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for laboratory research use only, not for

Ubiquitin (P4G7) Monoclonal Antibody human or in-vivo use. Covance antibodies
Catalog Number: MMS-258R may not be resold or modified for resale
Available Size: 0.2 mL, 0.5 mL without prior written approval.
▪ Western blot: 1:1,000*

Notes: *Predicted MW = 9 kD
Description: Monoclonal Antibody against
Ubiquitin Storage: Store at -20°C. Upon initial thawing,
apportion into working aliquots and store at -
Clone: P4G7 20°C. Avoid repeated freeze-thaw cycles to
prevent denaturing the antibody. For long-
Form: Ascites term storage, keep the antibody at -80°C.

Host: Mouse References: Kahana A, Gottschling DE. DOT4
Links Silencing and Cell Growth in
IsoType: IgG1 Saccharomyces cerevisiae. Mol. Cell. Biol.
19(10):6608-6620, 1999.
Species Reactivity: Extensive (Yeast to
Human) Hochstrasser M. Ubiquitin-dependent protein
degradation. Annu. Rev. Genet. 30:405-439,
[Ab]: 3-5 mg/mL 1996.

Specificity: Antibody P4D1 was raised against Related Products:
denatured bovine ubiquitin and recognizes
ubiquitin, polyubiquitin, and ubiquitin- ▪ Ubiquitin (P4D1) Monoclonal Antibody
conjugated proteins. MAb P4D1 is equivalent to Catalog Number MMS-257P
MAb P4G7. The ubiquitin protein is extremely
well conserved and thus the antibody has ▪ E1 Ubiquitin Activating Enzyme,
extensive species cross-reactivity from yeast Monoclonal Antibody
to human. This antibody was specifically Catalog Number MMS-259R
developed to detect ubiquitin and ubiquitin-
substrate conjugates by immunoblotting. ▪ Ubiquitin Ubi-1 Monclonal Antibody
Because the antibody recognizes a denatured Catalog Number MMS-264R
epitope, it is known not to work in
immunoprecipitation or immunostaining ▪ Ubiquitin Polyclonal Antibody
applications. Catalog Number PRB-268C

Uses: This antibody is effective in ▪ UBE1L N-Terminal Polyclonal Antibody
immunoblotting and ELISA. Catalog Number PRB-271C

Suggested Working Dilution: The optimal ▪ UBE1L C-Terminal Polyclonal Antibody
working dilution should be determined for each Catalog Number PRB-272C
specific assay condition. This antibody is sold

800 University Avenue •Berkeley, California • 94710-2021
(p) 800 / 922-2226 • (f) 510 / 704-2000 • (e) ab.products@CRPinc.com • (w) http://www.CRPinc.com
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Cont. of Ubiquitin (P4G7) Monoclonal Antibody, MMS-258R

Product Revision Date: 11/27/2006

800 University Avenue •Berkeley, California • 94710-2021
(p) 800 / 922-2226 • (f) 510 / 704-2000 • (e) ab.products@CRPinc.com • (w) http://www.CRPinc.com
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