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Some simple conversions: 1 mol = 1000 mmol 1 mmol = 1 x 10-3mol 1 pmol = 1 x 10-12mol 1 L = 1000ml 1 g = 1000mg

1 mmol = 1000 mol 1 mol = 1 x 10-6mol 1ml = 1000 l 1 mg = 1000 g

1 umol = 1000 nmole 1 nmol = 1 x 10-9mol 0.1 ml = 100 l 0.01 mg = 10 g

1M solution = 1000 mmoles / dm3 1000 mmoles / L 1000 mmoles / 1000 ml 5 mg/ml 1 mg/ml This is a 5X dilution i.e 1 part original stock solution given : 4 parts diluent / water / buffer Common formula used : C1V1 = C2V2 1% (w/v) solution x: 1 g x dissolved and made up to 100 ml in water or buffer 1% (v/v) solution x: 1 ml x is made up to 100 ml in water or buffer Worksheet 1. Express the following in the units given:a) 0.25 ml = _____250_______ l b) mol c) d) e) mls 2. You are asked to make 50 mls of a 1.2 M sucrose solution. You would add _20538____mg of sucrose and dissolve it in water to a total volume of 50 mls. (MW of sucrose = 342.3) You are given a 25 mg/ml solution of BSA. You need 200 g to carry out an assay. How much would you use? ____8________ l Given a 100% absolute ethanol solution, your professor asks you to dilute it down to 20%, to a total volume of 200 ml. 10 mg/ml 5% KCl 0.09% NaCl = _____10_______ g/ l = ___5_ g KCl dissolved in water to a total volume of 100 mls = ___0.018__g NaCl dissolved in water to a total volume of 20 750 pmoles = ____0.75________ nmol = ___0.00000000075__________

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i) ii) 5.

How many times dilution is that? _____5________ You need to add ______40______ ml of the 100% ethanol given, and top it up with ___160______ml of water to achieve a final concentration of 20%. The laboratory that you are working in has a bottle of sterile 1 M MgCl2. You need to make a potassium phosphate buffer containing 50 mM MgCl2 that is compatible with your enzyme assays. You would need 50 mls of this for your enzyme assays. i) ii) iii) 50mM = ___0.05______ M This requires a ____20____ times dilution of the original 1 M MgCl2 stock. To make 50 mls of this enzyme buffer, you would add __2.5_____ mls of 1 M MgCl2 and top it up with __47.5_____mls of potassium phosphate buffer to a final volume of 50 mls.

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You need to make a 0.8% agarose gel in electrophoresis (TAE) buffer with 0.5 g/ml ethidium bromide for DNA analysis. You are provided with 0.4 g agarose powder and a 10mg/ml stock of ethidium bromide. i) ii) You would add ____50____ml TAE buffer to 0.4g of agarose powder weighed out for you. To that, you would add ___2.5______ l of 10 mg/ml ethidium bromide.

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Your research team is about to carry out protein estimation. To do that, you would need to first set up a series of BSA (bovine serum albumin) standards for use in your assay. Provided with a 1 mg/ml BSA stock, set up your standards as given in the table below. Fill in the blanks.

BSA standard 0 g/ml 5 g/ml 8 g/ml 10 g/ml 12.5 g/ml 15 g/ml 20 ug/ml 35 g/ml 50 g/ml

BSA stock ( l) 0 l 5 l 8ul 10ul 12.5 l 30 l 19.6ul 35 l 0.5 ml (500 l)

Water ( l) 1 ml 995ul 992 l 0.99 ml 987.5ul 1970ul 960 l 965ul 9.5ml

Final volume 1 ml 1 ml 1 ml 1ml 1 ml 2 ml 979.6ul 1ml 10ml