(2008 Pattern)

M. Sc. Part I – w. e. f. June 2008 M. Sc. Part II – w. e. f. June 2009


M. Sc. (Microbiology) Curriculum
Semester I: Theory Course I Theory Course II Theory Course III Practical Course I Practical Course II - MB-501 - MB-502 - MB-503 - MB-511 - MB-512 Microbial Diversity and Taxonomy Quantitative Biology Cell Organization and Biochemistry Microbial Diversity and Systematics Cell Biology and Biochemistry

Semester II: Theory Course I Theory Course II Theory Course III Practical Course I Practical Course II

- MB-601 - MB-602 - MB-603 - MB-611 - MB-612

Instrumentation and Molecular Biophysics Evolution, Ecology and Environmental Microbiology Microbial Metabolism Ecology and Environmental Microbiology Enzymology and Microbial Metabolism

Semester III: Theory Course I Theory Course II Theory Course III Practical Course I Practical Course II

- MB-701 - MB-702 - MB-703 - MB-711 - MB-712

Immunology Molecular Biology I Virology Microbial Technology Molecular Biology and Immunology

Semester IV: Theory Course I Theory Course II Theory Course III Practical Course I Practical Course II

- MB-801 - MB-802 - MB-803 - MB-811 - MB-812

Pharmaceutical and Medical Microbiology Molecular Biology II Microbial Technology Research Methodology I (Dissertation) Research Methodology II (Dissertation)


1. M. Sc. Microbiology (non-credit system) is a two year postgraduate course, comprising four semesters. At each semester there will be five courses of which 3 are theory courses and 2 laboratory (practical) courses. 2. Eligibility: B. Sc. with Principle subject Microbiology. The concerned centers may conduct their own entrance examination, for admission. 3. Medium of instruction – English 4. Distribution of University and Departmental Courses:

University Courses
Semester - I Semester - II Semester – III Semester - IV MB – 501, 502, 503 MB – 511, 512 MB – 601, 602, 603 MB – 611, 612 MB – 701, 702, 703 MB – 801, 802, 803

Departmental Courses ----MB – 711, 712 MB – 811, 812 (Dissertation)

5. Practical for course no. MB-711 and MB-712 will be conducted throughout the academic year for M. Sc part–II students, while students can carry out the dissertation (practical course MB-811 and MB-812) work throughout the year. 6. Workload: The contact period for each semester will be 12 weeks, 4 lectures per course per week, each of 60 minutes duration. Work load for theory courses of college teacher entrusted with work of post-graduate teaching will be at the rate of one clock hour of post-graduate teaching equal to two periods of undergraduate teaching. Each laboratory course will occupy six hours / week / batch. The work-load for seminars / oral presentations of students will be 4 periods per week per class (M. Sc Part–I and M. Sc. Part–II). Dissertation will occupy equivalent workload of two laboratory courses i.e. 6 hours a week / practical course / batch. 7. Dissertation will be compulsory to all students. Students will carry out dissertation work individually or in the group of not more than three students. Concerned department shall provide all required infrastructure to carry out dissertation work. The format for dissertation report will be similar to the research thesis style; incorporating chapters on: Introduction, Materials and Methods, Results and Discussion and References / Bibliography. The dissertation will be submitted in a typewritten and bound form. Copy of each dissertation will be submitted to the respective department and the center will store it permanently. 8. Review writing and Seminars: Every student will write a review article every semester, based on original and recently published research papers. Every student will give one oral presentation each semester, which will be evaluated by the faculty. Marks for review and the oral presentation will be suitably incorporated in the internal assessment of the practical courses.


9. Examination will be held at the end of each semester. Each course carries 100 marks, the distribution of which is 80 marks for external examination and 20 marks for internal examination. All theory courses are University courses, the examination (external examination) will be for 80 marks and for duration of 3 hours. 10. Examination of practical courses of Semester–I (MB-511 and MB–512) and practical courses of Semester-II (MB-611 and MB–612) shall be examined at the end of respective Semesters. The practical examination for Semester–I will be after the theory examination. These four practical courses will be treated as University courses and will be examined by the examiners appointed by University of Pune, Pune. The practical examination will be conducted for 3 consecutive days, for minimum 6 hours / day / batch. Practical examination of departmental courses will be conducted by respective centers either by continuous evaluation system or on similar lines as university courses. Practical courses of Semester–III (MB-711 and MB–712) and practical courses of Semester-IV (MB–811 and MB–812 - Dissertation) shall be examined at the end of academic year i.e. in the month of April / May of the respective academic year. These four Practical courses will be treated as Departmental courses and examination will be conducted by the concerned department. 11. The examination for internal marks will be conducted by respective departments throughout the semester. 12. Dissertation evaluation: Student/s will submit one copy of the dissertation report to the department. If there is more than one student carrying out a single dissertation, a single report can be submitted and these students will be assessed based on single oral presentation. In such case, presentation should be carried out by all the students carrying out the same work; dividing the presentation equally among them. Evaluation of dissertation will be carried out by two examiners appointed by the concerned department, one of which will be the project guide / supervisor. Student/s will make a oral presentation of the work to an audience comprising of examiners, departmental teaching staff and the postgraduate students of the department. Oral presentation can be carried out using posters, blackboard, transparencies or LCD projector. The allotted time for each oral presentation (one project) should be 10 to 12 minutes, followed by question-answer session of 5 to 8 minutes. The audience can participate in this session The suggested guidelines for evaluation of dissertation work are: Intellectual potential (Understanding of the research problem by the student), Research aptitude (Depth of literature survey for the proposed work, Inputs of student in development of plans and protocols for the experimentation, Ability to analyze data and formulate a solution, Analytical and reasoning abilities of the student for interpretation of data, inputs in discussion), Motivation (punctuality, meeting deadlines and seriousness), Ability to work with others, Maturity of scientific thoughts, Communication skill (oral and written), Proficiency of presentation skills (use of audio-visual aids, preparation of graphs, charts, models, etc., use of scientific language, etc.), Research potential of the work (results and interpretation, outcome of the study and possible future plans, publication potential of the work), The dissertation report preparation (scientific writing) and its contents, Satisfactory responses to the queries from the audience during open defense, etc.


ATKT and marks for passing. etc. but not exceeding 50 % of maximum marks. 5 . Question paper for each theory course will include at least one compulsory problem-question based on research reports (Mathematical / Data Interpretation / Comment type) related to concerned course.13. 14. Standard of Passing: The award of class / grades. Essay type questions should not be asked. Examination for theory examination: Question paper will comprise of 5 questions. will be as per the University of Pune rules. 16 marks each. Number of questions on the topic should reflect in the weightage of the topic in the syllabus. Sufficient option of questions should be allowed in the question papers.

Biochemistry and cell biology Theory Course I MB-501 Theory Course II MB-502 Theory Course III MB-503 Practical Course I MB-511 Microbial Diversity and Taxonomy Quantitative Biology Cell Organization and Biochemistry Microbial Diversity and Systematics Practical course IMB-512 Practical Course II MB-512 Cell Biology and Biochemistry M. Part I Semester – II Theory course I – MB-601 Theory course I – MB-602 Theory course I – MB-603 Practical course IMB-611 Virology Semester III Theory Course III MB-703 Theory Course II MB-602 Theory Course III MB-603 Practical Course I MB-611 Virology Practical course IMB-612 Evolution. Environmental Microbiology and Environmental toxicology Microbial Metabolism. Ecology and Environmental Microbiology Microbial Metabolism Ecology and Environmental Microbiology Practical Course II MB-612 Enzymology and Microbial Metabolism 6 . Tissue culture and Virology Evolution. Part – I Semester – I Theory course I – MB-501 Theory course I – MB-502 Theory course I – MB-503 Practical course IMB-511 Microbial Diversity and taxonomy Quantitative Biology Organization of living systems Microbial diversity. 2005) and New syllabus (implemented from June. 2008) Equivalence to OLD SYLLABUS (June 2005) Course Number Title NEW SYLLABUS (June 2008) Course Number Title M. Sc. Ecology and Environmental Microbiology Microbial Metabolism Ecology. Sc. Systematics and Scientific communication Essential laboratory techniques.The equivalence of the previous syllabus (2005 pattern) with this syllabus (2008 pattern) is as follows: M. Microbiology Syllabus Equivalence Old syllabus (implemented from June. Sc.

7 . Instrumentation and Bioinformatics Immunology. Part II Semester IV Theory course I MB-801 Theory course II – MB-802 Theory course IIIMB-803 Practical course MB-811 Practical course MB-812 Applied Microbial Biotechnology Pharmaceutical Microbiology Molecular Biology II Dissertation. Biophysics and Instrumentation Molecular Biology. Sc. Part II Semester III Theory course I – MB-701 Theory course IIMB-702 Theory course IIMB-703 Practical course MB-711 Practical course MB-712 Immunology.Equivalence to OLD SYLLABUS (June 2005) Course Number Title NEW SYLLABUS (June 2008) Course Number Title M. Molecular Biology I Biophysics. Sc. Bioinformatics and Applied Theory Course I MB-701 Theory Course II MB-702 Semester II Theory Course I MB-601 Practical course MB-711 Practical course MB-712 Immunology Molecular Biology I Instrumentation and Molecular Biophysics Microbial Technology Molecular Biology and Immunology M. Theory Course IIIMB-803 Theory Course IMB-801 Theory Course IIMB-802 Practical Course I MB-811 Practical Course II MB-812 Microbial Technology Pharmaceutical and Medical Microbiology Molecular Biology II Research Methodology I (Dissertation) Research Methodology II (Dissertation) Dissertation.

the guidelines from CSIR and UGC curricula for research fellowship were taken into account. planning and policy making. This seems to be unjust for the students as well as examiners. Teachers and Heads of the Postgraduate Department / Centers should take equal interest in these dissertations and should stay in communication with the research guide for the dissertation regarding progress of work. These laboratories accept only a limited number of students. the dissertations were evaluated by University appointed examiners and only two examiners (Internal and External) evaluated all the dissertations from diverse research areas (even though. Syllabus reforms involved discussions with experts from educational institutes. Teaching at undergraduate / postgraduate level courses in Microbiology / Biotechnology To keep the syllabus relevant to national needs. that after their postgraduate studies. both in research and industry. 3. 2. combined with acquaintance with front-line research trends and developments in related field is the aim of postgraduate curricula. Teachers should also see that the results of dissertation do not go waste and attempt to publish it in the form of a scientific publication. Dissertation at M. As per earlier syllabus structure. Sc. but all the researchers necessarily come from postgraduates in the subject and therefore postgraduate curriculum in all its different aspects such as design. The curriculum should be designed in such a way. A decision was taken that the dissertation work (in lieu of practical course) will be treated as departmental course. Research at national. The expectations and opportunities available for Masters Students are responsible positions in technical production. 2008) The objectives of postgraduate training are orienting the students for research towards higher degrees. Imparting in-depth fundamental knowledge of the diverse areas of the discipline. With increasing number of students every year it has become difficult for students to find placement to do research project for dissertation. thus allowing for flexibility and freedom for concerned centers to appoint examiners with expertise and research experience / interests in the topic of research work carried out by the candidates. 8 . Planning and policy making for biotechnology 4. they may not have had the expertise in all areas!). Part II: The suggestions received from Peers regarding postgraduate dissertations state that: 1. Microbiology curriculum (Implemented from June. After completing this curriculum the students should be able to take up the following responsibilities: 1. instead of University course. Though all postgraduate students do not undertake research career. or in the field of development of industrial processes. Research laboratories are finding it difficult to provide facilities for number of students.Salient Features of M. 2. implementation and evaluation needs necessarily to be research oriented. Higher positions in biotech production units 3. students should be able to work directly in the applied field (industry or research institute) without any additional special training. international level. Sc. research and industry as well as few past and present students.

