MEKANISME KERJA ENZIM

Molekul selalu bergerak dan bertumbukan satu dengan yang lain. Jika suatu molekul substrat
menumbuk molekul enzim yang tepat, substrat akan menempel pada enzim. Tempat menempelnya
molekul substrat pada enzim disebut dengan sisi aktif. Kemudian terjadi reaksi dan terbentuk
molekul produk.
Banyak enzim yang dapat bekerja bolak-balik (reversible). Enzim dapat mengubah substrat
menjadi hasil akhir dan juga dapat mengubah hasil akhir menjadi substrat jika lingkungannya
berubah. Misalnya, enzim lipase dapat berfungsi katalisator dalam perubahan lemak menjadi asam
lemak dan glilserol. Enzim lipase juga dapat mengubah kembali asam lemak dan gliserol menjadi
lemak (lipid).
Enzim juga bekerja secara spesifik, artinya enzim mempunyai fungsi yang khusus. Jika enzim
berbeda maka hasilnya akan berbeda pula. Misalnya, pemecahan rafinosa (suatu trisakarida). Jika
dilakukan oleh enzim sukrase rafinosa akan terurai menjadi melibiosa dan fruktosa, sedangkan jika
dilakukan dengan oleh enzim emulsion rafinosa akan terurai menjadi sukrosa dan galaktosa.
Ada dua teori mengenai mekanisme kerja enzim, yaitu lock and key theory dan induced fit theory.
1) Lock and Key Theory (Teori Gembok dan Kunci)
Teori ini dikemukakan oleh Fischer (1988). Menurutnya, enzim diumpamakan sebagai gembok
karena memiliki sebuah bagian kecil yang dapat berikatan dengan substrat yang disebut dengan sisi
aktif, sedangkan substrat sebagai kunci karena dapat berikatan secara pas dengan sisi aktif enzim.
Substrat dapat berikatan dengan enzim jika sesuai dengan sisi aktif enzim. Sisi aktif enzim
mempunyai bentuk tertentu yang hanya sesuai untuk satu jenis substrat saja, hal itu menyebabkan
enzim bekerja secara spesifik. Substrat yang mempunyai bentuk ruang yang sesuai dengan sisi
aktif enzim akan berikatan dan membentuk kompleks transisi enzim-substrat. Senyawa transisi ini
tidak stabil sehingga pembentukan produk berlangsung dengan sendirinya. Jika enzim mengalami
denaturasi (rusak) karena panas, bentuk sisi aktif akan berubah sehingga substrat tidak sesuai lagi.
Perubahan pH juga mempunyai pengaruh yang sama.
2) Induced Fit Theory (Teori Ketepatan Induksi)
Teori ini dikemukakan oleh Daniel Koshland. Menurutnya, sisi aktif enzim bersifat fleksibel.
Akibatnya, sisi aktif enzim dapat berubah bentuk menyesuaikan bentuk substrat. Teori ini sesuai
dengan mekanisme kerja enzim yangt sesungguhnya.
Reaksi antara substrat dengan enzim berlangsung karena adanya induksi molekul substrat terhadap
molekul enzim. Menurut teori ini, sisi aktif enzim bersifat fleksibel dalam menyesuaikan stuktur
sesuai dengan struktur substrat. Ketika substrat memasuki sisi aktif enzim, maka enzim akan
terinduksi dan kemudian mengubah bentuknya sedikit sehingga mengakibatkan perubahan sisi
aktif yang semula tidak cocok menjadi cocok (fit). Kemudian terjadi pengikatan substrat oleh
enzim yang selanjutnya substrat diubah menjadi produk. Produk kemudian dilepaskan dan enzim
kembali pada keadaan semula dan siap untuk mengikat substrat baru.
Diposkan oleh vhavha di 17:

Cara Kerja (Mekanisme) Enzim- Sifat khusus enzim lainnya adalah tidak ikut bereaksi, artinya
enzim hanya memproses substrat (contohnya, lemak) menjadi produk (contohnya, gliserol dan

