Dallas County Southwestern Institute of Forensic Sciences

TOXICOLOGY LABORATORY TRAINING MANUAL Version 2.0

Authorized by:

Elizabeth L. Todd, Ph.D., Chief Forensic Chemistry Chris Heartsill, Supervisor Toxicology Laboratory Chris Heartsill, Acting Quality Manager January 22, 2008

Effective date:

This is a non-controlled copy of a controlled document

Dallas County Institute of Forensic Sciences Toxicology Training Manual Summary of Changes from Previous Manual Version Previous Version: Toxicology Training Manual, version 1.0 Current Version: Toxicology Training Manual, version 2.x 1. Update training form consistent with practice that completion of training is acknowledged by the trainee, the supervisor and the section chief. 2. Sections were added to document running initial assays under the direction of an experienced analyst. 3. Correction of various typographical and grammatical errors. 4. Various non-substantive changes in wording and format to improve clarity. 5. Conversion of the hardcopy manual to an electronic form.

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Summary of Changes Version 2.0

This is a non-controlled copy of a controlled document

Toxicology Training Manual Version 2.x Revisions and Corrections Effective Date Description Authorized by

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Corrections and Revisions Version 2.0

This is a non-controlled copy of a controlled document

TOXICOLOGY LABORATORY TRAINING MANUAL - OVERVIEW The Institutes employee training program is established in Training Programs found in the Quality Manual. All new employees and newly assigned employees must complete an applicable training program which is documented using training checklists. The training checklist should be completed by the trainee and trainer throughout the training process with input and guidance of the Supervisor and/or Section Chief. The checklist and associated instrument documents will be submitted to the Quality Manager. I. Toxicology Chemist II

The goal of the Toxicology Chemist II training program is as follows: Establish proficiency in all types of analyses and instrumental techniques performed by the Laboratory Develop an understanding of the analytical techniques used Develop proficiency in the basic operation, troubleshooting and repair of instrumentation Select appropriate specimen for analysis Appropriately apply analytical procedures for the accurate analysis of specimen routinely submitted to the Laboratory Produce a properly documented case file Effectively evaluate instrumental output and results of standard and quality control samples Effectively communicate analytical procedures and results of analysis Participate in technical case review Seek supervisory assistance in an appropriate manner

The Toxicology Procedure Manual including training notes and selected reference material form the basis of the training program. The analyst is referred to the Section Administrative Manual for information regarding the Sections procedure for receiving off-site training. The training process for the Toxicology Chemist II is divided into three stages: Stage 1 - Basic Training Stage 1 training includes developing proficiency in a core set of analysis that all Toxicology Chemists are expected to perform and completing required technical training regarding instrumentation, specimen preparation, and postmortem toxicology. The core set of analyses learned in this stage of training includes the alkaline and acid/neutral drug screens, alcohols and acetone, carboxyhemoglobin, and electrolytes assays. Proficiency with this core set of analyses must be demonstrated prior to completion of the probationary period (6 months) and is usually completed within two to three months depending upon the experience of the employee. In addition to these analyses, each toxicologist must complete required technical training encompassing instrument theory and operation, instrument maintenance, basic principles in postmortem toxicology, and specimen selection and preparation. At the conclusion of Stage 1
Dallas County Institute of Forensic Sciences Toxicology Training Manual 1 Training Overview Version 2.0

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training, each employee demonstrates competency through a written exam and/or moot court. Refer to the Stage 1 Checklist for details of this portion of the training program. Stage 2 In Stage 2 training, the Toxicology Chemist II is trained in screening procedures including the ELISA (enzyme linked immuno-sorbent assay) and the FPIA (fluorescence polarization immunoassay). They also receive training in more advanced GC/MS data analysis and data acquisition for both selected ion monitoring and full scan modes. Completion of Stage 2 should be completed within nine months of the employees start date and will begin immediately after completion of Stage 1. Upon successful completion of Stage 2, the Toxicology Chemist II will be proficient in performing and evaluating most types of analyses performed by the Laboratory and will begin participating as a technical/peer reviewer for the techniques in which they have demonstrated competency. Refer to the Stage 2 Checklist for details of this portion of the training program. Stage 3 Stage 3 encompasses the ongoing training that occurs throughout the career of the Toxicology Chemist II. As applicable, the analyst is trained in other remaining assays offered by the Toxicology Laboratory. As the level of experience and training continues to expand, the employee will expand participation in technical/peer review, case management, instrument troubleshooting and repair, and methods development. The Toxicology Chemist II should have responsibility for at least one special assay within one year of the start date of employment. Refer to the Stage 3 Checklist for details of this portion of the training program.