food. vaccine developments. we feel certain topics in the present syllabus need to be supplemented and strengthened by inclusion of few additional topics.) and biotechnological industries. The Board of Studies in Microbiology has identified the following thrust areas and prospective plans for syllabi reforms at postgraduate level: • Microbial Technology – includes application of bacteria. wine. fungi. evolution. yeast genetics • Determinants of microbial pathogenecity • Immunopathology. To enrich students’ knowledge and train them in the above mentioned areas. Modern biology combines the principles of chemistry and biological sciences (molecular and cellular biology. developmental biology. a recombinant or a genetically manipulated organism (GMO). Microbiology and Biotechnology deal with overlapping interests. • Environment – includes cleaner processes that produce less waste and use less energy and water in such industrial sectors as chemicals. remains the same i. micro-organisms from extreme environments. most of the science students aim at professional courses. Comparatively less number of students opts for degrees in Biosciences. identification of bacteria • Possible utilization of microbial population from extreme environments 9 . Areas that need to be introduced in syllabi have been identified as: • Eukaryotic cellular organization • Eukaryotic gene expression e. pulp and paper. energy. Both these disciplines viz. dairy. and for last 2 to 3 years it has shifted partly to Biotechnology courses. therapeutics and pharmaceutical approach towards diseases. epidemiological characterization of diseases. exploration and utilization of diversity to identify and harvest biomolecules for human health improvisation. • Human health – includes pathogenic micro-organisms (bacterial. genetics. For several years now. etc. ecology and geomicrobiology. immunopharmacology and cancer biology • Protein stability. conformation and folding • Over-expression of recombinant proteins • Biocontrol • Bioinformatics • Molecular tools for characterization. the first preference of students desiring to enter the field of Life Sciences has been Microbiology. stem cell research. and metals and minerals. etc. antibiotics. bioremediation and bioprospecting • Microbial diversity – includes collecting information of diversity.e. computer science) to produce goods and services and for environmental management. • Research in life-sciences – includes research tools like immunology and molecular biology. protozoan and fungal).g. fermentation. however. textiles and dyes. and immunology) with technological disciplines (engineering. etc. Tools of molecular biology play an important role in preparation of an engineered clone.Overall picture of student trends (before undergraduate studies) in selecting courses is very typical. • Agriculture – includes biofertilizers and biocontrol. etc. viral. gene therapy. protozoa and viruses in traditional (food. harnessing microbial utilities avoiding the use of caustic chemicals. Microbial sciences focus more on study of the microbial world (this limitation needs to be corrected!) while Biotechnology focuses more on application of mammalian systems. The main theme of teaching these courses. application of basic principles of Life Science to develop into technology. Archeabacteria. diagnostics. particularly leading to studies in Engineering.

molecular biology.MB-601 . analytical and quantitative instrumentation and biophysics.MB-711 .MB-511 . tissue culture. The skill sets thus evolved will help the students in academic and applied research.MB-612 Instrumentation and Molecular Biophysics Evolution.MB-701 . the methodology to study life processes will be introduced. The biophysical studies have lot of potential in genomics. Microbiology syllabus is as follows: Semester I: Theory Course I Theory Course II Theory Course III Practical Course I Practical Course II . Semester III: Theory Course I Theory Course II Theory Course III Practical Course I Practical Course II . students have now developed knowledge base in the life science fundamentals and will be ready to study different tools like immunology and molecular biology. in general.MB-703 . The compartmentalization of pure sciences will now start disappearing and students will be ready to undertake studies in life sciences.MB-712 Immunology Molecular Biology I Virology Microbial Technology Molecular Biology and Immunology At the end of first part of postgraduate studies. The laboratory work in these areas will help the students to develop practical applications in these areas. since prokaryotic especially bacterial cell structure was dealt with in under-graduation. proteomics and drug discovery.MB-512 Microbial Diversity and Taxonomy Quantitative Biology Cell Organization and Biochemistry Microbial Diversity and Systematics Cell Biology and Biochemistry The theory and practical courses will encompass the microbial world – its extent and spread with examples. Sc.MB-702 . biophysics. At this stage some theoretical biology aspects (evolution) and applied aspects (environmental) have also been introduced.MB-501 . development of research ideas and planning / designing of research projects. Quantitative biology is supportive to these aspects and also prepares the students for data handling and processing strategies that is an integral part of research and applied science. instrumentation. Ecology and Environmental Microbiology Microbial Metabolism Ecology and Environmental Microbiology Enzymology and Microbial Metabolism Once the knowledge base of microbial world is build up. Semester II: Theory Course I Theory Course II Theory Course III Practical Course I Practical Course II .MB-611 . The course contents deal with cellular organization focusing on eukaryotic cell.MB-503 .In addition. the structure of the M. The virology is such an area that deals with different aspects of life. with a 10 . data handling and processing. Thus.MB-603 . acquaint the students with traditional and biochemical as well as molecular tools and bioinformatics tools to study the microbial diversity. we feel that the students should be well acquainted with research methodology which includes different skill developments in scientific writing.MB-502 . like biochemistry.MB-602 . etc. This includes metabolism. Enzymology.

Sc. we would like to assure that given time. researchers and industry. Sub-committee for M. Sc. the dissertation work will now be spread over two semesters. Board of Studies in Microbiology December. the Microbiology faculty is capable of adjusting to these changes. All three theory papers will deal with basic concepts as well as research. evolution. since the teachers will have to upgrade their knowledge and skills in these areas which is a slow process. Students are also required to write a review of literature on a specific topic dealing with frontline research at M. providing sufficient time for undertaking thorough study and execution of a research topic. scientific writing. genomics and applied approaches. With the theoretical and practical training in biostatistical tools. execution. However. data analysis and interpretation.MB-812 Pharmaceutical and Medical Microbiology Molecular Biology II Microbial Technology Research Methodology – I (Dissertation) Research Methodology – II (Dissertation) The last semester of deals entirely with applied approach in different areas of life sciences with specific examples from microbial sciences. biophysics and students will be ready to tackle this challenging field. With the change in structure of departmental and university courses. Sc. We submit here that total change in course content is not possible. This should also help to develop fruitful interactions between academicians. presentation – oral and in writing. students will be capable of carrying out quality research for their dissertation work.specific application as tool in cell biology. every semester.MB-801 . The drug discovery and development can be carried out using traditional methods as well as modern tools like molecular biology. The M.MB-802 .MB-803 . collection of background information and tracking the developments in a particular research topic. Members. Semester IV: Theory Course I Theory Course II Theory Course III Practical Course I Practical Course II . The medical microbiology is presently hot field for research in India and abroad and presently there is dearth of expertise. We are confident that postgraduate microbiology degree holder will provide the required theoretical and laboratory expertise needed by biotech industry and life-science research institutes. proteomics. 11 . seminars and review writing. planning experimentation. The experimentation part in theory and practical will be helping the students to develop skillsets necessary for work in any specific areas of life science and biotechnology. Microbiology students are required to make a presentation of a published research paper in the form of seminar. Research aptitude development: The students are trained in different areas that are required to develop research aptitude viz.MB-811 . Microbiology Syllabus Members. benefiting the faculty and ultimately the students. In some areas. part I and part II. the expertise may not be presently available and collaboration with industry and research institutions is necessary. 2008.

Genome Biology.) and Fungi: 1. A. North Holland Publishing Co. References: Taxonomy 1. local and global alignment. 5. dynamic methods. 12 . 2. (1974). 1 – 5) (2001 – 2003). Methods of extracting total bacterial DNA from a habitat.8. L. Methods in Taxonomy of Bacteria (including archaebacteria. Academic Press. BLAST and FASTA versions. 3. (2002) Exploring Prokaryotic Diversity in the Genomic Era. Amsterdam. Illustrated Genera of Imperfect Fungi. Actinomycetales: Characteristics and Practical Importance. 1973. Bergey’s Manual of Determinative Bacteriology. Hugenholtz P. Breed and Buchanan. 0003. 143-169. Sykes. 1974. Morphological Methods 2. (2002) Microbial Biodiversity: Saving Bacteria to save ourselves. scoring matrices. Methodology of rRNA sequencing (teach as per practical / experimental methods) 2. Phylogenetic Identification and In situ detection of Individual Microbial Cells Without Cultivation. Minnesota. Amann R. Barnett. Genetic Methods 4. Breed and Buchanan. measures and indices of diversity. B. 1982. B. Bergey’s Manual of Systematic Bacteriology. estimates of total number of species. and Schleifer K. Society for Applied Bacteriology Symposium Series No. Bioinformatics (10) Sequence alignment. 6. Lodder J. The Yeasts: A Taxonomic Study. 3(2). 9th Edition. Skinner (Eds). C. (1995). Diversity (16) The expanse of microbial diversity. Microbiological Reviews 59. Harvard Science Review. 4. database search for homologous sequences. Cook T. Ludwig W. Diversity 1. Bergey’s Manual of Determinative Bacteriology. Smith-Waterman algorithm. and F. 8th Edition. (Volumes. H. Newer approaches for exploring unculturable bacteria: Culture independent molecular methods. 2. G. 26-28. Methodology of identification of unknown pure cultures: Strategy and methods B.SEMESTER – I Theory Course I – MB-501: Microbial Diversity and Taxonomy A. Breed and Buchanan. 2nd Edition. Chemotaxonomy 3. Burgess Publishing Co.1-0003. 2. and Hunter. Taxonomy (22) 1. 3. 1960.. Needleman and Wunsch algorithm.

