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490 Series Biological Microscope OPERATOR’S MANUAL READ THIS MANUAL BEFORE USING THE MICROSCOPE Contents 490 SERIES Before use .............................................................................................. 3 1. Nomenclature .................................................................................... 4 2. Operation ............................................................................................ 8 2-1 Assembly ........................................................................... 8 2-2 Setting up .......................................................................... 9 2-3 Focusing ............................................................................. 9 2-4 Adjusting the condenser and diaphragm ............. 10 2-5 Using the trinocular port ............................................. 11 2-6 Setting the stage’s stop limit ...................................... 11 2-7 Adjusting focusing tension .........................................12 2-8 Changing the bulb ......................................................... 12 2-9 Changing the fuse ..........................................................12 13 2-10 Maintenance .................................................................... 3. General speci cations .................................................................... 14 4. Con guration drawing ...................................................................15 5. Optional Accessories .......................................................................16 6. Parameters/technical terms ......................................................... 17 6-1 Objectives ..........................................................................17 6-2 Eyepieces ...........................................................................17 6-3 Electrical system ..............................................................18 6-4 Parameters ........................................................................ 18 6-5 Technical Terms ............................................................... 18 7. Troubleshooting ............................................................................... 20 2 Before use Introduction 490 SERIES Congratulations on the purchase of your new AmScope Microscope! This manual is designed for the B490 and T490 series microscopes. Though both have identical bases, the B490 is equipped with a binocular head (2 eyetubes) and the T490 is equipped with a trinocular head (2 eyetubes & 1 photo/video port). Please take a few minutes to familiarize yourself with the features and functions of your new microscope. If you want more information on microscope, parts, and accessories, please visit our website at: www.AmScope.com We recommend that you study this manual thoroughly before operating the microscope, and that you keep it on hand for future reference. If you have additional questions or need assistance, please send us an email at: info@AmScope.com Please include the microscope model number in your email so that we can identify your model and provide immediate help. Precautions (1) As the microscope is a precision instument, always handle it with care, avoiding impact or abrupt movement during transportation. Do not shake the package. (2) Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry and clean place with temperatures between 32°-100° F (0°-40°C), maximum relative humidity: 85% (3) Avoid touching the lenses on the objective and the eyepiece so that oil and dirt from your ngerprints do not obstruct your view. (4) Before turning the power on, make sure that the power supply voltage is consistent with the voltage of your microscope 3 1. Nomenclature Eyepiece 490 SERIES Binocular Gemel head Diopter Nosepiece Objective Eyetube base Head -lock screw Arm Mechanical Stage Iris adjustment slider Condenser, iris diaphragm Filter holder Base lens Tension knob Coarse focusing knob Fine focusing knob Slide controls Base Dimmer Power switch Fig. 1: B490 4 1. Nomenclature 490 SERIES C-mount Adjustable trinocular port Trinocular Gemel head Limit-stop knob Coarse focusing knob Fine focusing knob Condenser adjustment knob Condenser screw Fig. 2: T490 5 1. Nomenclature