41 views

Uploaded by Muhammad Rifqi Rofiuddin

- epc621_2
- troy ccr2017
- Calculation Cstr 40l
- Shimadzu_CuvetteGuide_2014
- Sample_Lesson.pdf
- sensor-development-fall compressed
- Lab4 VBA
- 18426 Minimizing Interlaboratory Deviations in the Molec
- Process Calculations 2nd ed (PHI, 2011).pdf
- Colored Periodic Table
- Lecture 5
- FTIR Determination of Unsaturation Grade & Isomers Fat
- Instruction Manual Pocket Colorimeter
- good
- Dsg AU400 AU640 LISspecs
- reso chem dpp
- Chemical Demonstration Booklet 2016
- ISC 2015 Chemistry Paper 2 Practical Solved
- massielauren 5efinaldraft 04202018
- paper 1 (2).pdf

You are on page 1of 6

SPECTROPHOTOMETRIC METHODS

Submitted to fulfill the assignments in the lecture of Analytical Chemistry

By:

1002326

INTERNATIONAL PROGRAM ON SCIENCE EDUCATION FACULTY OF MATHEMATIC AND SCIENCE EDUCATION INDONESIA UNIVERSITY OF EDUCATION 2012

1|Page

1. No. 1. 2. 3. 4. 5.

Fill the blanks on the table below Concentration Transmittans Absorbans Cell Thickness (C) (T) (A) (b) 2 x 10-5 7.50 x 10-6 1 x 10-4 5 x 10-4 3.21 x 10-5 0.56 0.70 0.30 3.16 x 10-3 0.35 0.25 0.15 0.52 2.50 0.45 1 2 1 1 2 Molar Absorptivity ( ) 12,500 10,000 5200 5000 7000

Calculation Based on Lambert-Beers Law: 1). Known : C = 2 x 10-5 mol/L T = 0.56 b = 1 cm : A = -log T A = -log 0.56 A = 0.25

Answer

2).

Known

: T = 0.70 b = 2 cm

Asked : a) C? b) A?

Answer

3).

Known

: C = 1 x 10-4 A = 0.52 b = 1 cm

Asked : a) T? b)

2|Page

Answer

T = 0.30

4) Known

: C = 5 x 10-4 b = 1 cm

Answer

A = - log T 2.5 = - log T T = 3.16 x 10-3 For the absorbance in number 4 I think it is impossible because the maximum value of Absorbance is 1. So there must be wrong number in the Concentration (C). Probably, it should be C = 5 x 10-5 Alternative answer Known : C = 5 x 10-5 M b = 1 cm

Answer

5). Known

: A = 0.45 b = 2 cm

3|Page

Answer

T = 0.35

2. 1 gram of sample that contain Ti(IV) is dissolved into 100 ml of solution is precisely. 10 ml of the solution is pipetted in to 100 ml measurement flask (solution 1), and 10 ml complexing agent to have the red solution. It is diluted until 100 ml (solution 2). The measurement of absorbans in 550 nm is 0.5000 (b = 2 cm, ). a. Calculate the %T of the solution 2 b. Calculate how many ppm of Ti (IV) is in the solution 1 (ppm = mg/L) c. How is the content of Ti (IV) in sample (in % mass / mass)

Known : mass of Ti(IV) (m) = 1 gram in V = 100 ml Solution 1 : 10 ml of Ti(IV) solution + 10 ml of complex agent Solution 2 : V2 = 100 ml A = 0.50 b = 2 cm ).