New Jersey. Zoe L. and Ouellette. and Gregory R. 4. Woese C. (1987). Cold Spring Harbor Laboratory Press. 221-271. 141-150. 6. T. 13 . Grant. S. Nature Reviews 2. (Eds. W. Morgan Kaufmann Publishers. Volume 132. Baxevanis. Cambridge. (2001) Bioinformatics: The machine learning approach. John Wiley & Sons. (Eds. New York. and Krawetz. Bioinformatics: Methods and Protocols. Keller M. Springer. Methods in Molecular Biology. 3. Microbiological Reviews. An Introduction. Lacroix. Mount. (2004) Statistical Methods in Bioinformatics. New Delhi. P. 2000. Ewens Warren J. S. Managing Scientific Data. and Zengler K. 734-740. New York. New York. Bioinformatics. F. D. Second Edition.4. F. 2. A. MIT Press. A.) 2003. B. (2001) Bioinformatics: sequence and genome analysis. and Critchlow. (1997) A Molecular View of Microbial Diversity and the Biosphere. 7. (2004) Bioinformatics: Managing Scientific Data. Bacterial Evolution. 5. S. Bradford Book.). Bioinformatics 1. (2004) Tapping in to Microbial Diversity. Misener. 5. (2001) Bioinformatics: A practical guide to the analysis of genes and proteins. 6. Pace N. 276. D. Z. Science. Humana Press. Morgan Kaufmann Publishers. Baldi. and Brunak. and Terence C.

Models in population genetics. Descriptive statistics: Frequency and probability distributions. tabulation. Hypothesis testing: comparison of two sample means: t-tests. Rothery (1993) Models in Ecology. Feller W. 4. b. data organization. Kluwer Academic Publications 7. independence and randomness g. kurtosis. 6. 14 . Survey design e. Gupta and Dasgupta – Fundamentals of statistics. skew ness. Factorial design. (1996) Modeling Biological Systems : Principles and Applications. Haefner James W.. Ukaaz Publications. Epidemiological models 2. non-parametric tests. Binomial and Poisson distributions and their applications. examples and problems for each of the following: a. c. Computer simulation of biological systems. World Press . Cochran W. 2nd Ed. significance level. & P. Concept and applications of modeling: (10) a. Goon. Concept and applications of databases b. References: 1. writing simple simulation programs for growth models. Concept and applications of internet c. Introduction to the concept of stochastic models.G. Population models: Exponential. d. Irfan Ali Khan and Atiya Khanum. 3. B) Modeling in Biology 1. ANOVA and F test.Statistical methods. curve fitting and choice of models. measures of dispersion. Fundamentals of Biostatistics. f. logistic and chemostat models. h. Introduction to Normal. Probability: Laws of probability. 5. models based on Hardy-Weinberg equation. measures of central tendency. population interactions and pathway regulation. New Delhi. b. standard error and confidence interval. Introduction to multivariate analysis: multiple regressions. – Introduction to probability theory and its applications. Use of Computers in Biology (10) a. sampling methods. Distribution of sample means. ordination. The concepts of null hypothesis. Sultanchand & Sons. graphical representation of distributions. type I and type II errors. one tailed and two tailed tests. Wiley 2. Mumbai. Categorical data and proportion data: Chi square test and test for goodness of fit. – Sampling Techniques. graphical representation Concepts. Regression and correlation.P.SEMESTER – I Theory Course II – MB – 502: Quantitative Biology A) Biostatistics (28) Quantitative methods in biology. scales and variables. Asia Publishing House. principal component analysis. Hydrabad. Kolkata. Brown D. Gupta S.Wiley eastern Ltd. d. c.

Statistical Theory.N. – Sampling methods. (1990) Innumeracy: Mathematical Illiteracy and its consequences. Edition 7. McGraw Hill Publishing Co. 10... 13. Kolkata. Graybill F. 12.M.C. John Wiley & Sons. Wayne Daniel (2007) Biostatistics A foundation for Analysis in the health sciences. John Maynard Smith (1974) Models in Ecology. Macmillan Publishing Co. Paulos John A..India edition. Montgomery D. Vintage Books Paperback. 14.W. 15 . Mood A. Inc. and Bose D. Wiley. Indian Statistical Institute. 11. 9. Murthy M. Cambridge University Press.Introduction to the theory of statistics.C.8. – Design and analysis of experiments. Lindgren B.

oligosaccharides and polysaccharides. deoxy sugars. tautomeric forms of bases and their implication in pairing of bases. Van der Wall’s interaction. saturated. detection and estimation of lipids Vitamins: (2) Structure and function of fat soluble vitamins as vitamins A. B and Z forms). unsaturated. structure of DNA (A. E and K C) Ultrastructure and Organization of Eukaryotic Cell (12) Structural organization of: Cytoskeleton (structural proteins – microfilaments. nucleus. structure and biological activity f. sphingolipids. Host-guest interactions c. terpenes. steroids. asymmetric centre in sugars. fatty acids. etc. branched. 16 . D-series. primary. C-terminal determination. r-RNA. tertiary. with examples. system structure and function of triglycerides. charge transfer complexes. sugar epimers. structure of t-RNA. nomenclature. Tm value. Stereochemistry: Three dimensional shape of molecules. estimation of nucleic acids Lipid Chemistry: (3) Classification of lipids according to chemical structure. Chemical reactivity: Concept and factors affecting reactivity (Inductive effect. sugar derivatives such as sugar alcohols. determination of primary structure of polypeptide (N-terminal. Substitution. nucleosides. ionic bonding. secondary. Concept of pH of weak acids and weak bases. leavo-rotatory. d. 5’ phosphate.). protein detection and estimation. Carbohydrate Chemistry: (3) Mono. etc. classification of amino acids. di. partial double bond nature of peptides. chemical reactions of amino acids. phospho-diester linkages. dextro.g. Bioorganic mechanism of enzyme catalyzed reactions: stereochemical aspect inhibition by penicillin e. etc. Addition. 3’hydroxyl polarity of nucleic acids. amino sugars. prostaglandins. D. estimation of carbohydrates Nucleic acid Chemistry: (3) Structure of bases. Elimination. peptide linkage. Bonding other than covalent – H-bonds. reducing and non-reducing sugars. Tantomerism. Ion-dipole. strength of buffer. Henderson-Hasselbalch equation. actins. Oxidation. conformation and configuration. amino acids as buffers. Reduction. nucleotides.SEMESTER – I Theory Course III – MB – 503: Cell Organization and Biochemistry A) Introduction to Bioorganic Chemistry (10) a. and m-RNA. Reactions of organic molecules: A brief overview of important reactions in organic chemistry e. sugar acids. quaternary structures of proteins. concept of buffer. Resonance / Mesomeric effect. Rearrangement. waxes. phospholipids. Conjugation and Hyper-conjugation. sugar anomers. important biological buffers (with the help of numerical problems) B) Chemical Composition of Living Systems Protein Chemistry: (3) Structural features of amino acids. Mitochondria and chloroplasts and their genetic organization. L-series.) b. buffer value. structural classification of proteins. method of sequencing of peptides).

Doi Roy H. Wolpert Lewis. 2. London. Methods in Molecular Biology. antero-posterior and dorsoventral polarity E) Communication And Coordination (6) Cell signaling and communication in Dictyostlium. Paddock. Gibert Scott F. John Wieley and Sons. Ingraham J. Confocal Microscopy. CUP. Edimberg.. Confocal microscopy D) Development And Differentiation (6) Introduction to Developmental Biology. cell proliferation and cell death. Edition 7. re-differentiation and trans-differentiation. W. Protein trafficking. Stanier R. Developmental Biology. 3. (1972). (1987) Outlines of Biochemistry Edition . Alberts Bruce (1985) Molecular Biology of Cell.. Bruuening George. Advanced Organic Chemistry. New Delhi. Golgi apparatus. Mac Millan Press. Press Inc. NJ Development and Differentiation 1. Principles of Development. Berg Jeremy. (2003). 4th edition. Stryer Lubert (2001) Biochemistry. Jerry March. dedifferentiation. Organic Chemistry. Oxford University Press. Stumpf Paul K.. Oxford. Inc. Stumpf Paul K. teratogenesis. 3. Freeman. F.. Tymoczko John. Cellular and Molecular Biology Lea and Febiger. A. New York. Conn Eric. (1987) Outlines of Biochemistry. 2. New York. D. 122. P. Concepts of commitment. morphogen gradients in developmental regulation. from Methods and Protocols Vol. Totowa. 5. Oxford Press 2. 17 . Myxobacteria. (1997) Developmental Biology. Warren and Wothers. Immuno-electron microscopy. Cambridge. Y. (1995) General Microbiology. Conn Eric. Regulation of cell cycle. 3. Muller W. L. organizer and its importance giving examples of invertebrates (Drosophilla) and vertebrate (Xenopus) model systems. Philadelphia. Events in cell cycle. Schlegel Hans G. Humana Press. Hox code.Endoplasmic Reticulum.. Localization of macromolecules using electron microscopy.A. John Wiley Ultrastructure and Organization of Eukaryotic Cell 1. pattern formation in body axis. Doi Roy H. John Wieley and Sons. De Robertis E. 4. and De Robertis E. (1998).. Chemical Composition of Living Systems 1. quorum sensing. Conserved nature of development. gastrulation and cellular movements involved in it. (1987). Churchill Livingston. Dawes Edwin A. New Delhi. 7th Ed. Garland Pub. Importance of its regulation. determination and differentiation. Stephen W. apoptosis. Bruuening George.. H. Clayden.. Springler – Verlag. (1976) General Microbiology. 2. MPF. 6th Edition. homeostasis. Biofilms and their application References: Introduction to Bioorganic Chemistry 1. 6. 5th Edition . Quantitative Problems in Biochemistry. M. Adelberg E. Greeves. USA. Sinauer Associates Inc. Mass.

