth Rs. Small Angle Neutron Scattering instrument for defect studies of materials installed process and laboratory automation has been developed. Total supply of radioactive products was 94845 mCi. Rhizobiology were the topics studied for plant nutrition and soil biology.463 occasions to different radiation workers. wheat and sunflower.5 mCi) worth Rs. of IPD etc. 8 single ended analogue inputs of 0 to 10V. worth Rs. AGRICULTURE & BIOLOGY Development of new varieties through mutation breeding for crop improvement was given top priority at all research centers.51. tillage practices. Food.500. 24. trials were conducted at farmer's fields. is being tested on progressive farmer's fields as well. worth Rs. fruit flies and other economically important insects. worth Rs. 53. trainees and visitors at PARR-1. A candidate high yielding variety "Khirman" after evaluation in NUWYT. Two-step laser spectroscopy of Zinc. 8-bit resolution. 8.00. This module offers. Mustard variety "NIFA-Mustard Canola" was approved by the NWFP Provincial Seed Council for commercial cultivation in the rain fed & irrigated areas of NWFP.

Under industry. A new vaccine has also been sold in the market for the Hydropericardium Syndrome of poultry. Linear accelerators. Bioprocess Technology. heavy metal removal. Health Biotechnology and Environmental Biotechnology remained involved in research work related to Agriculture. Remote sensing. Quetta last year. genotaxic effects. Bioremediation. Kohat Plateau. geophysical surveys. Darband. neotectonic and siesmotectonics studies were also carried out for PAEC establishments. Chakdara. Mansehra. tissue culture. etc whereas detailed exploration has been undertaken at Shanawah depositsite. plant genomics. obtaining planer. Industry. chloroplast biotechnology. extremophiles were important topics of study. protein pharmaceuticals and chromosomal abnormalities were carried out under Health Section. During 2003-2004. characterization of various industrial enzymes. which are providing latest and state of the art facilities for the diagnosis and treatment of various diseases including cancer. was another favourable target where preliminary exploratory drilling was continued. The working areas include Bannu Basin. SPECT and whole-body images of the diseased organs using gamma cameras. genetic engineering.Under animal health and reproduction. For environmental studies. PGPR associations and biocontrol of plant diseases. 192. NUCLEAR MINERALS Atomic Energy Minerals Centre and its Regional Exploration Offices at Peshawar. work was done in molecular virology. development of several RNAi constructs. molecular diagnosis remained the focal point of research. Karachi & Quetta continued prospection and exploration for nuclear minerals & multimineral programme as planned. cloning of endoglucanase gene. BIOTECHNOLOGY & GENETIC ENGINEERING Six Divisions of NIBGE. related applications of nuclear technology are of paramount importance. Industrial Biotechnology. Swat. Rustam. for its uranium occurrences. Baluchistan was designated as REO. Major activities ha-'e been prospection and exploration for uranium alongwith minor activity of fluorite exploration. molecular biotechnology of enzymes. Salt Range. production of different biochemicals. HDR remote after-loading machines and Superficial appliance etc. NUCLEAR MEDICINE Pakistan Atomic Energy Commission is engaged in the application of nuclear technology for the socioeconomic development ofthe country and in the wake of growing incidence of deadly disease cancer. gaotechnical drilling. fossil fuel biotechnology. For agriculture. where geological. wastewater treatment. Kirthar Range of Sind and Eruptive Zone of Baluchistan. Tarbela. Hazara Granitic Complex. The activities in the laboratories supported the field activities by providing various chemical and mineralogical analyses. Malakand. Where as treatment was provided to more than 135. Manchar Formation in the Kirthar Range was studied in geological detail to understand the nature of uranium mineralization. Nizam Pur Basin. namely Plant Biotechnology Biofertilizers. PAEC has established 13 centers (including INOR Abbottabad recently inaugurated in May. Prospection work has been done in Kohat Plateau. etc. Health and Environment. 8000 vials of NIAB-HS-vaccine and 1000 vials of NIAB-ND-Vaccine were prepared and sold to local markets. Deep & superficial X-ray machines. Kohat Plateau. 2004) so far.000 patients using Cobalt-60 teletherapy units. Studies on typhoid. Mardan. radiometric investigations and shallow reconnaissance drilling were done in the Tuftan anomalous zone. Attock Basin. solid waste management. Hepatitis B and C. health NIFA Mustard Canola veriety Annual Report 2003-2004 3 . ^-thalassemia. Detailed exploratory drilling was started at Shanawah site of Bannu Basin in the 4th quarter to estimate reserves in the RAR category.000 patients were diagnosed by the Nuclear Medicine Departments using short lived isotopes.

Attock Refinery Limited (ARL). All the 32 successful graduates from this batch joined their respective places of posting in the last week of September 2003. Khan Research Labs. Oil & Gas Development Corporation ( O G D C ) . Chairman. Thirty-three trainees of the batch-2 (Session 2002-2003) of Post Graduate Training Program joined KINPOE in the last week of August 2003. Chashma Nuclear Power Plant (CHASNUPP). Ultrasonic Testing. 2003 to 6th October. 18. IT (2001-03) of CTC passed out on 1809-2003.Sc. 2003 to September. Pakistan has been elected as member of the Board of Governors of the International Atomic Energy Agency (IAEA) for a term of two years beginning from September. Fifteen students (10 Selffinanced + 5 Fellows) successfully completed the course. Pakistan Atomic Energy Commission attended as invited Speaker the World Nuclear Association (WNA) Annual Symposium held in London. CPC. SUPARCO. Japan and also undertook Technical Tour to Genkai Nuclear Power Station. SCIENTIFIC AND ENGINEERING SERVICES The SES Directorate manufactured and supplied jobs worth Rs. Sustainable Energy Development and other N-technology related fields. Pakistan Navy. Pakistan Steel. The Directorate exported mechanical equipment worth Rs. Radiography Testing. WAP DA. 2005. 350 millions are under design. Germany.Weapon Complex (AWC). etc. The 1 st batch of Master of Science in Information Technology. Dr. N a t i o n a l Development Complex (NDC). (KRL). other PAEC Projects. KANUPP. The jobs of contract value estimated at Rs. Switzerland as well. DESTO. Postgraduate training of 36 CHASCENT fellows batch-2 and post-diploma training of 37 O&M technicians of batch 6 has been completed. Non-Destructive Testing. Chairman. INOR. UK from 3-5 September. 2004. Final result of the batch-9 (Session 2001-2002) of KINPOEwas declared by NED University in September 2003. 2003. He also attended 2004 Spring WANO Tokyo Centre Governing Board Meeting held at Fukuoka. Parvez Butt. PAEC led Pakistan delegation to the forty-seventh IAEA Annual General Conference held in Vienna from 15-19 September 2003. the recently inaugarated 13th medical centre HUMAN RESOURCE DEVELOPMENT Thirty three PAEC scientists/engineers carried out assignments abroad in various fields including Wheat Improvement. Heavy Industries Taxila (HIT). Fiftyseven successful trainees of the batch-32 (Session 2002-2003) of the Post Diploma Training Program were relieved from KINPOE in November 2003. Q. A. Chairman. Karachi Electric Supply Corporation (KESC). manufacturing and attesting/ inspection stage. PINSTECH. The 10th batch of Post Graduate Computer Orientation Course (PGCOC-10) was conducted from 7th April. Election was held at the Forty-seventh IAEA Annual General Conference. Air 4 Annual Report 2003-2004 . PAEC participated in the Governing Board Meeting of (WANOTokyo Centre and WANO-Atlanta Centre) and Biennial General Meeting of World Association of Nuclear Operators held in Berlin. INTERNATIONAL RELATIONS Mr.334 million to CERN. He also represented Pakistan as Governor from Pakistan in the Meetings of the IAEA Board of Governors held in Vienna. M. 183 millions to Heavy Mechanical Complex (HMC).

Luxât a CANDU safety expert was also consulted.831. thickness measurement. Gross electricity generated was 78.49% respectively. Failed Fuel Activity Monitoring and Alarms Annunciation are now being performed by these systems. The Safety Parameters Display System was designed to provide concise and integrated information to assess the plant safety status continuously under all modes of operation and to guide the operator to take corrective action when so required to bring and maintain the plant in a safe state. Critical Parameters Display System was designed to provide continuous real time values of critical parameters of plant in Emergency Feed Water (EFW) room under all modes of operation to help the operator to take corrective actions in case of unavailability of main control room. To provide shutdown facility to operator outside the Main Control Room in the absence of Emergency Control Room (ECR) in the plant. detection. Canada was sent to AECL and rescans were carried out on area suspected to have flaws on advice of AECL. this license limited the power to 33% FP. Two surface indications were replicated on AECL's request. The cumulative gross generation since NPD was raised to 10.NUCLEAR POWER KARACHI NUCLEAR POWER PLANT (KANUPP) Operation and Maintenance After completion of the nominal design life of 30 years. Bearing Temperature Monitoring of Pumps & Motors. The inspections performed included pressure tube (PT) material sampling. 27 Measurement Loops and 15 Closed Control Loops have been replaced with the new l&C Systems. Reactor Power Regulation. 2004 the plant operated at an average load of about 30 MWe. visual inspection. diameter and surface roughness measurement and sag measurement of PT. Plant Regulating Computers ARC and BRC have been replaced by new l&C Systems based on PLCs. The data acquired by KNPC under remote supervision of AECL. The samples scraped from fuel channel were dispatched to AECL for hydrogen/deuterium study. installation of spring Re-licensing Outage (RLO) Activities After completion of training of 15 members of KANUPP team from AECL Canada. 2004 to June 30. KANUPP was restarted on January 22. a joint study was carried out by PAEC/ PNRA and Dr. During the period of January 22. 2004 at 22:15 hrs with a new lease of life. Fuel Channel Temperature Monitoring. The implementation. KANUPP was shutdown on December 5.52 GWh. reactor regulating computers etc to improve LOCA handling capabilities. The system was designed to provide the status of Radiological Emission & Control and Reactor Power Critically. commissioning and testing of Emergency Shutdown Facility was completed. For enhancement of power. Since then KANUPP has successfully implemented various tasks such as fuel channel inspection. To improve main control room habitability. However. inspection was performed locally on seven fuel channels in accordance with the requirements of Canadian standards. Emergency Shutdown System has been provided in EFW Room. eddy current flaw . with availability factor and gross capacity factor of 70. On completion of the regulatory requirement. PNRA issued a special license to KANUPP for restarting ofthe reactor. PT to calandria tube (CT) gap measurement. Pieces of G-12 pressure tube removed in 1993 were also sent to AECL for burst testing. AECL has submitted draft reports for comment of KANUPP.35 GWh.52% and 14. overhauling and maintenance of plant equipment. replacement. replacement of old and obsolete instrumentation and control equipment. 2002 to fulfill regulatory requirements for relicensing. ultrasonic flaw detection.

Out of this 40% was received as internal dose. The 11th IAEA Steering Committee Meeting (SCM) for ISF project was held in Vienna from 17-19 November. Installation and commissioning work of Fire Alarm system in all the plant buildings has been completed. Installation of steel wall between Main Control Room masonry wall and main steam lines has been completed up to the level of air-conditioning ducts.5 and 6 was also carried out. proposal was received from RINPO. The rehabilitation of Activity Monitoring System was completed. For improving LOCA handling capabilities.loaded louvers in the wall ofthe room facing Turbine Hall was completed. were modified and tested to confirm its integrity under severe LOCA condition. Procurement of qualified valves is in process. Emergency Feed Water (EFW) System was initially designed for providing water to all the six steam generators manually in the event of total loss of feed water due to rupture or break in Boiler Feed Water System or complete loss of power. During the 6 . has been completed. The junction boxes of safety and safety related equipment installed in the reactor building were modified to withstand the ambient condition of pressure. Two new safety cases prepared by KANUPP were endorsed by the Steering Committee. Most of the recommendations of IAEA Seismic Safety Mission have been implemented. The work involved fabrication of new sampling chambers and complete removal of old tube spools. temperature and humidity in the case of Main Steam Line Break and Loss of Coolant Accident. Proposal received from B&W Canada for conditional assessment of SG is under review. installation and commissioning of Emergency Heavy Wäter/ Light Water Storage & Transfer (EHWT) system have been completed. The tube inspection of one steam generator and visual examination of steam generator secondary side were carried out.3. In connection with follow-up campaign of KANUPP SG water lancing. Two Vertical Non Clogging Impeller Centrifugal pumps were installed. addition of redundant valves is required in Emergency Injection (IJW) system. The liquid radioactivity in the effluent released to sea was less than 3% ofthe derived emission release limit for liquid. China. while the remaining 60% was the contribution from external dose. After removal of booster rod assemblies moderator flow normalized and plant was started successfully. Hence the availability of re-circulating water for IJW system to ensure prolong cooling was made possible in case of LOCA outside the boiler room. Emergency Sump Transfer (EST) system was installed for transferring the spilled water after LOCA outside the Boiler from AD5H & AD7H sumps to Moderator Area sump. another system called Forced Emergency Injection Water (FIJW) system is required to be installed.2. All 208 sample chamber housings and horizontal sampling tubes were replaced.353 Man-Sv. installed for CCTV system in the Reactor Building. The system is capable to store eighteen tons of D 2 0 & a sufficient quantity of H 2 0 to cool the core in case of LOCA. Motor Operated Valve (MOVs) and Solenoid Operated Valve (SOVs) were completed. AMP pilot projects on instrumentation and control cables.53 mSv per person-year. Total gaseous radioactivity released from the plantwas less than 1 % ofthe derived maximum permissible release limit. In order to meet single failure criteria. In connection with on-line chemical monitoring system. Cable Gland Bushing connectors. The installation and commissioning of automatic crash cool down of boilers to cool and depressurize the Primary Heat Transport system (PHT) and to avoid any pressure stagnation in the reactor core after small LOCA. PROJECT IMPROVING SAFETY FEATURES (ISF) OF KANUPP The IAEA Technical Assistance project "Improving Safety Features (ISF)" of KANUPP continued to progress satisfactorily as follow-up of PAK-9/010 (SOK) project. As PSA level-1 result showed that automatic operation of EFW system would reduce the Core Damage Frequency by about 60%. Inspection of welds of plugged tubes in steam generators 1. due to tritium uptake. The average dose for radiation workers was 2. the operation of EFW system was proposed to be modified from manual to fully automatic which was eventually completed. 2003. Annual Report 2003-2004 Removal of Booster Head in Progress at KANUPP Health Physics and Radiation Safety The total radiation dose received by all personnel who worked in KANUPP radiation zones during the review period was 2. In this regard engineering study has been completed. To improve the functionality of the Emergency Injection System (IJW) for small LOCA scenarios. Most of the design work has been completed. field testing of Boiler Steam and Feed Wäter system panel was in-progress and the commissioning report and operating manual were being prepared.

As a part of rendering services to other national organizations. For the proposed prototype Wind Turbine Generating Station (WTGS). The average plant Anchoring of Running Transformer at KANUPP . and two surveillance audits were held successfully. Other Projects Study carried out on Nuclear Desalination Demonstration Plant has been submitted to PAEC for review. Thé performance tests on locally developed Neutron Power Instrumentation with current source and with Ion Chamber have been carried out. while a work plan for those requiring further action has been prepared and submitted to PNRA. All the outlet elbows/ bends in the high flux zone have been inspected. Expert Mission for expert review ofthe locally developed Neutron Power Instrumentation of KANUPP (NISK) was completed at IAEA Vienna from 1-5 March. CIAL acquired ISO 9001:2000 certification through M/s BVQI. regression analysis. A station instruction has been issued in the light of the expert opinions. The Equipment Performance Degradation Plan has been developed and issued for 90% of the systems. The total on line time of the TurbineGenerator was 5569 hours thus raising the on line time of TurbineGenerator to 22953 hours since connection to grid. 2004 with the participation of two KANUPP engineers. is progressively being improved to predict the useful life for each feeder. The wall thickness of 145 feeder line bends/elbows was inspected which covered more than 50% of the total outlet bends. Two locally developed N16 channels have been installed & commissioned on protective channel "A" and "B". were prepared. Nuclear Safety & Licensing Work on Allowable Outage Time (AOT) evaluation has been completed and report has been prepared and is under review. Electrical Backfitting For the project of "Replacement of 132kV Bulk Oil Circuit Breakers and 132kV Transmission Line Protection Relays of KANUPP" the contractors have been pre-qualified. An expert mission meeting was held on KANUPP Feeder Piping Integrity from 17th to 21 st May 2004. Generation License of KANUPP Application for obtaining generation license for KANUPP. CIAL h a s b e e n p r e . Design Report of Post Accident Monitoring System has been prepared. Lahore. has been submitted to National Electric Power Regulatory Authority (NEPRA). Based on the expert mission recommendations. Most of the re-licensing queries by PNRA on the PSR have been resolved 778 Annual Report 2003-2004 CHASHMA NUCLEAR POWER PLANT (CHASNUPP) Plant Operation and Maintenance Chashma Nuclear Power Plant operated safely and generated a total of 1682 GWh of electric energy from July 2003 to June 2004 of which 1558 GWh was exported to WAPDA. alongwith supporting data and documents. Provided Test/ Calibration services to M/s UEC Pvt. A selfassessment program was developed and discussed with IAEA expert in April 2001. The safety Performance Indicators (PI) has been implemented at KANUPP. NESPAK engineers visited KNPC to remove discrepancies in the final draft report on "Protection Coordination Analysis of KANUPP AC Distribution System". It has been predicted that most ofthe feeders are fit for service for another 10 operating years on the basis of wall thickness. Control & Instrumentation Study and engineering was underway for replacement ofthe remaining 136 loops both for safety and conventional C&l of TUP project. which was preliminarily performed by the expert himself. Case for administrative approval ofthe project is under process.q u a l i f i e d as "Engineering Consultant" through open national competition for plants (l&C) retrofitting of Pak-Arab Fertilizer Multan. Ltd. technical specifications of electrical equipment and interconnection scheme for connecting WTGS to KNPC Distribution System. The response of Log-N amplifier and Linear-N has been observed to be satisfactory.reporting period six IAEA expert missions were completed. Quality Management System was developed and implemented by CIAL.

14E14Bq was discharged through the stack. RCP mechanical seal inspection /replacement. II. Some of the critical jobs were contracted out to foreign contractors (Chinese) due to unavailability of local expertise. III Components.49E09 m3 of gaseous waste with gross activity of 3. The final PC-1 was prepared based on these costs and financial terms.82E08Bq was discharged through the discharge canal to the environment after dilution with the circulating cooling water. A comprehensive job list was prepared by CHASNUPP with a total of approximately more than 3000 jobs including preventive/ corrective maintenance. 2004 to assess the operational safety of the Plant. After various rounds of negotiations with Chinese organizations. Report of the OSART Mission to CHASHMA Nuclear Power Plant has been received at CHASNUPP. replacement of guide posts over the storage rack of lower core internals in refueling pool.14% and 63. Maintenance of High Head Safety Injection Pump / Motor. separation of Hydraulic Oil Supply to WCP check valves. which is well below the limit. The Second Refueling outage (RFO-2) of CHASNUPP started from 15th April. The total outage duration was nearly 134 days including the longest outage from 15th April. 360 drums of solidified solid waste were prepared with a maximum surface dose rate 50. All the events are of INES level 0. removal of Irradiation Surveillance Material for testing RPV. The average dose received per person was 0. Auxiliary Transformer & Step Down Transformer. Main Transformer. Modification of condensate Booster Pump-B motor foundation and Steam Generator blow-down Pipe Lines to facilitate Sludge Lancing and modification of Diesel Generator EEG401DG as 1E source for train B after failure of EEG001DG and EEG801DG as 1E source for train A.124 mSv. Quality Assurance QA Division conducted internal audits of Electrical Maintenance Division. QA engineers witnessed about sixty (80) periodic testing tests during reported period and participated in 388 control points during RFO-2 including control point over manufacturing of fuel for CHASNUPP at YFP China. modification in Fuel Conveyor Car to avoid damage to thimble plug assemblies. Emergency Diesel Generators. Main Generator Slip Rings by using special lathe machine type tool. 99 surveillance deficiency reports and 68 NCNs/NCRs were issued including 04 NCNs to YFP. Safety and Health Physics During the reported period. Some major modifications included addition of 02 RPV Guide studs and storage stands in reactor building to facilitate lifting of lower reactor internals. 8 Annual Report 2003-2004 . and Radiation protection section of S & HP division. Technical Support. (ii) preparation of the feasibility report of the C-2 Project for the EXIM Bank of China. So far 225 events have been encountered and 96 were reportable to Regulatory Authority. ISI. Major maintenance jobs performed during reported period (including RFO-2) included replacement of Rod Position Detector for RCCA A1-3. the Plant experienced a total of eleven outages including planned and nine unplanned outages. plant cost. During the OSART Mission. l&C Maintenance Division. Plant Outages During the reported period.capacity and availability factors were 59. PAEC establishments were also awarded contracts for carrying out certain jobs. Auxiliary Feed Water Pump / Motor. 2004.29. IAEA Osart Mission An Operational Safety Review Team (OSART) mission from IAEA visited CHASNUPP from January 12 . ECT of In-core Instrumentation Thimble Tubes and ISI of Turbine Cross-Over Piping.4% respectively. 2004. Containment Spray Pump / Motor. Eddy Current Testing (ECT) of Steam Generator Tubes. Major ISI jobs included In-Service Inspection of RPV and Reactor Internals. In this regard two major activities were: (i) preparation and submission of the final PC-1 of the project in April 2004. CHASNUPP organization practices & performance were thoroughly reviewed as per OSART standard practice in the areas of Management. 11031 m3 of liquid waste with gross activity of 1. Maintenance. Operation. Main Feed Water Pump / Motor. Fuel Handling equipment & tools. Chemistry and Emergency Planning Preparations. ISI of Nuclear Class I.2 pGy/h. Organization and Administration. implementation of design modifications and surveillance tests. APPLIED SYSTEMS ANALYSIS GROUP (ASAG) ASAG continued its contribution in planning of the C-2 project. when the reactor was shutdown for 2nd Refueling Outage. Radiation Protection. modification in mechanical support structure of In-Core Instrumentation System to facilitate thimble tube withdrawal. 2004 to 24th July. The project was approved by Major Plant Modifications Refueling outage opportunity was utilized to implement a number of design modifications to enhance the safety and reliability of equipment/ systems. testing and preventive maintenance of Main Generator. A detailed program has been chalked out at CHASNUPP to implement OSART recommendations and suggestions. fuel cost and financial package for the C-2 project were finalized. 167 drums of low level miscellaneous dry waste were prepared with a maximum surface dose rate of 430 ^Gy/h. A total volume of 2. Events and Reports During the reported period a total of fifty two events were encountered out of which nine were reportable to Regulatory Authority.

participations in C-2 activities were also made with regard to preparation of comments on technical annexure. Assessment of heat generation rate in the multi layered primary shield of Transients and Accident Analysis Severe accident. ASAG contributed in preparation of the application for the license and tariff petition. Condition monitoring of critical rotating machines was done for CHASNUPP prior to second RFO. A case study was initiated to assess cost of fossil-fuel based power generation with sequestration of C0 2 emissions compared to nuclear power option.4. for CHASNUPP was analyzed by using the computer code MELCOR 1. Station Black Out (SBO). CHASNUPP applied to the NEPRA for issuance of generation license for C-1 and determination of its bulk supply tariff. Radiation Shielding Design Evaluation of Fast Neutron Flux Spectrum in a PWR core using discrete ordinate code and calculation of Nitrogen-16 specific activity in Primary Coolant Loop was completed. participation in activities of C-2 with regards to core design. Analysis of small LOCA using best estimate was completed while preliminary computation of Peak Containment Pressure Evaluation Following a LOCA for different reactors was also completed. Institute for Nuclear Power and Karachi Nuclear Power Complex entered into a Memorandum of Understanding (MOU) for joint technical activities. aimed at continued safe operation of KANUPP. diagnostic services were provided to CHASNUPP for dynamic stress measurement on steam turbine cross over pipe during load ejection tests. The Group has been assisting IAEA/ICTP in capacity building in the area of energy sector planning. In this regard. Severe accident analyses based on double-ended cold leg break were completed. ASAG contributed in the economic and financial analysis of the project highlighting the importance of the project for the over all development of the electricity sector of Pakistan. Critical heat flux corelations used in computer program RTHP were analyzed.the ECNEC in July 2004. In addition. Multi-group shield design analysis of PWR reactor by discrete ordinate methods was completed. contract articles. The Group is participating in the cocoordinated research program of IAEA on nuclear power planning. and review of contract of fuel supply. RCS source term calculation in PWR using P C A C P c o m p u t e r code and calculation of component source term by using ACT-1 computer code was accomplished. Furthermore. INUP continued to provide requisite technical support to Chashma Nuclear Power Plant in In-Core fuel Management. INUP also assisted CNPP and HMC-3 in the diagnostic of machinery malfunction by performing vibration measurements on their moving parts and components. Analysis of flux map data of CHASNUPP at various EFPDs. Operational Core analysis and Safety Analysis and participated in the second Refueling Outage (RFO) of CHASNUPP. etc were carried out. 9 Annual Report 2003-2004 . Gamma Spectroscopy Equipment at INUP INSTITUTE OF NUCLEAR POWER (INUP) INUP continued work on analytical engineering studies related to the Pressurized Water Reactors (PWRs). The working team prepared an interim report on the work carried out during the first year. The Group initiated a country study under IAEA/RCA project on "Role of Nuclear Power and Other Energy Options in the Competitive Electricity Markets". safety analysis and thermal hydraulic analysis and computaion of core physics parameters based on energy loading. Preliminary computation of Peak Containment Pressure Evaluation following a LOCA for different reactors was done. Core Design and Fuel Management Various issues related to In-Core Fuel Management (ICFM) were routinely reviewed with CHASNUPP.8.

