Professional Documents
Culture Documents
Composition of blood-
Whole blood
55% plasma
10% solutes
90% water
45% cells
red
white
platelets
nutrients
agranulocytes
granulocytes
salts (electrolytes)
eosinophils
hormones
monocytes
lymphocytes
basophils
plasma proteins
T cells
albumens
globulins (antibodies)
clotting factors
killer T
cells
helper
T cells
B cells
neutrophils
Blood cells
Platelets
Neutrophil
Monocyte
T-lymphocyte
False colour scanning electron micrograph of cells
in arterial blood
Objectives:
* describe the role of haemoglobin in carrying oxygen and carbon dioxide
** describe and explain the significance of the dissociation curves of adult
oxyhaemoglobin at different carbon dioxide levels (the Bohr effect)
:
*
**
( )
- -,
a-globin
b-globin
haem
Each haem group can bind one oxygen molecule (O2) to its iron atom: one haemoglobin
molecule can therefore carry four oxygen molecules
Unbound haemoglobin has quite a low affinity for oxygen, and at low oxygen concentrations
(as in respiring tissues) very little is bound
But the binding of an oxygen molecule to one haem group alters the shape of the whole
molecule, making it easier for a second to bind; this alters the shape making it easier for a
third to bind, and so on: this gives the characteristic oxygen dissociation curve of
haemoglobin
(O2)
:
,
(, )
, ;
:
Percentage
saturation of
haemoglobin
with oxygen
50
.
, ""
: ,
= 0%, , = 100%,
, , = 50%, .
0
Partial pressure of oxygen (pO2) / kPa
(2) /
Percentage
saturation of
haemoglobin with
oxygen
50
, ,
, ,
.
(,%, 3,
) .
0
Partial pressure of oxygen (pO2) / kPa
(2) /
Partial pressure of a gas in a mixture of gases is the part of the total pressure
of the mixture that is exerted by that gas alone.
E.g. if total atmospheric pressure is 100 kPa, and oxygen makes up 20.7% of
the atmosphere, than the pO2 in the atmosphere is 20.7kPa.
Percentage
saturation of
haemoglobin with
oxygen
50
, , .
100
20,7% , 2 20.7kPa.
2000
40% , 2 ?
800 kPa
0
Partial pressure of oxygen (pO2) / kPa
(2) /
Percentage
saturation of
haemoglobin 50
with oxygen
12.5 kPa
Partial pressure of oxygen (pO2) / kPa
Alveolar pO2
100
Percentage
saturation of
haemoglobin 50
with oxygen
0
0
but
binds, the curve,
is not
linear
,
increases in gradient
.
2
4
6
8 10
12
14
Partial pressure of oxygen (pO2) / kPa
Alveolar pO2
100
Percentage
saturation of
haemoglobin 50
with oxygen
Compare the oxygen
dissociation curve to the curve
we would see if haemoglobin
reached saturation at the same
pO2 but without the increasing
affinity.
,
pO2
.
pO2 of exercising
muscle
0
0
2
4
6
8 10
12
14
Partial pressure of oxygen (pO2) / kPa
Alveolar pO2
At the placenta, fetal haemoglobin must load
(take up oxygen) at a pO2 at which maternal
haemoglobin unloads.
100
Percentage
Fetal
saturation of
haemoglobin
haemoglobin 50
with oxygen
Maternal
haemoglobin
,
( )
2,
.
,
, :
.
0
0
2
4
6
8 10
12
14
Partial pressure of oxygen (pO2) / kPa
Alveolar pO2
100
Myoglobin
Percentage
saturation of
haemoglobin 50
with oxygen
pO2 of exercising
muscle
Adult
haemoglobin
0
0
2
4
6
8 10
12
14
Partial pressure of oxygen (pO2) / kPa
5%
2,
25%
carbaminohaemoglobin
The remaining 70% enters red blood cells and is converted by the enzyme
carbonic anhydrase into carbonic acid
The carbonic acid immediately dissociates into hydrogen ions and
hydrogencarbonate ions:
70%
:
CO2 + H2O
H2CO3
H+ + HCO3-
The hydrogencarbonate ions diffuse out into the plasma: the loss of negative ions is
balanced by a corresponding uptake of chloride ions, Cl- (the chloride shift)
:
, Cl-(
)
The diagram represents a red blood cell with oxygen-saturated haemoglobin, arriving at a respiring
tissue with a high CO2 concentration
,
CO2
CO2
CO2 + H2O
Carbonic
anhydrase
H2CO3
H+
HCO3-
O2
O2 H + O 2
O2
Haemoglobinic
acid
CO2
CO2 + H2O
Carbonic
anhydrase
H2CO3
In the alveolar capillaries the opposite
happens: the high O2 concentration
displaces H+ from haemoglobin; the H+
ions combine with HCO3- to form
carbonic acid, which decomposes to
CO2 and water.
H+
HCO3-
4O2
H+
Haemoglobinic
acid
haemoglobinic ,
2
( ): .
100
Percentage
saturation of
haemoglobin 50
with oxygen
Low CO2
level
High CO2
level
Medium CO2
level
Unloading
occurs here
0
0
2
4
6
8 10
12
14
Partial pressure of oxygen (pO2) / kPa
% saturation
% saturation
% saturation
Llama
haemoglobin
Human
haemoglobin
,
,
pO2 ,
.
?
.
% saturation
Fetal
haemoglobin
at pCO2 0.1
kPa
Adult
haemoglobin
at pCO2 0.2
kPa
()
2 0,1 , ()
2
0,2 ? .