COMPARATIVE STUDY OF SWETA PARPATI & SHEETAL PARPATI IN MUTRAKRICHRA W.S.
R TO THEIR ANTI MICROBIAL STUDY - BY
DR. NITU SINGH
P.G SCHOLAR DEPARTMENT OF RASA SHASTRA
Dr R S Hiremath. MD
Dept of Rasashastra
Dr P G Jadar. MD
Dept of Rasashastra
K.L.E’s Shri B M K Ayurveda Mahavidyalaya. Belgaum, Karnataka.
NEED FOR STUDY
• There are many formulations told for mutrakrichra in Ayurveda. Shweta parpati and sheetal parpati are two such preparations • It is usually prepared from kajjali or by adding some ingredients to kajjali. • Sweta parpati is nirgandha, and sheetal parpati is sagandha preparation .
• Physical and chemical analysis of raw materials • Shodhana of ingredients and preparation of Shweta parpati and sheetal parpati. • Analytical study of prepared compounds& Comparative evaluation of antimicrobial activity of both formulations on 1) E.coli 2) Proteus mirabilis 3) Staphylococcus aureus 4) Candida albicans
MATERIALS & METHODS
Raw materials were purchased and authentificated. Kalmisora nirmalikarana. Preparation of ela hima. Kalmisora shodhana -- in order to enhance its diuretic property.
Kankshi shodhana. Navsadara shodhana. Preparation of triphala kwatha. Gandhaka shodhana.
Preparation of shweta parpati: Kalmi sora-96 gm shodhita kankshi-12 gm Shodhita navasadar-6gm Preparation of sheetal parpati: Kalmi sora-128gm Shodhita gandhaka-2 gm
ANALYTICAL PROCEDURES QUALITATIVE ANALYSIS
Basic radicals : Na+, K+, NH4+, Al+3, Zn+2, Ba+2, Ca+2 , Mg+2 Acid radicals: Cl- , Br-, So4, No3 may be present in the compounds. Determination of pH valuesIt is noted about 4.19 in shweta parpati & 6.76 in sheetal parpati. by help of ph meter
Material SORA Before Shodhana Pandura Odourless After Shodhana Sikatakara Pandura Karpurabha crystals Smell of Ela Small Opaque
Yellowish White; Transparent Clear White ; Amorphous Hard Crystals ; Guru,Snigdha Soft powder ; Laghu, Ruksha White Crystalline Odourless Greenish yellow Snigdha Hard crystals White Crystalline Odourless Yellow Snigdha Brittle Crystals
Shweta parpati: • Colour: white • Odour: Kshariya • Touch: smooth • Taste: lavana,sheeta • pH: 4.19 • Total ash: 132.8451% • Total chloride: 3.1475% • Total sulphate: 4.0824% • Qualitative test for K+&Al3+: Positive Sheetal parpati: • Colour: light brown • Odour: smell of gandhaka • Touch: rough • Taste: lavana,sheeta • pH: 6.76 • Total ash: 99.1011% • Total sulphates: 0.2141% • Qualitative test for K+ & Fe+3: Positive
E. coli B A
+VE +VE -VE -VE -VE
S. aureus A B
+VE -VE -VE -VE -VE +VE -VE -VE -VE -VE
C. albicans Pr. mirabilis B A A B
-VE -VE -VE -VE -VE +VE +VE -VE -VE -VE +VE +VE -VE -VE -VE +VE +VE +VE +VE -VE
1% +VE 2.5% +VE 5%
7.5% -VE 10%
Colony morphology for identification of micro organisms
1. S.aureus form large colonies of 2-4 mm dia,yellowish Circular clusters • E.coli form large ,thick grayish colonies.
3. P.mirabilis show swarming motility& negative to lactose. 9. C.albicans are yeast like fungus . 5. 6. Agar diffusion method-None of dilution show zone of inhi bition for any organisms. Agar dilution method-A: Shweta parpati ,B: Sheetal parpati Growth indicated by +VE No growth indicated by -VE
1.The pH of both parpatis fall in range that is required for bacterial growth. So it can be said that action of parpatis are independent of change in pH. 2.The two parpatis show encouraging antimicrobial activities at different concentrations in different strains of bacteria. 3. Antibiotic susceptibility tests are not guarantee that an Anti microbial activity will be effective or ineffective 4.The general method of diagnosis in ayurveda is based on doshas like vata ,pitta and kapha.So ayurvedic formulations have different role in every human being. 5.This study gives a scope for in vivo studies regarding action of these formulations in reducing puss and albumin.