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Wenzheng Yu AP Biology Period 1 Lab Eleven: Animal Behavior Introduction The overall purpose of this lab was to examine

different types of animal behavior. In the first half of the lab, the behavior of pillbugs was studied, in particular with regard to kinesis, which is a random movement as a result of a stimulus. First, the pillbugs’ responses to moisture were examined. It was hypothesized that if the pillbugs could choose to settle in a chamber that was moist or a chamber that was dry, then more pillbugs would prefer the moist chamber. Then, in the independently designed experiment, the pillbugs’ responses to background color of their environment were tested. It was hypothesized that if the pillbugs could choose to settle in a chamber that had a black background or a chamber that had a white background, then more pillbugs would prefer the dark chamber. In the second half of the lab, the aggressive behavior of the Siamese fighting fish (also known as Betta fish) was examined. In particular, the effect of laser pointer light on the behavior of the Betta fish was investigated by counting the rate of movement of the fish’s pectoral fins. It was hypothesized that if a laser pointer was shined on the fish, he would move his pectoral fins at a faster rate compared to his usual rate of movement of pectoral fins. The types of behaviors investigated in this lab include taxis and kinesis. Whereas the animal moves either toward or away from a certain stimulus in taxis, the animal moves randomly in response to a stimulus in kinesis. Another type of behavior that this lab involves is agonistic behavior, which refers an animal showing aggressive or submissive behavior in response to a given stimulus. Materials  10 pillbugs
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A sketch of a pillbug was produced. A petri dish cover was placed on the dish to prevent the pillbugs from climbing out. A piece of paper towel was wetted and placed in one chamber. movements about the dish. extensive notes were taken of the pillbugs’ general appearance. 5. A choice chamber consisting of two petri dishes joined together was obtained. the number of pillbugs on each side of the choice chamber was counted and recorded. 6. 10 pillbugs were obtained and placed on the wet paper towel in a petri dish.             Mirror Ruler 2 petri dishes joined together Petri dish cover Paper towel Water Black construction paper White paper Timer 1 male Betta fish 1 laser pointer 1 Thermometer A pair of Scissors Procedure Part 11A: 1. 3. and interactions with each other. Any behaviors that involved 2 or more pillbugs were noted. For 10 minutes. while a dry piece of paper towel was placed in the other chamber. Every 30 seconds for 10 minutes. 2 . The number of pillbugs in the wet chamber and the number in the dry chamber were graphed. 7. 5 pillbugs were placed in each chamber. 2. 4.

pH. 13. The controlled variables for the independently designed part included temperature. Organism/Apparatus – the 10 pillbugs (also known as sowbugs) 3 . size of each petri dish. Both pieces of paper were wetted and placed in each of the chambers. Every 30 seconds for 10 minutes. Controlled variables – The controlled variables for the general part included light. The independent variable for the independently designed part was the background color of the petri dish. etc. 11.8. the number of pillbugs on each side of the choice chamber was counted and recorded. The dependent variable for the independently designed part was the number of pillbugs located in the black background chamber versus the number of pillbugs located in the white background chamber. etc. 9. In the independently designed part. Dependent variable – The dependent variable for the general part was the number of pillbugs located in the wet chamber versus the number of pillbugs located in the dry chamber. light. moisture. 12. Independent variable – The independent variable for the general part was the moisture of the petri dish as altered by whether the paper towel was wet or dry. The pillbugs were disposed of and the petri dishes were washed and put back. temperature. 10. 5 pillbugs were placed in each chamber. a circle the size of a petri dish was cut out from white paper. A circle of the same size was cut out from black construction paper. whether it was black or white. size of each petri dish. The number of pillbugs in the black background chamber and the number in the white background chamber were graphed.

7. A male Siamese fighting fish was obtained in a plastic container. The fish’s behavior when he noticed the mirror was described. Physical factors such as water temperature were also noted. 6. light. was developed into a proposal to test how it affected the aggressive behavior of the Betta fish. Also. All the data were converted to rate of movement per minute. As a group. 5. A mirror was lowered into the plastic container and left there for 10 minutes. The male Betta was observed. 8. 4. The average for the control trials and the average for the experimental trials were computed and compared to examine whether and how the stimulus of light affected the behavior of Siamese fighting fish. several variables that would either increase or decrease the frequency of aggressive behavior in Betta fish were brainstormed. The rate of his pectoral fins movement was recorded for each 30-second period. 4 . the purpose of the upward pointed mouth was noted and speculated. Three experimental trials were performed in which a laser pointer was pointed from the top to the fish. Before testing this variable. including his aggressive and submissive behaviors. 3.Part 11B: 1. three control trials were conducted by counting the number of times the fish moved his pectoral fins in a 30-second period under regular conditions without additional light. 9. including whether there were a collection of bubbles floating at the surface of the tank. 2. The behavior of the Betta fish when he was alone in the tank was recorded in detail. One of these ideas.