Asked : a) % T of solution 2? b) ppm of Ti? c) content of Ti (in % mass / mass) Answer : a). A = - log T 0.50 = - log T T = 0.3162 T = 31.62 % Solution of Ti (IV) with 1 gr of mass in 100 ml (Ar of Ti(IV) = 47.87 gr/mol

4|Page

Solution 1 ,

V1 = 10 ml V2 = 10 ml V total = V1 + V2 = 20 ml

b). ppm of Ti (IV) in solution 1 (1 ppm = 1 mg/L) mass of Ti (IV) = 1 gr volume of solution 1 = 20 ml ppm of solution 1 = Solution 2 (from solution 1 then diluted until 100 ml) M1 . V1 = M2 . Vtotal . 20 ml = M2 . 100 ml

Mass of Ti(IV) in solution (m) = n . Ar of Ti m= . 47.87 gr/mol m = 1.00527 gram % content of Ti(IV) % content of Ti(IV) % content of Ti(IV) % content of Ti(IV) % %

3. The 1x10-5 M of solution A has an absorbance as many as 0.350. How is the concentration of solution A that shows 0.75 absorbance if both of solution were measured under the same cell? Known : C of standard solution = 1x10-5 M A of standard solution = 0.35 A of sample solution = 0.75 Asked : C of sample solution?

5|Page

Answer :

Based on Lambert-Beers Law: See that the relation between Absorbance and Concentration is proportional, which means A .

under the same cell so molar absorptivity and the cell thickness must be same, thats why it may eliminate each other. From the equation above, the equation will be:

If it is plotted to the graph, it can be seen that higher concentration or more saturated solution means absorb lights in higher intensity.

0.8

0.7

Absorbance (A)

0.6

0.5

0.4

Concentration (C)

6|Page

- epc621_2Uploaded bysouvik5000
- troy ccr2017Uploaded byapi-228443302
- Calculation Cstr 40lUploaded byhanissuhada
- Shimadzu_CuvetteGuide_2014Uploaded byElizabeth Carr
- Sample_Lesson.pdfUploaded byΔημητρηςΣαρακυρου
- sensor-development-fall compressedUploaded byapi-383164719
- Lab4 VBAUploaded byJonahJunior
- 18426 Minimizing Interlaboratory Deviations in the MolecUploaded byImaPratiwi
- Process Calculations 2nd ed (PHI, 2011).pdfUploaded byArmin Buljubašić
- Colored Periodic TableUploaded byArnav Gupta
- Lecture 5Uploaded byggriff24
- FTIR Determination of Unsaturation Grade & Isomers FatUploaded byrajdewaan
- Instruction Manual Pocket ColorimeterUploaded bymonelu
- goodUploaded byShuraik Kader
- Dsg AU400 AU640 LISspecsUploaded byOrelvi Santos
- reso chem dppUploaded bySubham Yadav
- Chemical Demonstration Booklet 2016Uploaded byTak Man Tung
- ISC 2015 Chemistry Paper 2 Practical SolvedUploaded byUtsyo Chakraborty
- massielauren 5efinaldraft 04202018Uploaded byapi-396013327
- paper 1 (2).pdfUploaded byAbhinav Anand
- Notes 5 Reactive System 1Uploaded byHemant K Patil
- IJSO-14Uploaded byDeepanshu
- Experiment4_1211_Sp06Uploaded byFemina Argonza
- Chap01.pdfUploaded byMarike Bunga Harfintana
- 02 Dasar2 Perancangan Chp1 FoglerUploaded byAdun Dudun
- contoh perhitungan unit prosesUploaded byChristopher Handino Koesno
- Answer Key Stoicometri - I.pdfUploaded bygujarat royal
- Rm Catalogue 161006 0Uploaded byDumitru Marius
- Volumetric SolutionsUploaded byssnoorm
- Kadar Glucose WidUploaded byWidyah

