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Supplementary readings Please be aware of the proprietary and be critical about the contents.

Introduction of E. coli http://www.science.siu.edu/microbiology/micr425/Index.html#contents http://ecoli.bham.ac.uk/ http://openwetware.org/wiki/E._coli_genotypes#DH5.CE.B1 (genetic mutant DH5) Phenol and DNA extraction http://www.ambion.com/techlib/tb/tb_158.html http://www.madsci.org/posts/archives/may2000/957538977.Mb.r.html Precipitation of DNA http://omrf.ouhsc.edu/~frank/ETOHPPT.html http://tools.neb.com/wolbachia/labsite/protocols/DNAPRECIPITATION.htm DNA gel electrophoresis http://www.life.uiuc.edu/molbio/geldigest/electro.html Plasmid http://www.fermentas.com/techinfo/nucleicacids/mappuc1819.htm BioTechniques Molecular Biology Techniques Forums http://molecularbiology.forums.biotechniques.com/forums/index.php Medium http://www.mc.uky.edu/oaa/curriculum/iid98/manual/00lab1.htm(MacConkey plate)