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Enzyme - 1 Classification and Kinetics

Harliansyah, PhD Head Dept. of Biochemistry Faculty of edicine, !"#$% &ni'ersity

()*ecti'es
+ Descri)e the chemical properties of enzymes, e-. "cti'e site, catalytic efficiency, specificity, cofactor, re-ulation, compartmentalisation. + E.plain the classification of enzymes + Descri)e the /inetics of enzyme catalysis, -. ichaelis enten e0uation -. 1ine2ea'er Bur/ transformation #eferences, 1. #ao, 3. . 4556. edical Biochemistry. 4nd Ed. 3e2 "-e, Pu)lisher. 4. Baynes, 7.8. Dominicza/, .H. 4559. edical Biochemistry. 4nd Ed. Else'ier os)y.

or-an. cellular. Catalytic action can )e re-ulated < to ensure rate of product formed not in e. .the e0uili)rium Enzymes different from other catalyst. tissue.cess of amount needed 1ocalization. $pecific . -. each enzyme eat different reaction -.8hat is Enzyme : + + + Catalyst %ncrease rate of chemical reactions 2ithout chan-in.reater catalytic po2er than other catalyst -. su)cellular Holoenzyme = "poenzyme > Cofactor #eactant = $u)strate Classified in to 6 -roups = type of reaction + + + + .

and surface -eometry 2hose specificity depends on their tertiary structure. Ahe tertiary structure determines the three-dimensional shape. function. Ahey are each responsi)le for control of a sin-le reaction and are thus responsi)le for control of meta)olism.+ Enzymes are proteins -lo)ular ?ri)ozyme is catalytic acti'e of #3"@ each 2ith a specific structure ?nati'e conformation@. distri)ution of electrical char-es. .

1."cti'e $ite = three dimensional cleft created )y side chain of se'eral amino acid "mino acid from different re-ions in the linear se0uence of enzyme. Bindin. Catalytic residues . side chains close at the acti'e side "mino acid is classified into 4 functional -roups.residues 4. folded.

.In an enzyme.4 types of binding of small molecule (red) to the binding site of a protein (shaded) Electrostatic Hydrophobic Hydrogen Bond Van Der Walls . a `binding site’ = an `active site’ .

$u)strate + 4.roup + C. . #eaction + B.Enzyme $pecificity + 1. ")solute -roup .

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Effect enzymes on acti'ation ener-y .

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#eaction Profile .

E.amples of #eaction Profile .

E.amples of #eaction Profile .

1. Concentration .Factors That Influence Reaction Rate &nder a specific set of conditions. 4.the reaction.molecules must mi.molecules must collide to reactD Physical state .molecules must collide 2ith enou-h ener-y to reactD Ahe use of a catalyst. Four factors can )e controlled durin. C. to collideD Aemperature . B. 2hich depends upon the chemical nature of the reactants. e'ery reaction has its o2n characteristic rate. .

Cascade Model : Type reaction of enzymes .

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Effects of temperature Each enzyme has an optimal temperature in 2hich it can function .

Effects of pH Each enzyme has an optimal pH in 2hich it can function .