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Foodborne Bacteria www.neogen.com/foodsafetyorder.htm

GENE-TRAK®
Listeria Assay

Results

Positive Negative Positive Negative


Control Control Sample Sample

Product Specifications
Sensitivity: 1-5 CFU/25g
Testing time: 2 hours (after 40-48 hour enrichment)
Tests per kit: Up to 98
Approvals: AOAC Official Method #993.09
Intended Use
GENE-TRAK Listeria Assay provides for the detection of Listeria spp. Materials Required But Not Provided
in food and environmental samples.
Available from Neogen*
1. GENE-TRAK photometer set
The Test
2. Test tube racks
The DNA hybridization test employs Listeria-specific DNA probes and 3. Dipstick holders
a colorimetric detection system for the detection of Listeria species in 4. Wash basins with lids
food and environmental samples following broth culture enrichment. 5. Dilution chart
A sample is considered negative for the presence of Listeria if its absor- 6. Pipettes (1 mL and 10 mL)
bance value is less than or equal to the established cutoff value for the 7. Incubator at 35°C
assay. A sample is considered presumptively positive for the presence 8. 12 x 75 mm glass test tubes
of Listeria if its absorbance value is greater than the established cutoff 9. Minute timer
value of the assay. 10. Micropipette and tips, 100 – 1000 µL range (optional)
11. Modified LCA
Test Procedure
1. Enrich food samples. Not available from Neogen
2. Add 0.5 mL of enriched samples, negative control and positive 1. Blender or homogenizer
control into separate 12 X 75 mm glass tubes. 2. Culture bottles for sample enrichments
3. Add 0.1 mL pretreatment reagent and incubate at 37°C for 15 3. Phosphate-buffered Listeria enrichment broth
minutes. 4. Phosphate-buffered saline
4. Add 0.1 mL lysis reagent to each sample tube. Shake for 5 seconds 5. UVM-1 broth
and incubate at room temperature for 15 minutes. 6. UVM-2 broth
5. Add 0.2 mL Listeria probe solution to each tube. 7. Modified UVM-2 broth
6. Place dipsticks into sample tubes. Raise and lower dipsticks 8. Sterile cotton swabs
5 times to mix and incubate at 65°C for 1 hour. 9. Water baths capable of maintaining 37°C ± 1° and 65°C ± 1°
7. Wash dipsticks two times for 1 minute first at 65°C, then at room 10. Graduated cylinder
temperature. 11. Absorbent paper
8. Transfer the dipsticks to tubes containing 0.75 mL of enzyme 12. Sterile tubes with caps for 1 mL volume
conjugate and incubate at room temperature for 20 minutes. * See Equipment and Accessories, pages 91–95
9. Wash dipsticks two times for 1 minute each at room temperature.
10. Transfer dipsticks to tubes containing 0.75 mL of substrate-
chromogen and incubate at room temperature for 30 minutes.
11. Remove dipsticks and add 0.25 mL stop solution.
12. Read results using a GENE-TRAK photometer.
See package insert for complete instructions
Ordering Information
Prod.# Product description
6705 GENE-TRAK Listeria Assay - up to 98 tests

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