Burnup measurements of PARR-1 spentfuel elements were completed. Fuel Performance Analysis based on Coolant Activity data for identifying fuel failure in the core of 300 MWe CNPP was completed as well.for regular vibration monitoring of critical machines in the plant for early fault detection. A review study of Urania (UOj)Gadolinia (GdjO. shafts and blades of process machines. aimed at appreci- Expert software was developed for accurate detection and localization of loose parts. Vibration monitoring services were provided to CHASNUPP for dynamic stress measurements on steam turbine cross over pipes during load ejection tests. ating its use for controlling reactivity problems was accomplished. Detailed design of Gamma Scanning System for burnup measurements of CHASNUPP's spent fuel was completed and submitted to the plant. Nuclear Measurement Laboratory at INUP PWR by alternate methods was also completed.7 code was completed. Experiments were completed on vibration control of motor pump assemblies and diesel generator using different isolation mounts and pneumatic dampers. qualified and handed over to the plant after RFO-II. Fuel Mechanical Design A study of fuel requirement without reprocessing for 300 MWe CNPP was completed. The indigenous Plant Reactivity Meter developed at INUP for CHASNUPP was tested. Laboratory Facilities INUP entered into service agreements with CHASNUPP & KANUPP 10 Annual Report 2003-2004 . Structural analysis of the lower end structural part of CHASNUPP Fuel assembly known as the Bottom Nozzle based on finite element techniques using ANSYS 5. Work is in progress for establishment of a workshop for dynamic balancing of rotors. Analytical work based on finite element techniques using ANSYS 5:7 c o d e for S i m u l a t i o n of CHASNUPP Fuel Assembly Bottom Nozzle Perforated Plate Test was also completed. Vibration diagnostic survey of CHASNUPP's critical machines was made prior to RFO-II for condition monitoring of data of plant equipment.).

conventional state feedback control. This includes classical control. More work is required for the data in the higher energy region which is hampered due . Regarding the radiological consequence analysis for accidental releases. 560 capsules containing different samples were irradiated. topographic data of US-GS for Pakistan-region was acquired and digital map of Pakistan was prepared and compared with the map of Survey of Pakistan. The Pb (111) film shows stability upto 150 K above the normal melting temperature. Computational Physics The effect of ballistic mechanism on the phase diagram of catalytic oxidation of CO has been studied on Pt (111) surface. 98 for its operation in open end ofthe pool and results were submitted for use in an experimental setup regarding non-destructive testing of faults/defects in materials by RIAD. for subsequent use in plume dispersion modeling. core # 98 was assembled by replacing four control fuel elements. Presently the case for the final approval for the operation of PARR-1 mixed fuel core is under review by PNRA. In the linear dispersion relation. the production of Co2 started until a first order transition stopped the catalytic activity. The width of the soliton also depends upon the direction of propagation. Quality of reactor vessel/pool water was maintained up to the standard. The role of ion precipitation from magnetosphere to the upper ionospheric region has been analyzed in the low frequency perturbation regime. dipolar vortex structures associated with these perturbations have been studied. optimal control and state feedback assisted classical control. RESEARCH AND DEVELOPMENT Plasma Physics A theory has been developed to describe. a paper has been published in Phys. 97 of PARR-1 completed its operation life leaving insufficient reactivity to operate the reactor. Technique of molecular dynamics simulation has been applied to study superheating in Pb (111) thin layer embedded in AI (111) matrix.5 MeV was carried out both by experimental as well as theoretical approaches. The amplitude and 11 Annual Report 2003-2004 Reactor Physics In connection with the use of less burnt HEU fuel elements along with LEU fuel elements. PARR-2 was operated for 143 hours irradiating 1242 samples. As soon as the CO partial pressure departed from zero. The two ion component plasmas have also been studied. The steady reactive window is observed and under specific conditions the continuous transition disappeared. in a simple way. The thickness of the film has little effect on the melting temperature. the time evolution of non-equilibrium incompressible plasma to understand the mechanism of magnetic field generation taking into account the dynamics of both electrons and ions. five different conditions have been studied. which is consistent with the experimental observations. The results upto 2 MeV are in good agreement with the reported values. The theory has been applied to laser induced plasmas and the estimate of B-field turns out to be in agreement with the experimental observations. the width of the nonlinear SSAW reduce with the increase in the concentration of positrons in electron-ion plasmas. Rev. thermal hydraulic and safety analyses of a selected core were carried out and got reviewed by INUP. Reactor Experiments Experimentation has been started to measure the reactor kinetic parameters for PARR-1. Work has been started on the development of technical capabilities and computer software for analyzing the meteorological data Fast Neutron Physics After the completion of studies on thermal neutron capture crosssection and resonance integral of 1 "Tb(n. Calculations were performed for 3group fluxes around PARR-1 LEU core loading No. It is found that the electron density dips of SSAW are formed in the super Alfvénic region. Work for the development of nuclear reactor control system has been initiated. Forthe ballistic flight of "hot" oxygen atoms. Low frequency electrostatic and electromagnetic modes for dusty plasmas have been investigated.PHYSICAL SCIENCES AND ENGINEERING NUCLEAR SCIENCE AND ENGINEERING Reactor Operation During the period under report PARR-1 was operated for 550 hours including 23 continuous operations of 12 hours duration each at full power. several core configurations were analyzed to achieve required excess-reactivity and shutdown margin. Various linear limits that allow the coupling of electrostatic drift waves with the dynamics of dust grains have been discussed. Core No.g)16°Tb. Detailed Neutronic. coupling of Alfvén waves with acoustic waves and drift waves has been found for electro-ion plasmas in the presence of stationary dust. In the nonlinear case. The analysis of data for the re-estimation of absolute intensities of 56Mn in the energy range of 50 keV to 3 MeV and then upto 8. Nonlinear solitary structures of an arbitrary slow shear Alfvén wave (SSAW) in ideal electro-positron-ion plasma have been investigated. Therefore. Ion temperature gradient instability has also been studied in the astrophysical dusty plasmas. in collaboration of INUP.

Dy. Therefore. Mössbauer study on another permanent magnetic system.. Ti-Zr liquid phase has been identified by S E M / E D S analysis. Fe. and Ni. Interesting results regarding spin freezing due to Fe doping and thermal cycling effects in La^ CaJVInOj (LCM) materials have been observed. Ba) have become subject of intense research because of their fascinating and often competing properties like metal insulator transitions. Milling up to 50h did not produce composite. First report has been submitted to the Pakistan Science Foundation. Standard spectrochemical analysis technique. Ba. so further milling would be performed. 1050 and 1150 "C for four hours under the Pakistan Science Foundation project. rials ALaMnFe0 6 (A=Ca. ferromagnetic and antiferromagnetic alignments and charge and orbital ordering. He*. The application of the weak magnetic field (<10 mT) on the target surface resulted in remarkable increase in the intensity of atomic and ionic emission lines. the ion-beam from highly volatile liquid vapors has been generated.IZnxFe204 systems. Hc. From the XRD results. %) of other elements like Ga.to problems in the gamma ray detection system at Neutron Capture Gamma-ray setup in the Reactor Hall (PARR-1). Solution energies are calculated to predict the preferable site for dopant ion and charge compensating mechanism is also investigated. Nd. Electronic and Magnetic Materials Nd-Fe-B permanent magnets are outstanding in their magnetic properties like intrinsic coercivity and large energy products (BH).B6(x=13-19) is in progress. Major phases in the diffusion zone are characterized as Ni2Zr3 and Zr(Ni.. High energy ball milling of Al-5wt% SiC is carried out to form the composite materials. Bonding of Inconel 625 alloy was carried out in the presence of Zircaloy-4 interlayer at 1050 °C and 1100 "C for 3h. for the first time at PINSTECH. Bonding was not successful at 950 °C due to less diffusion of constituents and at 1150 °C due to the melting of samples. These investigations were carried out in collaboration with Korea Atomic Energy Commission. Potential parameters have been derived which accurately generate the crystal structures of these materials. magnetic remanence Br and intrinsic coercivity. crystallizing in the space group Fm'm. it was observed that these materials have two phases. rich in Cr and Mo were observed on both sides of the diffusion zone.„ AxMnO. Improvement in the properties of meltspun Nd-Fe-B ribbons and magnets prepared from them has been sought via introduction of low concentration (<5at. The X-ray diffraction investigations have revealed these materials to be cubic. has been applied to the spectra to determine the effect of magnetic field on electron temperature and density. and Nb in the base alloy Nd18Fe76B6. Si Neutron and X-Ray Diffraction The IAEA TC project PAK/4/044 has been successfully completed. Ar*. Kr+ and Xe+) bombardment has been studied. paramagnetic.. Characterization of nano-crystalline titania by employing X-ray and neutron diffraction as well as small angle neutron scattering techniques has been completed. 5 at PARR-1 and will be utilized for defect studies of materials of interest. Computer simulation techniques are being employed to investigate the structural properties of La^S^VO. based on the assumption of local thermodynamic equilibrium in the arc plasma. due to the low Curie temperature of NdjFe14B. Nd2Fe14B as major phase and Nd-Fe paramagnetic phase as minor phase. The Mössbauer results indicate that the net magnetization of the base alloy decreases due to the substitution of these elements. However. The polycrystalline aluminum and graphite targets have been bombarded with 5-10 keV Ar* ions and effect of the magnetic field on ioninduced atomic and ionic emission from these targets have been investigated in the 180-500 nm wavelength range. Ion Physics The photon emission originating from the sputtering of polycrystalline aluminum surface under 1-10 keV ion (H+.Fe)2. Under this project. decreases rapidly with the increase of temperature. a small angle neutron scattering instrument has been designed. Phases in the diffusion zone of Al-Fe-Ce and Zircaloy-4 were characterized by XRD as AIZr2 and AI2Zr5.. The diffusion barriers. Sr. The measured photon emission yields from 3d 2 D 3/2 resonance transition of sputtered excited Al atom and the calculated nuclear stopping powers have been compared. (where A=Ca. XRD indicates mixing of SiC with Al and reduction of particle size. The perovskite manganites La. Work on the synthesis and characterization of double perovskite mate- 12 Annual Report 2003-2004 . Electron Microscopy Diffusion bonding of Zircaloy-4 with SS-316 in the presence of Ti interlayer was carried out at temperatures 950. developed and installed at beam tube No. 1000.. These results show that thermal cycling in charge ordered LCM is due to disorder induced by doping at the La sites. After necessary modifications in the indigenously designed and fabricated Duoplasmatron ion source. the increase in coercivity occurs at the expense of magnetization. Sr) using X-ray and neutron diffraction techniques has been completed. Formation of brittle intermetallic compounds has been reduced considerably. The results demonstrate that elastic collision play the major role in photon emission.

a) 13 Annual Report 2003-2004 . These results favour the idea of exploring the area in detail for any possible occurrence of uranium deposit. In order to study the effects of possible radon and neutron background at SLIM experimental site CR-39 plastic track detectors have been exposed in the building from May 2002 to May 2003. Nuclear Geological Studies Samples of phosphatic nodules collected from the sandstone of Nilawahan Group near Chakwal were studied using fission track technique for the determination of uranium concentration. In connection with the setting up of a laboratory for prolonged etching of solid state nuclear track detectors. bulk etch rates have also been deduced from the slopes of the lines fitted to the plots of track diameters versus etching time. A special type of clustering source has been designed. Track etch rates in CR-39.7 MeV/u) U+Au using mica track detector has been completed. The reactions of interest are 10SCd(n.25) Bq/m3. At present the work on Cd. Moreover. This is comparable with the value of theoretically calculated total reaction cross-section 4641. These are in powder form and have been verified by using HPLC and by dissolving in toluene. The detectors placed for the measurement of neutron background have also been analyzed. Gilgit. 1 "Pd (n. The kinematical analysis of the first step of the heavy ion reaction (16. " C u and . Sun arid Venus were collinear. " Z n (n.5 MeV/u) 132Xe + Bi using CR-39 track detector has been calculated which came out to be (4587±830) mb. The projectile fragmentation in heavy ion reaction (158 A GeV) Pb + Pb is being studied using CR-39 plastic track detectors by measuring the track parameters of two detectors choosing one detector placed before and one after the target in the stack. having energy dispersive system as an attachment. Pd.p)64Cu and a°Zr(n. It has been revealed that the initial mass asymmetry of the system (0. etched in above-mentioned solutions kept at three different temperatures. It has been observed that the neutron fluence found by the method mentioned above was in agreement with the actual value within the experimental errors. Nuclear Interaction Studies The heavy ion interactions at energy < 20 MeV/u are usually used for the study of nuclear properties and r e a c t i o n m e c h a n i s m s at low excitations. the installation of a heating system for the etching solution based on oil circulation in etching bath driven by pumps has been made. while the flow in opposite direction leads to four-pronged events. Scanning electron m i c r o s c o p e (SEM). 103 Pd.64) lO^cm^h" 1 Charge Particle Accelerator 100 keV charged particle accelerator designed and made for GC University Lahore has been installed in the newly established Nano-technology lab. was used to characterize the carbon molecules. Uranium concentration in these samples ranges from (434 ± 39) to (964 ± 81) ppm with an average concentration of (690 ± 62) ppm.09) is enhanced through a flow of matter from target to projectile in the case of three-pronged events.0Y respectively. The search for light magnetic monopoles (SLIM) project is being carried out with international collaboration. After etching and scanning all the detectors the radon concentration in the environment of experimental setup has been found to be (10 46+6. and Zr for nuclear reactor production feasibility studies of'"Pd. The preliminary measurement of the cosmic ray neutron flux was found to be(5. were deduced from the slopes of the plots of track length versus etching time.7 mb. has been established. Some fullerenes CJC70 on macroscopic scale have been synthesized and characterized. five new samples of CR-39 werf exposed at PARR-1 for five different neutron fluence values. A mobile astronomical observatory was made to observe the Venus motion while it crossed the Sun. An experiment was performed to characterize carbon molecules/clusters at the tip of the cathode during the dc arc discharge. Centre for Advanced Studies in Physics (CASP) premises and inaugurated. Track Detectors Applications A new method of determining neutron fluence for reactor neutrons based on the change in transmittance of light through neutron exposed etched track detectors was developed previously at Physics Research Division (PRD). These cylinders have been encapsulated in quartz tube to visually observe the d i f f e r e n t p h e n o m e n a o c c u r r i n g in t h e regenerative sooting discharge.pn+d+np) 103Pd. An experimental set-up has been made at Koksil. Nuclear Cross-section Studies Nuclear reaction cross-sections measurement work on medically important radionuclides will continue. when earth. one acting as hollow cathode and other as hollow anode. XRD patterns were also taken to correlate the molecular structure with SEM study. The special feature of this source is the graphite dual hollow cylinders.Carbon Based Nano-technology Carbon based nano-technology lab. is in progress.34±0. Experiments were performed to determine the etching characteristics in CR-39. for the fission fragments etched in 6M NaOH solution containing different concentrations of Na 2 C0 3 . Zn.p)S0Y respectively. In order to confirm the authenticity of the method. Th^ function of this apparatus has been successfully tested. The experimental total reaction cross-section of heavy ion reactions (14.

Isotope and chemical data of groundwater samples from Quetta Aquifer has shown that the hard-rock aquifer and the alluvial aquifer were well connected and that the age of groundwater was more than 50 years. The preliminary data of isotope ratio analysis has been obtained. The concentrations are less than the maximum acceptable concentration levels of 9-30 mgL \ Fission track dating studies on Jawar carbonatites of Sillai Patti area were performed. being less expensive and stable.9 Ma) on zircon from the nearby Sillai Patti carbonatite. and FeSO«-EDTA) in wheat flour cooked into Chapatti. Erivironmental isotopic analysis of shallow and deep groundwater samples from ShorkOt area was carried out for management of saltaffected lands and brackish waters in Päkistan. This age is close to the fission track age of (32. This relationship confirms that the fission-track apatite age of this study is the emplacement age of the Jawar carbonatite.26 ±0. rainwater samples collected from selected stations with the collaboration of WAPDA. Water and gas samples were collected from different geothermal manifestations at Tatta Pani (AJK).1 ± 1. This strongly suggests the occurrence of an Oligocene alkaline magmatic episode within the region. Samples were digested and sent to Baylor College of Medicine Houston. Leavened Chapatti and Nan was evaluated. Chakwal and Northern areas to rank the potential of these geothermal sites in Pakistan. Tritium contents of water samples collected from production well indicated that there was no quick channel connecting the injection well and that the production well and the waterflood was sweeping a large volume. Isotopic and chemical data obtained from analysis of river and HUH !«M8WMî!*IIfi Life Sciences A total of 155 1JC urea breath tests (UBTs) were p e r f o r m e d o n commercial basis in the Breath Mat Laboratory at NORI. USA for Isotopic ratio analysis of "Fe/ "Fe. Interwell tracing of water flood using tritiated water was performed for enhanced oil recovery studies at Fimkassar Oilfield. Taunsa confirmed that there was no movement of lixiviant along the dip.Blood Samples for "Fe/58Fe Isotopic Ratio Analysis The neutron induced fission technique was applied for the estimation of the uranium concentration in drinking water samples collected from Skardu city. Northern Pakistan.6) Ma was obtained.1) Ma on muscovite from the same dyke of the earlier workers. For the project "Iron fortification of wheat flour (Atta) component III Bioavailability".12) Ma on zircon from the alkaline pegmatitic dykes of lower Swat area and Ar-Ar age of (28. U-Pb age of (29.4 ± 1. relative bioavailability of three potential sources of iron fortificants (elemental iron. because of shorter shelf life and cost. On the basis of results it was recommended that elemental iron.1 ± 1. may not be a suitable option for nation wide flour fortification programme.6 ± 1. Geothermal fields in Northern Areas and Chagi are found to have good reservoir potential for energy exploitation. Distt. An average age of (29. Radiotracer experiments conducted in five experimental wells at In-Situ Leach Mining Project. 137Cs and herbicide contents were measured on soil and water samples collected from Rawal Watershed. Tritium analysis of 235 water samples was extended to various countries on commercial basis under the IAEA/RCA programmes. Blood samples of study subjects (boys and girls of 6-11 years) Sampling of Geothermal spring at Ziarat. Fateh Jang and Sandaymar Dam for sediment fingerprinting and assessment of erosion/associated pesticide contamination. Transit velocities of the lixiviant along the strike for different interwell distances were provided. FeS0 4 . Irrigation Department and Meteorological Dfepartment was interpreted. should be tested vigorously for fortification programme.9) Ma and a pooled age of (30. FeS0 4 and FeS04-EDTA. Radioisotope Hydrology Development of groundwater flow and contaminant transport model of Lahore aquifer" was accomplished. were collected after feeding dose to children. Chagi Annual Report 2003-2004 14 . K-Ar dates of (31 ± 2 ) Ma on biotite from the LoeShilman and Sillai Patti carbonatites.

was carried out to help promote research and development activities and to look for defects and discontinuities etc. A laboratory is being setup for "Laser cooling and trapping of alkali atoms. Feasibility studies were made to apply hydrological computer code "Visual Modflow" for groundwater recharge and groundwater quality evaluations in part ofthe Leh stream basin within Rawalpindi-Islamabad. Tension and Torsion (TT) straps of helicopters from different Army Aviation Bases have been recommended for additional flying hours. Fabricated LEU fuel plates still lack in homogeneity as reflected in radiographic examination. redox. Most of the pollutants present in tannery effluents and sewage are blocked within the unsaturated zone having clay lenses. Both the dye lasers were frequency doubled by BBO crystals. especially. hand-pumps and springs were collected from urban Non-Destructive Testing Development work on non-destructive testing techniques to qualify Low Enriched Uranium (LEU) silicide fuel for research reactors was carried out. H. A preliminary survey of noise levels in selected hospitals of Rawalpindi and Islamabad was done. graphite element of PARR-1. ceramic and classified samples from Nucleonic Annual Report 2003-2004 Collection of Soil Core samples from Kasur Tannery Area Laser Land Leveler working in the field 15 . Indus River and Karachi sea) and shallow/deep ground water were collected from Karachi Metropolis and analyzed for M S stable isotope content. 58 groundwater samples pertaining to pumping wells. dissolved oxygen. Radiographic examination of samples from NMD. Iran. Physiochemical parameters like electrical conductivity. Dimensional measurements in locally developed LEU fuel piates were e x a m i n e d critically by X-ray radiography. Islamabad to investigate impact of contaminant transport from landfills. floods. Malir River. water and soil gas samplings were carried out at Kasur and Sambrial sites to study the pollutant transport behaviour in unsaturated zone. The results suggest that the shallow aquifer system mainly hosts recharge from local rainfall.Stable Isotope Hydrology Soil. Rawalpindi-lslamabad. Based on radiographic examination. while the confined deep groundwater system hosts trapped seawater. The development. Different modules of climate model "RegCm" were studied for climate modeling of South Asia region. Two-step laser spectroscopy of Zinc. Systems Labs. infiltrated polluted river water (Layari/Malir River Water) and Indus River Water. SriLanka. of radiotracer flowing inside the pipeline and that present in the leakage water (outside the pipeline) was exploited to identify the position of leakage. Laser Applications One hundred and fifty 150 units of Laser Land Leveler (LLL) were assembled and handed over to different public organizations and private farmers. Under the IAEA project "INT/5/44". Soil/water samples from NIAB were also analyzed for "C. Industrial Research Corrosion monitoring in oil/gas pipelines using thin layer activation (TLA) technique was completed at the Pakistan Oilfields Limited (POL) and the results were conveyed to the concerned authorities. The tracer test revealed the leakage at two points. Peshawar and Gujranwala were prepared. Environmental Research Modeling strategies for different aquifers including Lahore. leakage investigation was performed in an underground cooling water pipeline at a thermal power station. an organism which indicates contamination from sewage systems. Coliform bacterial analysis of these samples showed that tube wells situated along the Lei Stream belt contain significant population of fecal coliform. "C. septic systems and sewage drains into potable groundwater environment. analytical services were extended to IAEA member states including Bangladesh. Isotopic and chemical analyses were performed on the collected samples. pH. which is beyond the permissible limits. at detectors. The vapor containment system was thermionic diode ion detector. cable from HPD. and turbidity were measured in-situ. These services include analysis of more than 1200 samples for 1 '0. 1. Phillipines. Syria.l radiotracer. of three narrow band extended cavity diode laser systems (ECDLs) is in progress. Chemical analysis of water indicates the presence of As (Arsenic) at some locations. 15N. The time lag between the arrival. z H. Using 13. mainly Rb and Gs". Korea. droughts and extreme rainfall events. Cadmium. The PCBs ofthe drivers for Isotope Ecology and Geochemistry Forty (40) water samples pertaining to surface water (Layari River. and Mercury was performed using the Nd: Yag laser as a pump for dye laser. T. Oand 2 H. Thailand and Tunisia.

2-Dimidezoloethane) and DIP (1. Some of the parameters have been studied. Microstructure also did not reveal any abnormal structure. Installation of four more units i. Gas Absorber and Fluidizers. the composite can be Process Development Studies Fluidization studies for continuous feed & discharge system in single.1%. 0. Experiments were performed with varying gas flow rates through the column loaded with powder. Electrolytic Cell.4% have been developed at NMD. 800°C and 1000°C were performed with a view to study the containment of radioactive waste. the resin is mechanically strong.2%. glass and steel were used in the studies. An increase in the tensile Special Material Studies A laboratory named "Materials Processing Laboratory" is being established in NMD and five laboratory scale units have been i n s t a l l e d and c o m m i s s i o n e d . Perforated Plates Pulse column. Corrosion Studies Detailed studies for the evaluation of mechanical properties of Monel-400 tubes of KANUPP heat exchanger were carried out. Tensile specimens were evaluated for the mechanical properties. MATERIAL SCIENCE Chemical Material Studies Adsorption studies of "Ni on charcoal evacuated at 300°C. characterized. for the effectiveness of KANDECON cleaning solution. This recovery process is environmental friendly and economical as compared to evaporative crystallization process. The regeneration parameters of PANHAP composite were established. The results indicated no sign of degradation in mechanical properties of these tubes with a passage of 30 years in service. was carried out. only very fine specks of crud remained sticking inside the tube specimens. The assessment of amount of crud in uncleaned Monel tube of steam generator # 6 was also carried out. Cu II. foreign materials especially carbon/antimony/ sulphur in all representative areas of fuselage material (Aluminum alloy).these lasers are ready and are now being assembled in a 19" sub-rack. Miniature Cooling Tower. flaws. X = CI"1. Zn II. It was observed that maximum mass transfer took place at 7 lit/min and 13 lit/min with time duration of 30 minutes in fixed and fluidized beds respectively.4 mm. 850nm. Despite a very good porosity. 0. from dilute lithium hydroxide solution was developed to recover metal. including double Pipe/Shell & Tube Heat Exchangers. I"1 and NO/1) in air as well as inert atmosphere. The weight loss by the removal of crud was 0. The probable cause of material failure could be either aging or overloading or the conjoint effect of both which had exceeded the present limits of the material strength.02 g/cm of the tube approximately. Powder Properties Measurement Laboratory is being established.e. Radiotracer batch equilibrium experiments under variable physiochemical conditions. A setup for saturation absorption spectroscopy of Cs is in progress using Toptica's DL-100 Laser system operating at 50mW. Different fractions of carbonatite and aluminium oxide powders and balls of two different materials i. discontinuities. Al alloys with variable Mg contents i.e. 1-vinyl 2-pyrrolidone-divinyibenzene copolymer was synthesized using suspension polymerization technique. The exploration regarding thermoanalytical (TG-GTG-DTA) characterization on various metal complexes. tap density and void fraction apparatus have been fabricated and tested. concentration and temperature were carried out. two and three-stage battery of fluidizers were carried out. Cd II. turbo molecular pump and KF fittings. Fluid Flow Rig. Ion exchange-isothermal super saturation process for production of Li2CO. Cesium vapor cell and Lock-in amplifier. As a result.e. Studies on bioleaching of low-grade graphitic schist ore sample were started for the extraction of uranium but the results were not encouraging because of the. Until now we have attained a vacuum in the range of 10'7 mbar in the MOT chamber using ion vacuum pump. Work was initiated to develop a process for the production of nucleargrade U0 2 from In-Situ uranium leach liquors using ammonium carbonate solutions. R & D study on the effect of Mg addition on mechanical properties of aluminium was carried out. Mixer-Settlers Battery and Miniature DMWUnitisin progress. Br1. Metallographic observation of the cleaned specimen did not show any appreciable layer at inner side of the tube. agitation time. A few units like bulk density. such as MLX2 (where M = Co II. Ni II. and Hg II.absence of pyrites in the ore. The tubes were cleaned with KANDECON solution. L = DIE (1. The resin was sulphonated. sorbate. These have been rolled down to 86% reduction in cross-section with final thickness of 1. Annual Report 2003-2004 16 . 0. pH of soptive media. Failure analysis study of crashed aircraft Cessna 402B of Edhi revealed no metallurgical defects. Assessment of crud left after the cleaning of Monel-400 tubes of steam generator # 1 at KANUPP. Porous polyacrylonitrile-hydrated antimony pentoxide (PAN-HAP) composite was synthesized on laboratory scale and was used for selective removal of sodium from aqueous lithium solutions.3% and 0. and used for the uptake studies of Cs-137 and Sr-90 radioisotopes. An ultra-high vacuum chamber for producing magneto-optical-trap (MOT) has already been developed.3-Dimidezoloprpane). regenerated to full capacity and reused. is being done in order to investigate their mode of decomposition patterns as well as stability.

irradiated using electron beam and tested for electrical.. 1 to 2 mm. Pellets of different compositions were prepared and sintered. whereas relatively coarse pores were observed at particle boundary. After establishing the sintering parameters. Polymer Processing and Radiation Studies R a d i a t i o n c r o s s . grains of 6 to 8 mm were present while the if size of the grains in other area was only > . Two types of pores were observed. Work on magnesium aluminium silicate machinable glass is in progress. A gas c h r o m a t o g r a p h y (GC) procedure has been developed for simultaneous separation of saturated Laser Welding Machine chamber was provided.strength of the alloys with increasing Mg content has been noticed. Development of these insulations makes it possible to develop nuclear power cables in which other sheath is FRPVC and insulation is made of flame retardant crosslinked LDPE. The results revealed that sintered pellets had equiaxed grains with an average size Arc Melting Furnace 17 Annual Report 2003-2004 . Blending of powder at 310 rpm for 4 hours was found to be the best of all. Materials Research Studies on surface modification of metal substrate such as stainless steel by reactive oxides are continued. Studies on hydrogen and hydride induced degradation of the mec- Fuel Fabrication Studies to produce PWR fuel pellets were continued. Some more experiments on non-isothermal oxidation of yttriadoped zirconia coated stainless steel were performed. Fine pores were found distributed uniformly throughout the sample. Pellets of fine powder with particle size of 1 to 5 mm have shown good sinterability at 2150 °C in argon atmosphere. Installation of Laser welding equipment was carried out. Alloy Development and Mechanical Testing In continuation to the ongoing project: "LEU fuel fabrication for PARR-1 ". ANALYTICAL TECHNIQUES A high pressure liquid chromatography (HPLC) procedure has been developed for separation and characterization of fullerenes in carbon soot. In some areas. arc melting parameters for synthesis of U.Si2 were finaliz e d w i t h 97% phases concentration determined by XRD.5 g/cm3. " ! ji-. Sintered density upto 2. High molecule carbon compounds namely C i0 and C70 has been separated and identified using C18 reversed phase column and UV detector. metallography of U0 2 pellets was carried out to determine the grain structure and morphology of pores. Welding studies of different materials have been initiated. The pellets were sintered in COz atmosphere in temperature range from 1000 °C to 1300 °C. mechanical. i. Some experiments on normal oxidation of uncoated stainless steel and non-isothermal oxidation of stainless steel were carried out.28g/ccwas achieved. thermal. Laser welding of stainless steel strips was done on experimental basis. Hydride layerof thickness 12 to 26 mm was deposited. Attachment of air filter and extra supply of argon gas for inert atmosphere to welding of about 6 mm.' F a b r i c a t i o n and characterization of boron carbide pellets was carried out. QA/QC work has been undertaken to assess the performance of l&C cables at KANUPP. hanical and physical properties of zirconium base alloys are being carried out.l i n k e d flame retardant LDPE/PVC based wire insulations have been developed. Results revealed that pellets sintered at 1300 °C for four hours had attained the required density of 10.. Microstructural investigation revealed that pellets contained equiaxed but inhomogeneous grain structure. flame retardancy and radiation resistance properties in c o n f o r m i t y with i n t e r n a t i o n a l standards. All the insulations passed the tests. Study for development of low temperature sintering process for PWR fuel pellets were continued.

using this instrument.448) Bq/Kg. This study documented high suspensamples each were analyzed during ded particulate concentrations the period under report. and Pr(N03)3 in aqueous were analyzed for their copper. and its trend". 114 samples of comprising vegetables.10.152) Bq/Kg for uranium. The vascular and renal hypertensive measurements of radon in the soils of Viscosity behavior of La(N0 3 ) 3 .-C4 saturated / unsaturated monitoring were hydrocarbons. around the industrial areas of Faisalabad and Gujranwala was Under the IAEA project "Air pollution Environmental Studies continued. Three sets of 17 300 minutes. Chalas. using various tetra alkyl ammonium 5R). DPTA. Spectra of 36 samples previously collected from 17 different localities with in the country were taken with HPGe detector system. Khan and Mansehra were analyzed for activities due to " 2 Th and "K. Jones-Dole equation and values of is increased and that of zinc is interaction coefficients were comdecreased in patients as compared to The project to study the ecosystem the normal subjects. using instrumental EGTA) have been incorporated to neutron activation mask high contents of iron in uranium analysis. The radioactivity measured in samples so far shows no hydrocarbons from unsaturated abnormal trends analogues using indigenously developed GC column packing Five different material (Fe 2 0 3 coated alumina species of plant columns). Ten samples of different brands of £ mineral water consumed in exceeding the WHO recommended limits. 25. Improved selectivity have biomonitorsforthe been achieved regarding separation environmental of C. radiation laboratory of PRD. provided by IAEA.241-1. 105 samples of whole blood 15 cities of Pakistan were carried out Ce(N0 3 ). The levels of radiation were found to be in the range (0. Five samples from exposed In PARR-1 and PARR-2 with black carbon contents by using different locations in each district will thermal neutrons for 2. patients. lead and cadmium IAEA. Rawalpindi/ Islamabad area ' have been analyzed for natural Multi-channel Analyser set radioactivity. be analyzed. The be in the range 22-184 pCi/l. Eisakhel.radioactivity in samples analyzed so far is under the permissible levels. (0. Sixty three samples. D. puted. (Pylon MoorAB-SR) be used as bioOctadecyl silane (ODS) reversedmonitors for the phase column has been modified to A scintillation based portable evaluation of atmospheric pollution dynamically anion-exchange column radiation monitor (Pylon Model ABlevels. and medium lived trace elements Various complexans (EDTA.321-1.I. Mandi Bahaudin.714) Bq/Kg and (118. 35 and smoke stain reflectometer (SSR). zinc. The radon level solutions has been studied as a lead and cadmium contents using in soil gas of these areas was found to function of concentration and tematomic absorption spectrometer. The results indicated that the concenless than maximum limits set by data have been analyzed in terms of tration of copper. Finding determination in various IAEA. were papers have been analyzed for their measured. thorium and K-40 respectively.9261114. previously collected collected on coarse and fine filter from 34 districts of Punjab is being during the winter season. crops and airborne particulate matter (APM) Natural radioactivity in soil samples soil samples. which is perature as a reference data. has been In connection with correlation studies salts for the analysis of essential and made operational in environmental of bloodmetal contents with cardiotoxic anions in potable water. Taxila. shows that these standard reference materials (SRMs) A scintillation based portable Radiation Monitor plant leaves can using ion-pair HPLC. Effect of temperature analyzed for short on selectivity has been completed. The gamma spectra of 9 soil samples from the areas of Gujrat. The 18 Annual Report 2003-2004 .