asam lemak) tanpa ikut mengalami perubahan dalam reaksi itu. Bahan tempat kerja enzim disebut
substrat dan hasil dari reaksi disebut produk. Dengan demikian enzim dapat digunakan kembali
untuk mengkatalisis reaksi yang sama, berikutnya. Mekanisme kerja enzim dapat Anda lihat pada
Gambar 2.3.
Gambar 2.3 Hubungan enzim dan substrat
Secara sederhana cara kerja enzim dapat digambarkan dengan kunci dan gembok. Kompleks enzim
dapat tumbuh pada substrat karena pada permukaan enzim terdapat sisi aktif. Sisi aktif tersebut
mempunyai konfigurasi aktif tertentu dan hanya substrat tertentu yang dapat bergabung dan
menyebabkan enzim dapat bekerja secara spesifik. Secara sederhana reaksi enzim dituliskan:
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Sifat-sifat enzim selain sebagai biokatalisator dan sebagai suatu protein, enzim mempunyai sifat
yaitu berperan tidak bolak-balik. Artinya enzim dapat bekerja menguraikan suatu substrat menjadi
substrat tertentu dan tidak sebaliknya dapat menyusun substrat sumber dari hasil penguraian,
misalya enzim protease dapat menguraikan protein menjadi asam amino, tetapi tidak
menggabungkan asam aminonya menjadi protein.
Enzim menjadi rusak apabila berada pada suhu yang terlalu panas atau terlalu dingin. Sebagian
besar enzim akan rusak pada suhu di atas 60ºC karena proteinnya (gugus prostetik) menggumpal
(koagulasi). Jika telah rusak maka tidak akan berfungsi lagi meskipun berada pada suhu normal,
rusaknya enzim oleh panas disebut denaturasi. Selain itu, kerja enzim juga dapat terhalang oleh zat
lain. Zat yang dapat menghambat kerja enzim disebut inhibitor, contohnya CO, Arsen, Hg, dan
Sianida. Sebaliknya zat yang dapat mempercepat jalannya reaksi disebut aktivator, contohnya ion
Mg²+, Ca²+, zat organik seperti koenzim-A.
Enzim dapat bekerja optimal pada pH tertentu, misalnya enzim lipase, pH optimal 5,7–7,5.
Aplikasi pH yang tidak cocok maka sifat kerja enzim dapat menyebabkan ionisasi dari gugus
karboksil dan amino dari bagian-bagian enzim yang tersusun atau apoenzim dan dapat
menyebabkan denaturasi, oleh karena itu akan terjadi tambahan struktur enzim sehingga tidak

dapat bekerja dengan baik.

MEKANISME KERJA ENZIM
Mekanisme Enzim dapat bekerja dengan beberapa cara, yang kesemuaannya
menurunkan ΔG‡:[26] Menurunkan energi aktivasi dengan menciptakan suatu
lingkungan yang mana keadaan transisi terstabilisasi (contohnya mengubah
bentuk substrat menjadi konformasi keadaan transisi ketika ia terikat dengan
enzim.) Menurunkan energi keadaan transisi tanpa mengubah bentuk substrat dengan
menciptakan lingkungan yang memiliki distribusi muatan yang berlawanan dengan keadaan
transisi. Menyediakan lintasan reaksi alternatif. Contohnya bereaksi dengan substrat sementara
waktu untuk membentuk kompleks Enzim-Substrat antara. Menurunkan perubahan entropi reaksi
dengan menggiring substrat bersama pada orientasi yang tepat untuk bereaksi. Menariknya, efek
entropi ini melibatkan destabilisasi keadaan dasar,[27] dan kontribusinya terhadap katalis relatif
kecil.[28] Cara enzim bekerja adalah dengan membentuk senyawa enzim-substrat, kemudian
menghasilkan suatu produk tanpa merubah senyawa enzim itu sendiri, setelah produk terbentuk
maka enzim akan melepaskan diri untuk membentuk senyawa baru dengan substrat yang lain. Ada
2 (dua) cara kerja enzim : Lock and key (gembok dan anak kunci) Setiap enzim memiliki sisi aktif
yang tersusun dari sejumlah asam amino. Bentuk sisi aktif ini sangat spesifik, sehingga hanya
molekul dengan bentuk tertentu yang dapat menjadi substrat bagi enzim. Induced fit (induksi pas)
Sisi aktif enzim merupakan bentuk yang tidak kaku (fleksibel). Ketika substrat memasuki sisi aktif
enzim, bentuk sisi aktif berubah bentuk sesuai dengan bentuk substrat kemudian terbentuk
kompleks enzim-substrat. Pada saat produk sudah terlepas dari kompleks, maka enzim lepas dan
kembali bereaksi dengan substrat yang lain. Enzim bekerja dengan cara mengkatalis reaksi
sehingga meningkatkan kecepatan reaksi yang dilakukan dengan menurunkan energi aktivasi
(energi yang dibutuhkan untuk reaksi). MEKANISME KERJA ENZIM Molekul selalu bergerak
dan bertumbukan satu dengan yang lain. Jika suatu molekul substrat menumbuk molekul enzim
yang tepat, substrat akan menempel pada enzim. Tempat menempelnya molekul substrat pada
enzim disebut dengan sisi aktif. Kemudian terjadi reaksi dan terbentuk molekul produk. Banyak
enzim yang dapat bekerja bolak-balik (reversible). Enzim dapat mengubah substrat menjadi hasil
akhir dan juga dapat mengubah hasil akhir menjadi substrat jika lingkungannya berubah. Misalnya,
enzim lipase dapat berfungsi katalisator dalam perubahan lemak menjadi asam lemak dan glilserol.
Enzim lipase juga dapat mengubah kembali asam lemak dan gliserol menjadi lemak (lipid). Enzim
juga bekerja secara spesifik, artinya enzim mempunyai fungsi yang khusus. Jika enzim berbeda
maka hasilnya akan berbeda pula. Misalnya, pemecahan rafinosa (suatu trisakarida). Jika dilakukan
oleh enzim sukrase rafinosa akan terurai menjadi melibiosa dan fruktosa, sedangkan jika dilakukan
dengan oleh enzim emulsion rafinosa akan terurai menjadi sukrosa dan galaktosa. Ada dua teori
mengenai mekanisme kerja enzim, yaitu lock and key theory dan induced fit theory. 1) Lock and
Key Theory (Teori Gembok dan Kunci) Teori ini dikemukakan oleh Fischer (1988). Menurutnya,