Petersm J. Volume 1 & 2. Biochemical Calculations. Kaplan. Bacteria. Oxford University Press. (Eds. Blackie. London. 185-210. Academic Press California. H. (1980). Ribbons) Vol. and Cox M. M. Vol. Laskin A. and Lechevalier H. CRC Handbook of Microbiology. Communication and Coordination: 1. 2nd Ed. (1969) Isolation. T. Smith Emil. 2nd Ed. 10. Hamilton W. 2. 8. White David (2000) Physiology and Biochemistry of Prokaryotes. Segel Irvin H. in Ecology of Microbial Communities. L. Toole ‘O’ George. (1983) Principles of Biochemistry. New York. and W. (1997). (1977). Peberdy John F. Norris J. E. Handler Philip. (2001) Biochemistry: The Chemical Reactions of Living Cells. 9. New Delhi 7. 1. Mac Millan Worth Pub. Vol. cultivation and maintenance of Myxobacteria. Co. M. CRC Press Ohio. (2002) Lehninger’s Principles of Biochemistry. 5. G. Nelson D. G. 3. R. Allan. Academic Press London. Inc. R. 6. Edition 6. B. Tata Mc-Graw Hill Companies.(2000) Biofilm formation as microbial development Annual Review of Microbiology. White Abraham. Methods in Microbiology (Eds. New York. Kolter. Hill Rober. 54 49-79 18 . (1987) Biofilms: Microbial Interactions and Metabolic activities. Developmental Microbiology. A. 3B. John Wiley and Sons. Cambridge. Gray and J. Fletcher. Jones) Cambridge University Press. Lehman J. I. R. Metzler David E.4.

simulation of population growth in batch and continuous culture 6. Electrophoresis: Agarose gel electrophoresis. acetic acid and sodium acetate. Isolation. maintenance of instruments 2. Isolation and characterization of cyanobacteria 7. disposal of chemicals and cultures. handling of chemicals. Isolation. SEMESTER – I Practical Course II – MB – 512: Cell Biology and Biochemistry 1. Colorimetry and spectrophotometry: Estimation of sugar and total carbohydrate. Sequence matching using BLAST. identification and characterization of molds 4. Isolation. estimation of protein by Lowry. Isolation. K2HPO4 and H3PO4 3. identification and characterization of yeast 3. Preparation of buffers using KH2PO4 and K2HPO4. hazard from chemicals. Statistical analysis using Excel. calibration and validation instruments. Determination of sugar composition (qualitative) in cell walls of actinomycetes 8. Isolation. RDP. Buffer: Determination of pKa of a monoprotic weak organic acid.SEMESTER – I Practical Course I – MB – 511: Microbial Diversity and Systematics 1. purification and estimation of RNA from Yeast c. Isolation and characterization of halophiles (One isolate from all the groups 1 to 7 and identification upto genus level) 8. identification and characterization of actinomycetes 2. Computer applications: Plotting graphs. Isolation and characterization of bacterial pigment 9. Isolation and characterization of thermophilic microorganisms 6. isolation and characterization of PHB granules in bacteria 10. Detection. PAGE and SDS-PAGE of proteins 7. purification and estimation of chromosomal DNA of bacteria b. recording of scientific experiments. Isolation and characterization of anaerobic microorganisms 5. Good laboratory practices: Laboratory safety. Chromatography: Separation of sugar and amino acids by paper and thin layer chromatography 4. preparing / designing SOP for the same. Molecular Taxonomy: a. Bradford and UV Spectrophotometry 5. Standardization of laboratory procedures. Determination of saponification value and iodine number of fat 19 .

Gel filtration chromatography. Spin-spin coupling. Differential centrifugation. Drenth.. J. Tymoczko. ionization. J. 3. Garnier-OsguthorpeRobson (GOR) method. Relaxation parameters. Biological applications. Chromatographic techniques: Basic concepts. Secondary. Combination with chromatographic methods. Infrared spectroscopy. John Wiley & Sons. M. Isoelectric focusing 3. Centrifugation: Basic concepts. Chou-Fasman method. Line width. Protein structure / properties determination: a. Electrophoresis: Basic concepts. Ramachandran plot. Spectroscopy: Basic concepts. MALDI-TOF b. L. X-ray crystallography: Isolation and purification of proteins. R. Liquid scintillation counting. Density gradient centrifugation. Classification of three dimensional structures of proteins (motifs and fold domains) 2. Intensity. Isopycnic centrifugation 4. and Stryer. Berg. 6th Edition. Radiography: Tracer elements in Biology. Nuclear Overhauser Effect. (2007) Principles of protein X-ray crystallography. FTIR 5. Neural networks. super-secondary. denaturing and gradient). Gas chromatography. (2006) Biochemistry. Theoretical and experimental methods for determination of size of proteins. acquisition of the diffraction pattern. Cotterill. Ionexchange chromatography. UV/Visible spectroscopy. Phase determination ii. Physical nature of non-covalent interactions. Germany. J. Fluorescence spectroscopy. L. Affinity chromatography.SEMESTER – II Theory course I – MB – 601: Instrumentation and Molecular Biophysics A) Instrumentation: Principles and applications of: (24) 1. J. Gel Electrophoresis – agarose and acrylamide (native. Radioactive isotopes. Half life of isotopes. 20 . Phosphor imaging B) Molecular Biophysics 1. England. Circular Dichroism (CD) and Optical Rotary Dispersion (ORD). Theoretical methods (Concept and introduction): (4) Lim’s stereochemical method. Pulse chase experiment. Ultra centrifugation. NMR Applications in Biology iii. instrumentation. New York. High Performance Liquid Chromatography 2. Mass spectroscopy: Principles of operation and types of spectrometers. NMR spectroscopy: Basic Principles of NMR. basic principles of x-ray diffraction. Conformational properties of proteins. Chemical shift. Autoradiography. crystallization of proteins. Experimental techniques: (15) i. 2. Homology based methods References: Instrumentation 1. M. Ion fragmentation. 3rd Ed. (2002) Biophysics: An Introduction. Properties of amino acids and peptides: (5) Physical and chemical properties of amino acids. Freeman. Springer. tertiary and quaternary structures of proteins. Čerenkov radiation. Ion transport and ion detection.

N. B. 6th Ed. Germany. Narayanan.4. John Wiley & Sons. Brooks/Cole. Molecular Biophysics & W. 3. England. Second Edition. and Gautham. M. New Age International Publication. Springer. Mount. (2001) Bioinformatics: sequence and genome analysis. (2003) Calculations in molecular biology and biotechnology: A guide to mathematics in the laboratory. (2006) Methods in modern biophysics. London. V. H. R. (2000) Computational Analysis of Biochemical Systems Cambridge University Press. H. Wilson Keith and Walker John (2005) Principles and Techniques of Biochemistry and Molecular Biology. (For numerical problems in instrumentation) 21 . Keeler. New York. J. Pattabhi. 9. (1995) Proteins NMR Spectroscopy: Principles and Practice. New York and Narosa Publishing House. New York. J. Voit E. F. (2004) Biochemistry. O. Academic Press. 5. Academic Press 2. Nölting. 3rd Ed. Nalting B. (2002) Understanding NMR Spectroscopy. P. 7. Cambridge University Press. Cavanagh John et. and Grisham. Publishing Company. 6. Oxford University Press. Nalting (2003) Methods in Modern Biophysics Springer Verlag 4. Elsevier Science. Delhi. W. Duffin (1999) Molecular Biophysics: Structures in Motion. D. & B. (2000) Essentials of Biophysics. 8. California. Cold Spring Harbor Laboratory Press. Daune M. New Delhi 2. Other books: 1. (2002) Biophysics. Garrett. Stephenson. Kluwer Academic Publishers. C.

evolution of rates of mutation. organic matter production (DOM). (competition. benevolent interactions (control within the microbial communities of rhizosphere). rhizosphere environment and recognition phenomenon.) Working treatment systems and their analysis (reactions and kinetics. sexual selection. sociality and multicellularity in microorganisms . lectines. origin and stability of diversity. evolutionary stability of cooperation. functional capability. the origin of new genes and proteins. control and reuse. adsorption. hydraulic retention time. Game theory. siderophore. antibiosis. modeling of growth and distribution of marine micro plankton. Ecology and Environmental Microbiology A) Evolution: (15) History and development of evolutionary theory Neo-Darwinism: Spontaneous mutation controversy. mean cell residence time. octapine. suspended and attached growth processes. diversity of secondary metabolites B) Ecology: (15) 1.) Critical operating parameters like DO. disinfection. selection of reactor type. ageing. 5. evolutionary trade offs. rhizoplane. Effluent disposal. indole acetic acid. mass balance analysis. Wastewater treatment system (unit process): Physical screening. paper and pulp manufacturing industries) 22 . phylogeny and molecular distances. antagonistic interactions. Evolutionary origin of biochemical disorders: The case of insulin resistance. Malfunctioning of treatment systems due to shock loading. Mycorrhiza: Host-fungus specificity. Evolution of life histories. levels of selection. flocculation. interaction of mycorrhizal fungi with non-host plants. predation etc. F/M ratio etc. Regulation and limit for disposals in the lakes. group selection and selfish gene. dechlorination 3. hostparasite co-evolution. Current industrial wastewater treatment and disposal processes ( Textile. 3. reactor types. gas transfer. strategies of organic matter utilization and microbial utilization of organic matter in sea. Sociobiology. Co-evolutionary strategies. and remedial measures adapted. flavonide from plants. 2.).SEMESTER – II Theory course II – MB – 602: Evolution. Molecular evolution: origin of life. flow equalization. Neutral evolution and molecular clocks. C) Wastewater Treatment: (18) 1. Speciation in sexual and asexual organisms. dyestuff. hydraulic loading etc. hydraulic character of reactors. mechanism of dissolved. parasite theory of sex and sexual selection. Marine ecosystem: Environment of marine bacteria. flotation. kin selection. Rhizosphere. granular medium filtration. and land. mixing. types of selection. nipotine. diary. host fungus interactions. 2. Direct and indirect reuse of treated effluents and solid wastes. Community ecology: community structure. bacterial growth in sea and its regulation by environmental conditions. Chemical precipitation. oceans. Water pollution control. Evolution and stability of sex. r and k selection. rivers. 4. Biological: (aerobic and anaerobic.

(1974). Metcalf and Eddy (Eds). (2000). Richards. Wastewater Engineering. 3. New York. Burton. S. Ltd. Strickberger M. New Delhi. Disposal and Reuse. London. (1978). Waste Water Treatment: 1. (1991).. and F. Ecology 1. Ridley Mark (2004). 2. T. Glasgow. Meadows. B. Evolution. Blackie & Son Ltd. (1987). and J. Tata Mac Graw Hill Publishing Co. W.References: Evolution: 1. 23 . An introduction to Marine Science. I. Campbell. T. Longman Group Ltd. Evolution. Tchobanoglous G... L. Freshwater Ecology. 2. Treatment.N. Blackwell Science Ltd. P. Longman Scientific & Technical.. Jones & Bartelette Publications. 3rd Ed. Microbiology of Terrestrial Ecosystems. Macan.

