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Isolation endophytic Streptomyces from Thai Herb and screening for anti-cancer activity from its metabolites

Saharat Jantaratrirat Department of Microbiology, Faculty of Science, Chulalongkorn University

Streptromyces sp.com/10852. http:// • normal flora in soil • Gram’s positive • filamentous bacteria • produce spores • secrete a chemicals called “geosmens” http://aspidisca.livejournal.html .

BIOACTIVE COMPOUND FROM Streptomyces sp.com/science/article/2011-01/scientists-mine-dormant-bacterial-genes-new-antibiotics . • produce more than two-thirds of the clinically used natural antibiotics • an important source of clinically important immunosupressants • source of antitumor agents http://www.popsci.

procto.ro/doxorubicin/ . http://www.Doxorubicin • made by Streptomyces peucetius • commonly used in the treatment of many different cancers.

Endophytic Streptomyces http://aem.expansion.html .asm.org/content/69/7/4260/F1.

cancer cells http://www.edu/depts/our/hhmi/hhmi-ft_learning_modules/cancermodule/pages/cancer.html .unc.

Liver cancer .

V.2001-2003. Vol.Leading cancer in Thailand (estimated).2010 . 2001-2003 ASR=Age-standardized incidence rates Cancer in Thailand.

Leading cancer in Thailand (estimated). Vol.V.2010 . 2001-2003 ASR=Age-standardized incidence rates Cancer in Thailand.2001-2003.

Thai herbs .

Turmeric .

Objectives  to screen Endophytic Streptomyces from turmeric  investigated the effect of Streptomyces ‘s metabolites on the growth of human hepatocarcinoma cell line .

Outline Isolation of Endophytic Streptomyces from turmeric Verification isolated bacteria by 16S rDNA sequence analysis test cytotoxic effects of metabolites on human hepatocarcinoma cell line(HepG2) by MTT assay .

Isolation of Endophytic Streptomyces from turmeric the turmeric were thoroughly washed in running water to remove all soil surface sterilization confirm the surface disinfection by roll plant tissues onto tryptic soy agar and incubate at 30oC plate turmeric fragments on humic acid-vitamin B agar and incubate at 30oC .

2007) 16S rRNA gene amplification by polymerase chain reaction the PCR products were purified using QIAquick PCR Purification Kit sequence 16s rDNA product the sequence data were deposited in GenBank and compared with other Streptomycete gene sequences in GenBank ..Verification isolated bacteria by 16S rDNA sequence analysis extract genomic DNA(Li et al.

test cytotoxic effects of metabolites on human hepatocarcinoma cell line extract metabolites by acetone and concentrate by a rotary vacuum evaporator HepG2 cells are plated in 96-well plate incubate overnight Treat with crude extracts at various concentrations for 4 days Add MTT solution and incubate for 4 hours Add 10%SDS and incubate overnight the absorbance was measured using a microculture plate reader at 540 nm. .

Isolation endophytic Streptomyces from Thai Herb and screening for anti-cancer activity from its metabolites Saharat Jantaratrirat Department of Microbiology. Chulalongkorn University . Faculty of Science.