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Activity 4.5 (Experiment) 42 Studying the effects of substrate concentration on salivary amylase activity Aim / To study the effects of substrate concentration on Objective of salivary amylase activity the Study Problem What are the effects of substrate concentration on Statement salivary amylase activity? Hypothesis The rate of enzymatic reaction increases with the increase in substrate concentration until it reaches a maximum rate Variables Manipulated variable: Substrate concentration / Concentration of starch suspension Responding variable: Rate of reaction / Time taken for the hydrolysis of starch to be completed Fixed variable: Enzyme concentration, pH of medium, volume of starch, volume of saliva suspension and surrounding temperature Material 0.1% starch suspension 0.2% starch suspension 0.3% starch suspension 0.4% starch suspension 0.5% starch suspension 0.6 % starch suspension 0.1% amylase solution / saliva suspension Iodine solution Apparatus Syringes White tiles with grooves Test tubes Glass rod Dropper Measuring cylinder Stopwatch Beaker Thermometer Water bath (Bunsen burner, tripod stand and wire gauze) Technique Test for the presence of starch using iodine test used Record the time taken for the hydrolysis of starch to be completed with a stopwatch

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Activity 4.6 (Experiment) 43 Studying the effect of enzyme concentration on the activity of salivary amylase Aim / To study the effect of enzyme concentration on the Objective of activity of salivary amylase the Study Problem What are the effects of enzyme concentration on Statement the activity of salivary amylase? Hypothesis The rate of enzymatic reaction increases with the increase in enzyme concentration as long as there are no other factors limiting the rate of reaction. Variables Manipulated variable: Enzyme concentration Responding variable: Rate of reaction Fixed: Substrate concentration and temperature Material 1% starch suspension 0.5% amylase solution / saliva suspension Iodine solution Distilled water Apparatus 5 ml of syringe 1 ml of syringe White tiles with grooves Test tubes Glass rod Dropper Measuring cylinder Stopwatch Beaker Thermometer Water bath (Bunsen burner, tripod stand and wire gauze) Technique Test for the presence of starch using iodine test used Record the time taken for the hydrolysis of starch to be completed with a stopwatch