5 mCi) worth Rs. The study of determination of Cu. HPLC. TBA showed antagonism due to strong affiliation with HPA and Picoline-N-oxide did not show good synergism due to its solubility in aqueous solution. sodium car'bo. Tb and Tm onto PAN (1-(-2-Pyridylazo)-2-naphthol) embedded synthetic polymer has been studied. 75 mCi). 131l (1200 mCi) to RIAD. Central Analytical Facilities During this period 400 samples were analyzed on payment basis by ICP. Design work is being done for the preparation of fuel cell stack.The gamma spectra. and selectivity of sorbent has also been studied.was generated.g) reactions on the target materials. modifications and improvements of multi-element hydride generation technique for ICPOES for As. 32P (5 mCi) to Karachi University.500. 15-crown-5 (15C5). Khan University. Effect of temperature. The calculations for the concentration of 30 elements in each of 42 samples. Radiochemical separation methodology was developed for the separation of bulk of the radioactivity produced by (n. Extraction of rare earth elements (Pr. Sb. obtained for these samples were analyzed for short. CP-3800 GC. Picoline-N-oxide and TBA for the extraction of Eu(lll) with HPA from pH 2 buffer solutions was tested and it was observed that synergic enhancement was of the o r d e r of T O P O > T P P O @ Cyanex471x>1 5BC5>15C5. A new separation procedure for Cd in Iodide medium from Zn bulk has been developed using polyurethane foam packed column. products was 94845 mCi. AAS.00. 56. have been carried out.88. 1 "Cs (10 mCi) and " 7 Cs (150 mCi) to D. di-. Saturn 2000 MS in CAFD for the analysis of organic compounds radioactive Research & Developments of Fuel Cells Theoretical studies for the development of electrochemical methods for the characterization of electro catalysts for fuel cells and coupling of electrochemical instruments for potentiostatics and galvanostatic measurements for fuel cell have been done. Fe. 51. Studies have been initiated for the evaluation of fuel cell electrodes using Tafel plots. Benzo-15-crown-5 (15BC5). and Eu.18.000/= were dispatched to nuclear medical centres and two hospitals in private sector.51. Effect of various neutral donors such as TPPO. I. whereas supply on complimentary basis was 1885 mCi. TOPO.100 was dispatched to different nuclear medical centres in Pakistan. A method has been developed for the characterization of catalytic activity of fuel cell electrodes by voltammetry. 9154 vials of eleven different cold kits worth Rs. Cu. Gd and Sm) with picrolonic acid (HPA) in MIBK has been studied. Zn and Mn in soil samples collected from various ecosites of the country SERVICES AND PRODUCTS Radioisotope and Radiopharmaceutical Cold Kits Production Regular supply of sodium iodide (131l) consisting of 458 consignments (94750 mCi) worth Rs. worth Rs. supplied to nuclear medical centers. studied so far. Se and Te and development of reliable method for the determination of sub-ppb level boron in the nuclear grade steel and aluminum by ICP-OES and SparkOES. various anions and cations. Radiochemical separation procedures forZn. 10. A revenue of Rs. Selectivity of the extraction system for various mono-. A method has been developed for the coating of graphite by catalyst metals for the preparation of catalyst materials for fuel cell electrodes. 1. PAKGEN Tc " m Generator Regular production of PAKGEN 99mTc generator was continued and 206 consignments of 114355 mCi worth Rs. However. These samples were received from different centers of PAEC as well as outside PAEC.600/= were also supplied during this period.500. worth Rs. medium and long lived radionuclides. "mTc ( 5 0 0 m C i ) to PIEAS. A good synergic e n h a n c e m e n t has also been observed for the extraction of Eu(lll) with hexafluoroacetylacetone (HFAA) + TPPO in benzene from pH 2-5 buffer solutions. Various research activities include development. 20. 53. worth Rs. Ho. Cyanex 471x. 30400 were also 19 Annual Report 2003-2004 .nate (MNa) 140 mCi to R-Block. XRF. 24.000/. Adsorption Studies The preconcentration of Eu onto sawdust. tri-. Other supplies include.000 and 4 consignments of MIBG-"1I (19. AES. Rh and Ru were developed and applied during the period under report.04.79. and tetra valent metal ions was also studied. lonchromatography and GC.46. 9 consignments of sodium phosphate (32P. The yield ofthe radiochemical procedures was established by performing a series of experiments with the tracers. Total supply of GC-MS.

Radiometric analysis services were provided for 2698 environmental samples received from IPD.048 mGyh"1 was obtained./plants of PINSTECH. 431 TLDs were irradiated using 60Co/137Cs irradiation facilities. 400 air samples and 162 water samples were also collected from the halls of PARR-1. 247 pen type/alarm dosimeters. Personnel radiation contamination monitoring services were provided on about 16. PARR-2 artf Applied Health Physics Technical improvements were suggested for safe packaging and disposal of radioactive waste from various places of 1ï1l plant. Environmental/area monitoring services using TLD cards (G-2) were provided and 847 TLD cards were processed. Determination of toxic elements in Indus Basin Sediments and of essential elements in an herb named Fagonia Cretica by using AAS is in progress. at the Institute as well as on Country level. 7 neutron survey meters. Beam output measurements of 6 teletherapy units.628 smear samples. of NCD. through countrywide air monitoring network. 1742 consignments of radioactive material/radiation sources were checked and authorized for safe transportation to various places within and outside PINSTECH. IPD etc. 170 samples were analyzed for the measurement of 131l and 63 environmental samples forthe measurement 5°Sr activity. and IPD. M e a s u r e m e n t of ambient gamma dose level around PINSTECH was made and an average dose rate of 0. Radiation Dosimetry Personnel monitoring services for external sources of radiation were provided. About 16.463 occasions to different radiation workers. Thyroid scanning facility for the determination of any inhaled activity of 1311 was provided to 98 radiation workers of Iodine plant. Mobile Radiation Monitoring Laboratory (MRML) during field survey Annual Report 2003-2004 20 . PARR-2. and 9 therapy level dosimeters were calibrated and certificates were issued. monitoring and degradation of Chloropesticides (PoPs) using GC are continued. trainees and visitors at PARR-1. PARR-2 and labs. using G-2 TLD cards. 105 protection level survey meters.010 radiation/contamination surveys were conducted in different labs. About 28000 films and TL-dosimeter were processed. Enhancement of the sensitivity of Hf determination in Zirconium Oxide by AES was carried out. PARR-1. Environmental Monitoring Radiological assessment of 283 air particulate samples was made. NCD. Services of mobile radiation monitoring laboratory (MRML) were extended in emergency preparedness and off-site radiological environmental monitoring and hazard assessment p r o g r a m m e . and got analyzed for the detection of accidental/abnormal releases of radioactive materials into the environment. 36 radiation monitoring detectors. installed at various Radiotherapy Institutes in the country were carried out and reports were issued. for the detection and measurement of gamma emitting fission fragments and activation products.210 ± 0. 8. identification. Preparation of material and fabrication of glass columns for preconcentration and also radiolytic d e f r a g m e n t a t i o n of PAHS is underway. Secondary Standard Dosimetry Laboratory During the reported period. Assistance for the smooth operation and functioning of radiation emergency centers (REAC-1 & 2) was sustained. RWMG. using thermoluminescence dosimetry (TLD) and film badge dosimetry techniques.by employing AAS by various digestion procedures was completed. Separation. The services were provided on monthly basis to about 3100 radiation workers of 285 establishments. Safe operation of PINSTECH nuclear facilities was confirmed through strict radiological surveillance and environmental monitoring.

edu. This module offers. This site provides the information about the status of production in PINSTECH. Similarly. The URL given by the National Centre for Physics (NCP) for this site is: http://www. IAEA's non-conventional literature (NCL) and current contents database received weekly on floppy diskettes were also provided to the users as and when required. which is the normal practice of worm for continuous monitoring ofthe system.1A). A Web site is developed and published to reflect CMS production activities at PINSTECH. About 345 online retrospective search results were provided from INIS database on CD-ROM to the users from PAEC and other R&D organizations. Multan. which is a part Of PAECCERN collaboration. Help was extended to upgrade PINSTECH Library into National Library of Physical Sciences for establishing the Local Area Network in SID and also installed Windows NT 2K base advance Network server. The instruments were developed for use in research and development work.>i /' '««'iii CERN-CMS Production Laboratory at PINSTECH 21 Annual Report 2003-2004 . -12Vand+5Von board. Computers A general-purpose interface module for data acquisition and control through parallel port for industrial process and laboratory automation has been developed. Rahim Yar Khan. 17 radiation sources of 57Co & one "°Sr source from MINAR.Radioactive Waste Management Approx. Sadiqabad and 27 sources 132lr from JIACCO (Pvt) Limited..435 documents from INIS database. 8 single ended analogue inputs of 0 to 10V. 3 sources of 1 "lr from FFC. The SUN computer systems computation services worth Rs. Electronics and Instrumentation A safety log-linear channel was fabricated and sent to KANUPP for field testing. INIS database covers the period from 1970 to April 2004.ncp. Annual test and calibration of PARR-1 was carried out. Bore-holes were monitored 12 times to check the underground movement/migration of radionuclides from disposal area. It can also map the open ports. and a temperature alarm were made.1. 13. Controlled area/personnel monitoring was performed 10 times using area/ personnel TLDs. A delayed neutron monitoring channels is being designed for KANUPP. +12V. PARR-2 and IPD (131l plant) were collected and got monitored for any abnormal release. This module is used in the project "Design of development of an expert system for process monitoring and control".6 m3 of low-level solid waste having short-lived beta/ gamma emitting radionuclides was received for disposal.654 documents from TOC's of journals respectively. Lahore were collected and placed in an interim storage for conditioning and disposal. four digital inputs/outputs.94 CPU hours were provided during the period. 164 samples of exhaust air from PARR-1. large number of 132lr sources seeds from M/s Shaukat Khanun Memorial Cancer Hospital.478 documents from technical reports and 27. Presently software is able to capture all packets. One dc power supply (0-1 100V. Compact Muon solenoid (CMS) production center is established in PINSTECH. A TLD • . reader was studied for reverse engineering and a preliminary report was submitted.pk/PAEC1/. unipolar & bipolar input range. 8-bit resolution. Lahore. one analogue output. Commissioning of various packages such as batch object submission system (BOSS) and MySQL are completed. 1020 m3 of low-level liquid waste received from PARR-1. Software for network security is developed to monitor network traffic and possibly detect any malicious contents in it. IPD and NCD having gross beta/gamma activity of 2211 MBq was disposed off after necessary treatment. 17 kg of medium level solid waste received from IPD was placed in an interim storage for decay/ disposal. Twenty transformers and one hundred fourteen printed circuits were made for repair and development work. six personal gamma alarms. Database server and information server. Routine repair and maintenance of instrumentation and control systems of PARR-1 and PARR-2 were carried out. PARR2. @ 0.5 Million against 1503. 6. Reconditioning of four surveymeters were completed. Scientific Information During this period SDI services were provided extracting from 38.

preparation of PINSTECH progress reports and collection and dissemination of scientific information and training of technical manpower within Pakistan and abroad. universities and other research organizations within and outside the country. storage. SID has continued its efforts for speedy acquisition. Regarding the project "Upgradation/ strengthening of PINSTECH Library into National Library of Physical Sciences (NLPS)". PakAtom. 'AtomNews' 'Nucleus'. retrieval and dissemination of scientific and technical information in support of different PAEC programmes as well as other R&D organization/universities within the'country. Nathiagali Summer College. brochures and different leaflets like Saline Agriculture for Participatory Development Project in Pakistan. SID desktop publishing cell is continuously providing support to other divisions and establishments. Programme Coordination Programme Coordination Division provides liaison between PINSTECH. During this period 370 inputs were processed and sent to INIS database of IAEA. Similarly 285 documents have been dispatched to INIS to assist IAEA as voluntary contribution by Pakistan. All visits to PINSTECH including VIP and experts visits are also taken care of by this division.28 million of non-conventional literature (NCL) and over 60. more than 1. 22 Annual Report 2003-2004 . Agro Land Leveler and PAEC Annual Reports for the last two years have been published and printed in SID printing press. Internet connectivity through radio modem and Elibrary system package is near completion. It deals with coordination of all interdisciplinary scientific programmes.542 books.As SID is the national center for International Nuclear Information System (INIS) in Pakistan. the input of the research material originated in Pakistan in the area of nuclear science and technology is one of its primary responsibilities.536 backdated journals. In this respect several jobs like 'Glimpses from Fresh Arrival'. PAEC establishments. The total collection of library now stands at 35. national and international research contracts. Breast Cancer.

disease resistant. A candidate high yielding variety Sl911995 "Khirman" after evaluation in NUWYT. The proposal for its release has been prepared for submission to the Technical sub-committee for the Approval of Varieties and Techniques. Out of 9 promising rain-fed lines evaluated in replicated micro plot trials conducted at 9 sites in NWFP. a new high yielding. Wheat varieties Sarsabz and Kiran-95 evolved by NIA remained main cultivars in the province of Sindh. disease resistance and wide adaptation. Islamabad. CCRI.BIOSCIENCES AGRICULTURE & BIOLOGY PAEC has played a pivotal role in the enhancement of productivity of agricultural crops by evolving 47 new varieties. Faisalabad and Nuclear Institute for Food & Agriculture (NIFA). disease trap nurseries and disease screening nurseries were planted at NIA. Tandojam. which have brought additional benefits to the farmers worth six billion rupees per annum. Susceptibility to widely grown wheat variety lnqilab-91 is likely to be attributed to this virulence. three lines produced higher mean grain yield and showed wide adaptability. nonreplicated trials were conducted at the farmers' fields during 2003-04. In addition new hybrids were also produced using commercial varieties and salt-tolerant wild species especially Aegilops geneculata and A. trials of advanced stress tolerant and low input material were conducted at various places starting from Faisalabad up to Rahim Yar Khan. During Rabi 2003-04. pest management. namely Nuclear Institute of Agriculture (NIA). Nuclear Institute for Agriculture & Biology (NIAB).32 metric tones) of these varieties was made available to the public and private sectors. New variety Marvi 2000 was also inducted in the provincial wheat varietal system. The results of N U W Y T c o n d u c t e d at NIFA. Pirsabak. The rainfed wheat line NRL-9912 included in NUWYT-Rainfed performed very well with respect to grain yield. compared to the recommended commercial varieties. The new candidate irrigated wheat line CT-00231 completed its two years mandatory evaluation conducted on more the 40 sites throughout Pakistan. Government of Sindh. Cylindrica. NRL-0228 followed by NRL-0201. Tandojam. Eight tons of quality seed of wheat varieties Tatara and Takbeer were produced at NIFA and Pirsabak Research Farms. WRI. Regarding the production of stresstolerant-wheat through the use of agro-biodiversity. particularly in the rice-wheat area. In the advanced barani yield trials. About 5.5 tons Breeder Nucleus Seed of variety Fakhr-e-Sarhad was produced for future utilization. (27. viz. rust resistance and other agronomic traits. Peshawar showed that under normal conditions this line has produced the highest grain yield (5067 kg/ha) and ranked first. Three candidate lines having high yield. All of its agriculture centres. 7-03 and RWM-9313 were promoted in the NUWYT. SD-66. possessing high protein and dry gluten wheat variety. High quality seed New high yielding wheat line developed at NIFA 23 Annual Report 2003-2004 . The yield remained between 3000-4900 kg/ha with half the inputs used. NARC. Peshawar are working on R&D programme with the objective of tapping the latest research trends in agriculture for crop improvement through mutation breeding. etc. Faisalabad and Badin for evaluation of prevailing spectrum of wheat diseases. is being tested on progressive farmers' fields. drought tolerant. prevention of post-harvest losses and by controlling salinity and waterlogging. CROP IMPROVEMENT PROGRAMME Wheat To evaluate the yield performance and stability of Bhittai. Results indicated the appearance of new virulence capable of attacking genotypes possessing YrJT. NRL-0211 and NRL-0227 produced higher grain yield than the check varieties. From the screening nursery many genotypes have been found resistant against this virulence.

the line CMNK-262-26 produced significantly higher seed yield of 2353 kg/ha compared to the standard check variety Hassan-2k (2271 kg/ha). About 500 kg pre-basic seed of Niablrri-9 was supplied to the Punjab Seed Corporation and progressive farmers. Rice Pre-basic seed 2427 kg of rice varieties Shadab. Uniform Rice Yield Trial (aromatic) ranked 6th and 9th. The variety Chandi-95 covered 12200 ha in Sindh and earned economic return of 650 million rupees. Khan during Rabi season 2003-2004. were evaluated in multilocational yield trials conducted at different locations in chickpea growing areas. respectively. 40 healthy plants of NIAB-111. cotton mosaic. stunting and boll rots) revealed that most of these strains/progenies were highly resistant against CLCuV-old. 24 Annual Report 2003-2004 . NIAB-2000. The proposal of aromatic mutant variety Bas-15-2 "Mehak" has been submitted to the Technical subCommittee for the Approval of Varieties and Techniques. An additional annual income of Rs. Two advanced lines namely. Agricultural Research Station. approved NIAB-111 as a variety for general cultivation. this mutant produced 9% higher yield than the standard Super-Basmati. bacterial blight and stunting complex. cotton mosaic. D. and available germplasm were evaluated through grafting against CLCuV-B under glasshouse conditions while CLCuV-M under net house conditions. A new advanced mutant (nonaromatic) was contributed to the NURYT for its yield potential and stability parameters.a r o m a t i c ) and Basmati-15-2 (aromatic) have been tested in National Uniform Rice Yield Trials (NURYT) during 2003. I. 2004. to identify the genes for resistant sources. in its 29th meeting held in Lahore on April 9. varietal purity of NIA varieties has been maintained over 24ha of Chandi-95 and 16ha of Sohni at different In follow-up programme. respectively. A check variety Pb-1 gave 840 kg/ha. bacterial blight. and evaluated for yield potential and other desirable traits at NIAB campus. Khushboo-95 and Sarshar was supplied to progressive growers of Sindh and Baluchistan provinces. i. P 38-6-1 and P 52-9-2 included in the National Chickpea The chickpea advanced mutants were evaluated in adaptation yield trials at three locations in NWFP. Government of Sindh. an aromatic mutant has completed three years in the NURYT Based on three years results in the Punjab province. The data showed that both NIAB-999 and NIAB-111 possessed the trait of wider adaptability and stability for different plant attributes in different cotton growing zones. NIFA. in various trials as a candidate variety for commercial release.e. collected during 2002-03 from CLCuV-B hotspots. On the average of seven locations. and were sustained in the NURYT for next year. 45 strains / lines. 20 true to type progenies of Niab-lrri-9 and 40 progenies of Kashmir Basmati were raised. Desi mutant line CM588/99 and Kabuli mutant line CM256/99 exhibited the highestyield 1404 kg/ha and 1124 kg/ha. To continue the purity cycle and production of pre-basic seed for supply to farmers.Cotton The Punjab Seed Council. 15759 million has been generated through the cultivation of these varieties. In follow-up programme. Hybrid lines yielded in the range of 833-1750 kg/ha. These varieties cover about 58% of the total rice area in Sindh.e. IR6-25/A ( n o n . In case of Kabuli chickpea adaptation yield trial. while 6 lines gave 53-91% higher yield over the best check variety. the most promising among these has been included in DGR trials.. The varieties Chandi-95 and Sohni released by NIA were promoted to the growers of Sindh with complete package of production technology. a high yielding hybrid line P40/91 was crossed with a blight resistant bold seeded Kabuli variety Noor-91. Shua-92. Karak and Agricultural Research Station. Observations for multiple disease resistance (i. was carried out. and 6 bold-seeded and blight-tolerant Kabuli lines of variety Pb-1. The non-aromatic mutant line IR625/A secured 7th position among the non-aromatic group and Basmati-152 secured 2nd position among the aromatic group. varietal purity of NIA varieties has been maintained over 24ha of Chandi-95 and 16ha of Sohni at different grower's fields. The pre-basic seed of Chandi-95 (190 kg) and Sohni (170 kg) was supplied to government/private seed agencies and progressive farmers. Performance of varieties NIAB-999 and NIAB-111 was monitored in different agro-climatic zones in comparison with other commercial cultivars for various plant attributes. Twenty commercial varieties. New mutant lines NIAB-824. Two candidate mutant varieties viz. CLCuV-Multan. Nine blight resistant mutant lines of an exotic line Pusa 329 (Desi type). Evaluation of mutant NIAB-98. NIAB-824. To incorporate blight resistance and improved seed color. NIAB846 were bulked.

An artificial inoculation technique for the screening of mungbean germplasm through grafting against M u n g b e a n . being the first ever-mutant variety of mustard developed in Pakistan. During the adaptation yield trial comprising of 16 entries. 32. 60 kg pure seed of candidate canola hybrid line (97-1/5-1) was also produced. The line NFM-12-12 was contributed to NUYTs and it got 4th position in the trials on national basis and 1st on provincial location basis.Oilseed Brassica Mustard variety "NIFA-Mustard Cariola" was approved by the NWFP Provincial Seed Council in its meeting held on 8th October. c r o s s combinations of local and exotic germplasm were evaluated for seed yield and some important agronomic traits in three Early maturing candidate lentil variety evolved through mutagensis 25 Annual Report 2003-2004 . Thirteen genotypes were selected on the basis of earliness in flowering/ maturity and ten genotypes were selected on the basis of high bearing. inexpensive and faster. generation. Sesame Sesame germplasm (106 lines) of indigenous and exotic origin were collected and planted during summer2003. The germplasm of 156 entries was planted for maintaining its seed for breeding purposes.m o s a i c was standardized.6 million by its higher yield. Thirty-four advanced lines derived through eight different Lentil Two candidate lines AEL 49/20 and AEL 23/40 were included in Lentil National Uniform Yield Trial (LNUYT) 2003-04. During 2003-04. earliness and dwarf plant type. sets of replicated yield trials during kharif 2003 at NIFA research farm. Two hundred kilogram high quality seed of AEM-96 was supplied to progressive growers for multiplication in 2004. The results provided by NARC. Twenty-four desirable mutants were selected in M2 generation on the basis of desirable parameters. Castor bean-a high value industrial oilseed crop The NIFA-Mustard Canola. Islamabad showed that the candidate lines AEL 49/20 ranked first (2956 kg/ha) and AEL 23/40 (2903 kg/ha) 2nd in province of Sindh. Six promising lines selected from M2 were grown as M. Candidate lines were evaluated in the zonal trials at five different locations in the province of Sindh.y e l l o w . line NM-15-11 produced significantly higher seed yield of 1847 kg/ha followed by NM-45-24-1 (1833 kg/ha) as compared to the check NM98(1621 kg/ha). To evaluate the true breeding germplasm. About 180 kg BNS of Abasin-95 and NIFA-Mustard Canola was developed at NIFA for maintaining the seed purity of these varieties. is canola quality and has the potential to replace the non-canola indigenous varieties. The variety has contributed extra income of Rs. 2003 for commercial cultivation in the rainfed and irrigated areas of NWFP. NM-1 produced significantly higher seed yield (879 kg/ha) followed by NM-98 (843 kg/ha). different sets of yield trials and segregating germplasm were conducted at NIAB. The seeds of two local lines were exposed to 150 . Screening of mungbean germplasm using this method will prevent the chance of escape infection. The yield trials of the five mungbean varieties/genotypes were conducted in different locations in NWFP during kharif 2003. thereby enhancing the domestic edible oil production. 590 kg pre-basic seed of AEM-96 has been produced. One set of Mungbean Regional Mutant Multi-location Trials (RMMTs) was conducted at NIAB during spring 2004. This technique is simple. Tandojam continued gaining popularity among the growers and covered about 30% area under mungbean in Sindh. Hybridization in mungbean at NIA is being tried for the first time.400 Kr of gamma radiation for induction of desirable variability. Twelve genotypes were Castor Thirty castor lines were collected for evaluation and characterization. Moreover. Mungbean The high yielding mungbean variety AEM-96 evolved by NIA. probably as a consequence of non-preference mechanism and loss of vector infectivity.

bollworms population continued through pheromone baited traps. Dry. minimum hatch percentage was recorded in the cards placed at lower part of the plant. It has been noted that the distribution for irradiated seedlings. number of shoots per plants. viz. In-vitro cultures of 13 different banana clones were maintained and multiplied for mass production. Banana Banana clone Basrai (commercial variety of Sindh) was used as initial material and multiplied through micro-propagation. Studies revealed that irradiation enhanced the resistance in the parasitoids against insecticides as higher parasitic potential was recorded in the irradiated parasitoids than the untreated insects. Trichogramma chiionis in conjunction with pheromones. tissue culture and seed fuzz are in different stages of selection and evaluation. spotted and American bollworm moths as determined from the number of insects captured per trap/month. were skewed towards the lower heights with the lowest height of 49. dormant and healthy seeds of high yielding tomato genotypes were irradiated with different doses of gamma rays for the mutation alteration of horticultural traits and induction of male sterility.. Sugarcane NIA 98. the population of the pink bollworm moths was higher throughout the year than spiny. In-vitro mutagenesis studies in banana were initiated and micro-propagules were irradiated with different doses of gamma rays. Lentil International Drought Tolerance Nursery (LIDTN) and Lentil International Elite Nursery Early (LINE-E) of ICARDA. The bollworms management cost through the integration of pheromones. treatment as compared to control of 104. Integrated treatment of parasitoids. Maximum eggs of the predator. Similar trend in the adult emergence of the parasitoid. Releases of both parasitoids and predators at Habib Farm. several local elite tomato genotypes were crossed in triple test cross and line x tester fashions. Cotesia flevipes. The application of bio-control technology managed the whole insect pest fauna below the economic threshold level.2 to 0. Study on the effect of different auxins and cytokinins on rapid multiplication of banana was accomplished. Whereas. Hyderabad on 150 acres of cotton successfully controlled the cotton bollworms and the sucking pest complex. have been selected after extensive screening.evaluated at five different locations in Sindh. regardless of the treatment. Trichogramma chillonis was recorded. gene action and to find out best combiner lines. s p o t t e d and pink Annual Report 2003-2004 26 . Citrus Seed-lessness was induced in Kinnow Citrus reticulata Blanco with gamma radiation and a sparsely seeded mutant (5 + seeds/fruit) was developed from highly seeded parent (25 + 5 seeds/fruit). Breeding materials generated through mutation breeding. Overall. having excellent quality fruit.08 cm in 0. Protocols for molecular breeding are in p r o g r e s s to streamline the methodology of RAPD and PCR base techniques. There was a constant and gradual decrease in plant height with the increase in radiation dose. Two mutant lines AEL 15/30 and AEL 23/40 (1648 kg/ha) were found promising. nodal length and stem diameter has been recorded. Its pathogenicity was proved and taxonomy of the fungus was studied. Chrysoperla carnea were hatched when the egg cards were attached within the top 15 cm plant canopy. Studies indicated the significance of the sunlight for successful application of bio-control technology in the field. Safeda were irradiated at 0. which was comparable with the insecticides treatments. some early lines endowed with high grain yield were selected. and the parasitoids was also less than insecticide treatments. Proposal of the candidate variety AEC86-347 (NIA2004) has been submitted to Technical Sub-Committee for the Approval of Varieties and Techniques for its approval. direct and reciprocal crosses between high yielding cultivars and exotic male sterile lines were also attempted to transfer male sterility trait. After thorough testing and confirmation of stability of seedless character upto mV5. The data on the seedling height.6 cm.4 kGy doses of gamma rays. after irradiating at different doses ranging from 20 Gy to 120 Gy indicated that irradiation reduced the immunity in the 4th instar larvae and greater number of the larvae was parasitized at 60 and 80Gy dose. a sugarcane variety developed at NIA. suppressed the bollworms infestation more effectively than separate t r e a t m e n t of p h e r o m o n e s or parasitoids at NIA. From two international nurseries. Tandojam gained popularity amongst the growers and has been grown on more than 5000 hectare in Sindh. Sugarcane borers Different stages of the larvae of Chilo infuscatellus exposed to parasitoids. Mango Some mango plants. Vegetables To study the mode of inheritance. A new disease Alternaria leaf blight by Alternaria alternata was also recorded. Moreover. The selected germplasm is under the process of multiplication and potential mango varieties will be offered to growers for commercial cultivation.3 kGy ENTOMOLOGY Cotton Pests Studies on the monitoring of A m e r i c a n . Guava About 4000 seeds of guava cv. it is ready for commercialization with the progressive kinnow-growers. About 1000 seedless Kinnow mutant-plants will be prepared and sold to the farmers during 2004.