Light screw 490 SERIES Fuse Halogen bulb Fig. 3: Bottom Condenser screw Condenser Iris adjustment slider Filter holder Iris Fig. 4: Condenser, iris and lter holder De nitions: Adjustable Trinocular Port Base Lens Coarse Focusing Knob Threaded port with adjustable length for attachment of digital or video camera Directs the light source towards the slide Used to initially bring the slide into sight and focus 6 1. Nomenclature 490 SERIES Condenser & Iris Diaphragm Controls the amount of light that hits the slide Condenser Adjustment Knob Moves the condenser up and down Condenser Screw Screws the condenser in place, secureing it to the mechanical stage Dimmer Controls the amount of light that escapes from the base lens Diopter Allows the focus to be perfected for both eyes, independent of each other Fine Focusing Knob Used to re ne focus to a clear image Head Lock Screw Screws the microscope head into place Limit-Stop Knob Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective (adjustable). Mechanically moves the slide along an X and Y axis for optimal positioning Nosepiece Houses the objective lenses Rotating Gemel Head Adjusts eyepieces to t the distance between the user’s eyes for added comfort Tension Knob Adjusts the tension of the focusing knobs Mechanical Stage 7 2. Operation 2-1 Assembly 490 SERIES (1) First take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which side is labeled up. Remove tape and open the container carefully so as to avoid dropping and damaging the optical items. Check carefully to ensure that all parts and accessories are intact. (2) Check the parking list below: One Microscope Body One Binocular (B490) or Trinocular (T490) Rotating Gemel Head One Adjustable Trinocular Photo Port (for T490 only) Four Objectives: 4X, 10X, 40X, and 100X Two Pairs of Eyepieces: W10X and P16X Color Filters One Bottle of Immersion Oil One 6V/20W Halogen Spare Bulb One Spare Fuse One Dust Cover (3) Remove the microscope body from the box and remove the plastic protective covering. The body of the microscope is composed of the base, the stage, the arm, and the nosepiece. (4) Loosen the metal knob located directly above the nosepiece (the head-lock screw) completely. Place the head, at circular side down, onto the circular opening at the top of the arm. Make sure the microscope head is securely on top of the arm and tighten the head-lock screw to lock the head in place. (5) For the T490 model only, screw the photo port onto the top of the microscope head. (6) Remove the eyetube caps and drop the desired eyepieces into the eyetubes without touching the lens. 8 2. Operation 490 SERIES (7) Screw the objectives into the microscope nosepiece from the lowest magni cation to the highest, being careful not to touch the lens. (8) Plug in the microscope and turn it on. If no light emerges from the light source, adjust the dimmer knob on the side of the base. 2-2 Setting up (1) Loosen the head-lock screw and adjust the microscope head so that it is in a comfortable position for observation. Lock the head-lock screw. With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyetubes and rotating the eyepieces either towards or away from one another, until one circle of light can be seen. (2) Place the specimen to be studied on a glass slide (or simply use a prepared slide), and place it on the stage, holding it sungly in place with the metal slide-holders of the mechanical stage. Using the slide controls, center the specimen over the stage opening, lining it up with the light and objective lens. (3) To adjust the illumination, slowly turn the dimmer until the desired intensity of light is achieved. 