Code No: RR322306

Set No. 1

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 PROTEIN ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write short notes on: (a) Edman degradation (b) Identifying Amino terminal and Carboxy terminal residues. [8+8]

2. Justify the statement that “tertiary structure is more organized than secondary structure”. [16] 3. Define post-translational Modification? Explain Post-translational modification by alteration of chain termini? [16] 4. Describe the zinc finger and their function. [16]

5. Name the membrane protein which is present in Halobacterium salinarium ? Explain about mechanisms of light driven proton pump in Bacteriorhodopsin? [16] 6. Discuss the structure of Insulin and describe its functions. [16]

7. Write about Pattern databases for storing information about protein families and motifs? [16] 8. (a) What do you mean by protein design? (b) What are the goals of protein design? ⋆⋆⋆⋆⋆ [8+8]

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Code No: RR322306

Set No. 2

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 PROTEIN ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Describe the characterization of protein structure? 2. Describe the role of hydrophobic forces in tertiary structure. 3. (a) What are chaperons? (b) Describe types of chaperons and their role in folding of protein. 4. Describe the zinc finger and their function. [8+8] [16] [16] [16]

5. Describe the properties of three classes of Membrane proteins, how they differ from one other and how they vary among themselves? [16] 6. Describe the sequence of events following the absorption of photon of light by photosynthetic reaction center pigment of PS-II. Describe the comparable events in PS-I. How are two photosystems linked to one another? [16] 7. Write a note on Curated family databases for Protein? 8. (a) What do you mean by protein design? (b) What are the goals of protein design? ⋆⋆⋆⋆⋆ [8+8] [16]

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Code No: RR322306

Set No. 3

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 PROTEIN ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Explain Secondary structure prediction from protein sequences? 2. Describe the role of hydrogen bonds in tertiary structure of protein. 3. Describe Posttranslational modifications by proteolytic cleavage. [16] [16] [16]

4. Explain in detail about the different Transcription factors that contains Zinc as an essential element of their DNA binding domains? [16] 5. (a) What are proteases. (b) Describe different classes of proteases with examples. (c) Explain the action of serine proteases in details. [5+6+5] [16]

6. Explain the process of recognition of foreign molecule by Immunoglobulins.

7. Write a note on engineering complex human proteins by large scale mammalian cell culture? [16] 8. Explain the importance of protein designing in the field of Drug designing. ⋆⋆⋆⋆⋆ [16]

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Code No: RR322306

Set No. 4

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 PROTEIN ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Discuss about General properties of Proteins Secondary structure? 2. Write short notes on: (a) Myoglobin (b) Globular proteins [8+8] [16]

3. Justify statement “Final structure of protein is not determined by Gene structure but by the primary structure of protein”. [16] 4. Write short notes on: (a) Zinc fingers (b) Leucine zippers [8+8]

5. Name the membrane protein which is present in Halobacterium salinarium ? Explain about mechanisms of light driven proton pump in Bacteriorhodopsin? [16] 6. (a) What are Immunoglobulins. (b) Describe the structure of Immunoglobulin. (c) Describe the various classes of Immunoglobulins and their properties. [5+5+6] 7. Write short notes on: (a) PROSITE (b) BLOCKS 8. (a) What do you mean by protein design? (b) What are the goals of protein design? ⋆⋆⋆⋆⋆ [8+8] [8+8]

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