Maize stem borer Trichogramma were released for the control of maize stem borer. Callosobruchus analis L. studied against the up surged population of pulse beetle. vine flour ('Maida'). Rice borers Among the three systemic granular insecticides tested for the control of rice stem borers. By using NIFATechniques. Stored Grain Pests Feeding and oviposition preference of red flour beetle Tribolium castaneum was high and development of larvae to adulls was rapid (24-28 days) in standard floj". Brassica aphids Twenty canola genotypes were evaluated for their variability in degree of susceptibility to the aphid attack and yield potential.About 250. The highest attraction percentage (17 %) of the traps was recorded in PMT baited with Acetic Acid + putrescine in 300 ml of water and two drops of Triton. Faisalabad Annual Report 2003-2004 27 . In umbrella guava orchard at Kohat. and there will be no need of insecticide in future if not infested from the neighboring fields. The parasites were released @ 20. while genotype TSA-1005/95 depicted the highest infestation and produced the least seeds. Maize stem borer larvae were collected from the maize fields in Peshawar and kept in the laboratory for observing the development of the larvae or emergence of larval parasitoids. fruit infestation decreased by 62% while percent damage (pupae/Kg of fruit) reduced by 60. Bio-control technology managed the infestation of borers effectively below economic threshold level. bran. a well established termite colony was excavated. NIFA-95 was found the most stable while Jubiha-1 was highly susceptible to the pulse beetle.000 pupae/acre in the form of cards. Results indicated that genotype.000 acres of sugarcane were treated successfully with biocontrol technology in Nawabshah. Application of water extract of neem seed reduced the fruit infestation and Pulse beetle Relative response of 7 chickpea genotypes (Cicer arietinum) was Mass Rearing Laboratory of beneficial Insects at NIAR. respectively. In Haripur. Trichogramma application resulted in a 69% decrease in the damage caused by maize stem borer. fruit damage by 61% and 41%. Studies on the quantification of aphids' population and yield showed that the genotype Agati Sarson (P) received the least aphids attack and produced the highest yield. An overall reduction of 44% was observed in the fly population due to all the IPM components employed in guava orchard. The genotype W-97-0. termites and sugarcane shoot borer as major pests of the crop.. The batch of maize stem borer larvae completing development in the first two weeks of Oct. where IPM practices like MAT. IPM studies on fruit fly control in guava orchards showed that during July . neem oil application caused 90% reduction in fruit infestation and 65% reduction in fruit damage. Diagnostic survey of the sugarcane crop at Tarnab. Mirpurkhas and H y d e r a b a d d i s t r i c t s w i t h coordination ofthe sugar mills during 2003-2004 season. Integration of Male annihilation technique (MAT). BAT and bio-pesticide were applied.75/11 harboured the least aphid attack and produced highestyield. and granular Fruit flies The Tandojam transparent plastic trap baited with protein hyrolysate and diammonium phosphate in a ratio of 3:7. 2003 indicated that all the larvae were parasitized and an average of ten cocoons of larval parasitoids were collected per parasitized larva. Cartap 4G proved the best toxicant on the basis of least borers infestation and maximum grain yield followed by the other two insecticides monome hypo and carbofuran. Noshehroferoz. Use of protein hydrolysate decreased the fruit infestation by 58% and fruit damage by 1%. attracted almost equal number of males and females of peach fruit flies.Dec 2003. Badin. respectively.4% compared with control. Bait application techniques (BAT) and cultural practices proved more effective than the separate treatments in most cases. Charsadda showed Gurdaspur borer and bugs in the ratoon crop and cut worms.

B. During early wilt incidence 198 entries were highly resistant. 100 resistant. Khan except Paharpur area. Basmati-370 and NIAB-IRRI-9.During this year 416 chickpea entries were received from NARC.juncea lines. napus. where it was inoculated artificially. ciceris.4mM) 28 Annual Report 2003-2004 . were characterized for resistance against them. more than 800 genotypes were planted for w h e a t rust monitoring. It was found only in the experimental fields of NARC.3. The preference of Rhyzopertha dominica adults was high for brown and milled rice. and in some adjacent fields where it might have developed as secondary infection through natural dispersal. 27% of the entries displayed resistant response to Alternaria blight while behavior of the 65% of the material remained between moderately resistant to moderately susceptible. while larvae developed to adults in brown rice only. Leaf cutter (Grass hopper) and leaf folder were found at damaging level in nearly all the places visited. Khan and Chakwal. AARI and NIAB for screening against Fusarium wilt. B. Islamabad. Some of the pest insects were collected for further studies.I. But at the end of March on the basis of total wilt incidence only three entries were found resistant. preference for wheat (dry and tempered) was low. Rangpur and Adhi Kot due to drought and it was good in irrigated belt of Thai and D. epidemiology and genetics at NIFA. carinata and B. Khan. Chakwal and NARC.I. D. opposition or hiding. Fifty new isolates of Xanthomonas oryzae oryzae.2. Due to high environmental temperature. 0. Different concentrations of salicylic acid gave 72-78 % protection to Chickpea against blight disease. 48 were susceptible and 363 were highly susceptible. campestris Chickpea pathology A survey of chickpea for prevalence of diseases was conducted in Thai. PLANT PATHOLOGY Wheat and oilseed pathology A wide range of wheat/oilseed brassica germoplasm was evaluated for disease/resistance assessment. Under collaborative work with Crop Diseases Research Program of NARC. White rust and Alternaria blight were the two main oilseed Brassica diseases observed during the season and a gene pool of 190 genotypes belonging to Brassica juncea.I. Induced Systemic Resistance (ISR) Induction of systemic resistänce in Chickpea against Ascochyta rabiei was checked in growth room. Crop was poor in barani area like Kot Shakir. However. hence survival was reduced and development was delayed upto 70 days. Highest prevalence of white rust was recorded in B.sp. No indigenous Pakistani variety out of 100 collected from PARC was found resistant against BLB caused by Xanthomonas oryzae oryzae. Response of 138 genotypes to white rust was immune while the remaining entries were found moderately resistant to highly susceptible. Among varieties. Islamabad. This indicates that material screened during last 2-3 years does not have strong basis of resistance against Fusarium oxysporum f. Tribolium castaneum beetles preferred paddy to brown and milled rice for feeding. Blight was not found throughout Thai. were isolated from local sites and purified from the diseased material collected during survey. 0. Lower and middle concentrations of Bion (0. IR-6 suffered lowest insects d a m a g e f o l l o w e d by DM-25.4mM) showed better protection (87-89%) as compared to highest concentration (0. D. 53 tolerant. Rice pathology Termite Queen detected by NIFA Entomologists starch ('Suji'). Survey of rice growing areas of Northern Punjab in October 2003 showed the presence of Bacterial Blight (BLB) caused by Xanthomonas oryzae oryzae in all northern districts. the causal agent of BLB. Occurrence of Alternaria blight was recorded in all the four groups. Goharwala. 59 lines selected as resistant during last year (20022003) showed susceptibility. Damage due to stem borer was also observed at many places but below 5%. 41 susceptible and 21 highly susceptible. Incidence of crop damage by pest insects was also observed during survey. two tolerant..

-0. increased the germination percentage of sorghum. out of sixty. The studies were found quite helpful to confirm agronomical results and proved that Sarsabz. Faisalabad. For screening of wheat genotypes under NaCI salinity stress. mass plantation on 1800 acres of saltaffected land. Sargodha. was appreciated by public on account of medicinal value ofthe barley grain. SD-4085 and SD766). Chilling alleviated the adverse effect of salt stress. Among these lines.25. germination and growth performance of 33 sunflower varieties was studied under different levels of salinity at seedling stage.75 & -1. It was observed that presoaking seed treatment with Gibberellic acid (GA) and Auxins (IAA and IBA) when used at 10 s to 10"6 M concentration had a pronounced effect on seed germination and seedling growth. The studies on brackish aquaculture technology showed benefit to cost ratio of 1:1. PaccaAnna.5. enzymatic activities and chlorophyll contents. These studies have revealed that germination was less affected upto 50 mM NaCI salinity. it was observed that seed priming with chilling and PEG. provided by the Fodder Research Institute. Phytohormones were used to reduce the adverse effects of salinity on wheat (Marvi. For the identification of sunflower varieties suited for cultivation on saline lands. the effect Income-generation The newly started income-generating activities include Eucalyptus oil and wood-shaving 'Buradah' production from leaf and wood of Eucalyptus tree.0 MPa). the field screening trials for selection of salt tolerant germplasm under saline conditions continued with safflower.42. It was also observed that glycinebetaine application enhanced drought-tolerance potential in sunflower and increased crop yield upto 10%. 13 medium sensitive and 2 sensitive genotypes. and establishment of large Host Rearing Laboratory for the parasites at NIA 29 Annual Report 2003-2004 . showed that Sargodha-late and Synthetic-82 varieties of berseem. new income generation activity comprising barleybased bakery products. At BSRS-II. and a package for lowcost brackish aquaculture technology was developed. SAFPD project Progress under the Saline Agriculture Farmers Participatory Development Project (SAFPDP) included installation of 12 tubewells.1 and 1:1. rice (Shua-92 and Sarshar) varieties evolved at NIA. was particularly positive under saline conditions. respectively. Under plant physiological studies. ten promising lines were studied with reference to biochemical response to proline accumulation. DS-11 and V-8319were comparatively salinity tolerant (8-12 dS/m). and Cargill variety of sroghum were the best salt-tolerant varieties at BSRS-I. Lahore. The economic analysis of the two activities showed benefit to cost ratio of 1:2. Moomal. Tandojam.(54%). PLANT PHYSIOLOGY AND SALINITY MANAGEMENT Salt Tolerance in Plants Screening for salt tolerance of 120 genotypes of rice has been carried out at different salinity levels under hydroponics system. 29 were tolerant.4. Chakwal-86. sorghum and chickpea varieties. 8 medium tolerant. Tandojam at five different agroecological zones of Sindh for evaluation of their yield performance under natural saline field conditions. -0. while at higher level (75 mM NaCI) germination was not only affected severely but was also delayed by one week. lining of 3 water courses.0 MPa. and reduced CI' and Na* while enhanced K+ and Caî+ accumulation in the shoots and roots of sorghum cultivars. Further testing of these 52 genotypes was done under net house conditions against terminal drought. Drought Resistance in Wheat One hundred-seventy wheat genotypes were tested under controlled conditions at different levels of stress treatment of PEG-6000 (-0. The other. It was observed that 52 genotypes had 60% germination at 1. On the basis of growth performance some promising lines have been identified. the results of field screening of berseem and sorghum varieties. in case of K 2 HP0 4 middle and highest concentrations (50 & 70 mM) had 75-83% protection and lower concentration (30mM) gave only 23% protection to seedlings against blight disease. eight salt tolerant lines were selected and grown alongwith locally known salt tolerant varieties of NIA. Whereas.

cotton tracts for biocontrol of pests on an area of 130 acres. Premixed and fertigation method increased potato yield by 14% and 17%. P and K content in leaves at boll opening stage of growth. AEM-96). use of ethylene (plant growth hormones) formulations has shown 15-25% increase in growth of leguminous and non-leguminous plants. Rhizobiology A research programme was initiated to study the feasibility of producing organic or bio-fertilizers (humâtes) from agricultural residues. growth.51 and 1. It was tested in a field experiment on wheat at NIAB farm. respectively. It can be concluded that cv. P-application Methods Studies continued to work out the suitability of fertigation technique for enhancing the efficiency of P utilization by different crops. efficacy of different irrigation systems for economizing water usage and enhancing crop productivity of wheat. Sohni and AEHM74 for intercropping with mungbean (cv. applied in combination with fertilizer-N. and thus saved considerable amount of irrigation water. receiving N in 3 splits. Tillage Practices A field experiment was conducted to identify the best timing of N fertilizer application to wheat sown by zerotillage and conventional-tillage practices. which may be one of the causes of poor seed germination/lower crop yield. The results revealed that 19 genotypes were efficient.54% and 22% water savings as compared to conventional irrigation system. They claimed that its spray on soil reduced evaporation from soil surface and suppressed transpiration from plants. improved seed germination. 2 medium efficient and 19 inefficient forZn. The studies on Zn efficiency of wheat genotypes continued and 40 genotypes were tested in chelatedbuffered nutrient solution employing 2. DAP and NP by producing 1. Bed & furrow and ridges systems resulted in . a new project on integrated nutrient management of young deciduous fruit orchards was started with already established young plants of plum and apricot. The data recorded on pruned products. Among four P fertilizers. farmers' method (broadcast) with pre-mixed (fertilizer + farm yard manure) and fertigation. whereas mycorrhizal treatment produced grain yield of 2867 kg/ha. indicating that phosphorus may be applied by premixed or fertigation method to obtain higher potato yield/economical crop production. dry matter and grain yields of wheat (lnqilab-91 ) in a salt-affected soil.28 tons ha"1. Studies were undertaken with the objectives to assess the relative EPS-Producing Bacteria An organic soil conditioner 'Eucompost' was prepared by inoculating the ground eucalyptus leaves waste with EPS-producing bacteria. Effect of "Agrocer-010" "Agrocer-010" is a 30% ethoxylated montan wax produced by M/s. triple superphosphate (TSP) proved to be the most effective P source for fertigation since it produced maximum grain yield of 1. The effect of phosphobacterial and mycorrhizal inoculation in wheat was studied. by using ordinary drill + planking. In case of zero-tillage maximum grain yield (2911 kg/ha) was obtained. and N. Different irrigation practices affected the water usage and crop harvests to a considerable extent. 10 and 40 pM Zn solution. field studies were conducted to evaluate the performance of four cotton cultivars viz. and the product is not only economical but significantly beneficial for plant growth also. however. whereas application of the same P-dose through DAP produced 8% less yield. Although it was tried to simulate the conditions of specially designed zerotillage sowing drill. could save one irrigation-water. was studied in a field experiment using two cotton genotypes (NIAB-111 and NIAB-98). NIAB-78. but the germination was 41% less for zero-tillage than conventional tillage.e. number of branches per plant. a byproduct of gelatin industry p r o d u c e d significantly higher seed cotton yield over control. Clariant Pakistan Limited. The greenhouse experiment showed that the use of'Eucompost' (having individual or mixture of the EPS-producing bacterial-isolates). 1. length of branches and nitrogen content of the leaves indicated that 75 percent of mineral nitrogen with 25 percent of farmyard manure was the best combination for both the orchards. The objective is to study the effect of mineral nitrogen applied alone and in different combinations with organic forms of nitrogen. The results showed that DCP. over the farmers' practice. respectively. The results showed that grain yield of 3016 kg/ha obtained by phosphobacterial inoculation was statistically at par with 75 kg P ha"1 (3164 kg/ha). Nodulation and N2 fixation significantly increased in Sesbania (a green manuring crop). SOIL SCIENCE AND PLANT NUTRITION Integrated Use of Fertilizers At NIFA. for comparison of P-application by different methods i. A field experiment on potato was performed at farmers' field. The effect of individual and integrated use of fertilizers on the production of seed cotton yield.60.The results of this study indicated that applying one Agrocer spray on soil. In continuation of the intercropping programme. Preliminary studies have already shown a good promise. Chandi. Likewise. the results need detailed cost/benefit study and conformation in other conditions/ soils. Annual Report 2003-2004 30 . AEHM-74 and mungbean AEM-96 were most compatible for intercropping system with lesser competitive effect on each other.75 tons ha"1 followed by SSP.

The samples were analyzed for microbiological. All the samples stored up to 5 months at ambient conditions were found fit for human consumption and will be studied for further storage. showed that differences among different fortified and control flours were nonsignificant. IAEA training course participants visiting a Kallar grass field at Shorkot 31 Annual Report 2003-2004 . The bitter gourd samples dried in electrical drier were in good condition after 1 year at room temperature proving the storage stability ofthe product. A pilot scale solar drier is being fabricated. Samples of 10 commercial feed brands were collected from the m a r k e t and a n a l y z e d . . which were purchased from local market.In preservation of fruits and vegetables by bottling. decrease remained within acceptable limits for 14 day storage. Work on five different Meals Ready to Eat (MRE) samples provided by Army Supply Corps Center continued. acceptability was also completed. this ièij£ÉL r v k M i J ï . however. The pH of brine as well as vegetables decreased during 6 months storage at room temperature indicating that peas could be stored at pH levels of up to 3. However. The present study suggested that a dose of 2.5 kGy in combination with low temperature (5°C) storage is effective for reduction of bioload and extension of shelf life of minimally processed fruits. A dose of 2 kGy was found enough to control fungal counts and coliforms in both the fruit samples. set for the acceptability study. ferrous sulfate as fortificant had numerical edge over others.5 without significant changes in most of physicochemical parameter during storage at ambient conditions. v. medium and high doses was conducted. Nutritional studies For "Iron Fortification of Wheat Flour" the work on second component of this project. Results of different parameters. the effect of different pH levels was studied to extend the storage stability of peas. The results after 3 months storage at room temperature showed linear response towards the irradiation treatment. ATCOP and PINSTECH) in the dose ranges of low. appearance and flavor of apples and melons decreased with increasing dose rate from 0 to 3 kGy. physicochemical and sensory quality. Feed consumption efficiency in irradiated feed group was slightly better than control group.e. Screening for Salt Tolerance in wheat (Glass House Study) FOOD SCIENCE Food/Feed Irradiation The response stability of irradiated polymethylocrylate (PMMA) strips (collaborative project between NIFA. Firmness. i. Post Harvest Technology The impact of gamma irradiation and low temperature storage on sensoric and microbiological quality attributes of minimally processed apples and melons was investigated.

To identify the begomovirus associated with the Mungbean yellow mosaic disease. During prepuberal and estrus phases progesterone remained at basal level.17%. In this project molecular diagnostic facilities for rinderpest detection were developed and used to monitor rinderpest in more than 12000 field samples from all over Pakistan.characterization of a birna virus disease of poultry known as infectious bursal disease (Gumboro) was possible for the first time in Pakistan. P a k i s t a n being d e c l a r e d rinderpest free for the first time. BIOTECHNOLOGY AND GENETIC ENGINEERING PLANT BIOTECHNOLOGY MolecularVirology Resistance breaking strain of cotton leaf curl virus from Burewala was analysed for its association with begomoviruses which showed that it is a recombinant molecule. Animal Health and Reproduction Hormonal aspects of growing 'Nili Ravi' buffalo were studied. Under the National Rinderpest Eradication Program 2003. In addition.06%. It remained at this level for 3-4 days and declined gradually to the basal level of 0. It was proposed that this recombinant molecule has evolved in a common host such as tomato that has recently mobilized into cotton. collected from Punjab Forestry Research Institute (PFRI). It started to increase gradually 3-4 days after estrus and reached the maximum level of 8-12 ng ml"1 within next 12 days of estrous cycle. A field experiment was conducted to study the variation in content and composition of essential oil in Trachyspermum ammi. a new vaccine has been launched in the market for the Hydropericardium Syndrome of poultry. A national level laboratory training course was organized that was attended by scientists.000 vials (80. as Vaccine Production During the period under report 8.27%) for 60 Kg N ha"1 application. efforts were made which resulted in Molecular Diagnosis of Animal Diseases At NIBGE RT-PCR based molecular diagnostic facility for morbillivirus diseases has been established to provide training and technology transfer of this molecular diagnosis. veterinarians and lab heads who are involved in animal disease diagnosis from all over the Pakistan. and 1000 vials (1 million doses) of NIAB-ND-vaccine amounting to 0. A significant variation in oil yield was found between tree-to-tree. Faisalabad. were hydro-distilled. and was above 1% only in 3 trees. Similarly. BIOLOGICAL CHEMISTRY Medicinal I Edible Oils Leaf samples of 14 trees of Eucalyptus camaldulensis. academics. whereas further increase in N dose (90 Kg N ha'1) significantly reduced the oil yield toi .9%) in control and 30 Kg N ha"1 samples. being maximum (2.000 vaccine doses) of NIABHS-vaccine amounting to 0.41 to 1. Through research using PCR the molecular Annual Report 2003-2004 32 .F. samples of infected mungbean were collected from various areas of N. Wheat Crop at maturity (Glass House Studv) The effective control of microbial load and non-existence of any negative nutritional repercussions of feed irradiation treatment.27 ng ml"1 within 5 days.55%. collected from NIAB. which played havoc with the poultry during this year. NIBGE participated in the national rinderpest eradication program for Pakistan through a multi-partied IAEA project.72 million rupees. Work is also underway for the preparation of vaccine against bird flu. Oil yield in different trees ranged between 0. leaf samples of 15 Eucalyptus globulus trees. ranging from 0. Faisalabad were hydrodistilled for extraction of essential oil. ('Ajwain') under different doses of nitrogen fertilizer.P and the Rinderpest eradication Rinderpest is the most fatal viral disease of cattle with potential to cause gigantic losses. This vaccine is to be sold in the market under the brand name of NIAB-Angara-Vaccine. makes this t r e a t m e n t an ideal p o t e n t i a l candidate for commercial application. Essential oil yield was almost similar (1.45 million rupees were prepared and sold in the local market.3 to 1.W.

In Rice Genomics group. 33 Annual Report 2003-2004 Transgenic sugarcane Bt-containing transgenic plants have been developed and analyzed for stable gene integration into the genome. Genetic Engineering Successful efforts have been made to isolate and characterize the Coat Protein (CP) gene of Potato Leaf Roll Virus (PLRV). protocols have been revised and new gene constructs are being developed with novel selection system. All the three lines tested exhibited high level of resistance against the locally isolated strains of Xoo. The role of the movement protein (MP) and nuclear shuttle protein (NSP) in the pathogenecity of Tomato leaf curl New Delhi virus (ToLCNDV) was studied which demonstrated that the ToLCNDV NSP is a symptom determinant as well as a target of host defense responses. NARC. known to confer resistance against the lepidopteran larvae. Genomics Cotton genotype NIBGE-2 performance has been compiled and it ranked 2nd in DGR trials. These lines are being evaluated at Vehari Research Station for Burewala virus resistance. Both types of plants have been developed and progeny transmission of transgene has been analyzed to confirm maternal inheritance. Chloroplast transgenic plants were developed to characterize the transgene expression and salt tolerance levels at heteron as well as homoplasmic states. Expressed toxins have been detected using immunodetection system. a biosafe way to control gene pollution. Analysis showed the presence of mungbean yellow mosaic India virus (IMYMV) in Pakistan. The sequence comparison revealed that it shared 94% nucleotide sequence identity with IMYMV confirming that bipartite begomovirus associated with the disease in Pakistan is a strain of IMYMV.Plastid genome for biosafety A chloroplasts-specific gene (ArDH) was isolated from yeast and expressed in a model plant tobacco. Islamabad along with 3 Basmati rice varieties. a phylogenetic relationship has been established between selected rice germplasm collected from PGRI. was synthesized based on polypeptide sequence through reverse engineering. Bacterial blight resistance (Xa21) gene was introduced into Super Basmati along with other varieties like Basmati-385. Several virus free cotton genotypes have been identified in year 2003 and are being tested again in this season. To overcome heteroplasmy and fertility problems in rice transgenic lines. One cotton genoytpe NIBGE-115 was found to be 100% virus free in virus infected area of cotton. Integration of the transgene was confirmed by Southern blotting in eight transgenic lines. A yeast gene named HSR1. was transformed into tobacco through Agrobacterium method and a number of transgenic tobacco plants were regenerated and confirmed through PCR. CP gene has been cloned in RNAi based vector to d e v e l o p PLRV r e s i s t a n c e in transgenic potato. Gen. It was successfully knocked out a PSII gene and homoplasmic lines have been developed. The transgenic plants showed high level of resistance against these insect larvae indicating that this gene is highly effective against these cotton pests. A novel procedure of gene knock out on copy correction mechanism has been developed. Several crosses have been made for drought tolerance and burewala virus resistance. Field studies of transgenic cotton Coker-312 lines previously showing r e s i s t a n c e to C L C u D u n d e r containment at Faisalabad (NIBGE) and Multan (CRS) showed stable resistance towards CLCuD. This is the first report of its own kind in the scientific field to develop null alleles of genes or open reading frames from chloroplast genome. The transgenic lines having HSR1 gene are being screened for salinity tolerance. ® Khalid Maqbool visiting various and facilities at NIBGE laboratories Punjab province. Governor Punjab Lt. biochemical analysis showed abolished photosynthetic activity in transgenic mutant plants. . A synthetic spider neurotoxin gene.

