This action might not be possible to undo. Are you sure you want to continue?

-Dec, 1997

**Reliable criteria for the selection of broodstock of Penaeus monodon Fabricius in genetic improvement programmes
**

p. KISHORE CHANDRA, K.K. VIJAYAN AND L.H. RAO Central Institute of Brackishwater K.V. R A J E N D R A N , Aquaculture, T.C. SANTIAGO, India

Chennai - 600 008,

ABSTRACT Level of variation in tail weight and its degree of dependence on different morphometric traits were estimated in Penaeus monodon. The data on morphometric traits of a total of 165 P. monodon of four different weight groups, were subjected to stepwise regression analysis. The coefficient of variation of tail weight in all the groups was found to be higher. Correlation matrices revealed a significant relationship between the morphometric traits and tail weight, particularly in the broodstock animals. The order in which different morphometric traits entered the regression equation differed among the wieght groups. In broodstock animals, partial carapace length and carapace width determine the tail weight with high degree of accuracy {R^= 99%). Therefore, these easily measurable traits can be used as criteria in the selection of P. monodon broodstock. Introduction Penaeid shrimp culture has attained a commercial status in India and hence there is great demand for seed. Large . scale seed production depends on t h e availability of wild broodstock. However, wild spawners are in high demand in all shrimp farming countries. T h e problem is more acute with Penaeus monodon as compared to the other species. As ofnow, pond reared broodstock play a minor role in the spawning programmes of hatcheries (Alagarswami, 1993). So the captive broodstock development and selective breeding are the major areas through which genetic improvement a n d assured supply of ^0°^ quality seed could be achieved, Growth rate is a primary trait for selection in most organisms (Shultz, 1986). In general, body weight shows a very high phenotypic variation in all species of fish a n d shellfish. Due to their high fecundity i t is possible to apply a very high selection intensity (Gjedrem, 1983). Therefore, even at low heritabilities t h e potential for genetic improvement of growth rate through selection is promising in aquatic organisms. Further the success of a selection programme depends on the knowledge of base Hne data on quantitative varia-

18 7. (1986) have studied the morphometric t r a i t s in Peneaus stylirostris.19 16.81 20. 1994) and its correlation with easily measurable morphometric traits. 10 .29 Tail weight (TW) (gl1 2.40 10.26 0.07 0. In the present study.80 30.21 0.08 0.10 0. B.13 0.65 15.20 g.60 68.57 . 332 tion of t h e key economic trait (Hallerman.30 g and D > 60g (broodstock).05 30.68 0. Materials a n d m e t h o d s A total of 165 P. Nine morphometric traits (which could be measured without bruising the animal) showing a possible correlation with tail weight.21 0. flexed (FLF) Sixth segment length (SSL) Posterior abdomen circumference (PAC) Sixth segment depth (SSD) 18. Kishore Chandra et al.68 13. The weight groups considered were: A.21 29. monodon of different sizes collected from Chennai coast formed the material for the study.77 10.63 0. monodon Morphometric traits (mm) Weight group A N= :67 Mean CV Weight group B N=57 Mean CV Weight group C N=21 Mean CV Weight group D N==20 Mean CV Partial carapace length ( P C D Carapace width (CW) First segment length (FSL) Anterior abdomen circumference (AAC) Second abdominal segment depth (SAD) Fifth segment length.28 6.05 18.61 0. 20 .28 0.10 0. vannamei and P.18 0.70 0.00 0.44 13.81 16. Earlier Lester (1983) and Goswami et al.23 0.21 15.08 83.12 34.21 18.04 0.86 34.13 0.90 25.12 34.90 0. preliminary information on the level and organisation of variation in tail weight and its correlation with different morphometric traits of P.80 0.43 7.21 0. TABLE 1.97 0.43 0.24 38.10 0.09 0.61 20.52 15.07 0.25 45.85 0. monodon has been analysed and the possible use of these traits in selecting the broodstock is suggested.95 20.07 58.p.42 10.80 26. Means of morphometric traits along with coefficients of variation in different weight groups of P.15 0.81 12. were identified (Lester.08 0.09 0. C.10 0.61 16.10 0.79 0.90 60. 1983) and are Msted in Table 1.11 0.09 0.16 9.08 0.19 0.09 0. These shrimps were divided into four different weight groups based on the total weight. merguiensis and derived prediction equations for tail weight.09 9.09 0. P. 0 .09 54.10 26.10 g.16 0.