0 3. Controlled variables – Temperature.0 6.0 5.5 5. humidity. Dependent variable – the dependent variable is the rate of movement of the Betta fish’s pectoral fins.5 7.5 3.0 7.5 8.0 2.5 4.0 1. pillbugs hide under paper towel One pillbug climbed onto the top of the paper towel One pillbug chose to move to wet chamber One pillbug moved to wet 5 .Independent variable – The independent variable was the presence of additional light in the form of the laser pointer to the environment of the Betta fish.0 Number in wet chamber 5 7 7 7 7 7 7 7 8 8 8 8 8 8 8 8 9 Number in dry chamber 5 3 3 3 3 3 3 3 2 2 2 2 2 2 2 2 1 Notes Equal number of pillbugs in each chamber to begin with In dry chamber. noise Organisms/Apparatus – one male Betta Fish Results Part 11A: Pillbug Movement over Time Between wet and dry chambers Time (min) 0 0.0 4.5 6.5 1.5 2.

0 Number in black background chamber 5 4 4 4 4 4 4 4 2 2 2 Number in white background chamber 5 6 6 6 6 6 6 6 8 8 8 6 .5 3.0 9. the rest all proceeded to reside in the wet chamber.5 2. it is evident that as time went on.0 4.5 10.0 2.) Wet chamber Dry chamber Description: From the above table and graph.0 1.0 9 9 9 9 1 1 1 1 Pillbug Movement over Time Between wet and dry chamber number of pillbug in chamber 10 8 6 4 2 0 0 2 4 6 8 10 time (min.5 4.5 5.5 9. more pillbugs chose to settle in the wet chamber.5 1. only one pillbug was left in the dry chamber.0 3.chamber 8. Pillbug Movement over Time Between black and white chambers Time (min) 0 0. By the end of the 10-minute period.

nine pillbugs were found to be in the white background chamber whereas only one pillbug was seen in the black background chamber.0 2 2 2 2 1 1 1 1 1 1 8 8 8 8 9 9 9 9 9 9 number of pillbug in chamber Pillbug Movement over Time Between black and white chamber 10 8 6 4 2 0 0 5 time (min. By the end of the 10-minute period. as time went on.5 6.5 7.0 9.5 8.0 7.5. Part 11B: Effect of light on pectoral fins movement rate Pectoral fins movement rate Trial 2 Trial 3 Trial 1 Average 7 .5 10.0 6.5 9.0 8.) 10 15 black chamber white chamber Description: According to the data table and graph. the number of pillbugs in the white background chamber increased while the number of pillbugs in the black background chamber decreased.

This is due to the fact that it is usually more humid and damp in dark environments. Also. they require a moist environment to breathe. such as moisture. several conclusions can be drawn. This is not surprising because pillbugs use gill-like structures to exchange gases. In the independently designed experiment. the rate of the Betta fish’s pectoral fins movement was roughly 50 times higher per minute under the laser pointer than under ordinary light conditions. Several reasons could account for the experimental error. For the pillbug part. Conclusion Having done this lab. For the Betta fish part of the lab. pillbugs indeed prefer moist and damp conditions. may not have been fully eliminated during the experiment. as shown by the fact that an overwhelming majority of pillbugs chose to move to the wet chamber as opposed to the dry chamber. The rate of the 8 . the data collected were inaccurate since background research show that pillbugs generally prefer dark environment as opposed to light environment. some of the controlled variables. the moisture content on the black and white papers could have differed. instead of moving about randomly. Therefore. it could be concluded that the presence of light from the laser pointer increased the aggressive behavior of the Betta fish.Control Laser pointer light Description: According to the above table. The pillbugs’ response to moisture can best be classified as taxis since the isopod moved toward the stimulus. The sample size might not have been large enough to provide accurate results. One of them could be that the number of pillbugs involved in the experiment was only ten. In other words. the wet chamber.

Therefore. 9 . When the Betta fish realized that a strange light source was coming to him. it responded with aggressive behavior. This was in agreement with what was hypothesized.pectoral fins movement was drastically higher when the fish was shined upon by the laser pointer. This allows the Betta fish to scare off the predator(s) emitting the light and therefore contributes to the long-term evolutionary survival of the fish. as demonstrated with his more rapid movement of his pectoral fins. he probably thought that some predator or harm was on its way.