The HPLC analysis favor these results. Gibberellic acid production 34 . strains Ca-18. cDNA libraries from two of the developmental stages have been created and are being normalized to represent the complete number of genes in a given set of library. Twenty-two strains of Rhizobacteria from rhizosphere. Preliminary characterization has shown that all are fast growers.Microsatellite analysis has been done using different Basmati varieties and basmati breeding lines received from Kala Shah Kaku rice research station. PS-1. Biofertilizer application gave 30 % Zinc mobilizing rhizobacteria A microplot experiment was conducted to evaluate rice biofertilizer for zinc moblization. 309 and TAL-1827 were found to be bacteriocin positive. have been isolated and purified. The work on the identification of fiber development specific genes in cotton (Gossypium hirsutum) has been initiated. K-1.9 mg kg'1 of zinc content in grain and straw. Most of the isolates were placed in the genus Bacillus while the other isolates were assigned the genus Pseudomonas. ER-20. through partial 16S rRNA sequence analysis. These rhizobial strains are being characterized for IAA production. increase in crop yield with 75 % saving of N & P fertilizer. The capability of plant* growth promoting bacterial strains JCM1270. Fluorescence in situ hybridization Seven PGPR strains. using universal primers Y. 285. 4 gram variable. alkali producing and none of the isolates solubilized phosphate. All the isolates were found negative for phosphate solubilizing ability on Pikoviskia medium. Bacteriocin production Forty different Rhizobium strains have been screened for bacteriocin production by Defferd's antagonism method. 55 from endorhizosphere and 19 from rhizoplane. physiological and biochemical analysis of these isolates indicated that all isolates are gram negative. Plant Genome Resource Lab Plant genome resource lab is a newly developing lab at NIBGE. Induced systemic resistance Initially 22 rice associated PGPR strains from Tanzania and Pakistan were screened for their biocontrol potential against rice bacterial blight causing agent Xanthomonas oryzae. 96-51. PGPR strain 2. A project has been initiated to incorporate multiple bacterial blight resistance genes in Basmati lines. The grain and straw samples from rice harvest were processed for estimation of zinc content by atomic absorption. LC-21. antibiotic resistance and carbon source utilization. PS-4. The results obtained by using FISH were in accordance with the results from 16S rDNA sequence analysis. Molecular survey of rice germplasm and Basmati varieties has been done using the primers specific for bacterial blight resistance Xa4 gene. 281. 44 were gram positive and 48 gram negative strains. Ten strains LC-31. 236. Tissue Culture A novel system for commercialization of sugarcane elite varieties has been developed and trade marked as "Smart Greencane™ Seedlings". The phylogenetic analysis placed both the genera under different evolutionary branches.27 showed biocontrol activity against all of these pathogens.6 and 28. BIOFERTILIZERS PGPR association Cotton root associated isolates S-1 and S-8. isolated from cotton. Zinc mobilization was detected from Annual Report 2003-2004 Phytohormone production Seventeen Rhizobium and Bradyrhizobium strains were tested for IAA and GA produced. About 29 of the reported genes have been amplified and cloned in TA cloning vector and work on the cloning of more genes is in progress. These strains were further checked for their nodulation ability by plant infectivity assay. Analysis showed 23. respectively of PGPR inoculated plants. Ca-18 and 9651 was investigated for mobilization of soil insoluble or sparingly soluble zinc compounds in vitro. Rhizobia strains Five rhizobial strains were isolated from nodules of different guar (Cyamopsis tetragonoloba) plants grown in NIBGE field. Eight strains were positive for IAA production as identified by spottest. Re-inoculation experiments are being conducted in sterilized pouches to study host specificity as well as the effect of these five rhizobial inocula on the guar (Cyamopsis tetragonoloba) plants. Seven PGPR strains isolated from cotton were inoculated as single strain with 1/4th N&P using seed pelleting technique. were differentiated in to two 16S rDNA genotypes by using fluorescence in situ hybridization (FISH) with RNA targeted nucleic acid probes and confocal laser scanning microscopy (CLSM). earlier identified as Enterobacter and Azotobacter were later proved to be Bacillus fusiformis and Bradyrhizobium sp. ER-20 and JCM1270. rice fungal blight causing agent Fusarium moniliformae and rice s h e a t h blight causing a g e n t Rhizoctonia solani. Six developmental stages of developing fiber were selected for the construction of cDNA libraries. Morphological. A field experiment was conducted to study the beneficial effects of bacterial inoculants with cotton variety IR-FH-901. & Y2.

Moreover. Cloning of endo-glucanase gene The cDNA and genomic DNA of Flcarboxymethylcellulase (cmc1) of 35 Annual Report 2003-2004 . Certain regions including PLIEYYVESF and AAP were highly conserved among these enzymes and also with other enzymes from different genera. The deduced amino acid and DNA sequence of Xyn A was used to search for the homologous sequence in the SWISS-PROT database. this gene was transformed back in the parent using a constitutive promoter construct. The best mutant is being characterized for production of glucose oxidase. cerevisiae were also studied on 15% total sugars in molasses at different temperatures. rice and wheat crops in all the provinces of Pakistan. n/'/H gene was detected in the present study to further prove nitrogen fixing ability of Pseudomonas. Production of biochemicals Aspergillus niger strain was mutated using Y-ray in a s°Co irradiator.Aspergillus aculeatus were cloned in Escherichia coli. produced gibberellicacid. These sequences showed significant homology only to xylanase genes from genus Chaetomium. All kinetic parameters for substrate utilization and ethanol production for mutant strain of S. the invertase activity was determined for both parent and derepressed mutant strain of S. The maximum intracellular invertase activity of derepressed mutant and protein profile was significantly improved in the mutant derivative MD04 compared with its wild parent at 30 °C. Culture maintenance Culture collection. Biosorption based Pilot plant model for removal of heavy metals fabricated under Pak-Kazhak project was also tested by HPLC. niger were studied on different concentrations of total sugars. Alcohol/Methane production For improving the strain Saccharomyces cerevisaiae. Bacterial genes for plant growth Only a few strains with nitrogen fixing ability have been identified in Pseudomonas genus. 2003 to June. maize. followed by MNNG treatment. Using zymographic techniques four bands were obtained in the range of 20-50 KD. transformed fungus constitutively produced very high amount of CMCases on glucose and maltose. cerevisiae MD04 on different glucose and glucose + sucrose concentrations. Characterization of industrial enzymes After FPLC Gel filtration chromatography different xylanase peaks from C. Surprisingly. BioPower Nearly twentyeight thousand bags of BioPower were produced and distributed among the farmers from July. phosphate solubilizing and PGPR strains that have been locally isolated and characterized at NIBGE as well as obtained from other labs. and their nucleotide sequences were determined. which encodes the Fe-protein of the nitrogenase. thermophile were obtained. Hyper-expression of cmc1 gene in Aspergillus oryzae was obtained using plasmid pCMG14. None ofthe seventeen strains tested. preservation and maintenance of various (Brady) rhizobium. All kinetic parameters for substrate utilization and citric acid production for both the parent and mutant cultures of A. All nitrogep fixers carry nifH gene. 2004 for various legumes. Four bacterial strains isolated from cotton grown in Pakistani soils were submitted to DSMZ German culture collection to make them patent strains. Fourteen new rhizobial strains isolated from cowpea and common bean grown in Tanzanian soils have been deposited in BIRCEN culture collection ofthe Biofertilizerdivision. were carried out. cotton. The mutant strain was significantly improved over its wild parent with respect to both substrate consumption and product formation parameters. INDUSTRIAL BIOTECHNOLOGY Molecular Biology of Enzymes The C hectomiumn thermophile xylanase gene of 780 base pair has been sequenced and deposited in the Gene Bank.

0 at 37 °C. Indigenous Bacillus lichniformis RTS-1 (NIBGE) strain was used for alpha-amylase production. WASA. thirteen were detected as Salmonella typhi. Cloned product was confirmed in pBARMTE I. Success was achieved in purification up to the stage of obtaining a single protein band bySDS-PAGE. The maximum enzyme production was achieved 2700 SKB units with 10% corn steep liquor after 38 h of fermentation with pH 7. purification and characterization of a hemolysin from a uropathogenic Escherichia coli strain were completed. Sandle dyestuff. Arrangements were made to establish a 300-ton coal heap for biodesulfurization. This fragment was cloned in pBARMTE I vector. Fossil Fuel Biotechnology Serological analyses wëre carried out to study the microbial profile ofthe 20ton coal heap set for biodesulfurization. Arzoo Textile Mills. Production of Tylosin Hyper-producing mutants MFU-5. twelve as Salmonella paratyphi A. MFU-12 and MFU-18 of Streptomyces fradiae NRRL 2707 were refreshed in seed medium and then in vegetative medium and colonies were grown on plates to confirm their purity.sequence was restricted by xho I and sma I enzymes. etc forthe analysis of various parameters including heavy metals and anions such as Cyanide and Flouride in samples which is a prerequisite for ISO-9000and 14000 certification. Xho1 and Xma1. Nizam Pur for Biodesulphurization Production of alpha-amylase Work started on alpha-amylase production with collaboration of Biochemical Engineering RRL team. Growth studies were carried out to calculate the doubling time (td) and specific growth rate (m) for these bacteria. The estimated production cost of alphaamylase with present status of all chemicals and utilities was Rs. and five as Salmonella strasourg. resulted in acquisition of strains with maximum tylosin production of 40 mg/L. Xba1 respectively. atAskari Cement. The whole cassette containing sense and antisence fragment with intron Annual Report 2003-2004 . twentysix isolates were selected for further studies. Biosynthesis and Biotransformation of Mevastatin HPLC analysis of fermentation broths from 40 local isolates revealed five strains to show mevastatin production. Acidithiobacillus ferrooxidans (HC-AF2). T h i r t y s t r a i n s of p a t h o g e n i c Salmonellae were studied. Then these fragments were cloned in plant RNAi vector in order to incorporate the intron sequence between these fragments. Professional scientific services were provided to various departments/ industries including WAPDA. Chenab Fabrics and Processing Mills. will be directly applicable to blood samples fordiagnostic purposes. Nizampur. Services to Other Organizations Coal heap set atAskari Cement Factory. Maximum production obtained was 20 mg/L from isolate K19. The work on multiplex PCR for diagnosis of children suffering from diarrhea caused by pathogenic Escherichia coli was completed. isolated from local environments. Acidithiobacillus thiooxidans (KC-AT2) and Sulfobacillus thermosulfidooxidans (MT13) were used in the initial inoculum for coal heap biodesulfurization. Quantitative ELISA results indicated that moderate thermophiles were dominant bacteria in the leach liquor while in heap both mesophiles and moderate thermophiles were detected. 36 Biosynthesis of Cephalosporin C (CPC) The sense and anti sense fragments of CAH b gene previously cloned in pGEMT and T/A cloning vector were cut with Nco1. Distt. Nowshehra. 15/liter. 300 rpm and 40 L/min of air flow. Extremophiles Different solid and liquid samples were collected from Khewra salt mine. It is ready for use for characterizing stock cultures and with further optimization. respectively. Crescent Greenwood. Production. Screening of further 20 strains. HEALTH BIOTECHNOLOGY Typhoid Work on development of multiplex PCR conditions for molecular i d e n t i f i c a t i o n of p a t h o g e n i c Salmonella species was completed. On the basis of bacterial morphology.

9% BOD reduction was achieved by combined biological system. (for chronic myeloid leukemia) was carried out. Prenatal diagnostic service to the affected families has also been provided underthis program. cloned and propagated in E. Three families screened under this program requested for prenatal diagnosis. ALT level and PCR positivity. respectively. expressed protein was readily detected by SDSPAGE and confirmed by commercial ELISA test. Detection of Chromosomal Abnormalities Patients referred to the diagnostic laboratory (60 samples) were found to have karyotypes for Down syndrome. BOD and TSS reduction were the parameters considered in analyzing the process efficiency of UASB reactor.Hepatitis B Samples from 255 HBV positive patients by ELISA or screening were c o l l e c t e d to i n v e s t i g a t e t h e epidemiological distribution of HBV genotypes and to clarify further the genotype related differences in the pathogenicity of HBV.75L/day. coli. For characterizing the mutations causing ^5-thalassemia in Pakistan. at Sea View Karachi was carried out using different oil degrading bacterial strains. An already-established differential PCR was employed to detect fusion genes in 16 ALL patients. Hepatitis C virus for 1143 samples. Efforts have been made to i s o l a t e . Gleevec-resistance in Chronic Myeloid Leukemia (CML) An Allele-Specific Oligonucleotide (ASO) PCR for detection of mutations conferring Gleevec resistance in CML patients has been optimised. and coagulation by alum and activated carbon treatment for paper and board mill wastewater. Protein Pharmaceuticals Hepatitis B surface antigen (HBsAg) mutant of hepatitis B virus (HBV) is one ofthe important factors that result in immune escape and cause failure of immunization. rapid and costeffective technique for carrier screening and prenatal diagnosis of ^-thalassemia.3% COD and 94. 102 more HCV positive samples were analyzed for this purpose showing HCV-3 in 82% cases followed by genotype-1 in 12% cases. blood samples are being collected from various regions. 37 Annual Report 2003-2004 Bio-remediation Bioremediation of oil polluted area due to accidental spillage from an oil tanker (Tasman spirit). jS-thalassemia ^-thalassemia is the most common genetic disorder in Pakistan with a carrier frequency of more than 5%.21) (q22. respectively. The prevalence of genotypes was assessed further as regards patients age.5-0.9%) samples were positive for HBV DNA tested with PCR. The product. Hepatitis C Studies to find relation between ALT level and RT-PCR test for diagnosing HCV infection continued and from the analysis of 500 samples. DNA fragment encoding for the surface antigen from hepatitis B Virus was amplified. COD. 50% of samples were found to harbor AML1ETO fusion gene t(8. 50-80% and 14%. HCV genotyping by RFLP analysis continued. Aerobic treatment of wastewater The biological treatment of textile wastewater was observed in an aerobic-biological reactor operating in fed-batch mode. 35% patients showed to have mutations. Samples of crude oil contaminated seawater and sand were analyzed for % oil contents and moisture level.q22) was not detected in any sample. ENVIRONMENT Wastewater Treatment by UASB reactor The anaerobic treatment of wastewater from a textile industry was studied using a 35L UASB reactor. Chromosomal instabilities in Acute Myeloid Leukemia (AML) RT-PCR was e s t a b l i s h e d for differential diagnosis of AML. it was found that more than 76% patients with positive HCV-RNA have high ALT level while 17% samples have normal ALT level.q22) . The ARMS-PCR proved to be a powerful.5-95. The resultant effluent could meetNEQS. BOD and TSS removal varied from 40-65%. IVS-l-5 (G-C) and FSC8/9(+G) are found to be two most common mutations causing jSthalassemia in Pakistan. Services to Public PCR/DNA based detection of Mycobacterium tuberculosis for 185 samples. BCR-ABL fusion gene was detected in 50% patients. Philadelphia chromosome and other syndromes. The results showed that the average gas production was 0. Total maximum 89. sand filtration. The COD removal efficiency was 23% and 76% when COD loading rate was 210 mg/l/h and 1866 mg/l/h. This technique is now being utilized to manage Gleevec resistance in CML patients in clinical centers with collaboration of teaching hospitals. while CBFB-MYH11 fusion gene inv(16) (p13.2-91. It was found that only two hundred and fourteen (83. The COD. c l o n e and characterize the HBsAg DNA fragment from the HBV infected patients from local population. Using this assay. Hepatitis B virus for 92 samples and bcr-abl translocation for 20 samples. During this period.

PAEC has established 13 centers (including INOR Abbottabad recently inaugurated in May. Radioimmunoassay techniques are used to detect small NUCLEAR MEDICINE Pakistan Atomic Energy Commission is engaged in the application of nuclear technology for the socioeconomic development of the country and in the wake of growing incidence State of the-art equipment available at PAEC medical centres for diagnosis & treatment 38 Annual Report 2003-2004 . malignant pleural and Operational effusion etc. and 25 % more in treated pots over and above their respective control. polycythemia. The contribution of the Commission through its integrated programme in the diagnosis of different kinds of cancers and other diseases and their treatment has received considerable recognition and acclaim in the public and health circles. Facilities are also available for the treatment of thyrotoxicosis. Genotoxic effects of pesticides Organophosphorus (OP) pesticides (chloropyriphos) and Pyrithroides pesticide (cyhalothrin) along with Nitrosoguanine (positive control) were tested for their ability to induce in vivo genotoxic effect in blood and five fish organs. %age of damaged cells revealed a clear dose dependent increase. A significant increase in %age of damage cells indicating DNA damage was observed at 24 h posttreatment with both pesticides in comparison to control. PAEC Cancer Hospitals have the capabilities to renovate. obtaining planer. It was found that municipal and industrial wastewater drains are contaminating the rivers and canals and increasing pollution and are having adverse effects on aquatic life of water channels.000 patients are receiving benefit from our cancer care facilities annually. 2004) so far. which are providing latest and state of the art facilities for the diagnosis and treatment of various diseases including cancer. Commercial Services 350 water samples received from WAPDA. thyroid caner.000 patients were diagnosed by the Nuclear Medicine Departments using short lived isotopes. the percent decrease was 41%. paper mills and drinking water companies through PIBS were analyzed. After twenty weeks. size physiological and functional status of the organs. SPECT and while-body images of the diseased organs using gamma cameras. 192. pallivative bone pain. PAEC also has large resource base and operational strategy facilitates for timely repair and maintenance. During 2003-2004.Biodegradation of crude oil in soil mixed with sawdust was also studied. 30%. Mammography Machine installed at KIRAN Karachi of deadly disease cancer. health related applications of nuclear technology are of paramount importance. textile industries. More than 327. modernize and upgrade sophisticated electromechanical equipments attheirown. These images provide information about the shape.

steroid harmones. Approximately 108 kanal land has been acquired for Gilgit Centre where boundary wall and internal roads have already been built and accessory buildings like Officers and Staff Hostels are in construction stages whereas construction of main building is in tendering process. Bannu. Linear accelerators. Gujranwala. During 2003-2004 treatment was provided to more than 135. H DR remote afterloading machines and Superficial appliance etc. abroad. Research work was continued on 16 IAEA sponsored and 37 other research projects in collaboration with different international and national journals. scientific visits and participation in the scientific meetings and conferences and thus keeping abreast with the day-to-day changes occurring at the horizon of medical field. Moreover. We also extend expert services to regional countries for the use of invivo and in-vitro nuclear techniques through IAEA technical cooperation programmes. evaluation and prognosis of diseases are also available. This provides them with frequent opportunities of training. which calls for not only adding many more such cancer hospitals but also with more elaborate and high-quality facilities.PAEC medical centres providing a wide range of treatment for cancer and other allied diseases quantities of homones and bodies. various medical establishments are high enough that many foreign students are studying here for their postgraduate qualification especially in nuclear medicine. Saidu Sharif and Nawabshah and are in different stages of development. Mammography and Ultrasound for diagnosis. Recently IAEA has granted a TC project worth US$ 280. pituitary. gonadotropins and various tumor markers. 39 Annual Report 2003-2004 . Besides Radiology. Deep & superficial Xray machines. Similarly 92 kanal land has been acquired from Department of Auqaf Upgradation and Expansion Millions of dollars are being spent by Pakistani patients for seeking treatment of cancer-related diseases Research Projects PAEC nuclear medical centers have the added advantage of international contacts with more developed countries through International Atomic Energy Agency (IAEA) and bilateral relations with friendly countries. the standards of education and training at our Clinical Laboratory at KIRAN Karachi. It facilitates the access of our doctors and scientists to the international institutes and research organizations.000 on quality control and quality assurance of radiotherapy machines. Five more nuclear medical centres are being established at Gilgit.000 patients using Cobalt-60 teletherapy units. anti- The assays routnely carried out at PAEC medical centres include thyroid.

They also teach MCPS. The negotiation for acquiring land in Saidu Sharif and Nawabshah is in progress. Fourteen students from different hospitals are receiving training for FCPS at INMOL. DMRD students and also provide guidance to M. NORI and KIRAN.Sc. ( N u c l e a r Medicine). M.Phil students in collaboration with post-graduate institutes. Publications PAEC Nuclear Medical Centres published 9 papers in national/ international journals. DMRT. Nuclear Medicine and Radiotherapy to the MBBS students ofthe attached Medical Colleges.Sc. Doctors of PAEC Nuclear Medical Institutes are regularly teaching 40 Annual Report 2003-2004 . FCPS. Health Physician regularly. Nuclear Medicine and M. Teaching and Training PAEC is training it's own manpower by a w a r d i n g M.Punjab for establishment of Nuclear Medical Centre at Gujranwala. 10 doctors attended different IAEA training courses/workshops/meeting and 42 doctors participated in international/ national seminars/conferences/ workshops during this period.Sc.

e. D. uranium and trace elements. radioactive anomalies. Exploration and Mining in Pakistan. Khar Kot and Lassan areas Darband Geological and radiometric checking was conducted in Tanol Formation and Salkhala Formation exposed in Khar Kot and Lassan areas. Nizam Pur Basin Geological reconnaissance and foot radiometric surveys were conducted. volcanic material and hematitic alteration. Radioactivity is mainly associated with carbonaceous matter. cations/anions. Attock Basin Geological and radiometric studies were carried out on an area of 50km2 in the northwest of Gandghar Range but no significant radioactivity was detected. TDS. Anomalous radioactivity has been found associated with various lithologies which are manifested along the contact of fine and medium grained sandstone facies in the M a r d a n Khel S y n c l i n e . T h e radioactivity ranges from 150-1500 c/s against 70 c/s background. Khan an independent exploration project has been established for the prospection of uranium i.NUCLEAR MINERALS REGIONAL RECONNAISSANCE AND PROSPECTING Pakistan is bestowed with unique geological settings conducive to the formation of a variety of mineral resources. As a result. Radioactivity of the order of 5002000 c/s is associated with limonitic alteration within the graphitic schists of Salkhala Formation. Khan. Radiometric counts range from 150-800 c/s against 60-65 c/s background. The Gurguri Syncline has three anomalous zones. evaluation and exploration activities remained in the following areas: with radiometric counts ranging from 150-1500 c/s against 60 c/s background. Landai anomaly was checked for its lateral extension where few zones indicated radiometric counts upto 2000 c/s in the boulders. Radiometric counts range from 150900 c/s against 65 c/s background over a strike length of 150m. Due to significant uranium deposits in D. Nuclear Mineral Sector of Pakistan Atomic Energy Commission is carrying out Uranium Prospection. The quartzites of Tanol Formation exhibit spots of low radioactivity ranging between 150-180 c/s against the background value of 110 c/s. while second on its northern limb near the Mianji Khel village and has three levels alongwith yellowish uranium mineralization at one level and radiometric counts (150-1500 c/s against 60 c/s background) and third on the western most plunging part of the Gurguri Syncline in Shinkai area. Over an area of 600 km2. Lahore and its Regional Exploration Offices in Peshawar. Paleocurrent studies were conducted along and across the Mianji Khel and Gurguri anomalous sandstones. 300 c/s). gas wells & uranium mineralization was also prepared. These are Banda Shinkai anomaly and Tit Kamar Algad anomaly. two anomalies were found at two different sandstone horizons in north eastern part ofthe Karak Anticline. Satellite data of Nizam Pur basin was interpreted and furtherfield work plan was prepared. Rustam and Mardan Radiometry and surface sampling were done in Ambela Granitic Complex at Landai and Badar Ziarat anomalies. over a strike length of 300m. The Banda Shinkai anomaly is located about 400m SE of Banda Shinkai at NGR-424876. Detailed Exploration of Uranium Project. ranging 150 -400 c/s against 120c/s background. Karachi and Quetta. Generally the radiometric counts were low. Tit Kamar Algad anomaly is located about 400m SE of Mandanna village in Tit Kamar Algad. An updated composite map of Gurguri Syncline covering 75km* area depicting geology. The main work is being executed through Atomic Energy Minerals Centre. G. The basin hosts sufficiently thick sand/clay alterations with low radioactivity (max. These are mainly associated with hard bands in the host sandstones. one at southern limb near Alwar Killi locality. 38-0/8. Radiometric checking was conducted over 97km2 area. In NWFP the main thrust of prospection. G. 182km2 area was covered through hydrogeochemical survey in Gurguri & Banda Lakhoni Synclines and 125 water samples were collected from various sources for determination of Eh. Radioactivity is associated with hard bands and sandstone. There is rare indication of syndepositional volcanism. where radioactivity ranges between 1503000 c/s against 80 c/s background. 41 Annual Report 2003-2004 . Kohat Plateau Ground radiometry was conducted in the Nagri Formation exposed in Gurguri & Mardan Khel Synclines. Radiometric checking was completed over 45km2 area. This work is spread overall provinces of Pakistan. A few traverses were also made in younger tourmaline granite exposures of Gharkala in the vicinity of Mansehra and covered 50km2 area. pH. Ground radiometry of Nagri formation was also done in western plunging part of Banda Lakhoni Syncline and culminated in north eastern part ofthe Karak Anticline. Hazara Granitic Complex (Mansehra to Darband) Investigations were carried out in a new environment of Hazara Granitic Complex from Lassan to Nawab area about 35km NW of Mansehra.

