Professional Documents
Culture Documents
Your Task
As a chemical technician at Water Wagon Laboratories, you are responsible for taking the Cu2+(aq) (aqueous copper(II) solution) and determining the amount of copper present in the solution. To accomplish this task, you must measure the absorbance spectrophotometer. of several standard copper solutions using a
prepare a linear graph from this data. measure the absorbance of the ore sample solutions. use the graph to determine the concentration of copper in the ore sample solutions.
Materials
Per lab team Spectrophotometer (Spec-20 or Genesys-20) Cuvettes (6 or more) A set of Cu2+ (aqueous copper(II) sulfate solution) standards (5.00 g/L, 4.00 g/L, 3.00 g/L, 2.00 g/L, 1.00 g/L) 1 box lintless paper wipes
running water. Wash your hands after use. Follow local ordinances for disposal of copper(II) salts. It is recommended that unused copper(II) sulfate solution be left to evaporate to dryness. Follow any additional disposal procedures as outlined by your instructor.
Using a Spectrophotometer
A spectrophotometer is used to make absorbance measurements. Light passes into a monochromator (where only the desired wavelength, or a very narrow range of wavelengths, can pass through). The light then passes through the sample, and on to a phototube, where the light energy is converted to an electrical current that is registered on a meter.
Procedure
1. 2. 3. Use a disposable pipette to fill a cuvette approximately full of water. Wipe off the cuvette with a lintless paper wipe. Place the cuvette containing water into the sample
compartment and align the lines. This is the reference solution (often called the blank). 4. Close the cover and set the absorbance to 0 by moving the lower right hand dial. 5. 6. Remove the cuvette of water. Fill a cuvette approximately full of one of the standard solutions. It would be
appropriate to begin with the 5.00 g/L Cu2+, copper(II) solution. 7. 8. 9. 10. 11. Wipe off the cuvette with a lintless paper wipe. Place the cuvette in the sample compartment and align the lines. Close the cover and record the absorbance in the data table below. Remove the cuvette. Repeat steps 6-10 to measure the absorbance of the other standard solutions (4.00 g/L, 3.00 g/L, 2.00 g/L, and 1.00 g/L). 12. 13. 14. 15. 16. 17. 18. 19. Fill a cuvette approximately full of Chrysocolla solution. Wipe off the cuvette with a lintless paper wipe. Place the cuvette in the sample compartment and align the lines. Close the cover and record the absorbance. Remove the cuvette. Fill a cuvette approximately full of Malachite solution. Wipe off the cuvette with a lintless paper wipe. Place the cuvette in the sample compartment and align the lines. Close the cover and record the absorbance. Remove the cuvette.
Data Table
Concentration Solution (g/L) A B C D E Chrysocolla Sample Malachite Sample 5.00 4.00 3.00 2.00 1.00 Absorbance
0.8
0.6
Absorbance
0.4
0.2
1.0
2.0
3.0
4.0
5.0
Concentration (g/L)