Bacterial photosynthesis: scope. free energy and equilibrium constant. concept of allosterism. electron carriers in photosynthesis. photolysis of water. free energy. Wyamann and Changuax model. Biochemistry of methanogenes F) Nitrogen Metabolism: (10) a. cyclic and non-cyclic flow of electrons. C) Aerobic Respiration: (6) Mitochondrial electron transport chain. inhibitors and un-couplers of electron transport chain and oxidative phosphorylation. B) Enzymes: (10) Purifications of enzyme. F) Membrane Transport: (6) The composition and architecture of membranes. examples of allosteric enzymes and their significance in allosteric regulation. ammonia assimilation with respect to glutamine synthetase. purification chart. light and dark reaction. Hill plot. Ion 24 . Kinetics of reversible inhibitions enzyme catalyzed reactions. determination of feasibility of reactions. Biosynthesis of purine and pyrimidine bases G) Photosynthesis: (6) Energy consideration in photosynthesis. Biosynthesis of five families of amino acids and histidine. glutamate synthetase. Membrane dynamics. positive and negative co-operativity. D) Anaerobic Respiration: (4) Concept of anaerobic respiration. glutamate dehydrogenase. Hill reaction. determination of free energy of hydrolytic and biological oxidation reduction reactions. Solute transport across membranes: Passive diffusion. Biochemistry of biological nitrogen fixation. kinetics of single substrate enzyme catalyzed reaction. generation and maintenance of proton motive force. models of allosteric enzymes (Monod. Z scheme. Photosynthetic reaction center. Nemethy and Filmer model).MB – 603: Microbial metabolism A) Bioenergetics: (6) Laws of thermodynamics. active transport using P and F type ATPases. their properties and regulation b.SEMESTER – II Theory course III . Koshland. oxidized sulfur compounds. cyclic flow of electrons. King Altman approach to derive – two substrate enzyme catalyzed reactions. bacterial photophosphorylation in various groups of phototrophic bacteria. coupled reactions. types of two substrate enzyme catalyzed reactions. enthalpy. Organization of photosystem I and II. oxidative phosphorylation. and nitrate as electron acceptor with respect to electron transport chain and energy generation. properties of nitrogenase and its regulation. kinetics of allosteric enzyme. entropy. structure and function of ATPase (bacterial and mitochondrial). electron donors other than water in anoxygenic photosynthetic bacteria. electron carriers. phosphorylation potential and its significance. Gibbs free energy equation. under standard and non-standard conditions. Energy generation in all groups of chemolithotrophs. high energy compounds. Atkinson’s energy charge.

13. (1987) Outlines of Biochemistry 5th Ed . Lehman J. (1995) Biochemistry. Handler Philip. D. 2. 9. Stryer Lubert (2001) Biochemistry 4th Ed. and Cox M. John Wiley and Sons. White Abraham. 8. K. England. (2005) Lehninger’s Principles of Biochemistry. Tymoczko John. New York. Freeman & Co. W. (1972) Quantitative Problems in Biochemistry. Edinburgh. John Wiley and sons New York. (1988) Microbial Physiology 2nd Ed. 11. Edition 6.mediated transport. 2nd Ed. 4. Brown.. L. Segel Irvin H. (1997) Biochemical Calculations 2nd Ed. 12.. W. Inc. Conn Eric.. Mandelstam Joel and McQuillen Kenneth (1976) Biochemistry of Bacterial Growth. (2001) Biochemistry: The chemical Reactions of Living Cells. 10. Moat Albert G. 14. Smith Emil. John Wiley and Sons New York. Chinchester. Zubay Geoffrey (1998) Biochemistry. John Wiley and Sons. 7. Berg Jeremy. Hall D. 4th Ed. 3. Freeman.. M. Oxford University Press. Churchill Livingston. (1983) Principles of Biochemistry. Voet Donald and Voet Judith G. H. Fourth edition. and Foster John W. New York.. 5. Cambridge University Press. Dawes Edwin A. Bruuening George. Palmer Trevor (2001) Enzymes: Biochemistry. New York. Horwood Pub. Metzler David E. Hill Rober. Co. Biotechnology and Clinical chemistry. Tata Mc-Graw Hill Companies. Liposomes References: 1. Blackwell Scientific Publication London. New Delhi. C. H. transport of ions across membranes (ion pumps). (1996) Photosynthesis 5th Ed.. Volume 1&2. Stumpf Paul K. Academic Press California. Model membranes. and Rao K. 25 . Nelson D. W. White David (2000) Physiology and Biochemistry of Prokaryotes. 2nd Ed. New York. Doi Roy H. 6. New York.

Isolation and characterization of chemolithotrophic microorganisms 10. Estimation of microbial species diversity in microecosystem Effect of stress Temperature. Isolation and characterization of (as nitrogen fixers) of Azospirillum and detection of IAA by Azospirillum 8. nitrate. Detection of siderophore production by Azospirillum and Pseudomonas 9. Detection of Aflatoxin in food / culture SEMESTER – II Practical course II – MB – 612: Enzymology and Microbial Metabolism 1. salt concentration. Determination of Km and Vm values of Amylase 6.SEMESTER – II Practical course I – MB – 611: Ecology and Environmental Microbiology 1. 2. 9. 8. 5. 3. 4. Purification of enzyme from natural source by (any one method): Ammonium sulfate precipitation. Calibration of analytical instruments – Colorimeter and Spectrophotometer by estimation of biomolecules and statistical analysis of data generated. Organic solvent precipitation. Isolation of Aflatoxin producing organism 10. of cellulose degraders Isolation of chitinase degraders Isolation of pesticide degraders. 7. 2. COD and BOD Determination of TS and MLSS Simulated Waste decomposition using aerobic microorganisms. Determination of Km and Vm values of Invertase 5. Determination of DO. 6. Interpretation of Ramchandran Plot 26 . Electrophoretic Techniques: Protein electrophoresis by PAGE and SDS PAGE 7. pH. Isolation. Gel filtration 4. phosphate) on microbial species diversity. Determination of molar extinction coefficient of biological molecule 3.

Regulation of complement system – Classical and alternative pathway 4. Immunodeficiency disorders (pathophysiology. T-cell deficiencies. Role of IL–1 in immune activation and pyrogenesis d. antigen-antibody complexes. Negative regulation . Status of immune system in invertebrates and vertebrates with reference to diversity. Cytokine assays – immunological and functional assay systems 2.surveillance theory c. complexity and efficiency of cells and molecules. physiological. Super antigens and septic shock syndrome f. Immune adjuvant and tumor vaccine therapy. Immuno. Infective disorders: HIV-AIDS. Clinical Immunology (16) a. strategies for vaccine development) b. Types and general properties of cytokines and chemokines. diagnosis and prognosis) – i. Cytokines (6) a.αβ and γδ TCR.SEMESTER – III Theory course I . T cell mediated suppression of immune response b. T cell accessory membrane molecules (CD and adhesion molecules). anatomical organization. myeloma. b. Network theory and its experimental evidence c. Functional and structural evolution of immunoglobulin 5. biochemical and immunological properties. complement deficiencies 27 . Herpes infections ii. Non-infective disorders: Phagocytic deficiencies. Role in immune activation: TCR-CD3 complex and signal transduction pathways 3. Diagnosis of tumors – biochemical and immunological tumor markers d. Regulation of Immune Response: (6) a. Mechanisms of tolerance induction. Cellular transformations during neoplastic growth. protozoan and parasitic infections. Host immune response to tumor – Effector mechanisms. Approaches in cancer immunotherapy: Immunomodulation (definition and concept).Immunological tolerance. Biological Response Modifiers (BRMs) and their application in cancer therapy and in other diseases 6. Classification of tumors based on histological. Regulation of immune responses by: antigen. Immune System Evolution: (4) a. and combined deficiencies. humoral deficiencies. Escape mechanisms of tumor from host defense. Cytokines in therapy and disease. Immunoregulatory role of cytokines (in particular IL-4.MB – 701: Immunology 1. T-Cell Receptor: (4) Structure and types . Diversity of TCR (gene organization and rearrangements). Source and effect of Tumor necrosis factors and Interferons c. IFN-γ and TNF–β) e. Hodgkin’s disease) b. Tumors of lymphoid system (lymphoma. Immunity to infection – immune mechanisms to intracellular and extra-cellular infectious agents (with examples of bacterial. characteristics of cytokine receptors and antagonists b. Tumor Immunology: (8) a.

Animal models for autoimmunity and AIDS References: 1. Nature Immunology 10:981-986 14. 19. 3. David A. Systemic Lupus Erythomatosus (SLE). Hughes Eric A.. Butterworth and Heinemann Ltd.. (1983) Text Book of Immunology 4th edition. transgenic animals). 435|2:605611Bendelac Albert. Location.. NY. Barret James D. (1966) Fundamentals of Immunology. Steven M. W. 11. M. 16: 325–328 12. Short Text book on Chemical Pathology. Tetsuji Naka and Masato Kubo.c. strategies for immunotherapy (cytokine and vaccine therapy) and stem cell therapy 7. Nature Rev. (2007). Ltd.. Nature Reviews. Myasthenia gravis d.. 8. Ltd. Pareen Publications Bombay. 24: 497–518 20. and Jorge E... ELBS. Nature Reviews. Bangalore. Rev. The Evolution of Adaptive Immunity. (1992) Human Physiology Vol. Lange Medical Publications. (1993) Principles of Molecular and Cellular Immunology. and Luc Teyton. functional assays for phagocytosis In vivo systems – Experimental animals in immunology research (Inbred animal strains. Savage. and Palan V. 16. 4. hemolytic plaque assay. ELBS. 1 &2. Boyd William C. Interscience Publishers. V. Singapore 28 . Kuby J. 7:454-465 2. Freeman & Co. (1997) Immunology .Essential and Fundamental. Pathak S. Biotechnology by open learning series (BIOTOL). and Hall J. C. 13. H. Utz1. P. Fudenberg H. Michael C Carroll.. Cytokines and interventional immunology. C. (1982). 7. Therapeutic aspects in immunopathology – chemotherapy. and Wood K. C. J. SOCS proteins.. An array of possibilities for the study of autoimmunity. Garrison Fathman1 C. (1996) Immunology 3rd Ed. Medical Allied Agency. Mosby & Co. The Biology of NKT Cells Ann. (2005). Maruzen Asia Pvt. (1984) Essentials of Immunology. Ltd. Hafler. Hypersensitivity disorders (pathophysiology. Immunology. Immune Response to Salmonella: Location. (1996) Text Book of Medical Physiology. London. Luis Soares. ELISPOT assay. Stobo J. Experimental Immunology: (4) In vitro systems – kinetics of antigen antibody reactions. Brostoff J. (2004). S. Austyn J. Chan1 & Paul J. Zeev Pancer and Max D. Calcutta. 17. cytokine signaling and immune regulation. Defense Mechanisms. Guyton A. New Delhi. (1988) Essentials of Immunology. 7: 423 9. H. London.. Publishers Pvt. Stites D. Ann. Roitt I. The complement system in regulation of adaptive immunity. P. 15. 18. (1993) Immunology 6th Ed. Akihiko Yoshimura. Immunology. London.. Baron D. Vikas Publishing Pvt. Chatterji C. (2006). Cooper. G. Paul B. diagnosis and prognosis) – Asthma. 4th Ed. Immunity. Location?. Immunol. Immunol. Oxford 6. 5. M. New Delhi. (1983) Handbook of Immunology. Goel Book Agency. Roitt M. 25:297–336 10. V. and Wells J. N. (1993). Talwar G. Basic and Clinical Immunology. Male D. P. (2007). London. Oxford University Press. D. (2002). E. Mosby & Co. Galan. (2007). Rev. Roitt Evan.

