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Practical Physiology

6th. Lab

.Sedimentation Rate
.Bleeding time
.Clotting time
Lab. no. 6: Erythrocyte Sedimentation Rate
(Westergren method)
Under aseptic conditions withdraw (1.6 ml ) of blood
from a vein and mix with (0.4 ml) of 3.8% sodium
citrate as anticoagulant (4:1), suck this blood into a
Westergren tube, adjust the top level at (0 mm)
mark. Wipe the blood off its tip and set the tube in a
vertical position in the Westergren rack. RBCs settle
down leaving a column of clear plasma on the top.
After exact one hour read the upper level of the RBC
column. The result is expressed in mm/1st hr.
Lab. no. 6: (ESR) (Westergren method) Cont…..

-Normal ranges of ESR:

Male 0 –15 mm/1st hr.
Female 0 –20 mm/1st hr.
ESR is i ncre ased p hysiolo gic ally
1. Menstrual period.
2. Pregnancy.
ES R has non-specific diagnostic value; that is a
rise in ESR indicates the presence of some
organic disease.
ES R estimation also has a prognostic value and
a fall in ESR after treatment indicates good
Lab. no. 6: Bleeding time (Dukeś method)

• Clean a finger ball with spirit and with a
sterile lancet give at least 2 mm deep
prick. Note the time.
• Every 30 sec. blot off the drop of blood
by a filter paper until no more blood
appears on the puncture site. Note the
time at which the bleeding has stopped.
Lab. no. 6: Bleeding time (illustration) Cont…..

.Every 30 sec

Bleeding time test

(Duke’s method)

no. of blots
B.T = = ? min.

-Normal bleeding time (B.T) = 3 – 6 min.
Lab. no. 6: Bleeding time (Dukeś method) Cont…..

Estimation of bleeding time is not a reliable test,

because the results of this test would vary
depending on the site and depth of the puncture.
Moreover it is shortened in cold weather, due to
capillary constriction, and reverse is the case in
hot weather.
-It is especially prolonged by lack of platelets
(Thrombocytopaenia ).
Events in haemostasis (Arrest of bleeding):
1- Vasoconstriction.
2- Platelet aggregation.
3- Formation of platelet plug.
4- Blood coagulation. Clotting time (capillary tube method)

6.Clean the finger with spirit and give a deep prick
with a sterile lancet, squeeze the finger to obtain
a large drop of blood. Note the time (T1).
7.Fill in two plain capillary tubes with blood.
8.After (2 min.), start breaking small pieces of one
capillary tube every 30 sec. until a fibrin thread
is seen in the interval between the 2 broken
equivocal ends. Note the time (T2).
9.The interval between T1 and T2 is the clotting
time. Clotting time (illustration)

Fibrin thread

1 2 3 4 5 6 7 8

.(Clotting time test (capillary tube method

.Normal Clotting time (C.T) = 5 – 8 min-

.It is increased in haemophilia-