2-3 Focusing (1) Turn the nosepiece to choose an objective. It is easiest to use the lowest magni cation rst (usually 4X) to locate and focus the specimen. As you move up in magni cation, you may have to refocus the image a little bit each time. When using the 100X objective, a little immersion oil must be put between the objective and the cover glass on the slide in order to minimize the distortion cause by air. Always wipe the oil o the objective with either a glass-cleaning paper or cloth after each use. 9 2. Operation 490 SERIES (2) Begin focusing by rst looking with one eye through the eyetube without the diopter. Close your other eye. Loosen the limit-stop knob (located on the inside of the coarse-focusing knob) in order to give the mechanicalstage a full range of movement and adjust the coarsefocusing knob to bring the slide into focus. You may also need to re-center the specimen on the stage. (3) When the object is in your eld of vision, adjust the ne-focusing knob until you see a clear, sharp image. BE ALERT: When moving the mechanical stage vertically, be careful not to hit the objective lens with the slide. Remember to use the limit-stop knob to avoid impact between the objective and the slide when using the 40X and 100X objectives. Lock the limit-stop knob at the position where the slide almost touches the objective. (4) Once the image is focused through the one eyepiece, look through the other eyepiece and turn the diopter until the image is clear through that eyepiece as well 2-4 Adjusting the condenser and diaphragm (1) Using the condenser-adjustment knob, you can change the distance between the light condenser and the stage. This allows you to control the concentration of the light hitting your slide. (2) By Changing the aperture of the iris diaphragm, you can adjust the background brightness. Adjust the aperture of the iris diaphragmm using the iris adjustment slider located directly under the stage. (3) If you want to use a color lter, swivel the lter holder out, towards you. You can now place the desired color lter into the circular opening. Slide the lter holder back to original position before observation. 10 2. Operation 2-5 Using the trinocular port 490 SERIES The AmScope T490 is uniquely designed so that you can focus the image through the eyepieces and the trinocular port simultaneously. This “parfocal” feature allows the images through the microscope’s eyepieces, and those displayed on your computer screen or TV to be viewed at the same time! You do not need an adapter to attach your AmScope camera to the trinocular port. However, you may need an adapter if you have a non-AmScope camera. Attaching a camera: (1) If your camera has a C-mount, simply screw the camera onto the trinocular port. (2) If your camera has a 23mm mounting size, remove the C-mount from the top of the trinocular port by loosening the screw. Then, drop the camera into the trinocular tube. (3) To focus through the trinocular port, simply turn the middle portion of the tube. 2-6 Setting the stage’s stop-limit (1) Set the stage to the maximum height desired. (2) Pull the stop-limit knob towards the stage. The stage’s stop-limit is now set, allowing you to move the stage down, but not up past this point. (3) To reset the stage’s stop-limit, simply push the stop-limit knob back towards the back panel of the microscope. The stage’s full range of motion is now restored. 11 2. Operation 2-7 Adjusting focusing tension 490 SERIES (1) To adjust the tension of the focusing knobs, rst locate the black ridged tension-knob on the inside of the coarse focusing knob. (2) To decrease tension, rotate the tension adjustment forward, towards the stage. To increase tension, rotate the tension adjustment backwards, towards the back panel of the microscope. 2-8 Changing the bulb (1) Before changing the light bulb, rst pull the plug out of the electrical socket and wait for the lamp to cool down in order to avoid being burned. Also, make sure to remove the eyepieces or secure them to the microscope before turning the microscope over! (2) Loosen the door-screw on the bottom of the microscope and pull open the small door. Pull the old bulb out and replace it with a new one. Close the door and tighten the screw. 2-9 Changing the fuse (1) First, remove the eyepieces or secure them to the microscope before turning the microscope over. (2) Loosen the door-screw on the bottom of the microscope and pull opUsing a at-head screwdriver, unscrew the fuse located on the bottom of the microscope. (3)Remove the old fuse and replace with a new one. Screw the fuse back in place. 