enzim diumpamakan sebagai gembok karena memiliki sebuah bagian kecil yang dapat berikatan
dengan substrat yang disebut dengan sisi aktif, sedangkan substrat sebagai kunci karena dapat
berikatan secara pas dengan sisi aktif enzim. Substrat dapat berikatan dengan enzim jika sesuai
dengan sisi aktif enzim. Sisi aktif enzim mempunyai bentuk tertentu yang hanya sesuai untuk satu
jenis substrat saja, hal itu menyebabkan enzim bekerja secara spesifik. Substrat yang mempunyai
bentuk ruang yang sesuai dengan sisi aktif enzim akan berikatan dan membentuk kompleks transisi
enzim-substrat. Senyawa transisi ini tidak stabil sehingga pembentukan produk berlangsung
dengan sendirinya. Jika enzim mengalami denaturasi (rusak) karena panas, bentuk sisi aktif akan
berubah sehingga substrat tidak sesuai lagi. Perubahan pH juga mempunyai pengaruh yang sama.
2) Induced Fit Theory (Teori Ketepatan Induksi) Teori ini dikemukakan oleh Daniel Koshland.
Menurutnya, sisi aktif enzim bersifat fleksibel. Akibatnya, sisi aktif enzim dapat berubah bentuk
menyesuaikan bentuk substrat. Teori ini sesuai dengan mekanisme kerja enzim yangt
sesungguhnya. Reaksi antara substrat dengan enzim berlangsung karena adanya induksi molekul
substrat terhadap molekul enzim. Menurut teori ini, sisi aktif enzim bersifat fleksibel dalam
menyesuaikan stuktur sesuai dengan struktur substrat. Ketika substrat memasuki sisi aktif enzim,
maka enzim akan terinduksi dan kemudian mengubah bentuknya sedikit sehingga mengakibatkan
perubahan sisi aktif yang semula tidak cocok menjadi cocok (fit). Kemudian terjadi pengikatan
substrat oleh enzim yang selanjutnya substrat diubah menjadi produk. Produk kemudian dilepaskan
dan enzim kembali pada keadaan semula dan siap untuk mengikat substrat baru. PENGARUH
INHIBITOR TERHADAP KERJA ENZIM Aktivitas dari enzim dapat dipengaruhi oleh beberapa
jenis molekul, salah satunya adalah inhibitor. Inhibitor merupakan suatu senyawa yang dapat
menghambat atau menurunkan laju reaksi yang dikatalisis oleh enzim. Inhibitor irreversibel atau
tidak dapat balik, dimana setelah inhibitor mengikat enzim, inhibitor tidak dapat dipisahkan dari
sisi aktif enzim. Keadaan ini menyebabkan enzim tidak dapat mengikat substrat atau inhibitor
merusak beberapa komponen (gugus fungsi) pada sisi katalitik molekul enzim. Sedangakan
nhibitor reversibel atau dapat balik, bekerja dengan mengikat sisi aktif enzim melalui reaksi
reversibel dan inhibitor ini dapat dipisahkan atau dilepaskan kembali dari ikatannya. Inhibitor
dapat balik terdiri dari tiga jenis, yaitu inhibitor yang bekerja secara kompetitif, non-kompetitif,
dan un-kompetitif. Beberapa jenis molekul dapat mempengaruhi aktivitas enzim. Aktivitas dari
enzim dapat dipengaruhi oleh beberapa jenis molekul, salah satunya adalah inhibitor. Inhibitor
merupakan suatu senyawa yang dapat menghambat atau menurunkan laju reaksi yang dikatalisis
oleh enzim. Inhibitor irreversibel atau tidak dapat balik, dimana setelah inhibitor mengikat enzim,
inhibitor tidak dapat dipisahkan dari sisi aktif enzim. Keadaan ini menyebabkan enzim tidak dapat
mengikat substrat atau inhibitor merusak beberapa komponen (gugus fungsi) pada sisi katalitik
molekul enzim. Sedangakan nhibitor reversibel atau dapat balik, bekerja dengan mengikat sisi aktif
enzim melalui reaksi reversibel dan inhibitor ini dapat dipisahkan atau dilepaskan kembali dari
ikatannya. Inhibitor dapat balik terdiri dari tiga jenis, yaitu inhibitor yang bekerja secara
kompetitif, non-kompetitif, dan un-kompetitif.

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