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Foodborne Bacteria www.neogen.com/foodsafetyorder.htm

GENE-TRAK®
Staphylococcus aureus Assay

Results

Positive Negative Positive Negative


Control Control Sample Sample

Product Specifications
Sensitivity: 1-5 CFU/25 g
Testing time: 3 hours (after 24-hour enrichment)
Tests per kit: Up to 98
Intended Use
GENE-TRAK Staphylococcus aureus Assay provides for the detection Materials Required But Not Provided
of S. aureus in food. Available from Neogen*
1. GENE-TRAK photometer set
The Test 2. Test tube racks
The DNA hybridization test employs S. aureus–specific DNA probes and 3. Dipstick holders
a colorimetric detection system for the detection of S. aureus in food 4. Dilution chart
samples following broth culture enrichment. The method is a semi- 5. Wash basins with lids
quantitative test to identify the presence of S. aureus at contamination 6. Pipettes (1 mL – 10 mL)
levels greater than 3 cells per gram in the original food sample. A sample 7. Incubator at 35°C
is considered non-reactive for the presence of S. aureus if the absorbance 8. 12 x 75 mm glass test tubes
value obtained is less than or equal to the established cutoff value for 9. Minute timer
the assay. A sample is considered reactive for the presence of S. aureus 10. Micropipette tips, 100-1000 mL range
if the absorbance value obtained is greater than the established cutoff
value for the assay. Not available from Neogen
1. Blender or homogenizer
Test Procedure 2. Culture bottles to hold 60 mL sample volume
1. Enrich food samples. 3. Modified peptone
2. Add 0.5 mL of enriched samples, negative and positive controls 4. Modified Giolitti-Cantoni broth
into separate 12 X 75 mm glass tubes. 5. Water bath at 37°C
3. Add 0.1 mL pretreatment reagent to each tube. Shake the tubes for 5 6. Water bath at 65°C
seconds, then incubate the tubes in 37°C water bath for 30 minutes. 7. Graduated cylinder
4. While in water bath add 0.1 mL lysis reagent to each test tube. 8. Absorbent paper
Remove rack of tubes and shake for 5 seconds. Replace in bath 9. Test tubes, sterile with caps to hold 3 mL sample volume
and incubate in 37°C water for 45 minutes. * See Equipment and Accessories, pages 91–95
5. Add 0.1 mL of S. aureus probe solution to each tube.
6. Place dipsticks into sample tubes. Raise and lower dipsticks 5 times
to mix and incubate at 65°C for 1 hour.
7. Wash dipsticks two times for 1 minute each, first at 65°C, then at
room temperature.
8. Transfer the dipsticks to tubes containing 0.75 mL of enzyme
conjugate and incubate at room temperature for 20 minutes.
9. Wash the dipsticks two times for 1 minute each at room temperature.
10. Transfer dipsticks to tubes containing 0.75 mL of substrate-chro-
mogen and incubate at room temperature for 20 minutes.
11. Remove the dipsticks and add 0.25 mL of stop solution.
Ordering Information
12. Read results using a GENE-TRAK photometer.
Prod.# Product description
See package insert for complete instructions
6715 GENE-TRAK Staphylococcus aureus Assay – up to 98
tests

Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com 53