SLandard ConcenLraLlon

chromlum vl (ppm)
volume chromlum vl
(ml)
Absorbance (nm)
SLandard 1 9Ŧ36 3 0Ŧ092
SLandard 2 31Ŧ2 10 0Ŧ369
SLandard 3 49Ŧ92 16 0Ŧ607
SLandard 4 78Ŧ00 23 1Ŧ094
SLandard 3 93Ŧ6 30 1Ŧ447
Sample waLer 0Ŧ321
WavelengLh ƹ 433 nm

M
1
v
1
ƹ M
2
v
2
ŦŦŦŦŦŦŦŦŦ (1)
M
1
ƹ 136 ppm ţ v
2
ƹ 30 ml

(136ppm)(v
1
)ƹ10ppm (30ml)
v
1
ƹ3Ŧ2 ml

lor easler plpeLLeţ round off Lhe volume Lo a whole numberţ whlch ls 3ţ Lhen calculaLe Lhe
concenLraLlon of chromlum vl agalnŦ

(136ppm)(3ml)ƹ(M
2
)(30ml)
M
2
ƹ 9Ŧ36 ppm

1he same goes Lo Lhe 2
nd
sLandardŦ
(136ppm)(v
1
) ƹ (30)(30 ml)
v
1
ƹ 9Ŧ61 mlţ round off Lo 10 mlŦ

(136ppm)(10ml) ƹ (M
2
)(30ml)
M
2
ƹ 31Ŧ2 ppm


3
rd
sLandard
(136 ppm)(v
1
) ƹ (30)(30ml)
v
1
ƹ16Ŧ02 ml

(136ppm)(16)ƹ(M
2
)(30ml)
M
2
ƹ49Ŧ92 ppm

4
Lh
sLandard
(136ppm)(v
1
)ƹ(80)(30ml)
v
1
ƹ23Ŧ64ml

(136ppm)(23)ƹ(M
2
)(30ml)
M
2
ƹ78 ppm

3
Lh
sLandard
(136ppm)(v
1
)ƹ(100)(30)
v
1
ƹ32Ŧ03ml

(136ppm)(30ml)ƹ(M
2
)(30)
M
2
ƹ 93Ŧ6ppm


1o flnd Lhe concenLraLlon of Chromlum (vl) ln Lhe waLer sampleţ flrsL we have Lo expolaLe Lhe graph
Lo flnd Lhe equaLlon of Lhe llnear llneŦ

lrom Lhe graph 1Ŧ1 ln Lhe resulLţ Lhe llnear llne equaLlon ls glven asť

y ƹ 0Ŧ0143x

1henţ by subsLlLuLlng Lhe absorbance value of waLer sample ln Lhe charLţ
x ƹ

x ƹ

x ƹ 22Ŧ43 ppm

1he value of x refers Lo Lhe concenLraLlon of Chromlum (vl) ln Lhe waLer sampleţ whlch ls 22Ŧ43 parLs
per mllllonŦ 1he waLer sample ls noL hyglenlc and noL sulLable for drlnklng or publlc consumpLlon and
noL even sulLable for agrlculLure purposes slnce Lhe waLer ls sald Lo be LoxlcaLed lf lL has
concenLraLlon of chromlum (vl) hlgher Lhan 0Ŧ100 parLs per mllllonŦ
RESULT



Craph 1Ŧ1











y ƹ 0Ŧ0143x
8² ƹ 0Ŧ9738
0
0Ŧ2
0Ŧ4
0Ŧ6
0Ŧ8
1
1Ŧ2
1Ŧ4
1Ŧ6
0 10 20 30 40 30 60 70 80 90 100
A
b
s
o
r
b
e
n
c
e

(
n
m
)
Concentrat|on of Chrom|um V (ppm)
Absorbence (nm) vs Concentrat|on of Chrom|um
V (ppm)
ECTIVE

O To determine the concentration oI Chromium (VI) contains in the standards and water sample
using a spectrophotometer.
O To standardize the solutions in the range oI 1 to 100 parts per million to be used in the
calibration oI the spectrophotometer using a proper method oI dilution. (M
1
V
1
÷ M
2
V
2
)
O nalyse the sample water whether it is suitable to be used Ior daily purposes.


CCLUSI

From the experiment, we can conclude that the concentration oI Chromium (VI) in the water sample
determined using a spectrophotometer is 22.45 parts per million. This water sample is said to be
contaminated since the value oI concentration oI Chromium (VI) is much higher than the standard oI
0.100 parts per per million (which is considered saIe). ThereIore, the water sample is not saIe and not
suitable to be used as drinking water and agricultural purposes.

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