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# EXERCISE

SPECTROPHOTOMETRIC METHODS
Submitted to fulfill the assignments in the lecture of Analytical Chemistry

By:

## Arranged by: Muhammad Rifqi Rofiuddin

1002326

INTERNATIONAL PROGRAM ON SCIENCE EDUCATION FACULTY OF MATHEMATIC AND SCIENCE EDUCATION INDONESIA UNIVERSITY OF EDUCATION 2012
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1. No. 1. 2. 3. 4. 5.

Fill the blanks on the table below Concentration Transmittans Absorbans Cell Thickness (C) (T) (A) (b) 2 x 10-5 7.50 x 10-6 1 x 10-4 5 x 10-4 3.21 x 10-5 0.56 0.70 0.30 3.16 x 10-3 0.35 0.25 0.15 0.52 2.50 0.45 1 2 1 1 2 Molar Absorptivity ( ) 12,500 10,000 5200 5000 7000

Calculation Based on Lambert-Beers Law: 1). Known : C = 2 x 10-5 mol/L T = 0.56 b = 1 cm : A = -log T A = -log 0.56 A = 0.25

## and A = - log T Asked : a). b). A?

2).

Known

: T = 0.70 b = 2 cm

Asked : a) C? b) A?

## : A = -log T A = -log 0.70 A = 0.15

3).

Known

: C = 1 x 10-4 A = 0.52 b = 1 cm

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## : A = - log T 0.52 = - log T

T = 0.30

4) Known

: C = 5 x 10-4 b = 1 cm

## Asked : a). T? b). A?

A = - log T 2.5 = - log T T = 3.16 x 10-3 For the absorbance in number 4 I think it is impossible because the maximum value of Absorbance is 1. So there must be wrong number in the Concentration (C). Probably, it should be C = 5 x 10-5 Alternative answer Known : C = 5 x 10-5 M b = 1 cm

## A = - log T 0.25 = - log T T = 0.56

5). Known

: A = 0.45 b = 2 cm

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## : A = -log T 0.45 = -log T

T = 0.35

2. 1 gram of sample that contain Ti(IV) is dissolved into 100 ml of solution is precisely. 10 ml of the solution is pipetted in to 100 ml measurement flask (solution 1), and 10 ml complexing agent to have the red solution. It is diluted until 100 ml (solution 2). The measurement of absorbans in 550 nm is 0.5000 (b = 2 cm, ). a. Calculate the %T of the solution 2 b. Calculate how many ppm of Ti (IV) is in the solution 1 (ppm = mg/L) c. How is the content of Ti (IV) in sample (in % mass / mass)

Known : mass of Ti(IV) (m) = 1 gram in V = 100 ml Solution 1 : 10 ml of Ti(IV) solution + 10 ml of complex agent Solution 2 : V2 = 100 ml A = 0.50 b = 2 cm ).

Asked : a) % T of solution 2? b) ppm of Ti? c) content of Ti (in % mass / mass) Answer : a). A = - log T 0.50 = - log T T = 0.3162 T = 31.62 % Solution of Ti (IV) with 1 gr of mass in 100 ml (Ar of Ti(IV) = 47.87 gr/mol

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Solution 1 ,

V1 = 10 ml V2 = 10 ml V total = V1 + V2 = 20 ml

## M1 . V1 = M2 . Vtotal 0.21 mol/ml . 10 ml = M2 . 20 ml

b). ppm of Ti (IV) in solution 1 (1 ppm = 1 mg/L) mass of Ti (IV) = 1 gr volume of solution 1 = 20 ml ppm of solution 1 = Solution 2 (from solution 1 then diluted until 100 ml) M1 . V1 = M2 . Vtotal . 20 ml = M2 . 100 ml

Mass of Ti(IV) in solution (m) = n . Ar of Ti m= . 47.87 gr/mol m = 1.00527 gram % content of Ti(IV) % content of Ti(IV) % content of Ti(IV) % content of Ti(IV) % %

3. The 1x10-5 M of solution A has an absorbance as many as 0.350. How is the concentration of solution A that shows 0.75 absorbance if both of solution were measured under the same cell? Known : C of standard solution = 1x10-5 M A of standard solution = 0.35 A of sample solution = 0.75 Asked : C of sample solution?

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Based on Lambert-Beers Law: See that the relation between Absorbance and Concentration is proportional, which means A .

under the same cell so molar absorptivity and the cell thickness must be same, thats why it may eliminate each other. From the equation above, the equation will be:

If it is plotted to the graph, it can be seen that higher concentration or more saturated solution means absorb lights in higher intensity.

0.8

0.7

Absorbance (A)

0.6

0.5

0.4

## 0.3 0.000010 0.000012 0.000014 0.000016 0.000018 0.000020 0.000022

Concentration (C)

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