Src kinase. Transforming viruses. Tumor suppressors. Proteins involved in recombination: RecA. DNA methylation and gene imprinting 5. V-onc and C-onc genes. Tumor suppressor genes b.SEMESTER – III Theory course II . Pseudogenes . DNA damage and repair: (06) a. coli DNA polymerases. B. Double strand break repair model c. Eukaryotic DNA replication. Transposable elements in bacteria. Gene clusters. Gene conversion 5. recombination repair. eukaryotic DNA polymerases. Controlling elements in Tn A. cot curves. Apoptosis.chromosome. C value paradox and genome size. Double strand break repair. replicative. . Nucleoid. regulation of replication. Oncogenes and Cancer: (08) a. multiple replicons. nucleosome. DNA Replication: (10) a. histone modifications . oncogenes and protooncogenes.MB – 702: Molecular Biology I 1. Cot ½ and Rot ½ values. types of E. Different types of DNA damages. Recombination: (06) a. Origin of replication. IS elements. Phosphorylation and its effect on structure and function of chromatin 4. connection of replication to cell cycle b. Nucleotide excision repair. chromatin organization and remodeling. Signal transduction. DNA replication in E. Homologous and site specific recombination b. telomere 3. details of replication process. Origin Recognition Complex (ORC). C. c. Models for homologous recombination: The Holliday model. Super-families 2. C d. b. Types of histones. Gene families. centromere. regulation of replication 3. 6. Different models for replication of linear and circular DNA. Eukaryotic genome. Base excision repair. repetitive and non-repetitive DNA sequence. DNA methylation and cancer.Methylation. Molecular markers of tumor 29 . Retroviruses and retrotransposon. ARS in yeast. Immortalization / transformation. Gene translocation. coli. Ty elements in yeasts d. Ruv A. Tn 5 and Tn 10 transposition c. B. Acetylation. Mobile DNA elements: (06) a. SINES and LINES 6. RB and p53 protein. Prokaryotic genome. Structure of chromatin. mismatch repair. D. SOS operon. Genome organization: (12) 1. composite transposons. C-myc. non-replicative transposons. Mu transposition b. Organelle genome 2. transcription coupled repair 4. Ras pathway. metastasis. higher order organization . replication features of single stranded phages.

Stephen P. Bruce Alberts. Peter Walter. 2nd Edition. Garland Publishing 3. Dennis Bray. Martin Raff. 2. Garland Publishing 4. Inc. Julian Lewis. 5. Molecular Biology of the Cell.References: 1. Pearson Education. Alexander Gann. (2007) Molecular Biology. Raff. 4th Edition.. Keith Roberts. Tania Baker. 4th Edition. Watson (2004). Benjamin Lewin. 5th Edition. Weaver R. 30 . and James D. Michael Levine. (2003) Essential Cell Biology. McGrew Hill Science. Jones and Bartelett Publishers Inc. and Dorling Kindersley Publishing. Inc. Keith Roberts. Bell. (2008) Genes IX. Richard Lodwick (2004) Molecular Biology of the Gene. Watson James D..

Nucleic acid based diagnosis: Nucleic acid hybridization. EID50. Infectivity assay for animal and bacterial viruses . Viral Vaccines: Conventional vaccines – killed and attenuated. Direct methods of detection – light microscopy (inclusion bodies). Modern vaccines – DNA vaccines. Structural proteins – envelope proteins. 2008) B. matrix proteins and lipoproteins d. suspension cell cultures and monolayer cell cultures d. Enveloped and non-enveloped viruses b. e. Radioactive Immuno precipitation Assay (RIPA). single stranded – positive sense and negative sense) e. peptide vaccines. Virus related structures – viroids and prions 2. Flow cytometry and Imunohistochemistry. hemagglutination and hemagglutination-inhibition tests.plaque method. RNA (double stranded. Plants and plant cell cultures 3.primary and secondary cell lines. end point methods. In ovo: using embryonated chicken eggs b. TCID50 g. Sampling techniques b. polyhedral and helical c.MB – 703: Virology A. Newcastle (Ranikhet) disease and Marek disease in poultry d. LD50. Infectivity assays of plant viruses 4. In vivo: using experimental animals c. Herpes Viruses. neutralization. anti-idiotype vaccines. Immnuodiagnosis. Processing of samples – Enrichment and concentration c. b.SEMESTER – III Theory course III . Viral genomic organization and replication – types of nucleic acid DNA (double stranded and single-stranded). Simian Virus 40 c. Cultivation of viruses: Growth of viruses in – a. polymerase chain reaction. Protein nucleic acid interactions and genome packaging f. Capsid symmetries – icosahedral. electron microscopy and fluorescence microscopy d. Ex vivo / In vitro : using various cell cultures . immunomodulators (cytokines). Rinderpest disease in cattle 2. Complement fixation. subunits vaccines. Structure of viruses a. pathophysiology and epidemiology for the diseases caused by: a. microarray and nucleotide sequencing. ICTV nomenclature and classification of viruses (as per 9th Edition. General Virology: (16) 1. Viral Diseases in Animals: (12) 1. a. LINE probe assay f. General characters and genomic structure. Western blot. pock counting. adjuvants to increase immunogenecity of vaccines 31 . recombinant DNA/protein vaccines . ID50. Diagnostic and detection methods a. edible vaccines.

virus free planting material. New York.. (2002). R. Mechanism of cell transformation by RNA viruses and by DNA tumor viruses. cellular sites of virus replication and assembly. Haaheim L. et. vector control. (2003).contact. Martin. Racaniello. 2nd Ed. R. Antivirals: Interferons. John Wiley and Sons. (2004). seed and pollens 5. Lamb. (1998) Evolution of RNA Viruses Ann. Howley. Effects of viruses on plants: Appearance of infected plants. 5. 42: 657 – 83 32 . indicator plants c. disease forecasting 6. Lambda and M13 phages 2. Bernard Roizman. Cauliflower mosaic virus D. Chapters 3-13 3. Blackwell Publishing 2. V. 7. Luria S. J. Edited Retrovirus mediated oncogenesis C. Rev. Oncogenic viruses: Virus induced cell transformation and oncogenesis. Transmission of plant viruses: a. (2007). E. R. Antigen based methods d. Hewlett. Pathogenesis. designing and screening for antivirals. Phage therapy for control of bacterial poultry diseases References: A. Without vectors . Straus J. Skalka.b. American Society Microbiology. Bacteriophages: (8) 1. General Virology 1. M.. Principles of Virology: Molecular Biology. and Control of Animal Viruses. Lippincott Williams & Wilkins 6. Viral Disease in Plants: (12) 1. Malcolm A. John Wiley & Sons. (1978) General virology. Methods for detection of plant viruses: a. Morphology. W. In seeds. seed stocks and diseased plants b. Through vectors . Field's Virology. 5th Ed. International Congress on Taxonomy of Viruses: http://www. Enquist.. Wagner. Stephen E. release and translocation of virus particles in tissues 3. A. Flint S. nematodes and fungi b. Griffin. J.nlm. Pattison and R. biochemistry of virus replication. mechanisms of action. Martinez J. Basic Virology. Life cycles of plant viruses – TMV. H. and Straus E. Histopathological methods 4. Knipe David M. V. Peter M. Straus. 4. Prevention of crop losses due to virus infection . Microbiol. physiological and cytological changes in infected plants 2. Whitley. antiretrovirals — mechanism of action and drug resistance 3. 3rd Ed. genome organization and life cycles of – T (odd and even). A Practical Guide to Clinical Virology. Rancaniello.insects.ncbi. J. histological. Behavior of viruses in plants: Early stages of infection. Robert A. Diane E. R. L. Edward K.

W. Racaniello. B. Oxford University Press 3. V. Vaccines.J. (1998). Biochem.. Elsevier Science & Technology Books. DNA virus Replication.. Rev. W. & Marion P. (2004). Scientific American (1):48-57 4. (2002). and Immunotherapies. (1998) Assembly of animal viruses at the cellular membrane Ann. Alan Warnes. Modern Microbial Genetics. Veterinary Virology.54:531-64 5. Matthews R. Modern Microbial Genetics. Prusiner S.. Influenza. Rev. Enquist. (1999). Potter C. Walter A. Stephenson John R. Plotkin Stanley A. Hillar O. M. Rancaniello. (1985) Viroids Ann. Streips and Ronald E.45:475-508 6. Sherkar A. 127-143 3.. Marcel Dekker C. (2002). Academic Press 6. Skalka. 39:451-74 5. (1995) The Prion Diseases. Paul J. Virology Methods Manual. 2. 85-126 33 . Diagnostic Virology Protocols: Methods in Molecular Medicine. Hendrix R. Douglas John. Tyring. Bacteriophage λ and its relative. Reisner D. Yasbin. Guttman Burton S. Rev. and Elizabeth M. Pathogenesis. J. and Compons R. Elsevier Science 8. Brian W.W. Flint S. Kutter.E. Microbiol. Uldis N. R.J. & Gross H. Orenstein. Kangro. Bacteriophages. 2nd Ed. Uldis N. Cann Alan J. A. Vaccines. Stephens B. Editors. Stephen K... Marian C.L.B. (Editor). Gibbs Adrian & Bryan Harrison ( ) Plant Virology -The Principles. Studdert. Streips and Ronald E. Microbiol. Davis and Dulbacco Medical Microbiology 2. Gibbs. Animal Viruses 1. Yasbin. Mahy. and Control of Animal Viruses. Michael J. (2003). W. (1996). E. Chapman and Hall. American Society Microbiology 4.. 2nd Ed. Rev. R. L. Plant viruses 1. Ann. (1975). Elsevier Health Sciences 7. Murphy Frederick A. Humana Press 9. Editors. Bacteriophage Genetics. (2002). Horzinek. (1991) Hepo DNA viruses and Hepatocellular Carcinomas. Principles of Virology: Molecular Biology. Wiley-Liss Inc. WileyLiss Inc. Edward Arnold Press 3. Microbiol. Bacterial viruses 1. (1985) Viral Taxonomy for the Non virologists Ann. V. Antiviral Agents.42:489-519 D. London 2. (2000). H.F. (2003).