Salgar & Surchar Nalas. Shallow reconnaissance exploratory drilling was carried out in Tuftan area. fractured granite filled by mica. Sand section showed 130 to 160 cps radiometric counts whereas clay indicates 110 to 120 c/s. Only the Dalbandin Formation has indicated good radiometric signature near Tuftan town. 350km2 area was scanned in the vicinities of Tuftan and Saindak. Preliminary Exploratory Drilling was carried out upto 450m in depth at three different locations to know the subsurface information.P. EXPLORATION AND EVALUATION N. sand and clay of recent sediments. Badwan and Osakai areas. radiometric.8. Chakdara area. SIND Shah Gadro-Kachhi area (Larkana) and Karonuk area (Sehwan) The reconnaissance survey was conducted over 70km strike length from Shah Gadro in the south to Kachhi in the north. Karonuk area (Sehwan) occupies structures of Bhadra Anticline and Naing Syncline. Lora and Ghora Gali Area Hazara Slates. track etch and gamma ray spectrometric surveys were conducted in Tuftan anomalous zone and adjoining area. Takwan and Manhre areas. Low radioactivity of the order of 150-200 c/s (on digging increased to 400 c/s) has been observed at three locations with a lateral extension of 30m. ROAC. It is mostly covered by silt. Shah Maqsood. pH ranges from 6. Baru Site. structural studies and detailed geological mapping were carried out in the Takwan Basin. eastern Salt Range. Structural studies were also done and it was found that the fractures trend NE and NW. extension of Paleocene unconformity and its relationship with underlying Formations. Radiometric counts range from 400-2400 c/s against the background of 150-170 c/s. District Dir Prospection survey was conducted f o r f l u o r i t e m i n e r a l in t h e surroundings of old fluorite mines at Chatpat locality and valleys of Chakdara granitic gneiss. Lithological. uranium contents range from 212ppb.97-8. Nali-Kuppar Basin. and TDS studies. Uranium mineralization (metatyuyamunite) was observed in one of the trench. Only spotty radioactivity upto 350 c/s against 50 c/s background was recorded. Radiometric zones ranging from 25-128 c/s were recorded against 10-20 c/s background. Detailed foot radiometric survey was conducted from Gaj (in the south) to Taki (in the north) over an area of 10km2 along the GS-I and GS-II of Manchar Formation (Wahi Pandi area). Radiometric counts range from 250-8000 c/s against 75 c/s background over 3-4 km strike length. Low-grade radioactivity ranges from 80-150 cps in the exposed strata of Manchar Formation. Juzakand Saindak Formations are exposed.W. L i m e s t o n e Sequence. Hydrogeochemical survey was carried out to the NW and SE of Gandghar Range in the Attock Basin. Rock samples at anomalous spots were collected and analyzed out of which one sample of Tuftan area indicated 627 ppm U308. Malakand Detailed evaluation of Malakand graphitic schist was completed as first phase. 5m excavation was carried out and 167 channel samples were collected. The radioactivity is associated with NE set of fractures filled by mica and continuation of foliated granites PUNJAB Eastern Salt Range Applied Research Group studied in detail the uranium occurrences in the Warcha sandstone. Hematization is also observed in the fractures while the excavated trenches indicate some solution movement.F. Laterite Beds and Murree Formation were radiometrically checked but no e n c o u r a g i n g radioactivity was recorded. while detailed sedimentological & structural studies were carried out along four geological cross sections in Saloi. Tanol F o r m a t i o n .GandgharRange Seventy km2 area was checked but no significant radioactivity could be recorded. Three trenches in Tuftan anomalous zone were excavated to understand the subsurface continuity of the anomaly. The Warcha sandstone has indirect contact with the laterite of 42 Annual Report 2003-2004 . Ara & Sidhandi areas. Momani-Gaj and Karonuk areas. Manki Formation. BALUCHISTAN Chagai Magmatic Belt Geological and radiometric investigations were conducted in the eastern block of Eruptive Zone. Fluorite mineralization occur as filling in shear zones associated with blue beryl in pegmatites and in the form of patches & pods in friable aplitic granite. Geological maps of Chakdara & surrounding areas were prepared and Grid control mapping was also carried out over an area of 500 x 500 Kirthar Range Reconnaissance radiometric survey was conducted along and across the Manchar Formation at Gaj. associated with limonitized fine grained sandstone covering an area of 50km2. Uranium mineralization is hosted by the fine grained. uranium contents. Major fluorite bearing zones were discovered at Jaba Banda. Hangu formation. 23 water samples were collected for pH. where Dalbandin. Chatpat.

for the preparation of an exploration map. Total Reasonably Assured Reserves (RAR) of uranium are 433. Weak radiometric zones have been recorded which vary in thickness from 0.42 to 5. to the north of the explored area for which EAR (estimated additional resource) of 1600 tons of U 3 0. Chamiari-Tarbela Evaluation studies of Tarbela graphitic metapelites were carried out. one in north of previously drilled development area and the other between 3000S to 3900S were prepared and proposed drill hole maps were completed. Photo mosaics studies of the area from Haleli Nala to Gaji Nai were carried out for verification of structural features. Shanawah Site-Bannu Basin Shoprang-Swat A detailed geological map was prepared depicting host tourmaline granite and its late derivatives. Uranium reserves estimation was carried out over one kilometer strike length of Shanawah deposit.5m thickness were encountered showing grades as much high as 600ppm of U. Three sedimentological sections were measured to a total line length of 11. Sori Nala and TakiDhoro localities.28 km2 at a scale of 1:500. NE-SW trending geological section about 11km length along the axis of the Nari Panoos syncline was also studied in detail. geological cross sections. Regional and detailed geological mapping of Nari Panoos and adjoining areas were done at 1:50.34 km were measured at a scale of 1:1000 & 1:2000. Lateral extension of Landai anomaly was also checked where a few zones indicated radiometric counts upto 2000 c/s in the boulders. Complex at Landai and Badar Ziarat anomalies. Exploratory drilling continued for a total of 800. radioactive spots. Two drilling plans. structural features and drilling fence lines for an area of 350 x 250 m was covered. upto a maximum of 2. cross bed data plotting of Gaji Kumb.49 metric tons over a strike length of 600m at level-1. Data was acquired during the field investigation for further interpretation. in which feldspars are moderately altered to kaolinite.464 km at Gaji Kumb.6km* area. 43 Annual Report 2003-2004 Rustam and Mardan Radiometry and surface sampling were done in Ambela Granitic . Nari Panoos Site-Kohat Plateau Evaluation studies were conducted at Nari Panoos anomalous horizon I. Out of this EAR. These cross sections were prepared across the four major anomalous sandstones (NP1-NP4) to establish the level of each anomaly in the stratigraphie column and to calculate drilling depth for future exploratory work. An area of 7. The ore is distributed at two main levels designated as 1&2 within Shanawah anomalous sandstone-1. The anomalous sandstone1 extend over a strike length of another 2. vertical fades map. compiled and interpreted. These samples indicated maximum chemical uranium of 222ppm with an average of 40ppm.5m thickness were encountered with maximum grade as 0. Sori and Taki as well as rose and vector diagrams and revised geological map of Gaji Kumb were finalized. Lithological and radiometric observations were recorded along these sections. Gamma and lithologs were prepared. Detailed geological cross sections were prepared across each anomalous zone. One geological cross section along fence line was completed covering 800m line length. The level-1 is expected to yield about 640 metric tons of U 3 0. upto a maximum of 9.55 meters (496. Detailed geological mapping was conducted over an area of 0. Shallow boreholes were drilled which amount to a total depth of 250 meters.5km. As much as nine lenses. Uranium resource at level-2 (30-35 meters below the level1 ) has 35-40 tons of U A only. SIND WahiPandi Dadu The exploration data was processed. Hydrogeochemical studies were proposed. Exploratory drilling amounting to 890m was done in graphitic metapelites and 400 core samples were collected at an interval of 0.3m.to the depth of 20m. amounting to 6584m with an additional reaming job of 144m. Drill hole log sections along strike & dip. Structural studies were carried out over 4. II & III.0„. for one kilometer strike length of the prospect.5km2 was mapped at a scale of 1:2000 while regional mapping was conducted at 1:25. The core samples show that the radioactivity is mostly associated with biotite rich part.000 & 1:50.30m for identification of chemical uranium/thorium and their equivalents.55m core) and as a result 25 different radiometric zones of various intensities and thicknesses were encountered. Detailed geological. Reconnaissance exploratory drilling remained in progress in the area. Four geological cross-sections with accumulative line length of 4.33% U 3 0„. Seven radiometric zones. Sori Rind. Shallow angle boreholes were drilled at Shoprang site which amount to a total depth of 280m. structural and radiometric studies were conducted along 15 numbers of geological profiles which were made at 50m interval.000 scales over an area of 120 km2. The samples indicated maximum chemical uranium upto 214ppm with an average of 52ppm U3Os.00m noncore & 304. The gamma log revealed maximum peak of 140 c/s against 80-100 c/s background. Detailed structural studies were conducted to understand the structural configuration of the anomalous block.000 and 1:2000 scales to locate future exploratory drill sites with respect to the prevailing structure and stratigraphie setting. may be added. about 30% is likely to occur in the oxidized zone (above water table).

track etch and gamma ray spectrometric surveys were done in Tuftan anomalous zone and adjoining areas. on permeability. Lithostructural elements and siesmotectonic stability of the area were checked. field groups for varied nature of elements/radicals. Sori and Taki. heavy minerals analysis. A reconnaissance survey was carried out in the vicinity of Ornach-Nal and southern Kirthar faults (Sind) to know the geological setup and neotectonic status ofthe area. thorium. ORE PROCESSING STUDIES In-Situ Leach Studies Studies on the core samples received from Shanawah uranium ore deposit pertaining to 244. Data was collected in uranium. Processing and printing of drill-hole data of Shanawah deposit was carried out for evaluation studies & In Situ Leach Mining Plan. MINING AND HEALTH PHYSICS Reports and available data was studied regarding exploitation of Sillai Patti ore deposit. REMOTE SENSING & COMPUTER APPLICATION Landsat TM data were processed and printed to enhance the lithology and structure of Dina (Sohawa). Kalat. porosity. Nagar Parkar. Relevant information were collected at site and further instructions were conveyed to drilling party. Chakdara & Peshawar. Larkana & Dadu Sind. Information about the mining machinery was collected frorri the relevant importers in the market. Chemistry laboratories carried out 16880 estimations on 5484 rock and water samples supplied by different Annual Report 2003-2004 Studies on Extraction of Rare Earths From Carbonatite Scout tests were performed on 500 kg Sillai Patti carbonatite rock for its physical upgradation. Exploratory deep drilling was carried out at Gaji Kumb and Sori sites. Software for mine ventilation network analysis was d e v e l o p e d for Shanawah ISL mining plan. Proposed bore holes for cross hole Seismic Survey at C-ll Site Chashma were investigated. XRD analysis and autoradiography.95m to 247. Jacobabad. GEOTECTONICS Landsat Image of Nagar Parkar area w a s interpreted for proposed repository site investigations. Sulaiman Range. A total of 2470m drilling was done including 818m of reaming job as well. Jogianosar & Mansehra. Resistivity survey was conducted in CPC colony area for designing cathodic corrosion protection of gas line network.Radioactivity ranging between 4000 to 6000 c/s and at places 10. potassium and total counts channels. Further studies on waxed core samples from different locations ofthe same site are in progress. The results revealed that the carbonatite can be physically upgraded from 0.95m depth.2% to 0. A proposal was prepared for drivage of two parallel experimental inclines of 100 hneters length each at Shanawah deposit site for monitoring of subsurface behavior of the strata for future underground mining plan. The study revealed that in this particular area only 26% leaching could be completed and according to mineralogical studies. Track Etch and G a m m a Ray Spectrometric Survey were carried out in Tuftan radiometric zone and adjoining areas.8%. ammoniums bi carbonate sodium bicarbonate and HjS0 4 . Rock samples of Marwat Range were prepared for fission track analysis. Distt. ROAC. associated with highly hematized/limonitized alteration. Preliminary high resolution seismic survey was conducted for the development of repository for subsurface assessment of salt bodies exposed in Manzalai anticline. Planning & dèsign work of ISL test ät Shanawah deposit site is continued.000 was recorded at the base of GS-II sandstone. The spectrometric survey alongwith different lines were also conducted to assess contribution of naturally occurring radioelements in depositional system. Halaksar. Alamreg & Tarbela.G. A seismic hazard map of Pakistan was produced. lixiviant suitability and oxidant consumption were performed by using different lixiviants. Khuzdar. Nizam Pur basin. D. 44 LABORATORIES Mineralogy laboratories carried out analysis of 1074 rock samples for petrography. The ASPF (Alpha Sensitive Plastic Film) related to track etch survey of Tuftan radiometric zone and adjoining areas have been dispatched to SSNTD laboratory PINSTECH for processing and counting. GEOPHYSICS ROAC and spectrometric survey data of Nari Panoos and Kohat Plateau was processed and interpreted. the low leaching of the sample may be attributed to the presence of high clay content in this particular portion of the strata. Kohat Plateau. Swat. The comparative studies on K-factor for gamma probes of vehicle mounted logging unit (MGX-II) and Robertson logging unit at Nangar Nai site were completed. Hydrogeochemical studies were conducted on water samples of boreholes at Gaji Kumb. such as sodium carbonate. Further experimentation was performed to observe the leaching behavior on the physically upgraded concentrate for the extraction of rate . Kohat. Lasbela & K h a r a n Distt. Khan & vicinity areas. B a l u c h i s t a n .

Soilcon rendered services to KCP-I. Preparation of maps showing earthquake epicenters distribution data on a scale of 1:10000000 and 12 number of graphs showing sea level fluctuation of the year 2003 for report on "Tsunami studies. It was revealed that the chromite ore was physically upgraded from 42% to 97% with overall recovery of 72. PAEC (MDP) and D. La Th) P0 4 . 78. = N. Geo-Consult Associates. C41.08% La203.05%. 74.402.93% Th). SERVICES TO SISTER ORGANIZATIONS Subsurface samples of one cubic feet size were collected from proposed Windmill site near KANUPP and analysed at AEMC Labs. La A .0%.33m depth including 37.28%. the rare earth elements and P2Os are present within permissible limits. DRE etc. C e A a n d N d A were leached giving the leaching efficiency as Physical Upgradation of Low-Grade Chromite Ore Two different batches.28%. Drilling amounted to 144.53% and Gd203 = 0.078% Th. one of 2125 kg and the other 1000 kg of low grade chromite ore received from HeroShah were processed for physical upgradation using induced magnetic separator and electrostatic separator. 1. 70. Shallow drilling was carried out upto 42. 11. 73. In this connection. the cleaning of rice husk/straw ash was treated with HCl to remove all the acid soluble impurities. Craftcone. the monazite ore was digested with sodium hydroxide to remove the phosphate.325/-. This thorium oxalate has Th0 2 = 56.0.Q. Nespak. Habib Rafique Ltd. KAPCO. Associates. During the production of thorium oxalate..01%. (Ce.82% (49. 0. Process Development for Production of High Purity Silicon The experimentation is being carried out to achieve the maximum recovery of high purity silicon crystals from rice husk / straw. The rare earths concentrate was separated from thorium concentrate and it contains 0. Y203. High Tech. H.004% U A . Fe Total = < 0. 2HjO has been prepared form monazite ore. 14. Dimen Associates. 74. La203 < 0. The bombreduction was carried out to reduce the Si0 2 to Si and the reduction was carried out successfully.04% and 74. One Longyear drill hole SA-I was drilled at Ravi Rayon area for geotechnical studies. 0.42% respectively.01%. S m A = 0. and 74% Nd203. which was received from Sonmiani beach near Karachi.. KANUPP Karachi" has been done. P r A .02% Si0 2 . CeOz = 0. 37.D. P A = 0.42%.01%.A. U. One borehole was drilled upto 450 m depth with a reaming job of 102 m. NMC-I. Detailed R&D proposal on the extraction of rare earth from carbonatite has been s u b m i t t e d for a p p r o v a l f r o m competent authority.5m depth for SPT & UDS tests at Kundian C-ll and Chashma C-llarea.20%. The silicon crystals were prepared and the efforts are being made to improve the purity ofthe crystals formed. GeoResearch. (Islamabad) worth Rs. Presson.earth elements. Process Development for Production of Thorium Metal from Monazite Ore The Thorium-oxalate Th(C 2 0 4 ) 2 . Berkeley school.7%. These results obtained are very encou-raging as the thorium oxalate produced is free from uranium and iron contents. 63% La 2 0„ 33% Y A .83 m reaming job as well. N d A < 0. Moreover.2%.18% Nd203 with traces of PAandFe 2 0 3 . OPF. 45 Annual Report 2003-2004 .M. Angora Textile Ltd.. This digested monazite concentrate was leached with HCl and thorium.27% Ce A . Newly purchased rig (Dando) Multi Tech-25 was commissioned and tested in the field environments at NMC-I Taunsa site. Descon. Using nitric acid the leaching efficiency on feed basis obtained was 58% C e A .

in the light of PAEC operating Preparation of Inspection Checklists As part of the DOS inspection programme. NCD and NMD were conducted jointly by DOS and PNRA officials. code of practices etc. RCPR reviews the PNRA documents including regulations. Faisalabad were also visited from perspective of medical emergencies. handling. Lahore. The regulatory inspections of PINSTECH facilities namely. experience and international practices. Removal System. PARR-2. Similarly regulatory inspection of C & F Branch Karachi was also jointly conducted by DOS and PNRA to inspect the receiving. DOS is setting up an appropriate professional environment with the technical tools necessary for carrying out Safety Analysis. checklists for Inspection of Radiation Protection Systems at KANUPP. storage. Participation of DOS Personnel in PNRA Regulatory Inspection Activities DOS personnel participated in the inspections of PAEC facilities conducted by PNRA. The RCPR comprises of six experts each from major activity of PAEC. Residual He?* Director DOS delivering a presentation on the occasion of Deputy Director General IAEA visit to PAEC HQs 46 Annual Report 2003-2004 . Review Committee for PNRA Regulations (RCPR) A technical committee called the "Review Committee for PNRA Regulations (RCPR)" has been constituted at the PAEC level and Directorate of Safety coordinates its activities.SAFETY Directorate of Safety (DOS) is a new directorate created within PAEC to promote safety culture and practices in all PAEC establishments in order to address the full spectrum of safety needs of PAEC. AEMC. DOS personnel keep a record of inspection findings and facilitate PNRA by expediting the execution of corrective actions suggested by PNRA. Karachi and PINUM. headed by a Member. DOS functions also include performing Internal Safety Audits of all PAEC establishments. Safety Assessment and Safety Reviews. DOS will encourage the management and employees in sponsoring and enhancing safety practices within their establishments. INMOL. Other experts can also be invited for detailed briefing if required. DOS is working as an intermediary and a focal point for interaction between Pakistan Nuclear Regulatory Authority (PNRA) and the relevant projects/establishments of PAEC. Auxiliary Feed Water System etc. In addition. PARR1. CENUM. These inspections covered inter alia operational safety. Radioactive Waste Management Systems at CHASNUPP and Safety Iniection Svstem. INSPECTION ACTIVITIES Regulatory Inspections of PAEC Projects DOS personnel performed preliminary inspection and audit of KANUPP Radiation Protection and Waste Management Systems. KIRAN. transport and delivery of radioactive material by DTP C & F Branch to various hospitals of PAEC and other consignees. of Chashma Nuclear Power Plant (CNPP) have been prepared during the period under report. Radioactive Waste Management Systems at KANUPP. regulatory guides. maintenance and surveillance activities.

Beijing Institute of Nuclear Engineering (BINE). The purpose was to test the integrated response capability of KNPC personnel with a realistic simulated incident. In order to get better understandings of the position of National Nuclear Safety Administration (NNSA). established in DOS. 2004. radiation protection as well as non-nuclear safety. chemistry and emergency planning & preparedness etc. said A comprehensive report indicating observations made during the above mentioned activities was also prepared. RFO-2 Activities at CHASNUPP-1 2nd Refueling Outage (RFO-2) activities at the Chashma Nuclear Power Plant (CHASNUPP-1) were conducted from April 10. PNRA. to review the regulatory activities of PNRA visited Pakistan from 30th November to 12th December. to carry out a comprehensive safety review of C-2 within PAEC before the review of PNRA. In this regard. importe + activities of the Refueling Outage. Richard Wessman of USNRC and Mr. Collaboration with China PAEC signed a contract with China for the purchase of Chashma Nuclear Power Plant (CNPP) Unit-2. DOS intends to establish similar Site Coordination Centres at KANUPP and other reactor sites in the future. as well as its Regional Co-ordination Centre located at the site participated as observers in the review areas of organization & administration. Site Coordination Centre at CHASNUPP DOS has set-up a Site Coordination Centre at CHASNUPP to continuously monitor the plant events/ activities on day to day basis and to provide quick feedback of the events occurring at the Plant. comprising of six members. This database may be helpful in identifying the particular HEME in case of accident/failure or any safety related problem of that equipment if occurred in relevant PAEC establishments.Emergency Response Management Drills DOS has established an Emergency Response Coordination (ERC) Centre at PAEC HQ to coordinate the activities of the local Emergency Preparedness Centres at different locations and provide guidance to them. 2004 as part of their radiological emergency plans. By establishing a Site Coordination Centre. as well as its Site Coordination Centre located at CNPP. DOS is in a position to keep a close watch on the activities/developments at the Plant especially those involving safety.2004 to conduct operational safety review of Chashma Nuclear Power Plant. technical support. participated as observers in the Database of Heavy Electrical and Mechanical Equipment (HEME) A database of heavy electrical and mechanical equipment (HEME) of twenty three (23) PAEC establishments using ORACLE software has been developed. 2004 to June 26. China Atomic Energy Authority (CAEA) and Institute of Nuclear and new Energy Technologies (INET). a five members delegation of DOS personnel. Lyn 47 Annual Report 2003-2004 . DOS personnel from Headquarters. That would be helpful for a very extensive safety review of C-2 as well as to develop a strong and sustainable nuclear safety infrastructure in PAEC. The drill was performed to fulfill one of the licensing requirements of National Regulatory Authority. Participation in the activities of International Regulatory Review Team (IRRT) An IAEA-IRRT expert mission. The database will further be helpful in investigating the root cause of the accident/failure of the particular equipment and taking preventive measures for similar equipment installed in otherestablishments. visited China from 28-03-2004 to 02-04-2004 and held meetings with various Chinese organizations including NNSA. DOS provided guidance to various PAEC establishments on important nuclear safety and radiation protection issues arising from time to time. coordinated the above mentioned Exercises/Drills at PAEC HQ. DOS Personnel from Headquarters. it is planned that DOS may enter into a bilateral agreement with National Nuclear Safety Administration (NNSA). maintenance. Sector and Station Emergency drills were conducted at KANUPP on 17th January. During this visit Mr.2004 to January 30. In this context. OSART Mission at CHASNUPP-1 IAEA Operational Safety Review Team (OSART) visited CHASNUPP1 at the invitation of Pakistan from January 12. DOS personnel witnessed the drill at KNPC site as observers. 2003. Advisory Services to PAEC Establishments The prime and foremost objective of the Directorate of Safety (DOS) is to enhance safety at PAEC establishments and to act as focal point for all matters related to nuclear safety. The Emergency Response Coordination (ERC) Centre. China and other concerned Chinese organizations regarding licensing of CNPP Unit-2 and to seek technical collaboration and training of DOS personnel in nuclear safety.

Recruitment. Qualification and Training of Personnel for Nuclear Power Plants. Joint Convention on the Safety of Spent Fuel Management and on the Safety of Radioactive Waste Management-INlFCIR/603/Rev. Agriculture and Education-Draft Safety Guide DS 67. reviewed provided. from 4-5 December. Research. Radiological Criteria for Radionuclides in Commodities. 2003 to observe the Inspection and Emergency Planning and Preparedness activities of PNRA. A report in this context was also submitted.7. RS-G-1. and comments were Guidance on Import and Export of Radioactive Sources. Organization and Implementation of a System for the Regulatory Control of Radiation Sources in Medicines. Industry.B e v i n g t o n of I A E A v i s i t e d CHASNUPP. Personnel from Directorate of Safety (DOS) participated/closely observed the IRRT mission activities during this period at CHASNUPP. 1 Code of Conduct on the Safety and Security of Radioactive Sources- Review of IAEA Draft Documents The following IAEA safety related documents have been critically 48 Annual Report 2003-2004 . IAEACODEOC/2004.

CTC conducted the M. Four weeks retraining program of all crews of MCR engineers and technicians was conducted on regular basis during reported period. IT programs for the sessions 2001-03. The 33rd batch (Session 2003-2004) with a total strength of 71 trainees joined KINPOE in December 2003. Nuclear Power Engineering Final result of the batch-9 (Session 2001-2002) was declared by NED University in September 2003. The Computer Literacy courses were successfully conducted for the children of PAEC Employees. IT (2002-04) started on October 07. First semester final examinations have concluded in June 2004 leaving the strength to 47. The 2nd batch of Master of Science in Information Technology.Sc. Ten (10) nominees from different PAEC departments joined the course. All the trainees joined their r e s p e c t i v e e s t a b l i s h m e n t s in November2003. 2003.Sc. 2003. Zero semester examinations concluded in February 2004.HUMAN RESOURCE DEVELOPMENT COMPUTER TRAINING CENTRE (CTC) The main activity at CTC is its training programs in computer system software and hardware to meet the requirement of skilled manpower in various PAEC projects/establishments. The 1st semester has been successfully completed. The semester will complete in September 2004. In the final semester (5th semester). First semester concluded in May 2004. Nineteen (19) fellows joined the course. Four months CHASNUPP systems course was also conducted. Five MCR engineers were examined and licensed by the Regulatory Authority for the position of Shift Engineer in the plant. Post Diploma Training Fifty-seven successful trainees ofthe batch-32 (Session 2002-2003) ofthe Post Diploma Training Program were relieved from KINPOE in November 2003. which is completely reserved for the project work. All the 32 successful graduates from this batch joined their respective places of posting in the last week of September 2003. Final semester classes are in progress. 2004. consisting of 37 students. Thirty-one (31) fellows joined the course out of which nineteen (19) fellows are studying in the 2nd semester. Seventy-one students appeared in the examination where as forty-nine students qualified. Post Graduate Training Thirty-three trainees of the batch-2 (Session 2002-2003) of Post Graduate Training Program joined KINPOE in the last week of August 2003. The 11th batch of Post Graduate Computer Orientation Course (PGCOC-11) started from 12th April. M. Currently sixteen (16) fellows are studying in the final (5th) semester. CHASNUPP CENTRE FOR NUCLEAR TRAINING (CHASCENT) P o s t g r a d u a t e t r a i n i n g of 36 CHASCENT fellows batch-2 and postdiploma training of 37 O&M technicians of batch 6 has been completed. IT Program The 1 st batch of Master of Science in Information Technology. Twentythree (23) nominees joined the course and twenty (20) successfully passed out on 6th October. 2002.Sc. 2004.Sc. Final semester examinations of the batch-10. which was completely reserved for projects. fifteen projects were completed by the fellows. Those students who had appeared in their matriculate examination were called for this program. Final examinations are scheduled in the second week of July 2004. (Session 2002-2003) was concluded in the second week of June 2004. Total class strength is twenty-four. Literacy Program for the Children of PAEC Employees A Computer Literacy Program was conducted for 59 children of PAEC employees from 10-05-2004 to 0406-2004. KANUPP INSTITUTE OF NUCLEAR POWER ENGINEERING (KINPOE) M. M. This course will complete on 11 th October. 2002-04 and 2003-05. IT (2001-03) passed out on 18-09-2003. out of which nine (9) are currently studying. M. M. Pre RFO lectures on lessons learned from RFO-1 and other topics were delivered to plant personnel involved in RFO-2 activities as well as a PGCOC Program The 10th batch of Post Graduate Computer Orientation Course (PGCOC-10) was conducted from 7th April. Fifteen students (10 Self-financed + 5 Fellows) successfully completed the course. 2004. 49 Annual Report 2003-2004 . 11th batch (Session 2003-2004) of 84 fellows joined KINPOE in the second week of December 2003.Sc. The 3rd batch of Master of Science in Information Technology. 2003 to 6th October. IT (2003-05) started on October 06. Participants of this course were nominees from different Universities and PAEC organizations. CTC also conducted its 10th regular Post Graduate Computer Orientation Course (PGCOC-10) in Computer Systems Software & Hardware and started the 11th batch of PGCOC.Sc.

Besides supporting SES programmes. An amount of Rs. The centre received Rs. High Precision Engineering (HPE). Fertilizer. Fauji PWI Training & Services PWI has conducted 12 different welding courses imparting training to 195 engineers. Seamless Tube Plant (STP). WAPDA. The Pakistan Welding Institute (PWI) is a professional institution having been established to maintain and promote standards of excellence in Welding Technology. Inspectest. PAC Karma.91 million was received from the clients. etc. PWI also offered its specialized services to M/s AWC. NCNDT provides training and certification programmes in various fields of NDT and also provides lnService-lnspection (ISI) and PreService-lnspection (PSI) to Chemical Plants Fertilizer and Oil Refineries.General Employees Training was imparted to foreign and local contractor's personnel. 0. An amount of Rs. Rousch Power Station. five (5) Training Courses were conducted in the field of Non-Destructive Testing techniques and 83 participants from local i n d u s t r i e s and v a r i o u s organizations of PAEC were trained. consultancy. Moody International. R&D. In-service Inspection on NonD e s t r u c t i v e E v a l u a t i o n of CHASNUPP during its Second Refueling Outage was dohe. SCIENTIFIC & ENGINEERING SERVICES The establishment of National Centre for Non-Destructive Testing (NCNDT) and The Pakistan Welding Institute (PWI) is another step forward by PAEC to pass on the benefits of its scientific and technical R&D efforts and to introduce the most advanced techniques to the industrial sector in Pakistan. PWI provides technical support through advice. CNPP. Almost all PAEC establishments. 50 Annual Report 2003-2004 . NDT Training & Inspection During the reporting period. Non-destructive Testing (NDT) techniques are being extensively used to improve and maintain the quality of manufactured goods as well as for the proper maintenance of industrial plants and equipment. 1. OGDCL. Tarbela Power Plant and PMD. Ittehad Engineering. PIA. strategic organisations and industries within government and private sector benefit from PWI. SNGPL. These services were mainly provided to M/s ARL. Life assessment of Plants and Third Party Inspection Activities were carried out regularly. 1.70 million.49 million as training fee for conducting these courses. KNPC.84 million was received as course fee from the participants. training and qualification. technicians & welders. The institution covers all areas of welding & joining technology and materials engineering for metals and non-metals alike. The centre also provided professional inspection & testing services to local industries worth Rs. PNS Mehran. Shifa Int'l Hospital. NDC. 1. Uch Power Station. KNFC.

