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Baseline susceptibility and stability of insecticide resistance in Spodoptera litura (F.

)
Adeel Rehan, Mushtaq A. Saleem, Sarfraz A. Shad, Ghulam M. Cheema and Umair Faheem

University College of Agriculture, B. Z. University, Multan

groundnut. and vegetables such as cucurbits. maize. berseem. and brassica • May cause 26–100% yield loss in the field • High resistance against conventional insecticides • Low to medium resistance against new chemistry insecticides . sweet potato. brinjal. potato.Introduction • Polyphagous defoliating insect pest found in Asia and Oceania • Important crops which are attacked in Pakistan are cotton. summer legumes. tobacco.

Life stages of Spodoptera litura Larva Eggs Moths Pupae .

litura against four different insecticides i. imidacloprid and profenofos • To observe the susceptibility of succeeding generations of S. spinosad. emamectin benzoate. litura to different insecticides under laboratory conditions • To check the stability of insecticide resistance in S. litura .Main Objectives • To generate the baseline susceptibility data of S.e.

Materials and Methods • Larvae of S. litura were collected from dunyapur • The larvae were reared on improved artificial diet at 27C°±2 temperature and 70-80% humidity Toxicological studies • Insecticides Spinosad emamectin benzoate profenofos imidacloprid • Bioassay method Diet incorporation method • Data analysis .

77 LC50 value(ppm) 4.53 1.Results 0 1 2 3 4 5 6 7 8 9 10 7.72 3.14 0.57 4.95 2.66 1.79 1 4 5 7 8 9 10 11 Generations Toxicity of spinosad against Spodoptera litura under laboratory conditions .

19 1.33 0 1 1 4 5 7 8 9 10 11 Generations Toxicity of emamectin benzoate against Spodoptera litura under laboratory conditions .5 LC50 value(ppm) 6 3.13 0.85 2 3 4 1.7 0.14 1.37 1.4 0.

8 50 40.300 LC50 value(ppm) 200 250 258.61 18.75 100 150 136.5 52.07 49.62 19.71 0 1 4 5 7 8 9 10 11 Generations Toxicity of profenofos against Spodoptera litura under laboratory conditions .71 24.

6 131.14 73.8 96.5 LC50 value(ppm) 359.7 134.3 314.5 0 1 4 5 7 8 9 10 11 Generations Toxicity of imidacloprid against Spodoptera litura under laboratory conditions .100 200 300 400 500 600 700 800 686.1 75.

7 profenofos 258.34 9.11 10.77 3.41 imidacloprid spinosad emamectin benzoate 73.36 11.71 13.104 9.85 Final LC50 (ppm) RF Resistance Decline R -0.5 7.088 -0.Insecticides Susceptibility Index Initial LC50 (ppm) 686.61 .33 9.79 0.83 11.75 18.8 -0.090 -0.5 0.096 G 11.

litura  S. litura has resistance against non-target insecticide (imidacloprid) that may cause cross resistance problems.Conclusion  This baseline susceptibility data can be used for future insecticide monitoring of S. .