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Name:

LAB REPORT #1: Safety + Biological Molecules


BIOLOGY I for the Pre-Health Sciences – Durham College, Fall 2018

/25 marks
EVALUATION NOTES

Read before submitting:

• The pre-lab quiz and lab report are worth 5% of your


final mark.

• This lab report is due by 11:59 pm on the due date


established by your instructor.

a. A report submitted after this due date is


considered late and will result in a grade of 0
unless a late submission is negotiated.
b. A negotiated late submission is a lab report
that has been submitted late for valid reasons
with the written permission of the instructor.
It is the responsibility of the student to
request permission to negotiate a late
submission no later than 48 hours prior to the
due date. Late submissions are penalized
10% per day up to a max of 3 days (including
weekends). After that a zero is assigned.

• This lab report should be submitted online to the DC Connect Assignments folder.

a. The report should be submitted using a fillable PDF available on DC Connect.


An instructional video is posted on how to use a fillable PDF (or click here).
Unless otherwise directed, submissions made by other means (E.g. email or
hard copy) will not be accepted and will result in a grade of 0.
b. Computer malfunctions or technical issues are not accepted as excuses for late
or missing reports. TIP: Submit your report early so that you have time to
resolve any potential technical issues prior to the deadline.

• While students work in pairs to complete labs, each student must submit their own
original work.

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PART 1 – LAB SAFETY

1. (1 mark) Submit a photo of the labeled laboratory map you created (p. 11 in lab
manual).
TIP 1: Crop/zoom your image if necessary so that it is clear to the viewer.
TIP 2: Make sure the fillable PDF is open in Adobe when inserting photos. It will not
work if you are viewing the PDF in an internet browser. If you are having trouble
attaching your photo, never fear! Just watch the help video on DC Connect or contact
your instructor.

2. Imagine that your lab partner removed their safety glasses in the lab and an unknown
chemical splashed in their eyes.
a. (1 mark) What are suitable steps to take? Check ALL that apply.
 Inform the lab instructor
 Keep calm
 Scream for help
 Ask your lab partner if they are OK
b. (1 mark) Using the map in Question 1, describe where you would find relevant
safety equipment that would aid you during this incident.

3. (2 marks) What were the 2 common safety mistakes made in Image C of Table 1.2
(p. 13 of lab manual)?

Mistake 1: _____________________________________________________
Mistake 2: _____________________________________________________

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PART 2 – BIOLOGICAL MOLECULES

4. In the chart below, submit your photos of the models that you built in the lab.

a. (1 mark) CARBOHYDATES – p. 15 of lab manual

Monosaccharide Disaccharide Polysaccharide

b. (2 marks) PROTEIN – p. 18 of lab manual


Polypeptide Polypeptide
Amino acid
– primary – secondary

Polypeptide Polypeptide
– tertiary – quaternary

c. (2 marks) LIPID – p. 21 of lab manual

Triglyceride Fat vs Oil Phospholipid

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5. (4 marks) Fill in the blanks using information from your models and the lab manual
(p. 15, 18, 21)

a. The monomer of a carbohydrate is the ________.


b. An example of a disaccharide is ________.
c. 3+ monosaccharides joined together is referred to as a ________.
d. The monomer of a protein is the ________.
e. A polypeptide bonded to other polypeptides is said to have ________
structure.
f. A triglyceride consists of glycerol bonded to 3 ________.
g. A fatty acid with all ________ bonds is considered to be saturated.
h. A ______-mer is the building block of many biological molecules.

6. (2 marks) Compare and contrast the structure of a FAT vs an OIL by listing 2


similarities and 2 differences:

Similarities:

• ________________________________________________________

• ________________________________________________________

Differences:

Fat Oil

Vs

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7. (5 marks) Fill-in the following chart by placing a “Y” or “N” in each box below. Be
sure to base your answers off of evidence collected in Tables 1.3 - 1.5 of the lab
manual (p. 17, 20, 23).
Y = The biological molecule is present in that particular test sample.
N = The biological molecule is NOT present in that particular test sample.

BENEDICT LUGOL BUIRET SUDAN

Mono or di- Poly- Amino Poly- Tri-


Test
saccharides saccharides acids peptides glycerides

1
glucose
(monosaccharide)

2
starch
(polysaccharide)

3
vegetable oil
(triglyceride)

4
albumin
(polypeptide)

5
chicken broth

6
mayonnaise

7
banana

8
potato

9
water

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8. (2 marks) Nutrition labeling became mandatory in Canada for all prepackaged foods
on December 12, 2007. Below is the nutritional label for a popular brand of
mayonnaise.

a. Compare your experimental results in the


previous question to this label. Do you
consider this label to be accurate? IN
YOUR OWN WORDS, describe at least 2
reasons why you think this.

[Source]

9. Imagine that you are a nurse working at Toronto General Hospital in Toronto, ON.
You are currently helping to treat 3 patients who are suffering from a variety of
different metabolic disorders. These patients are on strict diets to help control the
symptoms of their disorder.
Patient A: Lactose intolerant – unable to metabolize lactose
Patient B: Gaucher’s disease – unable to metabolize fat
Patient C: Phenylketonuria – unable to break down certain proteins,
specifically those containing the amino acid phenylalanine
A friend of yours who is a chef in the local cafeteria has been making a homemade
soup and has asked you if it would be suitable for your patients’ diets. As it tastes
delicious, you decide to test it to see what biological molecules are present. Here are
the results that you obtain:
Benedict’s test: Solution remains blue
Lugol’s test: Solution turns navy blue
Biuret’s test: Solution turns from blue to violet
Sudan test: Solution contains red droplets

a. (1 mark) According to the results, which biological molecules are present in this
soup? Check ALL that apply.
 Monosaccharid  Polysaccharide  Polypeptide
 Disaccharide  Amino acid  Triglyceride

b. (1 mark) Who would be able to eat this soup safely? Check ALL that apply.
 Patient A  Patient B  Patient C

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