Jamonline / 2(2); 2012 / 149–154 Research Article

EVN Raju et al

Journal of Atoms and Molecules
An International Online Journal
ISSN – 2277 – 1247

PHYTO CHEMICAL SCREENING AND ISOLATION OF COUMARIN COMPOUNDS FROM MEDICINAL PLANT VACHA BARK Raju EVN*1, Chinnari Harika2, Sandhya Neelima Koltikala3, Sirisha M1, Sandeep Kumar K1, Veera Reddy G1, Kavitha Madhuri M1, Sai Phani KASVR4
1 2

MITS College of Pharmacy, Kodad, Nalgonda District, Andhra Pradesh, India.

Department of Food and Nutritional Sciences, Achrya Nagarjuna University, Guntur, A.P. 3 RV Labs, Guntur, Andhra Pradesh. 4 DNR College of Engineering and Technology, Bhimavaram, West Godavari District, A.P Revised on: 20-03-2012 Accepted on: 29–03–2012

Received on: 02-03-2012 Abstract:

Vacha plant is medicinally useful plant. In order our phytochemical screening we identified a coumarins in Vacha plant. We are extracted and isolated coumarin and identified a compound in the Vacha bark. Key Words: Vacha Plant, Bark, Coumarin, IR and NMR Spectra. Introduction: VACHA is one of the most renowned herbs used for mental disorders and diseases of the nervous system. In Sanskrit Vacha literally * Corresponding author Raju EVN, Email: evnrazbiotech@gmail.com means speaking. It is a strongly aromatic, semi-aquatic perennial herb with a ginger-like stem which spreads into the ground. This rhizome part of the plant is of medicinal use. Vacha is one of the rare medicines which find mention in the Vedas. Later day acharyas worked to find more of its benefits not only to the brain and the nervous system but also to other parts of the body. It has been described as bitter and pungent in taste and hot, sharp, dry and light in effect. Dry rhizomes of vacha All rights reserved© 2011 www.jamonline.in 149

Jamonline / 2(2); 2012 / 149–154 contain yellow aromatic oil which is volatile. It also has a bitter substance known as acorin. In the ayurvedic system Vacha is used as a tonic and an anti-stammering drug.

EVN Raju et al treatment of blemishes and pimples. In many Indian homes, customarily, Vacha is

administered with honey in a minute quantity to infants on the 11th and 21st days of birth. It is believed that this practice helps the child to be mentally active and vocal. Classic ayurvedic formulations like

Experimental studies have shown that it is a potent psycho-pharmacological agent having a positive effect on the memory and the learning process. Many ancient texts have described Vacha as an anti epileptic and antihysteric herb. It is also known to possess carminative, digestive, diuretic and mildly sedative properties. Though as a psychotropic medicine Vacha is beneficial in cases of anxiety and depression, it is best used as a nervine tonic. Ayurvedic texts suggest that it is the premium herb to be used in cases of mental retardation, stupor, syncope and epilepsy. It is helpful in many other problems like anorexia, chronic gas trouble, and hypertension, sluggishness of the liver— and also in skin diseases. As a household remedy some of the common uses of Vacha are as under: Combine in equal amounts the powders of Vacha, shankhpushpi and brahmi. Half a teaspoonful of this powder mixed with one teaspoonful of honey, if taken daily, is a good adjunct in the cases of epilepsy and mental retardation. Taking with warm water, half a pinch of the vacha powder works well in the loss of appetite, flatulence, distaste, dull abdominal pain and worms. It is a herb of choice to be used in the case of loud eructations. The powder of Vacha and white sandal makes a very effective face-pack in the

sarswatarishta and sarswata churna contain vacha as the chief ingredient and are used for the promotion of memory and also in the treatment of many psychiatric problems. As a single drug, the dose of Vacha powder is 125 mg to 500 mg. Its overdose can induce vomiting and such a situation can be managed by giving the powder of saunf with lime water. Different varieties of Vacha are available in the market, but the best of them is known as ghorha bach. Materials and Methods The plant Vacha is collected from Ayurvedha hospital in kerala. The plant bark was dried under sun shade and powdered, extracted in different solvents by soxlate extraction

procedure. Initially we used Non polar solvent for removing of unsaturated fat, starch and extra non-useful chemical constitutions. Later we kept soxalate extraction in polar solvent. Methanol Coumarins evaporated are with roto with vapor. standard

isolated

protocol2. Compound is in pale yellow color. The obtained extraction is recrystalised in Methanol. The compound was sent to IICT Hyderabad for spectral data.

