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# Biology teacher support material Investigation 1

Water Potential of Potato and Sweet Potato; the Weighting Method.
Research Question: How will the weight vary between the sweet potatoes and potatoes when placing each sample in different concentration solutions? Hypothesis: When placing samples in hypertonic solutions they will loose weight. And on the other hand when they are going to be placed in hypo tonic they will gain weight. But the difference in weight will vary in potato and sweet potato since the sweet potato tastes sweeter and it has a higher sugar solution. Variables: Dependent: - Percentage % (difference in weight) Independent: - Initial Weight of Potato (grams) - Final Weight of Potato (grams) Controlled: - Molarity (0,0; 0,25; 0,50; 0,75; 1.0) Volume of the solution to submerge the potato Potato cylinder size Room temperature (19°C.) Materials: Big Potato Cork Borer Solutions of water and sugar (Molarities: 0,0; 0,25; 0,50; 0,75; l,0) Blotting Paper Electronic Balance Test Tubes Test Tube Racks Procedure: l) Five test tubes were labelled, each one with the different molarity of the solutions: (0,0; 0,25; 0,50; 0,75; 1,0) 2) The tubes were filled with the different concentration solutions, according to what was previously marked, up to three quarters of the tube. 3) With a cork borer five potato cylinders were cut and placed over five different 'blotting papers that were labelled with the 5 different concentration solutions randomly. 4) The potato cylinders were dry up and weighed on an electronic balance and then placed in the test tube with the solution according to the concentration describes at the blotting paper. 5) All the initial data was recorded on a previously prepared table. 6) The samples were left for a day in the test tube racks so the process took place. 7) The next day the potato cylinders were taken from the test tubes and placed on blotting paper labelled with its respective molarity. 8) The cylinders were weighed and the final data was recorded on the table. 9) The steps were repeated but instead of using potato, this was replaced by sweet potato. 10) Calculations and graphs were prepared. 11) Conclusions were drawn to prove the hypothesis.

© International Baccalaureate Organization 2007

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5 0.2 0.0 1.1g 0.9 Difference in weight (grams) ±0.7 0.8 1 Sweet 1.3 1.8 0.45M = -1.1g 0 0.4 0.0 1.9 1.3 -0.Biology teacher support material Investigation 1 Data collection: Potato in different concentration solutions.1g 1.1g 0 0.1 -0.6 Percentage % (difference in weight) 44 33 -30 -20 -54 DCP 2/3 Sweet potato in different concentration solutions Molarity Initial weight (grams) ±0.50 0.3kpa potato Potato " 2 © International Baccalaureate Organization 2007 .2 -0.3 0.1 0.00 0.0kpa 0.75 1.25 0.0 1.75 1.4 1.2 1.4 0.1 Final weight (grams) ±0.6 0.2 0.4 0.9 0.1g 0.1 Final weight (grams) ±0.2 0. DCP 1 Molarity Initial weight (grams) ±0.3 -0.50 0.1g 1.00 0.8 0.8 0.2 Percentage % (difference in weight) 50 37 55 10 -18 Graph: Potato and Sweet Potato Solute Potential DCP 2/3 80 Percentage (%) of difference in weight 60 40 20 0 0 -20 -40 -60 -80 Molarity 0.1 1.9 1.1M = -3.25 0.6 Difference in weight (grams) ±0.

3 kPa and the sweet potato -3. that is when substances move from a place of low concentration to a place of a higher concentration through a semi permeable membrane. so that more samples are used. of course that doesn't depend on us that's just because we don't have enough time to dedicate to each experiment. potato gained more weight since the line is moved to the left of the graph. I can state that in my case. by determining my observations my hypothesis was correct because when placing samples in hyper tonic solutions they loosed weight. although we used an electronic balance the weight isn't exact and that may cause marginal errors. This occurred because of the sucrose that the sweet potato contains it will obviously gain more weight in hyper tonic solutions than potato and gain loose weight in hypotonic solutions. As I predicted there was a difference in the resulted weights between the potato and the sweet potato. One of the observations that was clearly pointed out was the exact measurement of potatoes. A possible improvement for this could be to try and be as precise as possible to be exact with measurements.Biology teacher support material Investigation 1 -3CE 1 CE 2 CE CE CE 3 2 2 CE 3 Conclusion: After doing the work and collecting relevant data to know if my hypothesis was right or wrong. The differences in results between potato and sweet potato are because of the sucrose that sweet potatoes contain. but obviously we don't have the possibility of getting more exact balances. Other problem that surged along the experiment was the lack of samples. © International Baccalaureate Organization 2007 3 . Although the experiment resulted successful there are several problems that appeared along the work that could certainly be improved so that the results become more accurate. we could finish the work and obtain reasonable and relevant data to determine how diffusion occurs at hyper and hypotonic solutions according to the sample placed in each. and on the other hand when placing them in hypo tonic ones they gained weight respecting the potato solute potential which is -1. although we've tried to do this as equal as possible with each sample its quite difficult that the samples result dried up all the same. This process basically occurs by osmosis. A possible solution for this problem may be not to dry the samples up and used them just as they are taken from the solution. Other important factor that could have influenced in our work is the way we dry up the samples after extracting them from the solutions.0 kPa. But besides of the observations that I made and the errors that we made.