MB – 711: Microbial Technology 1. Israilide. mycoinsecticides (Trichoderma) and cell count determination on time scale 3. Parameter testing for immobilized enzyme columns: a. Whittaker A. 34 .. Effect of cell concentration on enzyme activity 5. Biosorption of dyes or metals using dead biomass. Principles of Fermentation Technology 2nd Edition. Hall S. (1995) Practical Biochemistry. Differentiation of callus culture in to shoot / root. 7. (1998). yeast cells immobilized in calcium alginate beads 4. Estimation of antimicrobial activity using standard guidelines (NCCLS/CLSA) 8. Elsevier Science Ltd.SEMESTER – III Practical course I . Christian Barnett. Stanbury P.J. 9. infectivity assays in indicator plants References: 1. Pullulan production by Aureobasidium pullulans growing on hydrolysed potato starch waste. Dried biomass to be used for biosorption (both the organisms are suitable for adsorbing Congo Red). Wilson & Walker. and activity fractionation.. Studies on laboratory scale production of exopolysaccharide (Pullulan gum) and microbial emulsifiers – using suitable production strains (obtained from culture collections). 3. Effect of gel concentration on enzyme activity c. Extraction and estimation of bioactive (antimicrobial) principles from plants. Comparative enzyme activity of free cells and immobilized cells b.. 2. Media optimization for large scale production (effect of medium composition on any one of the products 6. Preparation of bioinoculants – phosphate solubilizers. Bernard Scanlon and Cleanthes J.g. harvested and killed by autoclaving. Principles & Techniques. Bioassay and Chemical estimation of penicillin 2.F. Preparation of enzyme immobilized columns for biotransformation –e. Aspergillus niger or brewer’s yeast cells could be grown in liquid media. Alan Smith. Study of plant virus diseases: Collecting data and samples (preparation of herbaria). Preparation and maintenance of plant callus culture. 10.

intra venous. yolk sac. allantoic fluid. Determination of molecular weight of plasmid DNA. (1994). Restriction digestion and ligation of DNA. P. Endonuclease mapping of DNA 4. and Brent R. (1988). chorioallontoic membrane References: 1. M. Cold spring harbour NY: cold spring harbour laboratory press. John Wiley & sons Inc. Vikas Publishing Pvt. Ltd. PCR amplification of desired gene 3. Agglutination techniques: Preparation of O and H antigen of Salmonella and its testing using known antisera. coli. Precipitation reactions of antigen-antibody: Immunoelectrophoresis –rocket immunoelectrophoresis. Ausbel F.MB – 712: Molecular Biology and Immunology Molecular Biology 1. Egg inoculation technique by various routes .embryo. NY 5. Plummer David T. Plasmid DNA extraction and agarose gel electrophoresis. Current protocols in Molecular biology. Ltd.. 5. Ausbel F. Curing of plasmid using agents such as Ethidium bromide. An introduction to practical biochemistry. M. Lymphocyte culture and detecting proliferation on mitogenic stimulus 4. Talwar G. Tata McGraw-Hill Publishing Co. Separation of lymphocytes. amniotic cavity.. NY 35 . Maniatis T (1989) Molecular cloning – a laboratory Manual 2nd ed. 3rd Ed. Single and Double diffusion techniques 2. Short protocols in Molecular biology. New Delhi 3. New Delhi. ( ). intra cerebral etc. John Wiley & sons Inc. Preparation of primary cell line from chick embryo 5. intra muscular. intra dermal. Plumbagin and Mitomycin C. Fritsch E F. Preparation of competent cells and transformation of plasmid DNA in E. 109-121 2.. Immunology 1. Acridine orange. 2. and Brent R. Titre determination of isoantibodies to human blood group antigens. 6. (1983) Handbook of Immunology. 4. Animal inoculation technique by different routes: Subcutaneous.. demonstration of prozone and postzone phenomenon 3.SEMESTER – III Practical course II . Sambrook J.

g. Purification and Characterization of bioactive molecules from natural resources. Development of antimicrobial agents: (12) a. Clinical studies: Phase I. b. sulpha drugs. Candidate drug selection d. Current approaches to drug discovery: Rational Drug design. synergism and additive effect) c.nephro. Methodologies for testing of antimycobacterial. arsenicals b. Combinatorial chemistry. Phases of drug discovery: Bioprospecting. Clinical development of biologicals: (8) a. Screening and development strategies for new antimicrobial agents with respect their mode of action and studying the mechanism of resistance.) b. Historical perspective – Paul Ehrlich’s postulates. Principles of Extraction. Outcome of trials c. antiparasitic and antiviral drugs (in vivo and in vitro infectivity models). Preclinical development: i. Drug metabolism – activation / inhibition of drug in vivo. etc. receptor / target concept in drug designing. Introduction to pharmacogenomics. Sustained release drugs d. Drug distribution in body.g. Mutagenecity (Ames test. bio-availability and pharmacokinetic studies 3. Factors affecting bioassay. GCP. Delivery systems – formulations. phase III and phase IV of clinical trials – Objectives. Safety profile of drugs (Pyrogenecity. Bioassay of antibacterial agents in liquid media and in agar media using standard guidelines (e. Laboratory methods to assess activity of antimicrobial combinations (antagonism. 36 . Conduct of trials. Validation (GMP. Regulatory authorities for introduction of medicines in market – Role of Food and Drug Administration. nucleic acid and protein metabolism. phase II. Acute. Lead compound optimization. Carcinogenicity and Teratogenecity e. Case studies of development of drugs e. -cardio and -neurotoxicity) ii. . Drug discovery (12) a. Lead discovery. targeted drug delivery. adverse drug reactions 2.MB – 801: Pharmaceutical and Medical Microbiology A] Pharmaceutical Microbiology (The focus of the following topics should preferentially be the drugs against microorganisms and from micro-organisms) 1. FDA guidelines for drugs / biologicals. Toxicity –hepato. based on examples of established drugs acting on bacterial cell wall. Drug interactions. micronucleus test).SEMESTER – IV Theory course I . Toxicological evaluation of drug: LD50. National Committee for Clinical Laboratory Standards (NCCLS) / Clinical and Laboratory Standards Institute (CLSI)). GLP. antifungal. High Throughput Screening c. subacute and chronic toxicity iii. cell membrane.

John Wiley and Sons. and Kalman S. Cundliffe E. John Wiley and Sons Inc. Association. Toxins (diphtheria. 8th Ed. Goldstein A. Analytical Microbiology Volume I and II.. Introduction to pharmacology. Himalaya Publishing House. Colonization. Wiley-Interscience. cholera. Combinatorial Biocatalysis: Taking the Lead From Nature. The molecular basis of antibiotic action. Drug discovery and development Volume I: Drug discovery. Chatwal G. H.. Opin. tetanus toxins and endotoxins of Gram negative bacteria – mode of action and in vivo and in vitro assay systems). (1963). (1969) Principles of Drug Action. Kokate C. Agarwal S.. Blackwell Scientific Publication.. (1972). Siderophores. London 9. H. CRC Press 37 . A step wise process of infection – Crossing physical. (2006). F. A. Mechanisms of bacterial resistance to host cellular (phagocytosis) and humoral defenses 3. virulence genes and pathogenecity islands References: Pharmaceutical Microbiology 1. A. P.. 4th Ed. Foreman and Tai-Pang D. K. capsule. CRC Press 13. and Paridhavi M. Graly John O. Blackwell Scientific Pub.. 12. Text book of Immunopathology. Lorian. Invasins (Fibrinolysins. Handbook of Phase I / II clinical drug trials. and D S. USA. Altreuter D. B. Kinins). (1997). Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA). Richmond M. London 11. Chorghade Mukund S. Dewick Paul M.. Universities Press (India) Pvt. hypal extensions). 2nd Ed. Molecular basis of bacterial pathogenecity – cytoskeletal modulation of host cell. (1999). Rawlins. and Pieter H. 130. Evasins (catalase.B] Medical Microbiology (16) Mechanisms of Bacterial Virulence: 1. (2000) Pharmacology. Dale Maureen M. 3rd Ed. (1974) Pharmacology of Powdered Crude Drugs. Reynolds P. Joubert. Ltd 2. (1986). (2007). coagulase. 3. 7. Burn J. London 4. Williams & Wilkins Publication 17. and Waring M. Editior E.. (2008). Mumbai. (1004). www. London 15. Oxford. Academic 8. John C. Purohit A.. The Basis of Pharmacology. Adhesion and Invasion of host tissue and toxigenesis. Bentley’s Textbook of Pharmaceutics. J. BAilliere Tindall. O. Herbal Drug Technology. Nirali Prakashan.with details account of virulence factors – Adhesins (pili. 5. Harper international edition New York. Ltd. Antibiotics in laboratory medicine. Leucocidins. Gokhale A. Iyengar M. 16. Fan. Kavanagh Frederick. Clark. (2003) Biopharmasceutics and Pharmacokinetics. 10.. Holliger. 2... chemical and biological barriers. Mannfred A..V. S. John Wiley and Sons 10. Gale E. Aronow L. E. 6. Curr. (2002). M. hemolysins... P. Manipal 14. 3rd Ed. hyaluronidase.cpcsea. Medicinal natural products: A biosynthetic approach. (1957) Principles of Therapeutics. (2002). 2nd Ed... Biotechnol. hemagglutinins).

S.. 21. Micheles P. Nature Rev. Bacterial pathogenomics. National Committee for Clinical Laboratory Standards (now Clinical and Laboratory Standards Institute. Dordick and D. 24. (1994). 79230 7. And Richard A. Galan. 12th information supplement (M100-S1). and David W. CLSI). and Jorge E. (2007). Y. Schlessinger David.. 449|18: 835-842 8. E. (2002). (2003). 10(11):483-5 3. Satoskar R. (1996). Eduardo A. New Delhi Medical Microbiology 1. Editor.18. Identification and analysis of bacterial virulence genes in vivo.Immunology and Serology. (2002). Synthetic Combinatorial Libraries: Novel Discovery Strategy forIdentification of Antimicrobial Agents. Walsh Gary.. Trends Microbiol. Nature Rev 449/18:827-834 10. Phil. A Natural Approach to Drug Discovery. American Society for Microbiology. Pharmaceutical Biotechnology. Clark. C. Villanova. 12th Ed. S. CBS Publishers and Distributors. John Wiley & Sons Ltd. 2nd Ed. Bhandarkar (1991) Pharmacology and Pharmacotherapeutics. Holden. CLSI). Osol Arther (1975) Remington’s Pharmaceutical Sciences. 16. Mack Pub. 355. C. S. (1998). 20. Carpenter Philip L. in Microbiology – 1979. 2(8):289-294 4. London B. Methods for dilution antimicrobial susceptibility testing for bacteria that grows aerobically. Fredricks and David A. Wren. Trends in Microbiology. D. Soc.roisman and Howard Ochman. Washington D. American Society for Microbiology. Vyas S. Saunders International Edition . (2007). Bhavsar Amit P. (2000). 197. W. NCCLS: 2002 22. Approved Standards M7-A4.. Ames. (2002). Location?. Sequence-Based Identification of Microbial Pathogens: a Reconsideration of Koch’s Postulates. 1997. 15th Ed. Maron Dorothy M. Schlessinger David. Editor. Villanova. Biochemical Genetics of Pathogenecity. Immunity. David N.. Mark J. P and Dixit V. Unsworth K.. 1 & 2. Houghten. R. Performance standards for antimicrobial susceptibility testing. Mutation Research. Vol. PA: NCCLS. Popular Prakashan. Mumbai. Woods D. Pennsylvania. (1975). Location.. National Committee for Clinical Laboratory Standards (now Clinical and Laboratory Standards Institute. Khmelnitsley. Revised methods for the Salmonella mutagenecity test. 613-622 2. 113:173-215 19. How to become a pathogen. London 5. Mechanism of Microbial Virulence. 79-230 6. Trans. Combinatorial Biocatalysis. Relman. England 26. Manipulation of host-cell pathways by bacterial pathogens. B. R. Guttman and B. (1983). Biopharmaceuticals Biochemistry And Biotechnology. 16: 325–328 9.. L. in Microbiology – 1979. PA. Immune Response to Salmonella: Location. Sylvie E. The use of animal infection models to study the pathogenesis of melioidosis and glanders. and Bruce N. Antimicrobial Agents and Chemotherapy. Hughes Eric A. Brett Finlay. Pallen1 & Brendan W. Saunders and Co.. G. (1996). J. 1067–1071 25. 18–33 38 . Enrique Pe´Rez-Paya. Blondelle. Clinical Microbiology Reviews. Co. Washington D.. S.. E. & S. Julian A. 23. Trends in Biotechnol.

