The Antiglobulin test

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The addition of AHG containing anti-IgG to RBCs sensitized with IgG antibodies allows for hemagglutination of these sensitized cells. IgM antibodies bind to corresponding antigen and directly agglutinate RBCs suspended in saline. IgG antibodies are termed nonagglutinating because their monomer structure is too small to agglutinate sensitized RBCs directly. . Some blood group antibodies have the ability to bind complement to the RBC membrane. IgM and IgG.There are two major types of blood group antibodies. Antiglobulin tests detect IgG and/or complement-sensitized RBCs. Because of their large pentamer structure.

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2. Antibody molecules and complement components are globulins. . including antiIgG. AHG reacts with human globulin molecules. Injecting an animal with human globulin stimulates the animal to produce antibody to the foreign protein (i. either bound to RBCs or free in serum. 3. AHG).and polyspecific reagents that contain both anti-IgG and anti-C3d activity..e. Washed RBCs coated with human globulin are agglutinated by AHG.1. 4. Serologic tests employ a variety of AHG reagents reactive with various human globulins. antibody to the C3d component of human complement.

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