II.

Toxicology Chemist III

The goal of the Toxicology Chemist III training program is as follows: Demonstrate competence at the Toxicology Chemist II level; refer to the Toxicology Chemist II Checklist Develop proficiency in interpretation of toxicology results including effects on performance and toxicity Develop proficiency in applicable principles of pharmacology including extrapolation of alcohol results Effectively communicate analytical procedures, results of analysis, and interpretation of results Develop expertise as expert witness Develop skills in advanced instrument troubleshooting and repair

The Toxicology Chemist III position must successfully demonstrate competence beyond the Toxicology Chemist II level prior to the completion of the probationary period. The initial training for the Toxicology Chemist III includes required technical training regarding the effects of alcohol and drugs and how they relate to toxicity, performance, and driving. The Toxicology
Dallas County Institute of Forensic Sciences Toxicology Training Manual 2 Training Overview Version 2.0

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Chemist III also receives training in basic pharmacology and its application in extrapolating alcohol results. Once trained, the Toxicology Chemist III will have primary responsibility for the legal alcohol/drug screen program in the Toxicology Laboratory including analytical work, interpretation of analytical results, and applicable testimony in a court of law. The Toxicology Chemist III serves as an assistant supervisor providing leadership and oversight of Laboratory operation as directed by the Supervisor and/or Section Chief. Training should be completed within one year of hire; competency is evaluated using a written examination and moot court. Refer to the Toxicology Chemist III Checklist for details of this training program. III. Toxicology Supervisor

The Toxicology Supervisor must demonstrate proficiency at the Toxicology Chemist III level. In addition, the supervisor will receive applicable training in policies and procedures of Dallas County and other management skills training. Training documentation is retained by the Quality Manager.

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Training Overview Version 2.0

TRAINING CHECKLIST Administrative Procedures

Trainee: ____________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___ Attend County Orientation ___Read IFS Quality Manual ___ Read IFS Security Manual ___ Read Section Administrative Manual ___ Read IFS Code of Ethics and Conduct ___ Read Toxicology Procedure Manual Sections 1-19, complete Evidence Management Checklist ___ Enter time and attendance for one week ___ Review operation of the drug safe ___ Review peer review and proficiency process _____ _____ _____ _____ _____ _____ Instructor _____ _____ _____ _____ _____ _____ Date ______ ______ ______ ______ ______ ______

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___ Review QC processes: balance, pipettors, etc. _____

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Administrative Training Checklist Version 2.0

TRAINING CHECKLIST Safety

Trainee: ___________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___ Read IFS Environmental Health and Safety Manual ___ Attend Safety Training ___ Receive Personal Protective Equipment Lab coat Safety Glasses ___ Safety Equipment Locations Gloves Eyewash Stations Emergency Shower First Aid Kits Fire Blankets Emergency Phone Numbers Fire Extinguishers Evacuation Rules and Routes Meet Floor Emergency Wardens _____ Instructor _____ Date ______

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When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Administrative Training Checklist Version 2.0

TOXICOLOGY TRAINING CHECKLIST TOXICOLOGY CHEMIST II / LITERATURE AND TECHNICAL REVIEW Trainee:__________________________________________________(Print) Instructor:________________________________________________(Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee ____Read Chapter 1, Postmortem Forensic ______ Toxicology, in Principles of Forensic Toxicology ____Read Chapter 5, Specimen Preparation, in Principles of Forensic Toxicology ______ Initials Instructor ______