RNA editing. gene library. Eukaryotic RNA polymerases I.inteins. (04) 39 . Real time PCR and its applications. Gel retardation assay b.SEMESTER – IV Theory course II . Expression vectors. Automated sequencers. rRNA synthesis and its regulation d. plants. amino acyl t-RNA synthetase c. b. Molecular chaperons iii. Enhancers. Ribosomal structural components. Enzymes used in recombinant DNA technology. elongation and termination events (Rho dependent. Structure of bacterial RNA polymerase. PCR. DNA microarrays and their use in genomics b. codon usage b.lambda vectors. Translation: i. single strand vectors. group II introns. Transcription (12) a. RNA interference. Co-translational / post-translational modifications. Role of sigma factor.initiation. characteristics of genetic code. Southern blot. Transcription . yeast. Processing of RNA: RNA splicing. Pyrosequencing and recently developed sequencing methods. termination of translation ii. II and III and their promoters. exteins iv. phages.. Protein sequencing 5. Concept and meaning of genome projects and their applications. Rho independent termination). high capacity vectors: PAC. Genetic code and Translation (12) a. Gene annotation. Regulatory RNA: antisense RNAs. t-RNA: Structure. selection and screening of recombinants.initiation. Heterologous gene expression c. Sanger’s di-deoxy method and modifications. Typical bacterial promoter. comparison of eukaryotic and prokaryotic ribosomes.use of radioactive and non-radioactive nucleotides for probe preparation and detection of hybrids. Sequencing techniques: Maxam and Gilbert method. cosmid. cDNA Cloning b. micro RNAs. attenuation 2. modified bases in t-RNA. RT-PCR. and animals). BAC and YAC 4. noncoding RNAs and their role e. Protein splicing . Northern and Western hybridization technique . Cytosolic protein degradation 3. Capping of mRNA and polyadenylation d. Eukaryotic transcription regulation based on mRNA stability and localization. code alignment. Recombinant DNA Technology (10) a. Translation process in prokaryotes and eukaryotes. Phage display systems. c. deciphering the genetic code. I. shot gun cloning. Techniques used in RDT: (10) a. DNA foot printing.MB – 802: Molecular Biology II 1. t-RNA processing. TATA box Binding Protein (TBP) c. Genome project: a. Preparation of recombinant DNA and its transfer to appropriate host (bacteria. active centers of ribosome. Vectors: Plasmids. Historical approach. elongation. altered code in mitochondria.

5. Garland Science 6. Watson. 3. 2. 4. Wink Michael. Blackwell Science Ltd.. (2007) Genome 3. Jones and Bartelett Publishers Inc. Richard Twyman. McGrew Hill Science. Michael Levine. Strachan T. Bob Old (2001). Bell. James D. An introduction to molecular biotechnology. Pearson Education. Weaver R. 5th Edition. Inc. Richard Loswick (2004) Molecular Biology of the Gene. Sandy Primrose. 6th Edition.References: 1. Garland Science. Ed. 4th Edition. and Read A.. Alexander Gann. 3rd Edition. Principles of Gene Manipulation.. Tania Baker. and Dorling Kindersley Publishing. (2008) Genes IX. 3rd Edition.. Stephen P. Human Molecular Genetics. Brown T. Benjamin Lewin. Inc. (2004). 7. (2007) Molecular Biology. Wiley-VCH Publications 40 .

Immobilized cell reactors and air-lift reactors – Design and operation. Flow patterns with different types of impellers. Effect of type of growth on fermentation: The type of growth (mycelial pellet form. temperature. II] Processes (20) Upstream. efficiency etc. Power number. baffles and impellers.MB – 803: Microbial Technology I] Principles of Bioengineering: (20) 1. Kinetics of growth and product formation (growth rate. and the operational aspects such as working volume. 2. yield coefficient. advantages and limitations of each type. pH. DO and DCO2). Microbial enzymes (Chitinase. Glucose Oxidase.) b) Operational modes of bioreactors: Batch. membranes and cytoskeleton) 41 . Fed-batch and Continuous processes: Applications. and their combinations) b.Functions of agitation. c. nutrient and oxygen transfer. a) Growth and product formation during fermentation: Concept of primary (growth associated) and secondary (growth non-associated) metabolites and their control. biosensors and fuel cells (Architecture of the fungal cell: cell wall. Monitoring of process variables: Use of various types of sensors and biosensors for monitoring environmental parameters (pressure. Antibiotics (Rifamycin) 2. Use of immobilized cells / enzymes to produce protease 5.Design aspects CSTRs: The dimensional ratios of the outer shell.SEMESTER – IV Theory course III .Theory of oxygen transfer in bubble aeration. Lipase) 3. oxygen and heat. The configuration (placement) of impellers in a vessel and the different types of impellers (types of turbines and propellers. b. Aeration number< working out examples using different softwares 3. Oxygen Transfer Rate OTR. Use of fungi in industry including food industry. types of biosensors 4. Exopolysaccharides (Pullulan) 4. mycelial filamentous form. Fermentation and Downstream Processing for the following: 1. 5. Agitation . Fermentation broth rheology and power requirements for agitation – Concept of Newtonian and non-Newtonian fluids. cells producing exopolysaccharides) affects mass transfer of nutrients. as also cell proliferation can be affected by shearing of cells. Aeration and agitation: a. At least one example of each type may be explained to show these effects in any suitable fermentation. Oxygen transfer kinetics (Oxygen Uptake Rate –OUR. effect of broth rheology on heat. free cell. Ccrit). Reynold’s number. determination of KLa. Bioreactor design and operation: a. Designing of bioreactors . Aeration . Basic principles of operation.

Prescott and Dunn’s Industrial Microbiology . John Wiley and Sons. Wetter L. P. Canada 17.(1985) Topics in Enzyme and Fermentation . CBS Pub. Moo-Young M. 10. Komamine A. (1992) BIOTOL series. Bioremediation and Biological control. (1986) Secondary metabolism in plant tissue cultures. Council. operation and validation for analytical methods IV] Intellectual Property Rights (IPR): Basic concepts of IPR Introduction to forms of IPR – Patents and Designs References: 1. D’ Elia and K. A. Ponkhshe S. Res. Van Damme E. (1992) Pharmaceutical Biotechnology: Fundamentals and Essentials. Ltd. 14. Bioreactor Design and Product Yield (1992). (1985) Comprehensive Biotechnology Vol: III & IV. Nelson (Eds.J. Pune 2. Cambridge Univ. Press. Peppler H. A and Fowlew M. (2001) Basic Biotechnology 2nd Ed. Biochemical Engineering. 7. and D. Butterworths Heinemann. M. Ed. Shuichi and Aiba. Cambridge Univ.Pergamon Press. Academic Press New York. Equipment and Facilities. Academic Press.. 7.H.E and Groves M. Patent. (1982) Plant Tissue Culture methods. (1991).W eds. M. New York. Natl. Doran Pauline (1995) Bioprocess Engineering Principles. Operational Modes of Bioreactors. Bhate and Ponkhshe Prakasham. a. Klegerman. BIOTOL series. Interpharm Press Ltd. Marris. Exercises on preparation of SOPs. 4. J. J. N. Cambridge 8. Vol. Scragg. Press. (1984) Biotechnology of Industrial Antibiotics. ed. Misawa M and Dicosmo F Eds. 18. Animal cell culture technology to produce recombinant vaccines III] Principles of Validation Process / Method Validation: (4) a) The concept of ISO Certification. 11. 1982. Tokyo 6. Cambridge 13. Standford. Perlman (1970) Microbial Technology Volume 1 and 2. 15. Bhate and Pongashe. Lydersen B.. Wiseman A. b) Preparation of SOPs c) Validation protocols for methods in Quality Control d) Process validation The above should be discussed within WHO Norms. Plant cell culture in Japan. New York. Academic Press.6. N. (1988) Management of Intellectual Property. CMC Co. Bhate Prakashan . John Wiley and Sons Inc. Ratledge C and Kristiansen B eds. Marcel Dekker Inc.Biotechnology. Reed G.) (1993) Bioprocess Engineering: Syatems. Buffalo Grove IL (4) 5.. 3. 16..Y 9. 42 . New Delhi.. Pune 12. Butterworths Heinemann. 4th Ed.R and Canstabel eds. 1 and 2. Use of fungi in agriculture and environmental applications: Biofertilizers.

Writing a pedagogical (academic) article on a scientific theme 6. Writing a newspaper report / popular article of a latest research paper. 5. Photomicrography. Representation of and calculations for data obtained 5. Writing monthly progress reports / synopsis / interim reports 7.MB – 811: Research Methodology I (Dissertation) Scientific communication: Scientific writing (The objective of this practical will be preparing a research paper based on results of the dissertation work. Experiment planning 3. Experimentation.SEMESTER – IV Practical course I . writing footnotes to figures and tables 8. All the following aspects can be included in the final report and presentation of the dissertation work): 1. Writing a Masters thesis 8. Literature review (and choosing a suitable topic) 2. Interpretation of data with the use of statistical tools (if required) 6. 2. The data generated through the dissertation work of student should be used for this exercise. Title and abstract for a given text. Drawing appropriate figures on given data. Making Power Point slide shows SEMESTER – IV Practical course II . Preparation of display material (such as scientific posters) 9. Scanning pictures.MB – 812: Research Methodology (Dissertation) Dissertation carried out by the students should exercise the following steps in a systematic manner. (Any refereed journal may be used for sample ‘Instructions to Authors’) 4. taking photographs of experimental results 10. Critically comment on a manuscript 7. Making OHP transparencies. Writing the paper based on a given set of instructions to authors. under the supervision of practical-in-charge / project coordinator 1. Choosing and indexing key words from a given paper 3. Presenting the thesis in an ‘Open Defense’ 43 . with the use of contemporary methods and standard protocols 4.

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