12 2. Operation 2-10 Maintenance 490 SERIES (+) All glass surfaces must always be kept clean. Fine dust on the optical surface should be blown o using a hand blower or gently wiped o with a soft lens tissue. Carefully wipe o oil or ngerprints on lens surfaces using tissue moistened with a small amount of 3:7 alcohol to ether mixture (+) Do not use this solution to wipe the surface of other components. These parts, especially those made of plastic, should be cleaned with a neutral detergent. (+) Do not assemble or disassemble the microscope’s electrical components yourself. (+) After use, cover the microscope with the provided dustcover. Make sure to keep it in a dry and clean place in order to prevent rust. 13 3. Speci cations 490 SERIES Speci cations for the 490 Series Biological Microscope PARTS WF Eyepiece Plan Eyepiece SPECIFICATIONS WF10X/18mm WF10X/18mm with pointer WF10X/18mm with reticle WF16X/13mm WF20X/10mm P5X P10X P16X 4X/0.10 10X/0.25 40X(spring)/0.65 60X(spring)/0.85 100X(spring, oil)/1.25 4X 10X 40X(spring) 100X(spring, oil) Binocular sliding head, 360° swiveling Trinocular sliding head, 360° swiveling Binocular Gemel head, 360° swiveling Trinocular Gemel head, 360° swiveling C-mount adjustable photo port. 23mm adjustable photo port. Simultaneous Viewing Coaxial focusing system Quadruple nosepiece 130mm x 140mm mechanical stage Moving range: 50mm x 76mm Abbe, NA=1.25 Iris diaphragm Halogen light with dimmer ф50mm mirror 6V/20W Blue/yellow/green B490 T490 DIN Achromatic Objectives Plan Objectives 45° Viewing Head 30° Viewing Head Trinocular Port Focusing Nosepiece Stage Condenser Diaphragm Illumination Lamp Filter 14 490 Series Biological Microscope Con guration drawing CAMERA VIDEO WF10X18 WF10X18P WF15X16 WF20X10 EP5 EP10 EP16 CCD 400MH45 400BH45 490BH30G 400TH45 490TH30G 400PH 4. Con guration drawing 15 A100X A40X A10X A4X PA100X PA60X PA40X PA10X PA4X 400MS 400CD1.25 DRY100 OIL100 400NP4 400BL 400MR 400FB 490 SERIES 5. Optional Accessories Optional Accessories 490 SERIES There is a variety of accessories available that can enhance your microscope’s capabilities and give it additional features. Some of them are listed in the table below: NAME Eyepiece DESCRIPTION 5X MODEL # EP5X23 20X 25X 10X with pointer 10X with reticle 2X 5X EP20X23 EP25X23 EP10X23P EP10X23R A2X A5X A20X A60X PA4X PA10X PA40X PA100X DK-DRY100 DK-OIL100 MD400 (350K) MD600 (1.3MP) MD700 (2.0MP) MD800 (3.0MP) MD900 (5.0MP) MD1000 CCD-NP Objective 20X 60X Plan 4X Plan 10X Plan 40X Plan 100X Dry Dark eld Condenser Oil Dark eld Condenser CMOS Digital For obtai ning 20X, 50X, 200X and 500X magni cation powers For obtaining 80X, 200X, 800X and 2000X magni cation powers For obtaining 250X and 2500X magni cation powers For easier identifying of objects For measuring objects For obtaining 20X and 32X magni cation powers For obtaining 50X and 80X magni cation powers For obtaining 200X and 320X magni cation powers For obtaining 600X and 960X magni cation powers For obtaining high quality high resolution images For obtaining low power dark eld images For obtaining high power dark eld images For obtaining still or live digital images For obtaining high quality still or live digital images For live images on TV PURPOSES Dark eld Condenser Camera CCD Digital CCD TV Video 16 6. Parameters/Technical terms Optional Accessories cont’d... Case Phase Contrast Kit Stage Warmer Fluorescent Kit Aluminum case Hard wood case Simple Turret AC-400 WC-400 PCS PCT TCS-100 FK-YX 490 SERIES For carrying microscope around For storage Phase contrast images High quality phase contrast images For controlling stage temperature Fluorescent images Please nd more accessories on our website at: www.AmScope.com 6-1 Objectives Type Magni cation 4X 10X 40X 60X 100X 4X 10X 40X 100X Numerical Aperture (N.A.) 0.10 0.25 0.65 0.85 1.25 0.10 0.25 0.65 1.25 Medium Air Air Air Air Cedar oil Air Air Air Cedar oil Par-focal Distance (mm) 45 45 45 45 45 45 45 45 45 Magni cation Marks (color ring) Red Yellow Light blue Deep blue White Red Yellow Light blue White DIN Achromatic Objective (195mm) Plan Objective (195mm) 6-2 Eyepieces Type Magni cation Field of view (mm) Wide eld eyepiece 10X 15X 20X ф18 ф13 ф11 5X ф18 Plan eyepiece 10X 16X ф18 ф15 17 6. Parameter/technical terms 6-3 Electrical system 490 SERIES There are two options for electrical systems. Both are equipped with 6V/20W halogen lamps. (1) 220V∼240V power supply: 220V∼240V ±10%, 50Hz This electrical system is CE and GS certi ed (2) 100V∼120V power