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____Read Chapter 13 Part 3, Gas Chromatography______ ______ _____ in Forensic Toxicology, pp 718 736 in Modern Practice of Gas Chromatography ____Read Chapter 7, Chromatography, in Principles of Forensic Toxicology ______ ______ _____

____Read Chapter 2 Sections 2.2 and 2.3, ______ ______ _____ General Aspects of Gas Chromatography and Gas Chromatography, in Modern Practice Of Gas Chromatography ____View Capillary Installation, Restek ______ ______ ______ _____ _____

____View Maintaining Your Agilent GC System ______ Agilent Technologies ____View Maintaining Your GC/MS System, Agilent Technologies ____View GC Inlet Resource Guide, Agilent Technologies ______

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____Read Chapter 2, pp 38-68, Agilent ______ ______ Technologies Hardware Manual 5973N Mass Selective Detector ____Read Chapter 9, Mass Spectrometry, in Principles of Forensic Toxicology ______ ______

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Dallas County Institute of Forensic Sciences Toxicology Training Manual

Toxicology Chemist II/Literature Review Version 2.0

____Read Chapter 1, pp 1-14 in Interpretation of Mass Spectra

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______ ______ _____ ____Become familiar with Disposition of Toxic Drugs and Chemicals in Man, PDR, The Merck Index, and Clarks Isolation and Identification of Drugs ____Read GC Instrument Procedure and Maintenance ____Read GC/MS Instrument Procedure and Maintenance ___GC/MS Operation: ___ Auto Tune ___ Target Tune ___ Sequence Table ___ Generating a Spectrum ___ Adding Spectra ___ Subtracting Spectra ___ Generating a Library Search ___ Generating an Ion Chromatograph ___ Air and Water Check (leaks?) ___ Criteria for Acceptable Spectra ___ Identification of Unknown Spectra ___GC Operation ___ Maintenance (insert, seal, septum) ___ Routine operation including: calibration standard computer operation reprocessing data ____Observe and discuss court testimony ______ ______ _____

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____Successfully complete written exam ______ ______ _____ When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief:
Dallas County Institute of Forensic Sciences Toxicology Training Manual 2

Date: Date: Date:

Toxicology Chemist II/Literature Review Version 2.0

TOXICOLOGY TRAINING CHECKLIST TOXICOLOGY CHEMIST III Trainee:__________________________________________________(Print) Instructor:________________________________________________(Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee Instructor Date ____Read Principles of Forensic Toxicology ____Read Medicolegal Aspects of Alcohol ____Read Forensic Science Review ______ ______ ______ ______ ______ ______ _____ _____ _____

______ ______ _____ ____Become familiar with Casarett and Doulls Toxicology, Goldfranks Toxicologic Emergencies, and Ellenhorns Medical Toxicology ____Observe and discuss court testimony 6 times ______ ______ ______ ______ ______ ______ ______ ______ ______ ______ ______ ______ ______ _____ _____ _____ _____ _____ _____ _____

____Complete training in basic pharmacology ______ and extrapolation with written examination ____Attend training on drug/alcohol impairment and toxicity ____Successfully complete moot court ____Successfully complete comprehensive written exam ______

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____Court Monitoring Evaluation for initial ______ ______ _____ court testimony When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Date: Supervisor: Section Chief: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Toxicology Chemist III Version 2.0

Toxicology Chemist Training Summary

Toxicologist____________________________________
Stage I Alkaline Drug Screen Acid Neutral Drug Screen Alcohols and Acetone Electrolytes Carbon Monoxide Literature Review Complete Written Exam Date Stage II ELISA ADX SIM/Scan Training SIM Method: Peer Review Participation Date Stage III SIM analysis (Special Assay) AN Quant THC Blood THC Urine Opiates Cocaine and Metab. Ethylene Glycol Ethchlorvynol Ethosuximide Valproic Acid GHB Metals Lead Lithium Arsenic Mercury Volatiles GC/MS Identification Multiple Headspace Static Headspace Misc. Acetaminophen Salicylic Acid Cyanide Date

