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Basic University Training in Biological & Biomedical Science

Escherichia Genomics P.Ltd


Mobile: 91+9716159717, 91+ 9560757331, 91+ 011-43090505
Email: , Escherichia4@gmail.com Info@escherichiagenomics.com
Escherichia Genomics
Escherichia Genomics P.Ltd feels that the biotechnology, life science and biomedical sciences required customized, flexible, cost effective, and convenient education,
training and consulting for organization. We make our biotechnology, life science and biomedical training courses to prepare and maintain the knowledge, skills and
abilities of biotechnology, life sciences and biomedical professionals through trained quality individuals with diverse skills needs. The training programme includes
changes in mindset and thinking patterns, eliminating fears and identifying strengths and talents. Strengthening the moving intelligence of a person is a fundamental
part of this programme. We can help you: Stay competitive, upgrade skills, prepare to for start-up, increase productivity, promote continuous improvement,
Apply online : www.escherichiagenomics.com
MODULE: 1. HANDS ON: 15 DAYS
Molecular Marker: (RAPD, ISSR) Gel Preparation Electrophoresis,
Gel analysis, RAPD, ISSR, SSR, STR, AFLP, Etc
DNA PCR Image: Image Preparation, Scoring of Amplicons /
Binary Preparation,
Allele,
Data Analysis:
A. Monomorphic Allele Frequency, Polymorphic Allele Frequency,
B. Average Amplicons, Unique Allele, Private Allele, Fix Allele,
C. Diversity Coefficient (Distance Matrix)
D. Jaccard`S Diversity Coefficient
E. UPGMA / SHAN Cluster
F. Neighbor Joining Methods
G. Dendrogram/phylogentic tree
H. Principle coordinate analysis
I. Banding Pattern, Application Of Banding Pattern,
J. Primer Discriminatory Power,
K. Polymorphic Information Content (PIC Value)
L.Effective Multiplex Ratio (EMR)
M. Marker Index / Multiplex Ratio (MR)
N. Average Heterozygosity
O. Resolving Power (Rp)
P. Band Informativeness
Software Analysis:
A. NTsys PC program, B. FAMD, C. Darwin,
D. Mega 5 E. POPgene G. Metapiga & other important software in use.
MODULE: 2. HANDS ON: 5 DAYS
Fundamental Principle of DNA Barcoding
Barcode Data Standard Compliance
A. Vouchering and archiving of vouchers
B. Imaging and archival of e-vouchers
C. Provenance data quality
D. Sequence and trace file quality
E. Acquisition and Handling of Specimens
Field Collection
A. Legal issues
B. Logistics
C. Data quality and acquisition
D. Collection/preservation methods
Museum Harvesting
A. Deciding which collections to use
B. Deciding which specimens/samples to use
C. Destructive vs. non-destructive handling;
D. Voucher recovery protocols
E. Biological material transfer policies
Laboratory Methods and Information Management
DNA Extraction
A. Equipment
B. Methods/protocols
C. Storage and shipment of DNA extracts
PCR Amplification
A. Equipment
B. Methods/protocols
C. PCR product verification
D. Shipment of PCR products
Taxon-specific aspects of the barcoding
a. Plants b. Algae c. Fungi
d. Marine invertebrates e. Terrestrial invertebrates and Vertebrate
Protein Techniques (Principle, Methods and Protocols)
1.Protein Isolation:
a.Protein Isolation Principle and Methods
b.Bacterial Protein Isolation
c.Plant Protein Isolation
2.Protein SDS-PAGE Electrophoresis
a.Solution Preparation
b.Resolving Gel Preparation
c.Stacking Gel Preparation
d.Sample Preparation
e.Gel Casting
f.Sample Loading
g.Gel Staining
h.Gel destaining
i.Gel Documentation and Analysis.
A.How To Write A Molecular Marker/SDS-Page/Barcoding And
DNA Fingerprinting Research Paper. (Exercise)
1.How To Write Abstract 2.How To Write Introduction
3.How To Write Material And Method 4.How To Write Result And Discussion
5.How To Write Reference 6. How To Write Acknowledgment
MODULE: 3. HANDS ON: 5 DAYS
RAPD Profile
Dendrogram
Barcode of Binary
Eligibility
Professionals from Private/Govt./NGOs/University / Faculty, Research scholars,(Ph.D Student), and Student from B.Tech/
M.Sc/ M.Tech/ M.V.Sc in Biotechnology, Molecular & Human Genetics, Molecular Biology & Biotechnology, Neuro-Science,
Biochemical Engineering & Biotechnology, Bio-process Technology, General/ Agricultural/ Industrial/ Marine/ Medical/
Pharmaceutical/Environmental/Plant/ Food/Animal Biotechnology Forensic life sciences and applied discipline are eligible
to apply.