All rights reserved© 2011

www.jamonline.in 150

Jamonline / 2(2); 2012 / 149–154 Results and Discussions Spectral data: IR Spectra:

EVN Raju et al

Figure 1 PNMR Spectra:

Figure 2

All rights reserved© 2011

www.jamonline.in 151

Jamonline / 2(2); 2012 / 149–154

EVN Raju et al

Node CH CH OH CH3 NH CH CH CH CH CH CH3 CH3 H H

Shift 6.98 6.44 5.0 1.71 8.0 5.05 7.01 6.72 6.72 7.01 1.58 3.73 5.90 8.37

Base+Inc. 7.26 7.26 5.0 0.86 8.0 1.5 7.26 7.26 7.26 7.26 0.86 0.86 5.25 5.25

Comment 1-benzene 1-benzene Aromatic C-OH Methyl Sec. amide Methine 1-benzene 1-benzene 1-benzene 1-benzene Methyl Methyl 1-ethylene 1-ethylene

Table 1 Spectral data of PNMR CNMR Spectrum:

Figure 3 All rights reserved© 2011 www.jamonline.in 152

Jamonline / 2(2); 2012 / 149–154

EVN Raju et al

Node C CH C C CH CH C C C

Shift 160.9 112.5 152.8 113.6 127.4 112.5 157.9 118.2 146.9

Base+Inc. 166 123.3 123.3 128.5 128.5 128.5 128.5 128.5 128.5

Comment 1-carboxyl 1-ethylene 1-ethylene 1-benzene 1-benzene 1-benzene 1-benzene 1-benzene 1-benzene

Table 2 Spectral data of CNMR

Mass Spectra:

From above spectral data the final structure was constructed.

Figure 5 Structure of Coumarin Figure 4

All rights reserved© 2011

www.jamonline.in 153

Jamonline / 2(2); 2012 / 149–154 Conclusion The plant VACHA has coumarin compounds in bark. So this plant more applicable in disease treatment. References 1. www.tribuneindia.com/2001/20010926/he alth. 2. Isolation of ipomeamarone from and sweet

EVN Raju et al 11. Liu H., "Extraction and Isolation of compounds from Herbal medicines" in 'Traditional Herbal Medicine Research Methods', ed by Willow JH Liu 2011 John Wiley and Sons, Inc 12. Farinola, Nicholas; Piller, Neil (June 1, 2005). "Pharmacogenomics: Its Role in Re-establishing Coumarin as Treatment for Lymphedema". Lymphatic Research and Biology3 (2): 81–86. 13. "Frequently Asked Questions about coumarin in cinnamon and other foods" .The German Federal Institute for Risk Assessment. 14. NPR: German Christmas Cookies Pose Health Danger 15. High daily intakes of cinnamon: Health risk cannot be ruled out. BfR Health Assessment No. 044/2006, 18 August 2006

two coumarin derivatives

potato roots injured by the weevil, Cylas formicarius elegantulus, Archives of

Biochemistry and Biophysics,Volume 88, Issue 1, May 1960, Pages 150–156 3. A., King, H. K., 1970. The biosynthesis

of 4-hydroxycoumarin and dicoumarol by Aspergillus fumigatus Fresenius. Biochemical Journal 117, 237-245. 4. Treatment of Lymphedema of the Arms and Legs with 5,6-Benzo-[alpha]-pyrone. John R. Casley-Smith, Robert Gwyn Morgan, and Neil B. Piller Volume 329:1158-1163 October 14, 1993 Number 16 5. Review of benzypyrone drugs and edema 6. F. P. Schäfer (Ed.), Dye Lasers, 3rd Ed. (Springer-Verlag, Berlin, 1990). 7. F. J. Duarte and L. W. Hillman (Eds.), Dye Laser Principles (Academic, New York, 1990). 8. F. J. Duarte, Tunable Laser Optics (Elsevier-Academic, New York, 2003) Appendix of Laser Dyes. 9. U.S. Pat. No. 4175982 to Loutfy et al, issued Nov 27 1978 to Xerox Corp. 10. "The discovery of dicumarol and its sequels". Circulation 19(1): 97–107. All rights reserved© 2011

www.jamonline.in 154