Supervisor: Section Chief:

Toxicology Chemist III Literature Review Observe Court Testimony Basic Pharmacology and Extrapolation Impairment and Toxicity Training Moot Court Complete Written Exam

Date

TOXICOLOGY TRAINING CHECKLIST SAMPLE LOG-IN, SAMPLE TRACKING, AND EVIDENCE RECEIPT/RELEASE Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Toxicology Procedure Manual regarding evidence management _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Review of Chain of Custody and Proper Seal ___ Review of Authorized Personnel ___ Receipt of Medical Examiner cases ___ Location of paperwork/case folders ___ production of case folder ___ evidence receipt paperwork ___ evidence release paperwork ___ administrative billing summary ___ quality control paperwork ___ Receipt of Other cases (H, D, etc.) ___ Tox LIMS ___ Walk-in Refrigerator assignments ___ incoming samples ___ samples being worked ___ samples waiting for long-term storage ___ samples in freezer ___ Filling out the workload logbooks
Dallas County Institute of Forensic Sciences Toxicology Training Manual 1

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Evidence Handling Version 2.0

___ Filing paperwork in Case Folder

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___ Supervised Sample Log-in ___ 10 medical examiner cases ___ 10 outside agency cases ___ Review of referral lab process ___ Review of special paperwork/requests ___ infectious disease testing ___ metabolic testing ___ histology

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When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Evidence Handling Version 2.0

TOXICOLOGY TRAINING CHECKLIST ACETAMINOPHEN QUANTITATION BY GC Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Acetaminophen Quantitation and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of GC ___ Reporting Results and Coding Sheets ___ Successful Duplicate + QC Run 10 quality control samples 10 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

When all the areas have been checked off and initialed, the trainee, supervisor, and section Chief will sign and date this form. The form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: __________________________________ Supervisor: Section Chief: Date: _____________ Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Acetaminophen by ADX Version 2.0

TOXICOLOGY TRAINING CHECKLIST ACID-NEUTRAL DRUG SCREEN Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Acid-Neutral Drug Screen and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ GC/MS ChemStation Operation ___ Create a Sequence Table ___ Save and Run Sequence ___ GC/MS Operation ___ Reporting Results and Coding Sheets ___ Successful Duplicate + QC Run 10 quality control samples 15 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ ______ ______ ______

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Dallas County Institute of Forensic Sciences Toxicology Training Manual

Acid/Neutral Screen Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Acid/Neutral Screen Version 2.0

TOXICOLOGY TRAINING CHECKLIST ACID-NEUTRAL DRUG QUANTITATION Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee ___Re-read Acid-Neutral Drug Screen and associated training literature ___Read Selective Ion Monitoring _____ Initials Instructor _____ Date _____

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Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List _____ _____ ______ ___ Preparation of Standards ___ Preparation of Calibrators ___ Preparation of Controls ___ Preparation of Reagents ___ GC / MS Operation ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Successful Quality Control Run 10 quality control samples 10 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

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Dallas County Institute of Forensic Sciences Toxicology Training Manual

Acid/Neutral Quantitation Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Acid/Neutral Quantitation Version 2.0

TOXICOLOGY TRAINING CHECKLIST ALCOHOLS AND ACETONE Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Alcohols and Acetone by Headspace Gas Chromatography and training literature ___ Read Operating Instructions for Headspace Instruments (Instruments and Computer) Instructor Date

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Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Headspace Autosampler Operation ___ Shimadzu GC Solutions Operation ___ ___ ___ ___ ___ ___ ___ ___ Create a Sample List Create a Recalc List Save Sample List Run a Sample List Reprint Report Load a Chromatogram Where to Edit the Methods Where to Find Custom Reports _____ _____ _____ _____ ______ ______ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ _____ _____

___Interpretation of Chromatograms ___ Reporting Results and Coding Sheets

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Alcohols and Acetone Version 2.0

___ Successful Quality Control Run ___ Successful Duplicate Run 20 quality control samples 20 repeat samples where available 3 supervised runs