Fee:
1. Indian Candidate:
2. Foreign Candidate:
Escherichia Genomics P.Ltd
INR 9500/per student and INR 10,000/per faculty.
USD 1000/per student and USD 1200/per faculty. (Including
Accommodation and Fooding)
3. Payment Option : Cheque / Online Transfer / DD in favour of
Contact Person
V.K. Singh
Managing Director: Project and Training
Escherichia Genomics P.Ltd
Mobile: 91+9716159717, 91+ 9560757331, 91+ 011-43090505
Email: , Escherichia4@gmail.com Info@escherichiagenomics.com
Basic Molecular Biology Techniques
Overview Introduction: Laboratory Etiquette,
Molecular Biology Techniques, Laboratory Protocols,
DNA Techniques (Principle, Methods and Protocols)
1.DNA Isolation from Different Cell and Tissue
2.DNA Purification Isolated from Different Cell and Tissue
3.DNA Quantification methods
4.DNA Agarose Electrophoresis
5.DNA Dilution for Restriction, PCR and Ligation Reactions
6.PCR Reaction Cocktail Preparation, PCR Program.
7.PCR product Electrophoresis, PCR Result Analysis
Plasmid DNA Techniques (Principle, Methods and Protocols)
1.Plasmid DNA Isolation from Different Cell line
2.Plasmid DNA Purification Isolated from Different Cell line
3.Plasmid DNA Agarose Electrophoresis
4.Plasmid DNA Dilution for Restriction, PCR and Ligation reactions
Organelles DNA Techniques (Principle, Methods and Protocols)
1.Chloroplast DNA Isolation from Different Cell and tissue
2.Mitochondrial DNA Isolation from Different Cell and tissue
RNA Techniques (Principle, Methods and Protocols)
1.RNA Isolation from Different Cell and Tissue
2.RNA Purification Isolated from Different Cell and Tissue
3.RNA Quantification Methods
4.RNA Agarose Electrophoresis
5.RNA Dilution for PCR Reactions
6.RNA Reaction Cocktail Preparation, PCR Program.
7.PCR Product Electrophoresis , PCR Result analysis
PCR Techniques (Principle, Methods and Protocols)
1.Type of PCR , Multiplex-PCR, Nested PCR, Quantitative PCR,
Hot-start PCR, Touchdown PCR, Assembly PCR, Colony PCR,
RT-PCR (or Reverse Transcription PCR), Ligation-mediated PCR,
Methylation-specific PCR (MSP) etc
2.Primer Designing and Primer Modifications
3.Primer Annealing Temperature, GC Content , Gradient PCR
4.PCR optimization:(magnesium ion (Mg++), Denaturants
(such as DMSO, glycerol) and Triton X-100)
5.PCR Reaction Cocktail Preparation, PCR Program.
6.PCR Result and Analysis
Molecular Marker and DNA Fingerprinting
(Software and Statistical Analysis)
DNA BARCODING
RESEARCH PAPER WRITING
SDS-PAGE PROFILE
1 2 3 4 M
Jaccard Coefficient
PCA
X
Y
Z -0.40
-0.40
-0.40 -0.30
-0.30
-0.30 -0.20
-0.20
-0.20 -0.10
-0.10
-0.10 0.00 0.00 0.00 0.10
0.10
0.10 0.20
0.20
0.20 0.30
0.30
0.30 0.40
0.40
0.40 Apis mellifera-Illustrative DNA Barcode
Accommodation : manage by trainee, while our team will assist in getting the accommodation near laboratory premise ( p.g., hostel a/c or non a/c )
: 9716159717
28 S RNA
18 S RNA
5 S RNA
RNA ELECTROPHORESIS
PCR Machine Programming
& Operation
Start from 5th Oct. 2014 to 30th Oct. 2014 (Last Date to Apply 30th Sep. 2014)