supply: 100V∼120V ±10%, 60Hz This electrical system is UL certi ed 6-4 Parameters (2) Field of view: (1) Total magni cation: 20X∼1600X ф0.8mm∼ф4.5mm (2) Mechanical tube length: (4) Object to primary image distance: (5) Fine focusing sensitivity: 160mm 195mm 0.002mm · 6-5 Technical terms (1) Total magni cation= magni cation of objective multiplied by magni cation of eyepiece (2) Field of view= line field of view of the eyepiece divided by the magnification of the objective (3) Object to primary image distance= n·sinα (max), N.A. is an important parameter that marks the features of the objective and condenser. The “n” is the refractive index of the medium (air or immersion oil) between the 18 6. Parameter/technical terms 490 SERIES objective lens and the specimen. The “α ” is 1/2 the aperture angle. The larger the N.A., the high the resolution of the objective (4) Object to primary image distance = the distance between the object plane to the primary image plane. The conjugate distance is xed (5) Mechanical tube length = the distance between the objective shoulder and the ocular shoulder 19 7. Troubleshooting 490 SERIES Troubleshooting for the 490 Series Biological Microscope SYMPTOM CAUSE REMEDY OPTICAL ISSUES One side of the eld of view is darker The nosepiece is misaligned Stain or dust has accumulated on the condenser, object, eyepieces, or base lens Turn the nosepiece until it clicks into place Clean the lens Clean the specimen Clean the lens Add the cover glass Use glass w/0.17mm thickness Flip it over Clean lens thoroughly Use immersion oil Pop the air bubbles Use standard cedarwood oil Adjust the iris diaphragm Clean the lens Adjust the condenser Secure slide to the stage with clips Turn the nosepiece until it clicks into place Adjust the diaphragm Adjust the condenser Obstructions Stains has accumulated on the are observed in specimen the eld of Stains have accumulated on the lens view There is no cover glass on the slide The cover glass is not standard The specimen is upside down The immersion oil has accumulated on the dry objective Unclear Image No immersion oil used with the 100X objective Air bubble in the immersion oil Used wrong oil The aperature is not open to an appropriate diameter Stain or dust has accumulated on the lens in the inlet of the head The condenser is not in the right position One side of the eld of view is dark or the image moves while focusing The eld of view is not bright enough The specimen slide is not xed The nosepiece is not in the right position The iris diaphragm is not big enough The condenser is not in the right position Stain or dust has accumulated on the Clean the lens condenser, objective, eyepieces, or base lens 20 7. Troubleshooting 490 SERIES Troubleshooting for the 490 Series Biological Microscope cont’d... SYMPTOM CAUSE REMEDY OPTICAL ISSUES The color of the The brightness-adjustment knob is not in the right position image is not accurate No lter is used Adjust the brightnessadjustment knob Use desired lter Flip the cover glass over Use glass w/0.17mm thickness Adjust the slide MECHANICAL ISSUES The objective touches the cover glass Unable to move the slide smoothly Focus-knob does not turn Stage declines by itself The coarsefocusing knob won’t raise the stage The nefocusing knob won’t raise the stage The bulb ickers The microscope does not light up The cover glass is upside down The cover glass is not standard The slide is not secured correctly The mechanical stage is not properly secured Tighten the mechanical stage Tension knob is too tight The tension knob is too loose Loosen it a little Tighten it a little Loosen the limit-stop knob and lock it after refocusing Limit-stop knob locked Limit-stop knob locked Loosen the limit-stop knob and lock it after refocusing ELECTRICAL ISSUES The bulb is close to burning out The microscope is unplugged The bulb is not inserted correctly The bulb burned out The fuse burned out Replace the bulb Insert it correctly Check the power cable ‘s plug Replace the bulb Replace the fuse Use the correct power supply or get a voltage adapter Use correct power supply Replace w/correct bulb The fuse burns The voltage is too high out frequently The bulb burns out frequently The voltage is too high Used wrong bulb 21