vannamei.82 0. monodon.86 0.84 0.84 0.95 between TW and BSD in P.78 0.83 0. Coefficient of variation of tail weight are found to be almost twice that of their respective linear measures except in weight group C.84 0.86 0. TW showed the highest correlation of 0.84 1.00 0. It is important to note t h a t the correlations are higher in weight groups A and D (Table 2) and relatively low in weight groups B and C (Table 3).76 0.90 0.86 0.80 0.76 0.88 0. stylirostris and 0. 1967).93 0.80 0.91 0.88 0.88 AAC 0.98 0.87 0.93 PAC 0.88 0.86 0.90 0. (1986) also reported high correlations.92 0.80 0. Results and discussion The means along with coefficients of variation of different morphometric traits in various weight groups are presented in the Table 1. 1986).93 0. 2 Matrix of correlation coefficients among the morphometric traits.80 0.91 0.83 0.83 0.89 0.80 0.84 0.90 FLF 0.Criteria for selection of broodstock for genetic improvement 333 The data were subjected to stepwise regression analysis by using computer software .88 1.91 0.93 SAD 0.00 0.91 1.00 0. in the same weight group.00 0.86 0.78 0.67 0.94 0. Coefficients of variation for different morphometric traits are higher in the weight group A when compared to the other weight groups.85 SSL 0..75 0. Weight group D values are given above the diagonal and weight group A values are given below the diagonal Weight group D PCL PCL CW FSL AAC Weight group A SAD FLF SSL PAC SSD TW 1.84 0.79 1.85 0.83 0.72 0. This TABLE. Lester (1983) and Goswami et al. In weight group A.90 between TW and PCL in P.92 0.91 0.81 1.80 0. implies t h a t the tail weight is highly variable and so can be exploited by selection depending upon the amount of additive genetic variance of the total variance.76 0.90 0.75 1.86 0.86 1.91 0.00 0.90 CW 0.68 1. So the present study revealed t h a t in P. stylirostris.00 0.80 0.93 SSD 0. tail weight is highly correlated with the morphometric traits both in juveniles (A) and in the broodstock (D).81 0.92 0.87 0.88 0.90 TW 0.91 0. influence of sex was not considered as it was reported earlier t h a t sex has no significant effect in the regression analysis (Goswami et al.82 0. vannamei and P. However.97 0. Lester (1983) reported the highest correlation of 0.00 0.88 0.85 0.84 0.86 0. PAC and SSL.00 0.93 with AAC.00 . Correlation matrices have shown t h a t most of the morphometric traits are having significant relationship with tail weight in different weight groups studied.Microstat and the statistical significance of relationships was estimated (Snedecor and Cochran.84 0.92 0. Lester (1983) also observed a similar p a t t e r n in P.87 0.93 0.85 FSL 0. These variations may be due to the species differences. While analysing the data.87 0.93 1.84 0.00 0.78 0.80 0.89 0.92 0. The influence due to geographical location was removed by collecting the animals from the same agro-climatic zone.

