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NAME:

KEV

Exam2

Bubblein your answersheetwith the correct choicein eachcase(45points)

1.Onthegraphsbelow,whichlinerepresentstheadditionof aNON-COMPETITIVE INHIBITOR to theoriginalcondition(line 1)?

2. Whichstatementis NOT trueof thesequentialmodelof allostericbinding?

a. a subunitcanexistin eithertenseor relaxedformsdependingon the circumstances

b. bindingof aninhibitor to onesubunitmakesbindingof inhibitor to othersubunits

easier.but doesnot fullv converttheothersubunitsto thetenseform

-.A'

f

Yut

d.

e.

c) bindingof activatorto onesubunitmakesbindingof activatorto othersubunitseasier,

doesnotaffectsubstratebindingin anyof theunits

activatorandsubstratebindingmaybesaidto oostabilize" therelaxedform

inhibitorbindingmaybesaidto oostabilize" thetenseform

3. Whichof thefollowingismostlikelyto bephosphorylated?

(R: (CHz)NHr*)

ALys (b)Ser (R : CH2OH)

E cly (R: H)

4. Whichis ansaturatedfattyacid?

a. oieicacid:18:l-Ae

b. linoleicacid;18:2-Ae'12

c. linolenicacid;18:3-Ae' 12'15

d. Ala(R: CH3)

e. theyareall equallylikely to be

phosphorylated

d. arachidonicacid;20:4-A5'8' rr'14 norr.of theabove

6)

\J

5.

Whichvitaminis biosynthesizedfrom cholestelolvia UV light?

vitaminA

a.

b.

c. vitaminC

vitaminB

@vitamin

D

e. vitaminE

6.

a.

b.

c.

([glu]"*> [glu]'")

d. glucoseis co-transportedinto a cellvia aNa-/glusymporter([glu]*t < [glu]in, and

Which is anexampleof primary activetransport?

COzmovesacrossthemembraneoutof a cell

Ozmovesacrossthemembraneinto a cell

glucosemoveswith theassistanceof themembraneproteinpermeaseinto a cell

([Na-]ou,

> [Na-]in)

@Nu* is transportedoutof a cell via Na*/K*-ATPase

7. Which of the diagramsbelow representsD-glucose?

a.

cHo

|

I

b.

qHo

|

C

-

gHo

I

H-oH

n--|--onn-forlr,#t

Ho-tH

HtoH

Hotn
I

n*on

no*n

n-*-oH

n**

no*n

r-*-or

lllll

CH2OH

CH2OH

CH2OH

d.

-

cHo

i-

H--Fon

Ho-l-r-r

no*n

,-J-on

CHrOH

8. Whichof thefollowingactivatesphosphorylasekinase?

a. cAMP

b, glycogenphosphorylase

-

@

proteinkinase

e. UDP-glucose

e.

cHo

|

H-1-oH

Hc

I

H-J-oH

no*"

CH2OH

c.

glycogensynthase

9.

In thedisaccharidecx-lactose(glycosidiclinkageis galactose-B-1,4-glucose),whichof

thefollowing isll1ftl trae? a" thegalactoseis in theo position

@m.

glucoseis in thecrposition

i. theanomericin thecrpositionis

lockedintoplace

d. neithermonosaccharidecan muiargtatg e- bothmonosaccharidescanmutarotate

10.Whichspeciesis theelectondonorin thereactionshown:

NAD* + CH:CHzOH-r NADH + H* + CH3CHO

a.CH:CHO

d. NAD*

b. NADH

e. insufficientinformation

@urcHroH

BCH 361 Hendrickson

11.Whichgeneralstatementis TRUEregardingmetabolism?

a. catabolismis thesumof metabolicandanabolicreactions

b. anabolismis generallya reductiveprocesswhichreleasesenergy

c. catabolismis generallya reductiveprocesswhich requiresenergy

@catabolism

is generallyanoxidativeprocesswhich releasesenergy

e. anabolismis generallyanoxidativeprocesswhichrequiresenergy

12.Fora systemat equilibrium,whichof thefollowingMUST betrue?

