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Jaine Maragathavally (Vally) Kommineni, PhD

4300 Whitwick place, College Station TX-77845


C: (979) 574-4270, email: jk13256a0@westpost.net
PROFILE: Ph.D. with 12+ years of professional experience
* Excellent experience in Molecular Biology, Biochemistry, and functional genomi
cs.
* Co-ordinated with cross-functional teams with multidisciplinary group of scien
tists to complete relevant project activities in a compliant manner.
* Initiated independently new project concepts as well as project collaborations
.
* Demonstrated ability to communicate effectively to persons with a wide range o
f technical abilities and levels.
* Goal oriented, highly motivated team player
ACADEMIC QUALIFICATIONS:
* PhD (Plant Molecular Physiology) University of Hyderabad, INDIA (1995)
- First identified *-amylase in bundle sheath chloroplast.
- Immunological and in vivo labeling studies revealed that the segment proximal
to the leaf base shows maximum photostimulation of *-amylase.
* MSc (Plant Science), Sri Krishnadevaraya University, INDIA (1986)
* BSc (Biology) Sri Venkateswara University, INDIA (1984)
RESEARCH EXPERIENCE
2007-present Assistant Research Scientist
Molecular Genetics & Biotechnology Laboratory, Texas AgriLife Research, Texas A&
M University
* Designed both viral and nonviral vectors.
* Inserted genes at precise location with fusion proteins. Obtained 67-96% targe
ted transgene integration with non-viral vectors and increased gene integration
by 12.7 fold.
* Developed Gene therapy method to introduce therapeutic gene in specified site
on genome. Developed single plasmid constructs combining helper and donor plasmi
ds into one.
* Innovative Adenovirus system is developed with piggyBac transposable element (
~ 43kb DNA) towards site-specific integration with recombineering.
* Modified gene expression by swapping domains between three different proteins.
Analyzed association of domain in protein transportation.
2001 to 2007 Postdoctoral Research Associate Texas AgriLife Research, Texas A&M
University
* Gene expression efficiency analyzed with LexA Gal4, Sp1, GATA Zn finger domain
s in insect system and Gal4 and 3 different Zn finger domains in mammalian syste
ms. (15 projects with fusion proteins).
* Conjugated 3 genes placing linker and nuclear localization signals between gen
es and expressed fusion protein.
* Generated transgenic lines with Zinc finger nuclease technology and recombinas
e systems.
* Created DNA adenine methyltrasferase (DamID) N- and C-terminal fusion with CTC
F to analyze heterochromatin regions and gene insulations.
* Synthesized genes over 5kb with overlap PCRs along with His, HA and Flag tags.
Modified gene expression by swapping domains between proteins. Analyzed associa
tion of domains in protein transportation.
* Developed transgenics against infectious diseases.
1999 to 2001 Departmental Postdoctoral Research Associate & CSIR Pool officer,
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangal
ore, India
* Engineered edible vaccines for Rinderpest and Peste des Petits Ruminants (PPRV
) viral diseases.
* Expressed biologically active vaccines.
* Quantified protein expression with ELISA
* Engineered viral resistant systems.
1995 Jan -1996 Dec CSIR Research Associate/ Production Associate (Industrial Exp
erience)
Southern Petroleum Industrial Corporation (SPIC), Chennai, INDIA.
* Standardized large-scale production of Horseradish Peroxidase.
* Obtained 3240U total peroxidase activity from small tissue in 60 days, with ob
tained purity and specific activity, enzyme can be used directly in diagnostic k
its.
* Purified amylase, phosphorylase and peroxidase enzymes. Hands on experience in
scale up studies.
* Transfer methods and protocols to technology transfer groups.
HONORS & AWARDS
* Organizing/Program Committee member, In Vitro Biology Meeting, USA 2004, 2
005
* Outstanding Member Award, by Society of In Vitro Biology, USA 2004
* Chair, Development committee, for 11th International Conference on Invertebrat
e Cell and Tissue culture, San Francisco, CA, USA 2004
* 'IAPTC&B fellowship' award, Orlando, Florida, USA 2002
* CSIR pool officer fellowship, India 2001; CSIR/UGC Research Associate Fellow
ship, India 1989-1996
* Student travel award by CSIR, India to Singapore (2000) & to CSH meeting USA (
1995)


PROFESSIONAL SKILLS:
* Extensive experience in molecular biological techniques including cDNA library
construction, codon optimization, cDNA and genomic cloning, sub-cloning, Northe
rn and Southern analysis, DNA sequencing, PCR, qPCR, RT-PCR, inverse PCR, 5'& 3
' RACE and site-directed mutagenesis. Excellent experience with Adopter ligated-
PCR
- Ability to create enzymes sites in gene without changing ORF.
- Redesigned vectors, rewritten ORF basing on codon bias and improved gene expre
ssion.
* Recombinant DNA vector construction for plant, bacterial and mammalian express
ion, promoter analysis in transgenic cell lines, gene expression, Gel mobility s
hift assay, Radioactive labeling and detection. Analytical techniques such as en
zyme assay, quantifications, kinetic study, TLC and HPLC.
* Excellent experience in fusion protein and chromatin modifying proteins constr
uction; Recombinant protein expression and purification, SDS-PAGE, 2D gel electr
ophoresis, Immuno-electrophoresis, Chip assay, Immunochemical assays, In vivo la
beling, In vitro translation polyclonal antibody production, Western analysis, E
LISA, plant tissue culture and plant transformation.
* Viral vectors construction, Recombineering, viral propagation, infection, vira
l stocks preparation.
* Mammalian (HEK293, CHOK, U20S, Mice Fibroblast cell lines) and insect (ATC10,
C710, Sua4, S2 cell lines) cell culture maintenance, transfections. RNA interfer
ence collaborated with mice transgenesis.
SUPERVISORY SKILLS:
Guided and trained undergraduate, graduate students, integrated PhD student, res
earch associates and medical professionals from TAMU and other Universities (200
1 to present).
Guided two undergraduates from Kingsville Texas A&M University in NSF funded REU
-EXCITE
Program (2008); PhD student from Kentucky University (2004) and Little Rock, AR
(2003, 2005)
Trained students in plant, insect and cell transformations; vector construction
and enzyme analysis.
COMPUTER SKILLS:
Vector NTI version 11 for gene data mining, sequence analysis, primer and plasmi
d designing.
Familiar with NCBI Gene Bank search, Entrez, Blast, and Zinc finger tools. Knowl
edge and experience with MS Word, PowerPoint, Excel, Adobe Photoshop, and Dream
weaver.
TEXAS A&M UNIVERSITY'S LEARNING & DEVELOPMENT COURSES:
Craft of Grant writing: Semester long course from office of Proposal Development
, TAMU 2007
Basic mice handling techniques, TAMU 2002; Organization skills, time management,
leadership skills.
IMMIGRATION STATUS: Permanent Resident.
PUBLICATIONS : Journal Articles (9 + 3) ; Book chapters (4) Conference presentat
ions (17)