Guidelines for Working in the Dept of Bioengineering Microscopy Labs

1) Only REGISTERED USERS are permitted to use the lab. To be registered, you need to read and sign this document, leaving 1 copy with Peter Sowinski. You may not induct other people or give them permission to work in the lab. 2) You must not bring coats, bags, scarves, etc. into the lab. They may be left at owner’s risk in the anteroom to the cell culture lab. 3) A numbered lab coat will be allocated to you and must be worn at all times while in the lab. When the lab coat is dirty, send it for washing by placing it in the allocated bin in the store room (B626) and use another. Hang lab coats on coat hooks, not chairs, when not needed. It is hazardous to wear open-toed shoes or sandals. 4) When handling chemicals, you must also wear gloves. Used gloves should be placed in the yellow/orange bags. Your Risk Assessments should detail what other protective equipment is required. 5) It is absolutely forbidden to eat, drink, apply make-up or use a mobile phone in the lab. Avoid chewing pens, pencils, etc. 6) Spillages must be cleaned up immediately and appropriately. 7) If you wish to store samples or solutions in the lab, then you must label the container with your name, the contents, date and, if appropriate, the expiry date. Please use a piece of tape to label instead of labelling the container directly (so that once you have finished with the bottle, someone else can use it). Unlabelled samples or solutions will be disposed of. 8) Once you have finished your work, tidy up the bench and wash your hands before you leave the lab. 9) Only trained personnel may use the confocal microscope. Training for confocal microscopy consists of an hour on-line lecture (Confocal Link) by Peter Weinberg, followed by a hands on training session on the microscope provided by Ken Keating. The fluorescence microscope must be used in accordance with its instructions; in particular, the correct sequence of switching on and off the camera and mercury arc lamp must be followed. 10) Mercury arc lamps must not be used past their rated life. If a mercury arc lamp explodes, releasing mercury vapour into the room, the room should be evacuated and the door closed; the room should not be re-entered until the ventilation system has had time to remove contaminated air. 11) If you notice any faulty equipment or any other problems in the lab and you cannot resolve the problem, please notify one of us. 12) In addition to completing this induction, other paperwork is required before work in the laboratory can commence. Page 1 of 3

ac.ac.ac. (d) To use a chemical (including chemicals already in the lab. to 6 p. 17) Gross safety violations (e.2). or working without a Risk Assessment) may result in a ban from the lab. For disposal of full yellow/orange waste bags. first aid kits. then your name will be added to the cleaning rota. 16) If you are a regular user of the microscopy lab. Monday to Friday. endangering other people. which you have not purchased). 14) Normal working hours are 8 a.g.uk with your name and your supervisor’s name. (c) You must have completed the Departmental Safety Registration process. When working alone outside these hours.uk Peter: p. 13) The following may not be used in the lab: (a) radioactive isotopes. the guidance on lone working given in section 13 of the Departmental Safety Booklet must be followed.m. Unfixed human cells and tissues may need special designated areas and therefore should not be used without consulting Peter Weinberg.uk/bioengineering/about/safety). 15) Equipment left running unattended must be labelled with a note stating the experimenter’s name. you must complete a COSHH assessment unless it is stated in the MSDS that the chemical is nonhazardous to heath.weinberg@imperial.keating@imperial. Undergraduate students may not normally perform experimental work outside timetabled hours (section 4.m. (b) You must have completed the Department’s Health and Safety Induction.sowinski@imperial.ac. Contact details: Peter-Piotr: p.imperial. The Risk Assessment needs to contain details of emergency telephones.ac. (b) biological agents that could cause serious or very serious human or animal disease.uk Ken: k. Further information on cleaning rota will be provided during the induction. contact telephone number and expected end time/date. (e) A Risk Assessment must be completed and followed for each procedure.uk Page 2 of 3 . requesting access to the waste disposal area and the lift in Bessemer Building.(a) You must have read the Departmental Safety Booklet (http://www3.card@imperial. please email id. escape routes and fire extinguishers in the vicinity. eating or drinking in the lab. and (c) procedures regulated under the Animals (Scientific Procedures) Act 1986.

...................... Name & Signature....................11 Page 3 of 3 ........... Authorised by.......................................................... Date...................................11. Date.................... mobile: 07590 250 478................... I certify that I have read and understood the information provided to me and will abide by laboratory and departmental rules/policies when using these laboratories. Professor P D Weinberg 1........Emergency contact numbers: Peter Weinberg 020 7594 1517 (work) 020 8755 4660 (home) Ken Keating 020 7594 5170.....................