a.AG>0

b.aG<0

@lc: o

d.AGo'= 0 e. AG"' < 0

13.Theformationof whichintermediateoccurswith a large,negativeAG in glycolysis?

a. fructose-6-phosphate

(6) glucose-6-phosphate

i

phosphoenolpyruvate

d, 3-phosphoglycerate e. glyceraldehyde-3-phosphate

14.Whichis NOT aproductof thenetreactionof glycolysis?

a. 2ATP

b.

c. 2 pyruvate

2 NADH

@itu"tut"

Y

ail of theseareproducts

15.WhichdoesNOT a typeof regulationof phosphofructokinase?

d. activatedby AMP

a. activationby fructose-6-phosphate

b. inhibitedby citrate

c. inhibitedby ATP

O "ll oftheseareaccurate

Answer.thequestionsbelow;showcalculationswhererelevantfor partial credit

1.(10points)Givengalactose(below),drawc-lactose(galactose-B-1,4-glucose).

BCH361 Hendrickson

2. (15points)GiventheLineweaver-Burkplot shownbelow,calculateKm andVmax

for eachcondition.Indicatewhethertheinhibitoris competitiveor non-competitive. Sketcha Michaelis-Mentonplot thatcorrespondsto thedata. (Note: yourMM plot does not needto includenumbers;simply indicateKm andVmax, andgetthe basicshapeof theplot correct).Labelall axes.

o(t

---

G

Vnr.*'

K^

lN lhg

Inhibitor

ntercept---5.31

t/y-,nt --

-l/.-'

t =

8,lq r to -' -',/,o,Lq

:

Vuar = 8.11xlo-L

Lr^'

-l/-s.st =

I cr*Pennvt

l.81x lo -r

ffrt tou.

V

( ,01,

q)

x to-L (,u0,? A

1.7L

(o -ttt\

o?-ro

1rul".rb

K^

D^S)

K^(t"t",5)

fsl

BCH 361 Hendrickson

3. (15points)ATP canbeusedto phosphorylateglucoseto makeglucose-6-phosphate. Whatis thenetreaction?Whatis AGo'for the reaction,glventhe following:

glucose+ Pi + glucose-6-phosphate,AGo'= 13.8kJ/mol ADP+ Pi+ ATP,AGo':30.5kJ/mol

R=8.314JlmolK.T=298K

'

AT? t SLr,tcos4

bGl

.i;t:

"r'

rrn,'

o;*;*

l3.t

>

.5l*l

-

3o:5 LS/,r,toL '

-16'+

lc1l"+ol

BCH361 Hendrickson

4. (15points) Fill in theblankintermediatesbelow. ADDITIONALLY:

l)

Providethenamesof thethreemajorenzrymes,andclearlyindicatewhichstep

2)

they catalyze Indicatethe step(s)whereATP is required,andhow manyATP arerequiredPER

3;

GLUCOSE Indicatethestep(s)whereATP is produced,andhowmanyATP areproduced PERGLUCOSE

4)

Indicatethe step(s)whereNADH is produced,andhow manyNADH are producedPERGLUCOSE

Lrrt,,)

AT

)

PN /

[tr*>

AtP --

nNP/

gluc.'sc

I

f;("P

t

FaP

r

i

Vvroutnftoa-

prn

lhrctose-I .6-bisphosphatc

bHrflp

|

\.

lal*t^)

v

v

/\

-----+

A,5?

Pil)r'

-

)

trt*A Hl

grrccrartrcrrrdc-.1-ph.r'h.rr. (

I

Y

L>

NA,S*

^JAs\1

'

GlYcua{+-\ '3'btsPho.S

|

+

-phosplxrglvccratc

I

!-phosplroglrccrrrtc

t

I

phosphocnolpr'rur'atc

i

pu.Linnr*e

PYnrvruK

Gr*,-)