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(A Chartered University)


Instructor: Fareeha Naz, room number S-339

Office hours: Monday, Wednesday, Thursday 12:00-1:00 E-mail: Course Information: Title: Biochemistry. Course Number: Biol. 313 Number of Credit Hours: 04 Pre-requisite: Chem. 110 for non -science students Time Schedule: Monday, Wednesday and Friday 09:00-09:50 Lab. Monday 10:00-10:50 Text Books: I will recommend a number of books, each for a particular topic. Some of the text books are: 1. Eric E Conn and P. K. Stumpf. Outline of Biochemistry John Wiley & Sons. Inc. New York. 6th Ed. 2. Lehningers Principles of Biochemistry by D. I. Nelson and M. M. Cox, 4rth Ed. 3. D. Voet & J. G. Voet. Biochemistry. John Wiley Course Description: The course introduces the molecular logic of life in general. It will include a survey of structure and reactions of major Biomolecules of cell i.e., carbohydrates, Lipids, Proteins and Nucleic Acids and Enzymes Objectives of the Course To emphasize the chemical universality of living things To provide the background for further study in biological sciences and advanced biochemistry courses To develop an understanding of names, structure and function of biological molecules and carry out their extraction , analysis and estimation To gain understanding of enzymatic reactions To develop a hypothesis and design and execute experiments to test it

Learning outcomes: After the successful completion of this course, students will be able to Understand the chemical nature of molecules of life Appreciate the universality of chemical make up of living things from simplest to the most complex life form

Use laboratory equipment and techniques for qualitative and quantitative analysis and design research projects in biochemistry, physiology and cell biology and to write a report of a scientific experiment.

Course Policies: Any student who misses the examination or fails to submit the assignment must make up for the default within 7 days of the original date; otherwise he/she will not be graded for that particular exam/assignment. There is no re-sit final examination Academic dishonesty will not be tolerated. You will be dropped from the course if found cheating in the examination. Assignments and lab. Reports are expected to be your original work. Plagiarized work carries no credit Course evaluation: There will be two quizzes of 5 marks each Examinations: There will be two exams, a midterm of 20 marks of 50 minute duration and a final examination at the end of term of 40 marks and of 2 hours duration Assignment carries 10 marks. Lab. Exam of 15 marks will be based on performance and participation in lab project Attendance and class room participation carries 5 marks. Full credit will be given for 95-100 attendance Grading: Total 100 points (Exam 60, quizzes 10, assignment 10, lab, 15, attendance 5) A = 93-100, A- =90-92, B+= 87-89, B = 83-86, B- = 80-82, C+ = 77-79, C = 73-76, C- = 70-72, D+ = 67-69, = 60-66, F = 59 or Below Weekly Schedule: The course will be completed in 14 weeks, including classroom lectures, lab exercises and group discussions. First Week, February 27 Introduction to Biomolecules, Chemistry of Carbon Compounds. Carbon as the element of life Second Week, March 5 Classification of Carbohydrates. Structural formulae of simple sugars, mutarotation sugar acids, sugar alcohols Third Week, March 12 Oligosaccharides; maltose, sucrose, cellobiose Fourth Week, March 19 Polysaccharides; starch, cellulose and glycogen Quiz 1 D

Fifth Week, March 26 Lipids. Chemical natrure, classification and biologic importance of fatty acids. Sixth Week, April 2 Triglycerdides, phospholipids, glycololipids, waxes Seventh Week, April 9 Structure of amino acids, their electrochemical properties Eighth Week, April 16 Classification of proteins. Structural levels of protein Ninth Week, April 23 Structural level of protein continued Tenth Week, April 30 Nucleic acids, purine and pyramidine bases, nucleosides and nucleotides Eleventh Week, May 7 Structure and properties of DNA and RNA Twelfth Week, May 14 Enzyme; nature, general characteristics and classification Thirteenth Week, May 21 Factors affecting enzyme activity, enzyme inhibition, allosteric enzymes Fourteenth Week, May 28 Kinetics of enzyme activity, mode of action, isozymes June 11 End of the Term Examination Quiz 2 Mid-term examination