07 0.33 0.60 0.63 0.59 0.61 0.13 0.00 0. In the weight group A.60 0.17 0.59 0. The addition of two more variables raised the Revalue to its maximum (94 %). 88 % of the total variance of the tail weight could be explained by anterior abodmen circumference (AAC) alone.19 1.17 0.30 0.80 PAC 0. In the weight groups A and D.35 0.06 0.25 1.48 1.19 0.66 0. The order in which different morphometric traits entered the regression equation in different weight groups stepwise regression Weight groups A AAC SAD PAC SSL PCL FSL FLF SSD CW R^ (%) 94 B SSL PCL AAC CW SSD SAD FLF PAC FSL 89 C CW AAC SSL FLF SAD PCL PAC BSD FSL 85 D PCL CW SSL SAD SSD FLF FSL AAC PAC 99 .67 0.24 1.01 0. the total TABLE 4.34 1.17 -0.40 1.63 0.22 1.62 0.29 0.79 TW 0.38 0.07 0. In case of broodstock (weight group D) partial carapace length (PCL) alone explained 96 % of the total variance of the tail weight. The results have revealed that various morphometric traits entered the regression euqation in different orders (Table 4).02 0. after which the further addition of variables could not contribute in explaining the variance in tail weight. Kishore Chandra et al. In all the weight groups.59 0.31 0.09 0. With the addition of one more variable (carapace width.41 -0.55 0. CW) to the equation.33 0. 334 TABLE 3.53 0.00 0.52 0.52 0.34 0.00 0.20 0.66 0.75 0.06 0.66 0.74 SAD 0.04 0.76 BSD 0.63 0.60 0.63 0.64 0.69 0. R^ values are high when compared to weight groups B and C.52 0.28 0.00 0.p.00 the reason for the lower correlations in weight groups B and C is to be further ascertained through adequate data.64 0.61 SSL 0. indicating a higher degree of relationship in the former weight groups.64 0.57 0.68 FLF -0.78 FSL 0.46 0. Weight group C values are given above the diagonal and weight group B values are given below the diagonal Weight group C PCL PCL CW FSL AAC Weight group B SAD FLF SSL PAC BSD TW 1.57 0.50 0.34 0.51 0.68 0.00 0.80 CW 0.59 0.52 0.07 1.68 0. the coeffficient of determination. Matrix of correlation coefficients among the morphometric traits.13 0.45 1.69 0.00 0. The stepwise regression analysis was done with tail weight as the dependent variable and all the other morphometric traits as independent variables.00 0.61 0.22 0. regression is highly significant indicating that the tail weight is highly influenced by the morphometric traits.00 0.70 0.74 AAC 0.42 -0.67 0.31 0.00 0.41 0.48 0. This is in accordance with the observation made regarding the correlation coefficients.63 0.68 0.

Planning for augmenting shrimp seed production.67 PCL TW = -87. Alagarswami. Therefore the morphometric traits such as PCL and CW which are highly correlated with the tail weight and easily measurable without actually sacrificing the animals. Rao.72* 6. . possibility of improvement by genetic selection. K. Fishing Chimes. for the facilties provided and the encouragement given during the study.82 CW TW = -8.39* 98.77 + 0.69 + 0.1 SSL TW = -13. Chennai. Genetic variation in quantitative traits and selective breeding in fish and shellfish.97 + 1.83 + 0.78 + 1. The data have also indicated the use of morphometric traits in predicting the tail weight with high degree of accuracy. 33 : 51-72. However. In juveniles. monodon based on their individuals merit over the population means. Prediction equations for tail weight in different weight groups — stepwise regression R2 (%) Weight group Prediction equation TW =-3.66 PCL Full model regression F value 459. References Alagarswami.M.21* 559. suggesting the TABLE 5. G.40 PCL + 0. 1983.16 AAC Full model regression TW = -8.31* 20.24 SAD + 0. AAC and SAD give an indication about the future growth potential of the animal.R. 1993. T.66* 103.43* 333. Aquaculture.Criteria for selection of broodstock'for genetic improvement 335 variance explained by regression equation reached its maximum of 99 %.94* 15.95 CW + 1.58* • 135. 13(1) : 27-31.81* 93. This is an important factor in the captive broodstock development as it would help the breeders to cull the animals even at the Acknowledgments The authros are highly indebted to Dr. with larger data on broodstock animals to arrive at the population means of the morphometric traits so as to select the broodstock of P.12* 42. Central Institute of Brackishwater Aquaculture.71 CW Full model regression TW = -86.69 SSL Full model regression TW = 1.96* 489. monodon.21 AAC + 0.62 + 0. early stages of life cycle. further study is warranted.02 + 0.23 AAC TW =-3. with the increment due to carapace width being only 3 % (Table 5). K. monodon for producing seed with faster growth potential.35 + 2. Director. The present study has shown t h a t the tail weight h a s high coefficient of variation in P.01* 88 91 94 65 78 89 45 70 85 96 99 99 * Significant at 1% level. Gjedrem. former Director and Dr. could be used while selecting the broodstock of P.