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When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Alcohols and Acetone Version 2.0

TOXICOLOGY TRAINING CHECKLIST ALKALINE DRUG SCREEN

Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Alkaline Drug Screen ___ Read ChemStation Software Notes _____ _____ Instructor _____ _____ Date ______ ______

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generating Sample Work List ___ Quality Control ___ Preparing Samples for Analysis ___ Gas Chromatography Operation ___ ChemStation Operation ___ Create a Sequence Table ___ Save and Run Sequence _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Alkaline Screen Version 2.0

Initials Trainee Instructor Date

___ Reporting Results and Coding Sheets ___ Successful Duplicate + Q.C. Run 12 quality control samples 11 repeat samples where available 3 supervised runs

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All positive results require confirmation using Gas Chromatography/ Mass Spectrometry (GC/MS). After completing required reading, listed below, the trainee will spend approximately 1 week with instructor covering all aspects of GC/MS analysis including the following areas. ___ Read Gas Chromatography/ Mass Spectrometry _____ ___ 5973 ChemStation Operation ___ Create a Sequence Table ___ Save and Run Sequence ___ Run Alkaline Extractions on GC/MS ___ Compare Total Ion Chromatogram to GC Run ___ Run and Interpret Library Searches _____ _____ _____ ______ ______

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When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Alkaline Screen Version 2.0

TOXICOLOGY TRAINING CHECKLIST ARSENIC BY AA Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Arsenic by AA and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of Atomic Absorption ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples 10 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief:
Dallas County Institute of Forensic Sciences Toxicology Training Manual 1

Date: Date: Date:

Arsenic by AA Version 2.0

TRAINING CHECKLIST TOXICOLOGY / BENZODIAZEPINES BY GC/MS Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee Instructor Date ___Read Selective Ion Monitoring _____ _____ ______ ___Read Benzodiazepines by GC/MS and training literature _____ _____ ______ ___Read Handbook of Derivatives for Chromatography _____ _____ ______ Section 4.4.1 page 57-58 ___Read Gas Chromatography Derivatization in the _____ _____ ______ Pierce Catalog and Handbook, page T-231-238 Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List _____ _____ ______ ___ Preparation of Standards ___ Preparation of Calibrators ___ Preparation of Controls ___ Preparation of Reagents ___ Derivatization Procedure ___ GC / MS Operation ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Successful Quality Control Run 10 quality control samples 15 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ _____ ______

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Dallas County Institute of Forensic Sciences Toxicology Training Manual

Benzodiazepines by GC/MS Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section Chief will sign and date this form. The form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: __________________________________ Supervisor: Section Chief: Date: _____________ Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Benzodiazepines by GC/MS Version 2.0

TOXICOLOGY TRAINING CHECKLIST CARBOXYHEMOGLOBIN Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Carboxyhemoglobin and training literature Instructor Date ______

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Trainee will spend approximately 3 days with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Nova Biomedical Critical Care XPress Operation ___ Daily Controls ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Reporting Results and Code Sheets ___ Successful Quality Control Run 10 quality control samples 15 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Carboxyhemoglobin Version, 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Carboxyhemoglobin Version, 2.0

TOXICOLOGY TRAINING CHECKLIST COCAINE AND METABOLITES BY GC/MS Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee ___Read Selective Ion Monitoring ___Read Cocaine and Metabolites by GC/MS and training literature ___Read Gas Chromatography Derivatization in Pierce Catalog and Handbook, page T-244 _____ Initials Instructor _____ Date ______ ______

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Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Standards ___ Preparation of Calibrators ___ Preparation of Controls ___ Preparation of Reagents ___ Derivatization Procedure ___ GC / MS Operation ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Successful Quality Control Run 10 quality control samples 15 repeat samples where available 3 supervised runs
Dallas County Institute of Forensic Sciences Toxicology Training Manual 1 Cocaine by GC/MS Version 2.0

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When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Cocaine by GC/MS Version 2.0

TOXICOLOGY TRAINING CHECKLIST CYANIDE QUANTITATION Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Cyanide Quantitation and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of pH/Ion Specific Electrode Meter ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Cyanide Version 2.0

