Fungi-like bacteria

Actinomyces species Nocardia species Streptomyces species
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Actinomycetes

Aerobic gram-positive bacilli
   

Nocardia Actinomadura Nocardiopsis Streptomyces

Anaerobic non-spore forming gram-positive bacilli

Actinomyces

Gram Positive Filamentous Bacteria
GENUS
Mycobacteria Actinomyces Nocardia

OXYGEN aerobe
ANAEROBE

GRANULE ACIDFAST no YES sometime YES yes no
PARTIALLY

aerobe aerobe

Streptomyces

no

Physiology

Bacteria with fungi-like structures. Actinomycetes are true bacteria

lack of nucleus membrane and mitochondria susceptibility to antibacterial but not to antifungal

Acid-fast stain of Nocardia sp.

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Physiology
 There are aerobic and anaerobic

actinomycetes

Aerobe; Nocardia, Actinomadura, Streptomyces Anaerobe; Actinomyces 3 days - 2 weeks

 Slowly growing

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Nocardia growing in culture after 7 days as colony that are c halky and raised,with color varyi ng from white to yellow-orange

Diseases
Actinomycetoma Nocardiosis (Nocardial infection) Actinomycosis

Modified acid-fast stain

Acid-fast stain
 

Modified acid-fast stain
  

carbolfuchsin 3%HCl-alcohol Methylene blue

carbofuchsin 1% H2SO4- alcohol Methylene blue

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Actinomycosis
 

Lumpy jaw Chronic disease characterized by formation of multiple abscesses and sinus tracts that discharge sulfur granules

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Actinomycosis

Etiologic agent: Anaerobic bacteria
 

Actinomyces israeli, A. gerencseriae A. naeslundii, A.odontolyticus, A. viscosus

Gram-positive branching filamentous, no spore forming Acid-fast negative www.ams.cmu.ac.th

Actinomycosis

Source : Normal flora of the upper respiratory, gastrointestinal and female genital tracts 3 major clinical types
  

Cervicofacial actinomycosis (50-70%) Thoracic actinomycosis (15-20%) Abdominal & pelvic actinomycosis (10-20%)

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Actinomycosis

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Actinomycosis

Laboratory diagnosis

Specimen : pus, sputum, skin biopsy Naked eye ; sulfur granule 10 % KOH, Gram’s stain

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Sulphur granules

Actinomycosis

A) Microscope view of actinomycotic “sulfur granules” (10x) (Exudate from an abdominal wound infection)

B) Gram-stained smear from the specimen as in A), showing thin sulfur granule with thin, branching filaments (800x)

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Actinomycosis

Laboratory diagnosis

Cultures immediately under anaerobic conditions and incubated for 48 hours or require 2-3 weeks. Typical colonies have "molar tooth" appearance on agar

Molar tooth colony

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Actinomycosis

Treatment

1 to 2 months of penicillin given through a vein, followed by 6 to 12 months of penicillin taken by mout h. co-trimoxasole, chloramphenicol, methicilin

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