- Uts Fisika Kelas Xi Semester 2Uploaded byMuhammad Rifqi Rofiuddin
- Monocot vs. DicotUploaded byMuhammad Rifqi Rofiuddin
- Modul Fisika - Intensitas BunyiUploaded byMuhammad Rifqi Rofiuddin
- Additives Unit TestUploaded byMuhammad Rifqi Rofiuddin
- Modul Fisika Kelas XiiUploaded byMuhammad Rifqi Rofiuddin
- Silabus IPA SMP 2017.pdfUploaded bylimpatp
- UTS FISIKA KELAS XI SEMESTER 2.pdfUploaded byMuhammad Rifqi Rofiuddin
- Ulangan MomentumUploaded byMuhammad Rifqi Rofiuddin
- Modul Fisika Persiapan UsbnUploaded byMuhammad Rifqi Rofiuddin
- 573-1286-1-SMUploaded byFauzanisme Yo Aq
- Fgd Second MeetingUploaded byMuhammad Rifqi Rofiuddin
- Peta KonsepUploaded byMuhammad Rifqi Rofiuddin
- 2. Kata Pengantar Daftar IsiUploaded byMuhammad Rifqi Rofiuddin
- 64 Interview QuestionsUploaded byshivakumar N
- absUploaded byMuhammad Rifqi Rofiuddin
- Proposal MAULID NABIUploaded byMuhammad Rifqi Rofiuddin
- ELASTISITASUploaded byMuhammad Rifqi Rofiuddin
- 12 Factors Influencing Friction Force (TM)Uploaded byMuhammad Rifqi Rofiuddin
- Plant AdaptationUploaded byMuhammad Rifqi Rofiuddin
- 10 Nasihat Bijak EinsteinUploaded byMuhammad Rifqi Rofiuddin
- CoverUploaded byMuhammad Rifqi Rofiuddin

- Calibration CurveUploaded byGulam Dastgir Quraishi
- Acid Dissociation Constant of Methyl RedUploaded byNece Jean Tagam
- is.5182.02.2001-1Uploaded byKrishna Srikanth
- Simultaneous Spectrophotometric DeterminationUploaded byMauricio Diez
- Ultraviolet VisibleUploaded byMXR-3
- WBCUploaded byIsmaela Catipay
- Espec_VisionLite_opmanUploaded bybarlos
- lab 3Uploaded byapi-383615212
- kinetiUploaded byanungdroid
- Lab 457-4Uploaded byDavid Little
- Pierce Micro BCA Protein AssayUploaded byTim Charlton
- As 1050.6 - 1995 Methods for the Analysis of Iron and Steel - Determination of Tin Content - Flame Atomic Absorption Spectrometric MethodUploaded bynicu1212
- Quality Control Lab Manual AnswersUploaded bykriss Wong
- 6 Vai Final r n dUploaded byFree Escort Service
- Linear IzationUploaded byBiswajitMaity
- APPLICATIONS OF SIMULTANEOUS EQUATION METHOD FOR THE DETERMINATION OF EMTRICITABINE AND TENOFOVIR DISOPROXIL FUMARATE TO DISSOLUTION STUDIES IN MARKETED TABLETSUploaded byBaru Chandrasekhar Rao
- chemistry practicalUploaded byMukhtar Malik
- DR 5000 MaintananceUploaded byEko Yudie Setyawan
- gümüş nano partikülUploaded bykrdr_281987
- CHEM F110 -First Six experiments.pdfUploaded byRahul Shivroop
- Biotech Lab 1 ReportUploaded byAmirul Izwan
- Cary 100 UV-Vis Operating InstructionsUploaded byBassem Ahmed
- Curs_hplc.pptUploaded byPishta Bmc
- Product DetailsUploaded bySandeep Mishra
- SIAM (Losartan +Hydrochlorthiazide)Uploaded byRavi Kant
- AA-6300Uploaded byCGFernandez2014
- OPTICAL METHOD OF ANALYSISUploaded byJohn Kevin San Jose
- 9 Light Absorption Emission Scattering Beer LambertUploaded byProf.Dr.Mohamed Fahmy Mohamed Hussein
- 1005-Article Text-1729-1-10-20131005Uploaded byMarcelo Alexander Guancha Chalapud
- report2Uploaded byapi-253994289