TOXICOLOGY TRAINING CHECKLIST ELECTROLYTES Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Electrolytes By NOVA Biomedical Critical Care XPress _____ Instructor _____ Date ______

Trainee will spend approximately 3 days with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ NOVA Biomedical CCX Operation / Maintenance ___ Quality Control ___ Preparation of Samples for Analysis ___ Generation of Sample Work List ___ Calculation Rules ___ Successful Quality Control Run 10 quality control samples 15 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Electrolytes Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Electrolytes Version 2.0

TOXICOLOGY TRAINING CHECKLIST DRUG SCREENING BY ELISA Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read ELISA and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of Tecan Miniprep and Reader ___ Reporting Results and Coding Sheets ___ Successful Duplicate + QC Run 3 repeat full runs where available 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

ELISA Version 2.0

TOXICOLOGY TRAINING CHECKLIST ETHYLENE GLYCOL Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Ethylene Glycol Quantitation and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of GC/MS ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ ______ ______ ______

_____ _____

_____ _____

______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Ethylene Glycol Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Ethylene Glycol Version 2.0

TOXICOLOGY TRAINING CHECKLIST GAMMA HYDROXYBUTYRATE Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Gamma Hydroxybutyrate and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of GC/MS ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Reporting Results and Coding Sheets ___ Successful Duplicate + QC Run 10 quality control samples 15 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ ______ ______ ______

_____ _____

_____ _____

______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manaual

GHB Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manaual

GHB Version 2.0

TOXICOLOGY TRAINING CHECKLIST LEAD BY GRAPHITE FURNACE AA Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Lead by Graphite Furnace AA and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of Atomic Absorption ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Lead by AA Version 2.0

TOXICOLOGY TRAINING CHECKLIST LITHIUM BY AA Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Lithium by AA and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of Atomic Absorption ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Lithium by AA Version 2.0

TOXICOLOGY TRAINING CHECKLIST MERCURY BY AA Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Mercury by AA and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of Atomic Absorption ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Mercury by AA Version 2.0

TOXICOLOGY TRAINING CHECKLIST METHOCARBAMOL by GCMS QUANTITATION Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee ___Re-read Methocarbamol and associated training literature ___Read Selective Ion Monitoring _____ Initials Instructor _____ Date _____

_____

_____

_____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List _____ _____ ______ ___ Preparation of Standards ___ Preparation of Calibrators ___ Preparation of Controls ___ Preparation of Reagents ___ GC / MS Operation ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Successful Quality Control Run 10 quality control samples 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

_____

_____

______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Methocarbamol Quantitation Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Methocarbamol Quantitation Version 2.0

TOXICOLOGY TRAINING CHECKLIST OPIATES BY GC/MS Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee Initials Instructor

Date

___Read Selective Ion Monitoring _____ _____ ______ ___Read Opiate by GC/MS and training literature _____ _____ ______ ___Read Handbook of Derivatives for Chromatography _____ _____ ______ Section 4.4.1 page 57-58 ___Read Gas Chromatography Derivatization in the _____ _____ ______ Pierce Catalog and Handbook, page T-231-238 Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List _____ _____ ______ ___ Preparation of Standards ___ Preparation of Calibrators ___ Preparation of Controls ___ Preparation of Reagents ___ Derivatization Procedure ___ GC / MS Operation ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Successful Quality Control Run 10 quality control samples 15 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ _____ ______

_____

_____

______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Opiates by GC/MS Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Opiates by GC/MS Version 2.0

TOXICOLOGY TRAINING CHECKLIST SALICYLATES Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee ___Read Salicylates and associated training literature _____ Initials Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of Fluorescence Spectrophotometer ___ Reporting Results and Coding Sheets ___ Successful Duplicate + QC Run 10 quality control samples 10 repeat samples where available 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Salicylates Version 2.0

Dallas County Southwestern Institute of Forensic Sciences

TOXICOLOGY LABORATORY TRAINING MANUAL Version 2.0

Authorized by:

