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Foodborne Bacteria www.neogen.com/foodsafetyorder.htm

GENE-TRAK®
For Salmonella DLP Assay

Results

Positive Negative Positive Negative


Control Control Sample Sample

Product Specifications
Sensitivity: 1-5 CFU/25g
Testing time: 1.5 hours
(after 40-48 hour enrichment)
Test per kit: Up to 98
Intended Use Approvals: AOAC-RI #961101
GENE-TRAK Salmonella Direct Labeled Probe (DLP) Assay provides
for the detection of Salmonella spp. in food. Materials Required But Not Provided
Available from Neogen*
The Test 1. GENE-TRAK photometer set
The DNA hybridization test employs Salmonella-specific DNA probes 2. Test tube racks
directly labeled with horseradish peroxidase and a colorimetric detection 3. Dipstick holders
system for the detection of Salmonella in food samples following broth 4. Wash basins with lids
culture enrichment. A sample is considered negative for the presence of 5. Lactose broth and/or other pre-enrichment media
Salmonella if the absorbance value obtained is less than or equal to the 6. Tetrathionate broth
established cutoff value for the assay. A sample is considered presump- 7. Selenite cystine broth
tively positive for the presence of Salmonella if the absorbance value 8. Gram negative broth
obtained is greater than the established cutoff value of the assay. 9. Pipettes (1 mL and 10 mL)
10. Incubator at 36°C ± 1°
Test Procedure 11. Incubator at 42°C ± 1°
1. Enrich food samples. 12. Heater block at 37°C and 65°C
2. Add 0.2 mL of enriched samples (0.1 mL from each culture tube), 13. 12 x 75 mm glass test tubes
negative control and positive control into separately labeled 12 X 14. Minute timer
75 mm glass tubes. 15. Micropipette and tips, 200 – 1000 µL range (optional)
3. Add 0.1 mL lysis reagent to each sample tube. Shake for 5 seconds
and incubate at 65°C for 5 minutes. Not available from Neogen
4. Add 0.4 mL of hybridization solution and 0.2 mL of Salmonella 1. Blender or homogenizer
probe solution to each tube. 2. Culture bottles for sample pre-enrichment
5. Place dipsticks into sample tubes. Raise and lower dipsticks 10 3. Culture tubes for 11 mL sample volume
times to mix, and incubate at 37°C for 1 hour. 4. Graduated cylinder
6. Wash dipsticks two times for 1 minute each at room temperature. 5. Absorbent paper
7. Transfer dipsticks to tubes containing 0.75 mL of substrate-chro- * See Equipment and Accessories, pages 91–95
mogen and incubate at room temperature for 20 minutes.
8. Remove dipsticks and add 0.25 mL stop solution.
9. Read results using a GENE-TRAK photometer.
See package insert for complete instructions

Ordering Information
Prod.# Product description
6702 GENE-TRAK Salmonella DLP Assay - up to 98 tests

Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com 49