Attock Refinery Limited (ARL). Petrochemical. Karachi Various Civil jobs at the Rest House i. manufacturing and at testing/ inspection stage. Construction of doctor's residence at NORI. 350 millions are under design. Pump Room and Electrical Sub-Station Room after receipt of administration approval and work will be awarded after opening offenders.G. workshops/labs to cater for training needs and also to provide services to national industries in the fields of NDT & welding. other PAEC Projects. DESIGN & MANUFACTURE OF EQUIPMENT/ COMPONENTS The Directorate manufacturée and supplied jobs worth Rs. Staff Hostel. Construction of misc. PC-II Form for Phase-VII (i. 183 millions to Heavy Mechanical Complex (HMC). Khan Exploration work remained in progress. N u c l e a r Equipment Workshop-I (NEW-I) at Islamabad. Faisalabad The work has been started and is in progress. A. Over Head Water Tank. civil works for rest house at Korangi. CNC machines. Face up-lifting at CENAR building at Quetta Work has been started and progressing well on schedule. 28. Islamabad The case is under process for getting administration approval of Competent Authority. Faislabad The renovation work of Phase-I & II was completed in all respects in March/June 2004 respectively and handed over to the end user.e. Oil & Gas Development Corporation (OGDC).e. Switzerland while jobs valuing Rs. Air Weapon C o m p l e x (AWC). Dr.03 million are in hand. 18. Pakistan Steel. CPC. The PC-II Form for Phase-V (July-2003 to June2008) has been forwarded to Planning & Development Division for approval. The remaining Construction work has been awarded and is in progress at the site. Heavy Industries Taxila (HIT). The construction of Boundary Wall was completed in March. PINSTECH. Lahore The Scheme remained operational on a very modest scale. is SCIENTIFIC & ENGINEERING SERVICES Scientific & Engineering Services Directorate (SES) was established with an explicit mandate to achieve self reliance in the manufacturing of specialized equipment for the programmes of PAEC and other strategic organizations.PROJECTS Renovation & miscellaneous work at NIAB. Development of on-farm facility at NIBGE. etc. SES has acquired the latest knowhow in Design Engineering & Equipment manufacturing and has established workshops having latest Welding Equipments. KANUPP. etc. WAPDA. DESTO. Chashma Nuclear Power Plant (CHASNUPP). The Directorate exported mechanical equipment worth Rs. PC-II for detailed exploration of Uranium resources in D. Simulator Building and 200 Bed Wards Block has been awarded to the Contractor and construction activities are in progress. Thus having acquired the capability of manufacturing high tech equipment and carrying out precision jobs for PAEC Projects and other Chemical. 2004-2009) has been forwarded to Planning & Development Division for approval. Septic Tank & Underground tank etc were completed and remaining work is in progress. The jobs of contract value Rs. Open tenders have been invited for the construction of Rest House. Fertilizer & Industrial Plants. Moreover. (KRL).e. Stress Relieving Furnaces. Construction of access control building at KANUPP. Karachi The building after completion in all respects has been handed over to the end user. S U P A R C O . extension of Nuclear Medicine & Combat Bunker. National Development Complex (NDC). SES was also given the responsibility of training in the field of NDT and Welding and setting up of specialised Construction of basic infrastructure at GINOR. Construction of Phase-ll at KIRAN. Karachi The construction Work of Phase-I I i. torily. Aluminium Casting. PC-II for nuclear mineral survey scheme of AEMC. Gilgit The Boundary Wall. 2004 and handed over to the under user as well.e. Karachi Electric Supply Corporation (KESC). Pakistan Navy. Nuclear Equipment Workshop-I I (NEW-I I) at Karachi and Nuclear Equipment Workshop-Ill (NEW-lll)[HMC-3] at Taxila. equipment manufacturing and in service inspection. Q. Watch Tower & Reception were completed satisfac- 51 Annual Report 2003-2004 . Destructive & Non-Destructive Testing Facilities and other equipment. The Scientific & Engineering Services Directorate (SES) has three e s t a b l i s h m e n t s i. Security Barracks. Khan Research Labs.334 million to CERN.

Similarly. 12J and 12L has been received and it is under review. Conference on NonDestructive Testing (NDT) The Second National Conference on NDT was held from 22-24 October. are under manufacturing. Electromagnetic Compatibility and Lightening Protection is in progress. etc. Heat Exchanger Shop and Light Machine Shop are under preparation. 5B. Manufacture of Equipment for Self-Adequacy 25-ton & 50-ton Welding Positioners and 200-ton Transport Bogie have been commissioned for HMC-3 own use whereas 50-ton Electrical Overhead Crane and 30-ton & 50-ton Roller Supporters. 12D.Quality Management Standards NEW-III (HMC-3) has attained ISO 9001 . NEW-III (HMC-3) All the construction work of Instrumentation & Control Labs. Islamabad. Appropriate System Documents for acquiring API Monogram Stamping of the products namely. been completed. A Scientific and Industrial Exhibition relating to NDT equipment has also held at -the same venue. 5D. 6A. A Federal Board has also registered HMC-3 as manufacturer of Boilers. Air Conditioning. 5L. Financial concurrence for the import of Flanging & Dishing Machine is in process. LCs for the import of Deep Hole Drilling Machine and Shot Blasting Equipment has been opened. Design & rectification of industrial electrical works for special equipment workshop and general workshop is in progress. HP/HT Test Loop is being enhanced and manufacture of Heater and Housing Assembly is nearing completion. Civil construction drawings of the Heavy Vessel Shop. has been completed.2000 certification for Design and Manufacturing of Process & Mechanical Equipment for Medium & Heavy Industries and Engineering Sectors. 2003 at Hotel Holiday Inn. Motorized Door has been installed. Ishfaq Ahmed. Correspondence with other organizations regarding different works is in progress. PHASE DEVELOPMENT OF NEW-3 (HMC-3) Construction Work NEW-I About 65% construction work of Phase-I and 50% construction work of Phase-ll of building for Pakistan W e l d i n g Institute have been completed. 12F. 5CT. Wall Panelling. the work on Electromagnetic Interference. The work on False Ceiling. 12DGU. HMC-3 has been authorized to use Symbols U and U2 of American Society of Mechanical Engineers in accordance with the applicable rules of the ASME Boiler and Pressure Vessels Codes. Trusses & Roof work have also been completed. The civil work of foundation and floor of Bay-2 Workshop has been completed. A runway for crane is in progress. Special Advisor to the Prime Minister honoured the inaugural session as chief guest. Ventilation and Humidity is in progress. etc. Design & execution of industrial electrical work is in progress. Pressure Vessels and Heat Exchangers. The participants include suppliers of various NDT equipment besides NCNpTand HMC-3. Installation of Equipment The 12-m Plate Heating Furnace. Dr. Roll-Way for 3 Roll Phase Plate Bending Machine and Vertical Milling Machine have been installed and tested. The construction work of Wood Workshop and Material Storage Shed is in progress and so far 25% work has been carried out. Columns. Thermal capacity of the NEW-I I The civil construction of Foundry Building and CNC Workshop has 52 Annual Report 2003-2004 .

addressing the 47th regular session of the IAEA General Conference developing countries. Mr. Chairman. 2005. World Association of Nuclear Operators (WANO)-Tokyo Centre. Parvez Butt. Vozo Miyazaki. 8-12 March. Chairman PAEC. Radiography Testing (RT-2). Biofertilizers for increasing sustainable crop production. Chairman. Aso Area on 1st April. Ultrasonic Testing (UT-2). Sustainable Energy Development and other N-technology related fields. Research Reactor Utilization. He also represented Pakistan as Governor from Pakistan in the Meetings of the IAEA Board of Governors held in Vienna (Austria) on 22 September. 2004. UK from 3-5 September. Germany from 12-14 October. 2004 and also undertook Technical Tour to Genkai Nuclear Power Station on 31 March. 2003. Non-Destructive Testing (NDT). 2004. Election was held at the Forty-seventh IAEA Annual General Conference. Chairman. Do Kyung Kim. Kundian and PAEC HQ. QA/QC Nuclear Analytical Techniques. Probabilistic Safety Assessment. Chairman. Islamabad on 19 January. 2003. Shahid Iqbal Siddiqui of KNPC. Review ofC&l Backfitting Equipment. Department of Material Advanced Institute of Science and Technology (KAIST).INTERNATIONAL RELATIONS Chairman's Visits Abroad Mr. PAEC participated in the Governing Board Meeting of (WANOTokyo Centre and WANO-Atlanta Centre) and Biennial General Meeting of World Association of Nuclear Operators held in Berlin. 2004 and Hatehobaru Geothermal Power Station & Mt. 2003. Pakistan Atomic Energy Commission attended as invited Speaker the World Nuclear Association (WNA) Annual Symposium held in London. Radiation Protection in Nuclear Power Plants. PAEC led Pakistan delegation to the forty-seventh IAEA Annual General Conference held in Vienna from 15 19 September. Pakistan Elected Member of the IAEA Board of Governors Pakistan has been elected as member of the Board of Governors of the International Atomic Energy Agency (IAEA) for a term of two years beginning from September. Republic of Korea visited PIEAS. Appointment of PAEC Official Abroad Mr. 2004 and 14-18 June. Foreign Delegations Prof. Review of KANUPP Desalination Plant and Management of Changes for Competitive Nuclear Power Performance. Japan on 30 March. Islamabad from 26-27 January. PAEC attended 2004 S p r i n g W A N O Tokyo C e n t r e Governing Board Meeting held at Fukuoka. On way back he also visited European Organization for Nuclear Research (CERN). 2003. 2004. Pakistan's election to this highest policy making body of the Agency is taken as the endorsement by the international community of the peaceful nuclear programme being pursued by Pakistan. Director. IAEA Experts in Pakistan Fifty nine IAEA Experts carried out assignments at different PAEC establishments under IAEA Regular Programme of technical co-operation in various fields including Mutation Breeding. Karachi carried out six months assignment as KANUPP Representative with CANDU Owners Group (COG) Office at Toronto. 2003 under Project Improving Safety Features of KANUPP. Switzerland from 15-16 October. Japan visited CHASNUPP. Medical Physics. 2004. 2003 to September. Canada from 6 December. 2003. 53 Annual Report 2003-2004 . Geneva. Pakistan has previously served on the IAEA Board for fourteen terms and with this extensive experience has consistently advocated the interest of the PAEC Scientists/Engineers as IAEA Experts Thirty three PAEC scientists / engineers carried out assignments abroad in various fields including Wheat Improvement. In-service Inspection.

Nigeria. Health. Hans Walischmiller. Mr. China visited Nuclear Institute of Agriculture (NIA). training at various PAEC establishments. a Meeting of National Representatives of RCA Member States and an International Conference were held during the reporting period. PSO. IAEA Research Contracts IAEA awarded a sum of US$ 109. Nuclear Medicine and Radiotherapy Support. Libya. PAEC Delegation Abroad A two member PAEC delegation comprising Dr. M. Z a h o o r A s l a m . Tandojam from 20-27 May. National I International Fora Ten National Meetings / Workshops / Seminars I Symposia. 2004 under the 16th S&T Protocol between Pakistan and China to prepare d e t a i l e d W o r k P l a n for t h e Collaborative Project on Biological Approaches for the Utilization of Salt Affected Soils/Bio-Restoration of Saline Waste Land and Development of Salt Tolerant Plants.712 which include experts visits. Dieter Novotny and Dr. an IAEA/RCA Regional Training Course. Malaysia. Mohsin Iqbal. Tomihiro Taniguchi. N I A B . Pakistan has participated in almost all the RCA projects. 2004 under 16th Protocol between Pakistan and China for Salty Soil Improving Technology by Using Biological Measures. Faisalabad visited Beijing. 2004. Prof. Non-Destructive Evaluation of Pressurized Components. equipment delivery. Department of Nuclear Safety and Security. Ing Ruben Avila Rodriguez. Institute of Soil and Fertilizer. fellowships/training and scientific visits. The amount represents the Agency's financial support for four new research contracts and renewing seventeen existing contracts. Technical Assistance by IAEA Twenty one operational IAEA Technical Cooperation projects broadly covering the fields of Biofertilizers for increasing sustainable crop production. Islamabad from 29 June to 6 July. 2004 and delivered lectures in the Symposium on Breast Cancer Screening. Radiation-Induced Mutations. Ms. Dr. Faisalabad and Dr. East Asia and the Pacific Section. Quality Assurance and Research Reactors Utilization. Sudan. Industry. Islamabad on 4 March. Isotope Hydrology. Dresden. Islamabad on 11 March.Mr. Chinese Academy of Agricultural Sciences. NIAB. Trevan Elizabeth Anne. Faculty of Engineering. National Autonomous University of Mexico visited PIEAS. Research Reactor Utilization. Director Breast Screen. Medical Physics. Director. Department of Technical Co-operation. People's Republic of China from 31 May to 13 June. Australia visited NORI. Naicheng Xu. Deputy Director General. Vienna (Austria) visited PAEC Headquarters. In-Service Inspection Facilities for Nuclear Power Plants. Nuclear Safety and Human Resource Development and Nuclear Technology Support were being carried out.500/-for undertaking research in various PAEC laboratories in different fields of nuclear science. Germany visited PINSTECH. PAEC on 30 January. Activities under RCA Pakistan has continuously played its effective role in the programme ofthe RCA and has gained substantially in the peaceful applications of nuclear t e c h n o l o g y in the a r e a s of Agriculture. IAEA. Gerhard Wargner (German Nationals) of M/S. IAEA visited PAEC establishments from 29 January to 3 February. Programme Management Officer. Department of Thermal Engineering. Beijing. Professor Yang Juncheng and Dr. Tanzania and Yemen availed fellowship 54 Annual Report 2003-2004 . Li Guihua. Foreign Trainees in Pakistan under IAEA Award Eight trainees from Kenya. Islamabad from 14-18 June. 2004 for having meeting with Counterparts of on-going Projects as well as the Counterparts of new Project Requests submitted to IAEA for consideration under IAEA Regular Programme of Technical Cooperation for 2005-2006. NDT Equipment Calibration. Mr. The technical assistance received during 2003 under IAEA Technical Co-operation Programme was US$ 929. 2004 in connection with MO-99 Production Facility. Lismore. 2004 to c o n d u c t M o d u l a r C o u r s e on Atmospheric Particle Dispersion Modelling. 2004 and also held meeting with Chairman.

596 1.637 2.719 146.508 90.122 502.000.829 208.414 232.292 57.933 127.460 22.49% +11.73% +0. Actual expenditure during the financial year under review along with its comparison with preceding year is given below: DEVELOPMENT EXPENDITURE Rupees (in million) Name of Sector Nuclear Power Mineral Development Agriculture Health Total Development Expenditure B.333 7.15% +515. 2003-2004.769 7.09% +24.279 27. Funds from generated receipts. CURRENT EXPENDITURE The current expenditure on different activities is as follows :Rupees (in million) Name of Sector Agriculture Health Physical Sciences Mineral Development Other Projects PAEC Hqrs Long Term Advances Collaboration with Universities Total Current Expenditure 2002-2003 235.036 305.688.91% +16.414.309 ± %-age +23.81% +14.759.585.236 354.91% -41.83% -2. • • • • Federal Government Provincial Governments Sale of Electricity Generated Receipts Funds received from Federal Government are utilised to meet the current as well as development expenditure of the Commission except Karachi Nuclear Power Complex (KNPC) and Chashma Nuclear Power Project (CNPP) which meet their expenditure from their own generated income.40% 1.446 ± %-age +20.531 - A.161 169.231 2003-2004 1.263 27.94% -1.047 55 Annual Report 2003-2004 .957 52.230 627. Provincial Governments and other sources aré mostly utilised for development activities.270 325. 2002-2003 8.90% -60.FINANCE Pakistan Atomic Energy Commission received funds from the following sources during the year.066 1.08% 2003-2004 291.392 165.86% +100% +19.

865 5.108 Million in 2002-2003 and Rs.310% and self-financing is to the tune of46.804% constitutes development activities while current expenditure represents 24.C.886%.246.721 * Including GST Rs.634 The revenue earned & receipt of KNPC and CNPP are as follows:Rupees (in million) KNPC.539 Million in 2003-2004 Rupees (in million) CNPP.790 816. Chashma Electricity Sales Revenue ** Receipts of Sales Revenue 2002-2003 3.637.42% Current Expenditure 1. 14. 91.608 2. 56 Annual Report 2003-2004 .650. 144.880 2003-2004 3. 128.484.90% CHASHMA NUCLEAR POWER PROJECT „ Rupees (in million) 2002-2003 2003-2004 2.212 50.044 ± %-age +30. Government of Pakistan has entrusted the Commission for establishment of 2nd Unit of Chashma Nuclear Power Project. SELF FINANCING KARACHI NUCLEAR POWER COMPLEX Rupees (in million) 2002-2003 Current Expenditure 847.644 2003-2004 772. Karachi Electricity Sales Revenue * Receipts of Sales Revenue 2002-2003 703.060 Million in 2003-2004 On successful operation of Chashma Nuclear Power Project (CNPP) at Chashma Mianwali since June. CONCLUSION An analysis of total expenditure of PAEC reveals that 28.392. 2000 by Pakistan Atomic Energy Commission (PAEC).904.822 2003-2004 111.812 ** Including GST Rs.170 ± %-age -8.799 Million in 2002-2003 and Rs.

Raza Akhlaq. Kamran Chughtai et al. 14. 17. M u h a m m a d Akram & A d a m . Tahir Siddiqui. Maint-PCR-03-01. " I n . 36.. Shahid Ali & Akhter K a m a l Q u r a s h i . S. M. "Steam Generator Condition E x a m i n a t i o n " . Mech. KANUPP-ISR-510-05. "The Role of Nuclear Power and Other Energy Options in Competitive Electricity Market of Pakistan". 10-11 February 2 0 0 4 . "Estimate of Burnup. KANUPP-ISR-330-35. Humayun Sultan. 10. Athar and M. "Chemical Cleaning of PHT Side of KANUPP Steam Generator. 19. CCD-PCR-03-01. "Forced outage due to high PHT close collection and high drop rate in CPH-TK1". October 2003. 32.. "Incident of Electrical Shock". Dubrovnik. Saleem Ayub. Rashidullah. Final Report under IAEA's Co-ordinated Research Programme on "Impact of Infrastructural Requirements on the Competitiveness of Nuclear Power". M. 11. Naeem Khan et al. "Rehabilitation of KANUPP Activity Monitoring System". 7. 41. 33. Shahid Raza. organized by C O M S A T S . M. 16. KANUPP-ISR-330-37. 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International Journal of Biology and Biotechnology. R. S. 2003. 369. The Nucleus 40(1-4) 375. Biol. P. (2004). et al. Agric Biol. EAS. et al. 350.Technol. On line J. Asian Journal of Plant Sciences. Wilczek)... Khatri.. Int. Ahn. Development of basmati rice varieties through the use of induced mutation and related techniques. Biol. 358. Laghari. et al. et al. and Extension. S. 1:49-57. Evaluation of various microorganisms for the production of bioactive metabolites. 6:366-369.. Microbial Biomass in Agricultural soils-determination. et al. Helicoverpa armigera (Hub. 1(1): 49-57. PJST 1(3): 1-12. 2003. 367. A. Upland cotton Varietal response to cotton leaf curl virus (CLCuV) Trop. Screening of chickpea (Cicer arietinum L. 86(1): 15-21. Efficacy of some insecticides against green peach aphid. Baloch. K h a l i d . 23: 258-259. et al. 374. & Bioltech.. 13(3): 173-182. Sei.S. J. 2003. roots and coleoptile of w h e a t (Triticum aestivum L) suitable for apoptotic and other molecular studies. Biol.E. M. Hamed. 352. Khalid P. A.. J. DNA marker Facilitated Analysis and Selection of Low Tiller Number in rice.. 351. 378. Khalid P.. J. J. Pak. 360. et al. 2003. Agri. J. M. I. 5:29-34. Mahboob.. et al. Khatri. Resistance in upland cotton to the whitefly transmitted cotton leaf curl virus disease. 2004. 2(1): 127-131. Fatty acid composition in muscle.J.An overview. 359. et al.. 349. 19(5): 257259. et al. M. Exploration of bioactive metabolities from saprophytic bacterial and fungal isolates of soil orgin.) genotypes. Resistance in u p l a n d c o t t o n to the w h i t e f l y transmitted Cotton leaf curl virus disease P E T R I A (Italian Society of Plant Pathology).. Seasonal effects and genotypic responses for grain yield in semi-dwarf wheat. 347.) and their impact on natural enemies in peach orchard.J. Res. Sial. et al.

1(2):181-186. et a l . 404. plants and animals during winter and summer seasons.) cultivars differing in yield potential. 413.. M. . 2 0 0 3 .. Shahnaz A. Sci. Int. Z. Pak. et al. Alleviation of salt stress in pearl millet [Pennisetum glaucum (L. 408. 399. J. A. Awan. M. B. et al. Water r e l a t i o n s a n d leaf gas e x c h a n g e prdperties in some elite canola (Brassica napus) lines under salt stress. Use of industrial effluent (CaCI2) for reclaiming salt-affected land and enhancing wheat yield. Nafees Altaf and M. Nadeem. Qasim. Int. Asif. et al. Study of photosynthetic efficiency of some guar (Cyamopsis tetragonoloba L.I. A s h r a f . 414. Naeem. latifolia Roxb. M. et al. total soluble sugars in seed. Towards a seedless cultivar of Kinnow mandarin. 2003.. et al. 398.. Iqbal. Y. IV. 397. Pak. Nafees Altaf.. 2003. 142:307-316. J. Y.. (2003). 405. Influence of different carbon and nitrogen sources on the production of glutamic acid by locally isolated bacteria. Flora 198:26-36. 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Physiological and biochemical changes in resistant and susceptible cotton varieties to leaf curl virus (CLCuV) cotton varieties at germination and early seedling stages: Changes in lipase. Pak. Growth and leaf gas exchange characteristic In Dalbergia sissoo Roxb and D..R.). Biologia Plantarum 46(4):629-632. 420.. Khan. starch. Zn2* status in soil.1. 35(5):771-777.Techn. J. et al... Comparative biochemical changes in resistant and susceptible cotton cultivars to ifeaf curl virus at germination and early seedling stage: é-amylase... Ashraf M „ A.. V. Wahed. Khan. Pak. 402. Mahmood. Towards a seedless cultivar of Kinnow mandarin. 2003. Biol. Akhter. Iqbal and Ehsan Ullah Khan. M. Biotech. Bot. Salt induced changes in two canola cultivars differing in salt tolerance. 2 0 0 4 . 2003.) R. 21 (4):63-66. Agronomie 23:227-234.) for yield and disease resistance. Z. J. et al. Translocation and recovery at maturity of the 15 N-labelled vegetativeN derived from foliar uptake of 1SNH. 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enhancing crop productivity. II. Effect of CaC 2 as a source of ethylene on nutrient uptake by wheat (Triticum aestivum L.). IntJ Biol Biotechnol 1,311-318. 423. Alam SM and S. Azam Shah. 2003. Effect of individual v e r s u s integrated use of phosphatic fertilizers on P-uptake and yield of maize and wheat. Pak. J. Soil Sei. 22:74-80. 424. Alam SM and S.A Shah. 2003. Effect of zinc and boron application on two rice genotypes. Pak. J. Soil Sei. 22(2): 6771. 425. Alam SM, et al., 2003. Effect of integrated use of industrial wastes and chemical fertilizers on phosphorus uptake and crop yields. Pak. J. Soil Sei. 22: 8186.

435. Azam F, Farooq S and Lodhi A (2003) Microbial biomass in agricultural soils determination, synthesis, dynamics and role in plant nutrition. Pak J Biol. Sei. 6,636-639. 436. Azam F, Lodhi A and Farooq S (2003) Response of flooded rice (Oryza sativa L.) to nitrogen application at two root-zone temperature regimes. Biol. Fertil. Soils. 38,21-25. 437. Azam F, Lodhi A and Sajjad MH (2003) Response of rice to nitrogenous fertilizer and G-irradiated sewage sludge. Pak J Sei. Ind. Res. 46,129-134. 438. Farooq S and Azam F (2003) Biotechnology Promethean science or obsession. Biotechnol 2,52-72. 439. Mahmood T, Kaiser WM (2003) Growth and solute composition of the salt-tolerant kallar grass [Leptochloa fusca (L.) Kunth] as affected by nitrogen source. Plant & Soil252:359-366. 440. Sajjad MH, Azam F and Lodhi A (2003) Nitrogen transformations in soil amended with different plant residues and their impact on growth of wheat. Pak J Biol. Sei. 6,805-813. 441. S h a h , K. H, et al., 2 0 0 3 . Performance of Fertigation Technique for P Usage Efficiency in Wheat. Asian J. Piant Sciences, 2(15-16): 1088-1091. 442. Shereen, A., et al., 2003. In vitro selection for salt tolerance among different strains of Bradirhizohium japonicum, Pak. J. Bot., 35(1): 89-92. 443. Soomro, A. M., 2003. A new high yielding mutant aromatic rice variety Khushboo-95. International Rice Research Notes. 28 (1): 32. 444. Shah.S. J. A., et al., (2003). Stability of rust resistance and yield potential of some ICARDA bread wheat lines in Pakistan. Pak.J.Sci.lnd.Res. 46(6): 443-446. 445. Raziuddin S., et al., Hormonal Effect on Callus Induction In Tomato. Sarhad J. Agric. Vol. 20, No.2,2004. 446. Iftikhar Ali and Mumtaz Ahmad, 2004. " In vitro mutagenesis in oilseed brassica"in Pak. J. Biotech. 1(1) 1-5. 447. R. Zamir, et al., 2003. In- vitro mutagenesis in guava (Psidium guajava L.). Pak. J. Bot. 35(5): 825-828. 448. Badshah, A., et al., (2004). Impact of genotype and location/environment on protein, gluten and moisture content of Pakistani wheat. Advances in Food Sciences. 26 (3). 449. Zamir R., et al., 2003. In Vitro mutagenesis in Guava (Psidium guajava L.). Pak. J. Bot., 35(5): 825-828,2003. 67 Annual Report 2003-2004

450. R. Zamir, et al., Effects of gamma rays on the rooting of guava shoot tips. Pak. J. Biotech. 1(1) 11-14 (2004). 451. Badshah, H., et al., 2003. Efficacy of Mercuric Chloride and Copper Sulaphate against termites (Heterotermes indicola) (Isoptera: Rhintermitidae) Sarhad J. Agric. Vol. 20, No.2, pp 275-278. 452. Khattak, S. U; A. Azhar and M. Shahid 2004. Effect of food diets on insect development and its sensitivity to gamma radiation. Pak. J. Agric. Res. 18 (1): 2530. 453. Javed, M. A., et al., 2003. Development of High Yielding Mutants of Brassica campestris L. cv. Toria Selection through Gamma Rays Irradiation. Asian J. Plant Sei., 2(2):192-195. 454. Khan, I. A., et al., 2003. Tissue culture studies in sugarcane. Pak. Sug. J. 18 (6): 37-40 455. Khan, l. A., et al., 2004. In-vitro studies in sugarcane. Pak. J. Biotech. 1: 6-10. 456. Khatri, A., et al., 2004. Mass production of banana (Musa spp.) through biotechnological techniques. Pak. J. Biotech. 1:15-17. 457. K h a n , I . A . , et a ! . , 2 0 0 4 . Performance of promising sugarcane cloneforyield and quality traits in different ecological zones of Sindh. Pak. J. Bot., 36 (1)83-92. 458. Khatri, A., et al., 2004. Study the performance of oilseed brassica in different water regime. Pak. J. Bot 36 (2): 351-357.

