BIO 101 Lab Study Guide for Practical #1 1) Know the base units in the metric system for

r measuring length, mass and volume

2)

Know the units with the prefixes Mega-, kilo-, centi-, milli-, micro-, nano-

3) etc.

Be able to convert between the different metric units: kilo- to base unit, mega- to milli-,

4) temps.

Understand the Celsius scale for measuring temperature; know boiling, freezing and body

5)

Know the five steps to the Scientific Method

6)

Be able to define hypothesis, data, control, conclusion, theory

Lab#2: 1) Be able to name the four classes of organic macromolecules that make up living cells.

2)

Know the difference between dehydration synthesis and hydrolysis reactions

3)

Be able to define mono-, di- and polysaccharide, polymer, monomer, peptide bond,

enzyme

4)

Know the monomers of proteins, polysaccharides and triglycerides (fat molecules)

5)

Know what Benedicts reagent tests for, how to run the reaction and what a positive and

negative test look like

6)

Know what Biuret reagent tests for, how to run the reaction and what a positive and

negative test look like

7)

Know what an iodine test is for, how to run the reaction and what a positive and negative

test look like

8)

Know what a brown paper tests for, how to run the reaction and what a positive and

negative test look like

9)

Know what control you used and why you used it

10)

Be able to identify the plasma membrane, nucleus and fat globule on a microscope slide

11)

Know the function of fat, starch and glycogen

Lab #3: 1) Know all the cellular organelles and their functions from page 43

2)

Be able to identify the organelles on pictures and the plastic cell models in the classroom

3)

Know all the parts, and their functions, of the microscope on page 18

4)

Know what a stereomicroscope is

5)

Know what three organelles are present in a plant cell but not in an animal cell

6)

Know the two organelles that are present in an animal cell but not in a plant cell

7)

Know the difference between a prokaryotic cell and a eukaryotic cell

8)

Be able to identify a chloroplast and cell vacuole on an Elodea wet mount

9)

Know the steps to making a wet mount

10)

Be able to explain why the cover slip is placed slowly and from an angle on a wet mount

11)

Be able to define cytoplasm, nucleoplasm, cytoplasmic streaming

Lab #4: 1) Be able to define diffusion, osmosis, concentration gradient, selectively permeable

membrane,isotonic, hypotonic, hypertonic, pH scale, buffer, acidic and basic, solution, solvent, solute

2)

Be able to predict which way water will move through a selectively permeable membrane

based upon the concentrations of solute and solvent present

3)

Know how to read a piece of pH paper given the color scale

4)

Know that the osmotic concentration of living cells is equal to about 1% NaCl and be

able to determine if other solutions are hypotonic or hypertonic to living cells

5)

Know that water always moves from high concentration to low hypotonic to hypertonic

6)

Understand why a solute diffuses slower through a semisolid than through a liquid

7)

Know what plasmolysis is and why it happens

Lab #5: 1) Be able to define metabolism, reactant, product, substrate, enzyme, degradation,

synthesis, active site, denature, independent variable, dependent variable

2)

Know what the reactant and products of a catalase reaction are

3)

Understand the effect of temperature, concentration and pH are on a catalase reaction

4)

Be able to construct and read a graph representing catalase activity under different

conditions

5)

Know what the bubbles are and why they indicate catalase activity

6)

Know what our source of catalase was

7)

Be able to answer the Conclusion questions on page 83

8)

Be able to write the balanced reaction catalyzed by catalase

Lab #6: 1) Be able to write the balanced equation for photosynthesis

2)

Be able to define thylakoid membrane, grana, stroma, pigment, Calvin cycle,

chromatography, carotene, xanthophyll, chlorophyll

3)

Explain how you know that plant pigments are nonpolar molecules

4)

Explain what colors of light each of the pigments would absorb and which would be

reflected

5)

Explain the relationship between photosynthesis and respiration

6) applied

Explain why the level of solution in the photosynthesis tube changed when light was

7)

Using the graph on page 98, explain what wavelengths of light are used best by different

pigments

8)

Explain the results seen when you used green light for photosynthesis

Lab #7: 1) Be able to write the balanced chemical reaction for ethanol fermentation

2)

Be able to look at prepared respirometer tubes for fermentation and interpret the results

3)

Know which sugars tested were the best for yeast fermentation

4)

Understand the difference between fermentation and respiration

5)

Be able to write the balanced chemical reaction for cellular respiration

6)

Understand the reason KOH was added to the respirometers

7)

Be able to read the results on a side arm respirometer tube and know what is being

measured

8)

Know what was the control in the experiments

9)

Understand the difference between germinating and non-germinating seeds