You are on page 1of 1

Food Safety Online Ordering

Foodborne Bacteria www.neogen.com/foodsafetyorder.htm

Reveal®
For E. coli O157:H7
Results

Positive

Negative

Product Specifications
Sensitivity: 1 CFU/25 grams
Testing time: 15 minutes (after 8/20-hour enrichment)
Tests per kit: 20
Intended Use Approvals: AOAC Official Method
No. 2000.13 (8-hour enrichment)
The Reveal for E. coli O157:H7 test system provides for the rapid No. 2000.14 (20-hour enrichment)
recovery of Escherichia coli O157:H7 in foods, allowing detection and
presumptive identification of the organism in as little as 8 hours. Materials Required But Not Provided
The Test Available from Neogen*
1. Stomacher®-type bags
To perform the test, a portion (120 µL) of the enrichment culture 2. Weigh scale
is placed into the sample port of the test device. The sample flows 3. Timer
through the lateral flow device, providing distinct, visible results. If
a line develops only in the control zone, the sample is negative for For 8-Hour Test System
E. coli O157:H7. If lines are present in the control and test zones, the 1. 13 x 100 mm polypropylene or glass tubes
sample is presumptively positive for E. coli O157:H7. 2. Incubator capable of maintaining 42 ± 1°C
3. Disposable sterile 5 mL pipettes
8-Hour Test Procedure 4. Water bath or equivalent heating source
1. Rehydrate enrichment media.
2. Add sample. Incubate at 42±1°C for 8 hours. For 20-Hour Test System
3. Remove liquid sample from the bag following enrichment. 1. Incubator capable of maintaining 36 ± 1°C
4. Heat kill sample. Cool to room temperature. 2. Novobiocin (optional)
5. Place 5 free falling drops (120 µL) in the device’s sample port. Not Available from Neogen
6. After 15 minutes, read and record results. 1. Sterile water
20-Hour Test Procedure * See Equipment and Accessories, pages 91–95
1. Rehydrate enrichment media.
2. Add sample. Incubate at 36±1°C for 20 hours. Ordering Information
3. Place 5 free falling drops (120 µL) in the device’s sample port. Prod.# Product description
4. After 15 minutes, read and record results. 9750 Reveal for E. coli O157:H7 Eight Hour Complete System –
contains 20 devices and 20 media bottles for 20 tests.
See package insert for complete instructions 9751 Reveal for E. coli O157:H7 20-Hour Complete System –
contains 20 devices and 20 media bottles for 20 tests.
9755 Reveal for E. coli O157:H7 – contains 20 devices.
9754 Reveal for E. coli O157:H7 multipack - contains two 10
packs of devices.
9760 Reveal Media – Eight Hour – contains 20 media bottles.
9765 Reveal Bulk Media – Eight Hour – contains 500 g for 56
tests/samples.
9770 Reveal Media – 20 Hour – contains 20 media bottles.
9758 Reveal Bulk Media – 20 Hour – contains 500 g for 60 tests/ samples.
9590 Reveal® AccuScan™ Reader
7985 Novobiocin Supplement, 10 mL - 10 vials/package
To use media other than those listed, see our Acumedia product line.

36 Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com