426. Alam SM, et al., 2003. Wheat yield and P-fertilizer efficiency as influenced by rate and integrated use of chemical and organic fertilizers. Pak. J. Soil Sei. 22(2): 72-76. 427. Altaf Hussain, et al., (2003) Application of Nuclear Techniques in Pesticides Research. Pak. J. Anal. Chem. 44 (2): 86-9. 428. Ashraf A, Mahmood T, Azam F (2003) Translocation and recovery of " N labelled N derived from foliar uptake of 1S NH3 by rice (Oryza sativa L.) cultivars. BiolFertilSoils, 38:257-260. 429. A s h r a f M, et a l , , (2004) C o m p a r a t i v e e f f e c t s of a p p l y i n g leguminous and non-leguminous green manures, and inorganic N on the biomass yield and nitrogen uptake in flooded rice (Oryza sativa L.) Biol Fertil Soils 40:147152. 430. Azam F and Farooq S (2003) An appraisal of methods for measuring symbiotic nitrogen fixation in legumes. Pak J Biol Sei 6,1631-1642. 431. Azam F and Farooq S (2003) Elevated COz and stress tolerance in crop plants with particular reference to agroclimatic conditions of Pakistan. Pak J Biol. Sei. 6,1096-1107. 432. Azam F and Farooq S (2003) Nitrification inhibition in soil and ecosystem functioning an overview. Pak J Biol. Sei. 6, 528-535. 433. Azam F and Farooq S (2003) Use of 2,4-dichlorophenoxyacetic acid (2,4-D) to facilitate bacterial infection of roots. Phytopath. 1,53-59. 434. Azam F and Müller C (2003) Effect of sodium chloride on denitrification in glucose amended soil treated with ammonium and nitrate nitrogen. J Plant NutrSoil Sei 166,594-600.

Conference Proceedings
459. Aurang Zeb, et al., Radiation D e c o n t a m i n a t i o n of Poultry f e e d Nutritional and biochemical Effects ofthe treatment. Proceedings of symposium on C o m m e r c i a l i z a t i o n of D e v e l o p e d Technologies. Challenges and opportunities. September, 21-22. 460. Aslam, M., Elahi, M.T. and Iqbal, N. 2003 (Published in 2004). Evaluation and c o n f i r m a t i o n of n o v e l g e n o t y p e s g e n e r a t e d under the project, "Development of improved germplasm of cotton through radiation and DNAmediated embryo transformation technique". Proc. Of 3rd and final RCM on "Industrial Crops" organized by the IAEA. IAEATECDOC Series No.1369:69-79. 461. Farooq S, et al., 2003. Electrolyte leaching and salinity susceptibility index as selection criteria for salt tolerant wheat

(Triticuum aestivum L.). Proceedings of 10th, int. Wheat Genetic. Symp. 2: 700702. 462. Hassan, M „ et al., 2004. Effect of NP on yield, quality and cotton disease. Proc. 4th International and 14th National Chemistry Conference. 463. Khattak, S. U; et al., 2001. Screening of different chickpea cultivars for resistance against pulse beetle, Callosobruchus maculatus (F.). Proc. 21st Pak. Cong. Zool. International. 21: 95-100. 464. Khattak, S. (J; et al., 2002. Screening of chickpea genotypes against gram pod borer (Helicoverpa armigera Hub.) using bioassay technique. Proc. 22nd Pak. Cong. Zool. 22:49-55. 465. Alamzeb, et al., 2002. Evaluation of pheroitione traps at different heights for management of chickpea pod borer (H. armigera Hub.). Proc. Pak. Cong. Zool. 22: 79-85. 466. Farid, A; et al., 2002. Selection of acaricides with least toxic effect on Sitotroga cerealella Oliv culture efficiency. Proc. 22nd Pak. Cong. Zool. 22:133-138. 467. Imtiaz, M., et al., 2004. "Zinc sorption in selected soils from Pakistan a n d E n g l a n d " . 10th I n t e r n a t i o n a l Congress of Soil Science held at SAU, Tando Jam from March 16-19,2004. 468. Jamali, K. D. and S. A. Ali 2003. " E x p l o i t a t i o n of s e m i - d w a r f w h e a t (Triticum aestivum L.) genotypes for high yield". Proc. 10th Int. Wheat Genetics Symp. 2: 727-729, 1-6 September, 2003, Pastern, Italy. 469. Memon, M. Y., et al., 2004. Effect of different N levels on various cottonlegume intercropping systems. 10lh international Congress of Soil Science held at SAU, Tando Jam from March 1619,2004. 470. M u k h t i a r A l i , et a l . , 2 0 0 3 . Phosphorus adsorption in representative soils of Peshawar Valley (NWFP). Pak. J. Sei. Ind. Res., 46(4): 288-294. 471. Shah, K. H „ et al., 2004. Efficiency of fertigation technique for economizing fertilizer P in berseem. 10th International Congress of Soil Science held at SAU, Tando Jam from March 16-19,2004. 472. Raja, A. R. and K. H. Shah, 2004. Evaluation of phosphobacterial and mycorrhizal in wheat. 10th International Congress of Soil Science held at SAU, Tando Jam from March 16-19,2004. 473. M. A. Haq, et al„ 2003. Induction of genetic variability for plant type and

disease resistance in chickpea, and its utilization in breeding. In: Sustainable Utilization of Plant Genetic Resources for Agricultural Production: Proceed, of Seminar, December 17-19 2002, NARC, Islamabad, pp. 28-37. 474. Khattak G. S. S., et al., 2003. Development of High Yielding and Disease Resistant Chickpea (c/cer anetinum L.) Mutants.//?.' Sustainable Utilization of Plant Genetic Resources for Agricultural Production: Proceeding of Seminar, 17-19 December 2002, NARC, Islamabad, Pakistan, p. 73-77.

inorganic electrolytes. In: Abstracts of the 10th Intl. Congress of Soil Science, organized by the Soil Sei. Soc. Pakistan, at Sindh Agric.l Univ., Tandojam,. p. 128 482. Ashraf, M., M.M. Iqbal and F. Hussain. 2003. Agrigels: the prospective soil conditioners for salt-affected soils. In: Abstracts ofthe 8th Natl. Meeting of Plant Scientists, Organized by Pak. Botanical Soc. at the University of Karachi, Karachi, Pakistan, p. 60 483. Zamir R., N. Ali, G. S. S.Khattak and T. Mohammad. 2003. Studies on the shelf life elongation in garlic (Allium sativum L.). In: 8th National Meeting of Plant Scientists Feb. 24-28, 2003, Department of Botany, University of Karachi, Pakistan. 484. S. J. A. Shah, et al., 2004. PostRelease Monitoring of Bakhtawar-92' and Screening of CIMMYT/ICARDA Bread Wheat Germplasm for Resistance to Yellow Rust (Puccinia Striiformis F. SP. Tritici) in Pakistan. In: Abstracts, Second Regional Yellow Rust Conference For Central & West Asia And North Africa, 2226 March 2004, Islamabad, Pakistan. Pp. 18.

Conference Presentations
475. Aslam, M. and Shahida Hasnain. 2004. Correlation and path coefficient studies of yield components in Upland cotton. G. hirsutum L. Proc. 2nd National Conf. Biol. Soc. Pakistan held at Govt. College University, Lahore, from 21-23 April, 2004. 476. Hina Syed, et al., Study for Fusarium wilt resistance in chickpea by using peroxide isozyme. Proc. 7th International Conference "Trends in Biochemistry and Molecular Biology", April 2-5,2003, Lahore, Pakistan. 477. M. Ahsanul Haq, et al., Induction of genetic variability for plant type and disease resistance in chickpea, and its utilization in breeding programme. Proc. Seminar on "Sustainable Utilization of Plant Genetic Resources for Agricultural Production" 17-19 Dec. 2002, NARC, Islamabad, Pakistan, pp.28-37. April 2003 478. Sadiq. M. S, et al., 2003. Induced genetic variability for economic traits in I A E A / R C A R e g i o n a l w o r k s h o p on " R e v i e w i n g R e s u l t s of M u t a n t Multilocation Trials" Oct 27-31, Manila Philippines. 479. S a d i q . M. S, et al., 2 0 0 3 . Diversified mungbean germplasm for seed yield potential in IAEA/ RCA Regional workshop on "Reviewing Results of Mutant Multilocation Trials" Oct 27-31, Manila Philippines. 480. T.M. Shah, et al., Utilization of wild chickpea germplasm for resistance to various stresses. Proc. Seminar on "Sustainable Utilization of Plant Genetic Resources for Agricultural Production" 17-19 Dec. 2002, NARC, Islamabad, Pakistan, pp.78-82. April 2003 481. Ashraf, M., F. Hussain and H. Shahida. 2004. Effect of bacterial exopolysaccharides on aggregate stability of a clay fraction obtained from a saltaffected soil following addition of

Pamphlets
485. Ali, Y., Z. Aslam and F. Hussain. 2003. "Nuclear Techniques Assisted Management of Salt Affected Waste Lands and Brackish Water in Pakistan" Recommendations for getting good yields of wheat from saline soils. Pamphlet in Urdu, 8 p. 486. Ali, Y., Z. Aslam and F. Hussain. 2004. "Nuclear Techniques Assisted Management of Salt Affected Waste Lands and Brackish Water in Pakistan". Recommendations for getting good yields of rice from saline soils.Pamphlet in Urdu, 7p. 487. Wahed, R.A., F. Hussain and M.M. I q b a l . 2 0 0 4 . D o n ' t e l i m i n a t e the Eucalyptus trees get profit froin its leaf biomass and wood. Urdu Pamphlet No.BSRS-ll/05, p.1.

Newsletters
488. Aslam, Z, et al., 2003. Sustainable use of ground water fot- plant production. Salinity & Environment Newsletter. Vol.1. (4), p. 4. 489. Zamir, R., G. S. S. Khattak and M. J. Qureshi. 2003. Cluster fruiting-a new mutant form in guava cultivar, Safeda in Pakistan. SAARC Agricultural Information Centre newsletter. 13(1): 910. (Bangladesh).

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Articles
490. Salihah, Z; A. Sattar; S.U.K. Khattak. 2004. Phasloon our Baghaat ki deemak ka marboot tareeqe Tadaraq. Urdu research bulletin pp 1-9. 491. Salihah, Z; A. Sattar; S.U.K. Khattak. 2004. Deemak kay gharoonday ka soragh bazaria NIFA-TERMAP. Urdu research bulletin, pp 1-4. 492. Khattak, S. U; et al., 2004. Fruit fly traps preparation and field applications. NIFA Res. Bull. (36): pp-3. 493. Khattak, S. U; et al., 2004. Fruit fly an important problem in fruits and vegetables and its control. NIFA Bulletin pp 5 (In Urdu). 494. Ahmadani, M.A., M.A. Arain and N.A. Nizamani. 2003. Crop weeds and their management. Sindh Ziraat 13(11): 67. 495. Ahmadani, M.A., M.A. Arain and N.A. Nizamani. 2003. Land preparation for wheat planting. Sindh Ziraat 13(11):67. 496. Ahmad, N., et al., 2003. Mewan je makh Ja Nuksan ain Bachaio. Monthly Zarae Dost (Sindhi), Karachi. Volume No. 5 August 2003. 497. Ahmad, N., et al., 2004. Kamand jai nuksankar jeetan ge hayatiati zaboto. Monthly Sindh Zarat, Karachi, June 2004. 498. Alam, S. M. 2003. Salinity and plant tolerance. Pakistan and Gulf Economist, (Karachi, Pakistan), June 30 July 6,XXII. No. 26, p. 32. 499. Alam, S. M. 2003. Water management. Pakistan and Gulf Economist, (Karachi, Pakistan), July 7-13, XXII. No. 27, pp. 41-42. 500. Alam, S. M. 2003. Role of woman in agriculture and social activities. Pakistan and Gulf Economist, (Karachi, Pakistan), July 14-20, XXII. No. 28, pp. 40-41. 501. Alam, S. M and M. H. Naqvi. 2003. Agricultural technology for crop growth. Pakistan and Gulf Economist, (Karachi, Pakistan), July 21-27, XXII. No. 29, pp. 27-29. 502. Alam, S.M. and M.H. Naqvi. 2003. Livestock vision in the country. Financial Post (Karachi, Pakistan). Wednesday, July23, p. 4. 503. Alam, S.M. 2003. Maximizing agriculture growth. Pakistan and Gulf Economist, (Karachi, Pakistan), August 3, XXII. No. 30, pp. 27-28. 504. Alam, S.M. 2003. Frontiers in crop productivity. Pakistan and Gulf Economist, (Karachi, Pakistan), August 4-10, XXII. No. 31, pp. 28-29.

505. Alam, S.M and M.A. Khan. 2003. Pineapple A delicious fruit. Financial Post (Karachi, Pakistan). Tuesday, August 5, p. 4. 506. Alam, S.M and M.A. Khan. 2003. The proteins Component of life. Financial Post (Karachi, Pakistan). Friday, August 8, p. 4. 507. Alam, S. M and M.H. Naqvi. 2003. Agriculture in SAARC countries. Pakistan and Gulf Economist, (Karachi, Pakistan), August 11-17, XXII. No. 32, pp. 30-33. 508. Alam, S. M.. 2003. Integrated plant nutrient management. Pakistan and Gulf Economist, (Karachi, Pakistan), August 18-24, XXII. No. 33, pp. 16-18. 509. Alam, S. M. 2003. Importance of water. Financial Post (Karachi, Pakistan). Monday, August 18, p. 4. 510. Alam, S. M. 2003. Worldwide rice production. Pakistan and Gulf Economist (Karachi, Pakistan), Sept. 1-7, XXII No. 35:19-29. 511. A l a m , S. M. 2 0 0 3 . G r o w t h prospects and constraints in agriculturePakistan and Gulf Economist, (Karachi, Pakistan), Sept. 8-14, Vol. XXII. No. 36: 16-19. 512. Alam, S. M., etal., 2003. Berseeman important fodder crop. Financial Post (Karachi, Pakistan). 16Sept.,X(123):4. 513. Alam, S. M. 2003. Salt affected soils. Financial Post (Karachi, Pakistan), 19Sept.,X(120):4. 514. Alam, S. M. 2003. Using hydroponic system in Thar desert. Financial Post (Karachi, Pakistan)., Sept. 26, p. 4. 515. Alam, S.M. 2003. Soil as supporting media for plant growth. Pakistan and Gulf Economist, (Karachi, Pakistan), Vol. XXII. No. 39:16-17. 516. Alam, S.M. and M.A. Khan. 2003. Growth regulator and agriculture. Financial Post (Karachi, Pakistan). X(147): 4. 517. Alam, S. M. 2003. Pakistan agriculture and farmers. Pakistan and Gulf Economist, (Karachi, Pakistan), Vol. XXII. No.40:37-40. 518. Alam, S. M. 2003. Global warming. Pakistan and Gulf Economist (Karachi, Pakistan)., October 20-26, XXII, No. 42: 41. 519. Alam, S. M. 2003. Food security t h r e a t e n e d by p o p u l a t i o n g r o w t h . Pakistan and Gulf Economist (Karachi, Pakistan)., October 27 - November 2, XXII, No.43: 37. 520. Alam, S.M. 2003. Mango The king of fruits. Financial Post (Karachi, Pakistan)., October28, p. 4.

521. Alam, S. M. 2003. Fighting salinity. Pakistan and Gulf Economist (Karachi, Pakistan)., November 3-7, XXII, No. 44: 15. 522. Alam, S.M. 2003. Neem A wonderful tree. Financial Post (Karachi, Pakistan)., Friday, November7, p. 4. 523. Alam, S.M. and M.H. Naqvi. 2003. Importance of potassium in plant growth. Daily Dawn (Karachi, Pakistan), November 10,2003, pp. IV. 524. Alam, S.M. 2003. Tree planting in desert areas of country. Pakistan and Gulf Economist (Karachi, Pakistan)., November 10-16, XXII, No. 45:35. 525. Alam, S.M. and M.H. Naqvi. 2003. Plant growth regulators. Daily Dawn (Karachi, Pakistan), November 17, 2003, pp. IV. 526. Alam, S.M. 2003. Citrus World's leading tree fruit crop. Financial Post (Karachi, Pakistan)., Wednesday 19, November5, p. 4. 527. Alam, S.M. 2003. Food production in the country. Financial Post (Karachi, Pakistan)., Monday, Novernber24, p. 4. 528. Alam, S.M. 2003. Food security and consumption patterns. Financial Post (Karachi, Pakistan)., Monday, November 24, p. 4. 529. Alam, S.M. 2003. Environmental pollution. Pakistan and Gulf Economist (Karachi, Pakistan)., November 24 December7, XXII, No. 47:38-39. 530. Alam, S.M. 2003. Weeds and their ill-effects on main crops. Daily Dawn (Karachi, Pakistan), December 1. 531. Alam, S.M, 2003. Radioisotopes. Pakistan and Gulf Economist (Karachi, Pakistan)., Dec. 8-15, XXII, No. 49:30. 532. Alam, S.M., M.A. Khan and S.M. Mujtaba. 2004. Declining of fertility of land in Sindh. Business Recorder, 1st April, p. 4. 533. Mujtaba, S.M. and S.M. Alam. 2003. Phases of rice growth. Business Recorder (Karachi, Pakistan), Sunday July6, p. 4. 534. Arain, M.A. and M.A. Sial, 2003. Wheat varieties of NIA Tando Jam. 2003. Sindh Z a r a a t l 3(10):5. 535. Jafri., K. A., J. Fazal Ali and N.A. Sheikh. 2003. New mungbean variety AEM-96: A helping tool in improving mungbean yield in Sindh province. Sindh Zaraat (Sindhi), 135:11. 536. Sial, M.A., M.A. Arain and N.A. Nizamani. 2003. The necessity of timely planting of wheat. Sindh Zaraat 13(12): 32-33.

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2003. Safeda in Pakistan. Parvez. H a f e e z . 2003. 39(3): 179-185. Shah. Khattak.c e l l o b i o s i d a s e s y n t h e s i s in Aspergillus niger. I.) through Induced Mutations and In Vitro Culture. Ashraf. Ali. S. A. A . A novel vector for e x p r e s s i o n / s e c r e t i o n of properly folded eukaryotic proteins: a comparative study on cytoplasmic and p e r i p l a s m i c e x p r e s s i o n of h u m a n epidermal growth factor in E. pp 5. (2003) "Toxicity testing of Chlorpyrifos in Fish". Report of the Second Research Coordination Meeting of FAO/iAEA Coordinated Research Project. Biochem. H. Khattak. Austria. Mansoor. Kinetics of i m p r o v e d p r o d u c t i v i t y and thermostability of a mutant-derived i n t r a c e l l u l a r . (2003). Rajoka.. 25:945-948. F. Parvez. 2 0 0 4 . Project Report for 2000-2003. and S. 544. et al..J. 560. Yasmin. SAARC Agricultural Information Centre newsletter. 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et al.. 574. Isolation. Farooq. G. A. Final meeting of CFC/ICAC 07 project held in ICBA. Gordon Research Conference on Water and Salt stress in Plants held at Hong Kong.E. Peshawar.A. (2003) Detection of irradiation treatment of meet samples using comet assay. 2 0 0 4 at N I B G E / COMSTECH. University of Sindh Jamshoro. International Workshop on Advanced Techniques in Biotechnology.. 2004.. International Animal Nutrition Conference. Rad. et al. Nterminal and C-terminai domains of truncated replicase gene of cotton leaf curl virus elicit resistance against the virus in transgenic tobacco plants.. (2004). M. Fsd.. et al. 2004. South Africa February 15-20. January 28-30.et al. Asad. Asad S. ICBA. S. Farooq. Khan Q M and Haq. Final meeting of CFC/ICAC 07 project. Pakistan. China. M. September 28-30. F. Mansoor.Challenges and opportunities" September21-22.K. et al.S. "Molecular based diagnostic of rinderpest virus by using Trizol". Shangrila Printers. (2003) "Application of Biotechnology for the T r e a t m e n t of I n d u s t r i a l e f f l u e n t s " September. DNA Damage induced by Cyhalothrin in Fresh Water Fish. 576. 578. Use of RNA interference for silencing of two begomovirus genes. et al. (2004) "Removal of Cadmium from aqueous s o l u t i o n w i t h the help of L a b e o rohitascales". Institute of Biochemistry and Biotechnology. 590. 4th National Conference of Plant Pathology. Lahore. A.. Final meeting of CFC/ICAC 07 project held in ICBA. 4th International Geminivirus Symposium / 2nd International ssDNA Virus Workshop: Cape Town. October 14-16. and Faiz. 575. ICBA.coli: study of b i o a c t i v i t y . et al. (2004). 71 Annual Report 2003-2004 ... A resistance breaking strain of CLCuV appears in Pakistan. et al. et al. and Nasim. M. Hafeez. May 1618.. Rawalpindi.1 7 J a n u a r y . ( 2 0 0 3 ) .. 597. Zahoor.. Final meeting of CFC/ICAC 07 project ICBA. M. M.. October 14-16. At Pearl Continental Hotel. Jan 19-21. et al. A .. Lahore. Q. Dubai.M. New Disease Reports.. Zafar. M.. Genome characterization of whitefly-transmitted geminiviruses of cotton and development of virus-resistant plants through genetic engineering and conventional breeding. f o l d i n g a n d y i e l d of recombinant proteins in both of the systems.571. Hussain. Faisalabad. Dubai 28-30 September2003. Haq. 2nd international Symposium on Biotechnology.. Anjuman.M.Y. M. Z. Khalid.. et al. 588.2003. PP. 21-21. Establishment of in vitro system for cotton regeneration and transformation. S. M. et al„ (2003). University of Arid Agriculture. June 13-18. Rawalpidi. Khan. et al. (2003). University of Sindh Jamshoro. S. 587. Biofertilizer Technology.A. Dubai.. Dubai 2830 September2003. Hussain.. (2003) "RT-PCR based Diagnosis of Infectious Bursal Disease Virus (IBDV) in Pakistan". Advanced Techniques in Biotechnology: A manual of an international NIBGE-COMSTECH (CPC) workshop. et al. et al. 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(2003). Pakistan 580. Y.. et al. M. ICBA. 2004 Georgia World Congress Center. Dubai 28-30 September2003. (2003). First report of tomato leaf curl New Delhi virus affecting chili pepper in Pakistan. P r e s e n t e d at N a t i o n a l E x e c u t i v e symposium on "Technologies developed for commercialization .M. R. A molecular insight into evolution and spread of cotton leaf curl disease complex.. S. 4th National Conference of Plant Pathology. Arabidopsis Vacuolar Pyrophosphatase (AVP1) induces Drought and Salt tolerance in Nicotiana tabacum plants.. Khan. 17. et ai. 583. "Molecular Detection of Infectious Bursal Disease Virus By Polymerase Chain Reaction In Pakistan" International Poultry Scientific Forum.. . M . 589. September 28-30.. M. April 2-5. (2003). Yasmin.. Lahore 584.. et al. 591. Dubai 28-30 September 2003.2004. First report of Mungbean yellow mosaic India virus on mungbean crop in Pakistan.94. 2004. 593. Pakistan 579. Punjab University. (2004). 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2nd International Oncology Conference/ 2nd PSNM meeting at INMOL. " R o l e of S a n d o s t a t i n in D i a r h e a " . Pakistan. Babar. 2003. A. "Serum PSA level in fertilizer factory workers" 1 st oncology conference 622. Tahir Nazir. 2004) 614. M. NCNDT Experience of Fracture Mechanics Based Condition Assessment of Pressurized Components. NCNDT Experience in Eddy Current Testing Technique for the Evaluation of Structural Integrity of Chemical and Power Plants.. M. Islamabad.. 72 Annual Report 2003-2004 . Pakistan. Sabir Chaudhary and Sultan Sikander. Ibid. Khan and M. and Saeeda Asghar. M. et al. M. 606. 39 (3-4) 2002. Analysis ofthe Mechanics of Fracture of Flanges in the Turbine Cross-Over Piping. Boilers and Condensers. 609. 608. et al. Islamabad. NUCLEAR MEDICINE Papers In Journals 605. Abid Hameed and Umardaraz. Communication Volume 25 (3). PP201-226 (March 2004).602. 621.A. PP201-226 (March 2004). etc. 603. Lahore) 612. PAEC and Dept Oncology UCL UK. pp. 624. "Evaluation of p-Fast for Evaluation of left ventricle volumes and ejection fraction". "Pandered Syndrome". Basit Iqbal and Abid Hameed. 2123 Islamabad. S o h a i l M u r a d . M. Ajmal. M. JCPSP 2003. 604. Afzal and M. 613. International Conference on Current Trends in Radiopharmaceuticals. 617. Afzal.M. Akhtar Ahmed "SPECT tumour volume (STV) in the assessment of t u m o u r response in patients with carcinoid Tumours" 2nd International PAEC Nuclear medicine conference (April 2004. 607. M. . An Acoustic Emission Investigation of Microfracture Processes in Nuclear Pressure Vessel Steel. The NUCLEUS. Khattak. "Effects of Cancer Chemotherapy on Harmones" ibid. "Quality Control of Radioiodinated Fatty Acids Using Modern Radioanalytical Techniques". 21 st to 23rd October. Khan. 629. et al. et al.I. 632. An Acoustic Emission Monitoring for Prevention of Failure in RCC Structures. 625. M . 626. "In vivo labeling of RBC with technetium-98"" ibid. PP 463464 (January 2004). K . 618. Standardization of Portable Non-Destructive X-ray Fluorescence Alloy Analyzer for InService Inspection. A. A comparison with ERNA and QGS. "Review of practical Aspects of magnetizable solid state separation t e c h n i q u e s in i m m u n o assays". Lahore. Tube Plugging Criteria for Heat Exchangers. The NUCLEUS. Khattak. RiffatA. e t a l . 2003. Abbas H. J Cardiovascular dis: 2(1). Rafia Toor and Abid Hameed. Babar. M. 630. Khan Q M (2003) T C . JCPSP 2003. PAK-KAZAKH-Project (984-1) submitted to MoST. " T h y r o i d hormones economy of normal person and patients with thyroid disease". symposium on "Recent Advances in Oncology" from 29-30 August. Failure Analysis of Valve Stem of Emergency Diesel Generator. et al.. 620. "Prediction of Clinical Outcome in Treated Neuroendocrine Tumours of Carcinoid type using functional volumes on 111 in Pentetreoide SPECT Imaging". A New Fracture Mechanics Method for Evaluation of Radiation Damage in Reactor Pressure Vessel for Remaining Life Assessment. dated: 8-10 April. et al. Khan Muhammad Sajid. 627. A. 11-14. "How much is enough in Near Total Thyroidectomy in case of Well Differentiated Carcinoma Thyroid for Effective Management with High Dose of Iodine 131". (JAN.13 (8). Third Party. M. SCIENTIFIC AND ENGINEERING SERVICES 623. Khan Q M (2003) Final Technical Report on biosorption based removal of heavy metals from Industrial effluents. Sajid.. Khan.I A E A (PAK/5/041.G. Pakistan Institute of Medical Sciences. " R e c e n t Advances in Brest Cancer Management".. "Review of practical Aspects of magnetizable solid state separation techniques in immunoassays". Muhammad Ali. Sabir Chaudhary and Sultan Sikander. 610. 2004. A f z a l .) Technical Report on Setting up of PCR and immunoassay based methods for diagnosis and control of rinderpest in Pakistan submitted to IAEA Vianna. Akhtar Ahmed. March 2004. Ibid. 39 (3-4) 2002. Khan. Khan Muhammad Sajid.13 (8). Non-Destructive Testing Activities buring In-Service Inspection at a Power Plànt. et al. 628. "Delay in diagnosis of Breast Masses". A. D u b a i . Afzal. M. 631. S h a h i n a P a r v e e n . 14(5). Pasha and M. Israr Ahmad. 619. 616. 615. "Myocardial Perfusion Scintigraphy in Patients with Type II Diabetes Mellitus Presenting with Non-specific Cardiac Symptoms". "Prostatic Specific Antigen levels in fertilizer factory workers" JCPSP May 2004. "Pandered Syndrome". Conference Presentations 611. A. Austria. M u h a m m a d S a j i d .I. 290-293. international conference on current trends on pharmaceuticals October. pp 253-257. Sajid. PP 463464 (January 2004). symposium on "Recent advance in the Management of Carcinoma Breast" arranged by department of general surgery. K h a n QM ( 2 0 0 3 ) T e c h n i c a l Report-1 on "Use of molecular and cellular techniques to monitor DNA damage and repair in human cell lines exposed to DNA damaging agents" Submitted to HEC.

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