Elizabeth L. Todd, Ph.D., Chief Forensic Chemistry Chris Heartsill, Supervisor Toxicology Laboratory Chris Heartsill, Acting Quality Manager January 22, 2008

Effective date:

TOXICOLOGY TRAINING CHECKLIST CANNABINOIDS IN BLOOD BY GC/MS Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee Initials Instructor

Date

___Read Selective Ion Monitoring _____ _____ ______ ___Read Cannabinoids in Blood by GC/MS _____ _____ ______ ___Read Handbook of Derivatives for Chromatography _____ _____ ______ Section 4.4.1, pages 57-58 ___Read Gas Chromatography Derivatization in the _____ _____ ______ Pierce Catalog and Handbook, page T-231-238 Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List _____ _____ ______ ___ Preparation of Standards ___ Preparation of Calibrators ___ Preparation of Controls ___ Preparation of Reagents ___ Derivatization Procedure ___ GC / MS Operation ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Successful Quality Control Run 10 quality control samples 15 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ _____ ______

_____

_____

______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Cannabinoids in Blood Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Cannabinoids in Blood Version 2.0

TOXICOLOGY TRAINING CHECKLIST CARBOXY-THC IN URINE BY GC/MS Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee Initials Instructor

Date

___Read Selective Ion Monitoring _____ _____ ______ ___Read Carboxy -THC in Urine by GC/MS _____ _____ ______ ___Read Handbook of Derivatives for Chromatography _____ _____ ______ Section 4.4.1, pages 57-58 ___Read Gas Chromatography Derivatization in the _____ _____ ______ Pierce Catalog and Handbook, page T-231-238 Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List _____ _____ ______ ___ Preparation of Standards ___ Preparation of Calibrators ___ Preparation of Controls ___ Preparation of Reagents ___ Derivatization Procedure ___ GC / MS Operation ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Successful Quality Control Run 10 quality control samples 15 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ _____ ______

_____

_____

______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Carboxy-THC in Urine Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Carboxy-THC in Urine Version 2.0

TOXICOLOGY TRAINING CHECKLIST VALPROIC ACID, ETHOSUXIMIDE, ETHCHLORVYNOL Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Valproic Acid, Ethosuximide, Ethchlorvynol _____ and associated training literature Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of GC/MS ___ Instrument Parameters ___ SIM Parameters ___ Data Analysis ___ Reporting Results and Coding Sheets ___ Successful QC Run (only one analyte required) 10 quality control samples 5 repeat samples where available 3 supervised runs _____ _____ _____ _____ _____ _____ ______ ______ ______

_____ _____

_____ _____

______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Valproic Acid, Ethosuximide, Ethchlorvynol Version 2.0

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date:

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Valproic Acid, Ethosuximide, Ethchlorvynol Version 2.0

TOXICOLOGY TRAINING CHECKLIST VOLATILES BY GC/MS Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Volatiles by GC/MS and associated training literature Instructor _____ Date _____

_____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of GC/MS ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Manual

Volatiles by GC/MS Version 2.0

TOXICOLOGY TRAINING CHECKLIST VOLATILES BY HEADSPACE GC Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Volatiles by Headspace GC and associated training literature _____ Instructor _____ Date _____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of Headspace GC ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Checklist

Volatiles by Headspace GC Version 2.0

TOXICOLOGY TRAINING CHECKLIST VOLATILES BY MULTIPLE HEADSPACE Trainee: ____________________________________________ (Print) Instructor: __________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___Read Volatiles by Multiple Headspace and associated training literature Instructor _____ Date _____

_____

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. ___ Generation of Sample Work List ___ Preparation of Samples for Analysis ___ Operation of Headspace GC ___ Reporting Results and Coding Sheets ___ Successful QC Run 10 quality control samples 3 supervised runs When all the areas have been checked off and initialed, the trainee, supervisor, and section chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: Supervisor: Section Chief: Date: Date: Date: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______

Dallas County Institute of Forensic Sciences Toxicology Training Checklist

Volatiles Multiple Headspace Version 2.0