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ZR Fungal/Bacterial DNA Kits

Simple, Rapid Isolation of DNA from Tough-To-Lyse Fungi & Bacteria


P Isolate PCR-quality DNA from fungi and bacteria P Efficient processing with ultrahigh density BashingBeads P Does not require the use of organic denaturants or lytic enzymes (e.g., Zymolyase)
The ZR Fungal/Bacterial DNA MicroPrep, ZR Fungal/Bacterial DNA MiniPrep, ZR Fungal/ Bacterial DNA MidiPrep, and ZR-96 Fungal/ Bacterial DNA Kit are designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedures are easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Fast-Spin column or plate technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.

Specifications Sample Sources


Gram (+) bacteria, Gram (-) bacteria, Yeast, Filamentous fungi, Unicellular algae, Filamentous algae, Protists
Our state of the art BashingBeads are constructed of the highest quality, most dense ceramic material available today. They are used when thorough sample homogenization/lysis is required by the researcher. DNA shearing by physical and chemical methods is minimized since the beads are fracture resistant and chemically inert. They are unique amongst the lysis matrices offered by other companies for DNA isolation from tough-to-lyse materials.

DNA Binding Capacities

ZR Fungal/Bacterial DNA MicroPrep: 5 g/prep. ZR Fungal/Bacterial DNA MiniPrep: 25 g/prep. ZR Fungal/Bacterial DNA MidiPrep: 125 g/prep. ZR-96 Fungal/Bacterial DNA Kit: 5 g/well.

Zymo Research 1 kb Yeast Spores E. coli

Supplier M Yeast Spores E. coli

Processing Times

ZR Fungal/Bacterial DNA MicroPrep: 10 min. ZR Fungal/Bacterial DNA MiniPrep: 10 min. ZR Fungal/Bacterial DNA MidiPrep: 20 min. ZR-96 Fungal/Bacterial DNA Kit: 40 min.

DNA Purity
A260/A280 > 1.8

DNA isolated from S. cerevisiae (spores) and E. coli using the ZR Fungal/ Bacterial DNA MiniPrep is high quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the ZR Fungal/Bacterial DNA MiniPrep or the kit from Supplier M. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research).

Input Kit Sample wet weight 10 - 20 mg 50 - 100 mg 250 - 500 mg 10 - 20 mg/well Bacterial cells 2 x 108 10
9

Yeast cells 2 x 107 10


8

Soft Tissue 40 mg 200 mg 500 mg 40 mg/well

Toll-Free Main Fax Email Web

1-888-882-9682 1-714-288-9682 1-714-288-9643 info@zymoresearch.com www.zymoresearch.com

ZR Fungal/Bacterial DNA MicroPrep ZR Fungal/Bacterial DNA MiniPrep ZR Fungal/Bacterial DNA MidiPrep ZR-96 Fungal/Bacterial DNA Kit

5 x 109 2 x 10 /well
8

5 x 108 2 x 10 /well
7

ZR Fungal/Bacterial DNA Kits


MicroPrep & MiniPrep Formats MidiPrep Format

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Pulverize Sample with ZR BashingBead Lysis Tube

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Pulverize Sample in ZR BashingBead Lysis/Filtration Tube

Filter Lysate with Zymo-Spin IV

Centrifuge & Transfer Lysate to Zymo-Spin V-E w/ Zymo-Midi Filter

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OR Bind, Wash, Elute DNA with Zymo-Spin IC or IIC

$ $
Spin Wash Elute

PCR Ready, Ultra-pure DNA

ZR Fungal/Bacterial DNA MiniPrep Short Protocol


1. Add sample to a ZR BashingBead Lysis Tube & process in a cell disruptor at maximum speed for 5 min. 2. Centrifuge the ZR BashingBead Lysis Tube at 10,000 x g for 1 min. 3. Transfer 400 l supernatant to a Zymo-Spin IV Spin Filter in a Collection Tube & centrifuge at 7,000 rpm for 1 min. 4. Add 1.2 ml Fungal/Bacterial DNA Binding Buffer to the filtrate in the Collection Tube from Step 3. 5. Transfer 800 l of the mixture from Step 4 to a Zymo-Spin IIC Column in a Collection Tube & centrifuge at 10,000 x g for 1 min. Discard the flow through & repeat. 6. Add 200 l DNA Pre-Wash Buffer to the Zymo-Spin IIC Column & centrifuge at 10,000 x g for 1 min., then wash w/ 500 l Fungal/Bacterial DNA Wash Buffer. 7. Transfer the column to a clean 1.5 ml microcentrifuge tube & add 100 l DNA Elution Buffer directly to the column matrix. Centrifuge at 10,000 x g for 30 sec. to elute the DNA.

PCR Ready, Ultra-pure DNA

96-Well Format

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Pulverize Samples with ZR BashingBead Lysis Rack

96-well Block

$ $
PCR Ready, Ultra-pure DNA Bind, Wash, Elute DNA with Silicon-A Plate

Ordering Information Product Description ZR Fungal/Bacterial DNA MicroPrep ZR Fungal/Bacterial DNA MiniPrep ZR Fungal/Bacterial DNA MidiPrep ZR-96 Fungal/Bacterial DNA Kit Catalog No. D6007 D6005 D6105 D6006 Kit Size 50 preps. 50 preps. 25 preps. 2 x 96 preps.

Note - Trademarks of Zymo Research Corporation. This product is intended for research use only and should only be used by trained professionals. This product is not intended for use in diagnostic procedures. Some reagents included with this kit are irritants. Wear protective gloves and eye protection. Follow the safety guidelines and rules enacted by your research institution or facility.