G. Cochran 1967. F. 1983. 6th Edn. 1986. G.p. 336 Lester. Soc.. Toward coordination and funding of long term genetic improvement programs for striped and hybrid bass Marone sp. Press.. Snedecor. Dalai and S. Shultz.C. Goswami 1986. 25 (3) : 360-365. L. Goswami. for selection of broodstock in genetic improvement programmes. 57 : 65-76. Iowa. and W. . Developing a commercial breeding program. Aquaculturc.M. E. Kishore Chandra et al. Aquaculture. 53 : 41-58. 33 : 41-50. Statistical Methods. Hallerman. Developing a selective breeding program for penaeid shrimp mariculture.W. Iowa State Univ. Aquaculture. 593 pp. Peneaus merguiensis.T. Preliminary studies on prawn. 1994. World Aquacul. U. S.J.G.

Sign up to vote on this title

UsefulNot useful- ESTIMATION OF REGRESSION COEFFICIENTS USING GEOMETRIC MEAN OF SQUARED ERROR FOR SINGLE INDEX LINEAR REGRESSION MODby Adam Hansen
- businessby Lan Anh
- Solution Econometric Chapter 10 Regression Panel Databy dumuk473
- Adoption Of The Prepaid Electricity Meter Billing System By Harare Residents: Was There Some Preference To Conventional Meters?by IOSRjournal

- Factors of Employee Motivatio1by Hemanga Pathak
- Tretnatoinby iltdf
- Psychological Stress and Its Relationship With Competitive Sport Anxiety and Its Rapport With the Achievement Motivation Among Hand Ball Players (Male)by The Swedish Journal of Scientific Research (SJSR) ISSN: 2001-9211
- gplc_apr23by Chunyi Wang

- Repro Duks i
- 6.23 Regression Analysis
- Correlation Analysis
- Sign changes in QSAR
- Predicting Student Proficiency Test Scores
- March 2009
- Risk and Return Note 1
- 1860-7332-1-PB
- article_24_vol_2_3
- Syllabus.0405322.E.statistics
- Solon Et Al 2009 - Reelection
- Intro
- Analysis 1
- Formulae Sheet 2013 Regression
- Where Did Growth Go
- Ch13 Forecasting
- ESTIMATION OF REGRESSION COEFFICIENTS USING GEOMETRIC MEAN OF SQUARED ERROR FOR SINGLE INDEX LINEAR REGRESSION MOD
- business
- Solution Econometric Chapter 10 Regression Panel Data
- Adoption Of The Prepaid Electricity Meter Billing System By Harare Residents
- Factors of Employee Motivatio1
- Tretnatoin
- Psychological Stress and Its Relationship With Competitive Sport Anxiety and Its Rapport With the Achievement Motivation Among Hand Ball Players (Male)
- gplc_apr23
- 2007 ACM WIC Website Comprehensibility
- LISREL Overview
- Machine Learning Revision Notes
- THTR
- Applying Value Added Intellectual Coefficientness to Businesses
- 2014GV903-7-FY
- 8816-19063-1-SM

Are you sure?

This action might not be possible to undo. Are you sure you want to continue?

We've moved you to where you read on your other device.

Get the full title to continue

Get the full title to continue reading from where you left off, or restart the preview.