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ANIMAL GENETICS & BREEDING

Course Name: Animal Cytogenetics and Immunogenetics Course No: AGB- 601 Credits: 2+1=3 THEORY Lecture No 1 2 3-4 5 6 7 8-9 10-11 12-13 14 15 16-17 18 19 20 21 22 23 24-25 26 27-28 29 30-31 32 Topic Development of animal cytogenetics in farm animals Development of animal immunogenetics in farm animals Immunoglobulin and their types Antigen-antibody interactions Immune response ELISA Major Histocompatibility comples Genetics of Biochemical variants and their applications Ir genes and concept of disease resistance including major genes Hybridoma and its significance Concept of immuno-fertility BoLA, BuLA, TLRs, Interlukins Chromatin structure of eukaryotes Chromosome number and morphology in farm animals Karyotyping Chromosomal and genetic syndromes DNA packing in chromosomes Z & B DNA FISH chromosome panting and PRINS RH Panel mapping Mutation and assays of mutagenesis Sister chromatid exchanges Recombinant DNA techniques Applications of Recombinant DNA techniques PRACTICALS Practical No 1 2 3 4 5-6 7 8-9 10 11 12-13 14-16 Topic Polumorphism of Haemoglobulins Polymorphism of Transferrins Polymorphism of Enzymes/ Proteins Preparation of monovalent blood reagent- isoimmunization Titre testing and absorption of polyvalent serum Identification of bar bodies In-vitro and in-vivo preparation of somatic metaphase chromosomes Screening of chromosomal abnormalities Microphotography and Karyotyping Banding procedures for comparing the chromosomal complement FISH and PRINS

ANIMAL GENETICS & BREEDING


Course Name: Molecular Genetics in Animal Breeding Course No: AGB- 602 Credits: 2+1=3 THEORY Lecture No 1 2 3-4 5 6-7 8 9 10 11 12 13 14 15 16 17 18 19-20 21-22 23 24-25 26-27 28-29 30-31 32 Topic Basic concepts of Molecular genetics Genesis and importance of molecular techniques Genome organization Physical and genetic map Current status of genome map in livestock Molecular markers and their applications RFLP marker RAPD marker Microsatellite/ minisatellite marker SNP marker DNA fingerprinting DNA sequencing Genome sequencing Genomic library Polymerase chain reaction Types of Polymerase chain reaction Applications of Polymerase chain reaction Transgenesis and methods of gene transfer Statistical techniques for analyzing molecular genetic data Quantitative trait loci QTL mapping and its application in animal breeding Genome scan Candidate gene approach Genomic selection Marker Assisted Selection- basis PRACTICALS Practical No 1-2 3-4 5-6 7-8 9 10 11-12 13 14 15 16 Topic Extraction and purification of genomic DNA Gel electrophoresis Restriction enzyme digestion of DNA and analysis PCR PCR-RFLP PCR-SSCP Bioinformatics for DNA sequence analysis Design of primer Isolation of RNA cDNA synthesis Statistical methods for analyzing molecular genetic data

Sr. M. V. Sc. No 1 2 3 Name Amit Kini Sushil Chavan Namrata Nakod Jr. M. V. Sc. No 1 2 3 Name Amol Khade Suresh Kad Pranav Ware Contact 08080449956 08080449942 09820611369 Contact 09892627362 09975753085 09421091347

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