Enzyme - 1 Classification and Kinetics

Harliansyah, PhD Head Dept. of Biochemistry Faculty of edicine, !"#$% &ni'ersity

()*ecti'es
+ Descri)e the chemical properties of enzymes, e-. "cti'e site, catalytic efficiency, specificity, cofactor, re-ulation, compartmentalisation. + E.plain the classification of enzymes + Descri)e the /inetics of enzyme catalysis, -. ichaelis enten e0uation -. 1ine2ea'er Bur/ transformation #eferences, 1. #ao, 3. . 4556. edical Biochemistry. 4nd Ed. 3e2 "-e, Pu)lisher. 4. Baynes, 7.8. Dominicza/, .H. 4559. edical Biochemistry. 4nd Ed. Else'ier os)y.

each enzyme eat different reaction -. or-an. su)cellular Holoenzyme = "poenzyme > Cofactor #eactant = $u)strate Classified in to 6 -roups = type of reaction + + + + . cellular.8hat is Enzyme : + + + Catalyst %ncrease rate of chemical reactions 2ithout chan-in. .reater catalytic po2er than other catalyst -. tissue. Catalytic action can )e re-ulated < to ensure rate of product formed not in e. $pecific .the e0uili)rium Enzymes different from other catalyst.cess of amount needed 1ocalization. -.

. Ahe tertiary structure determines the three-dimensional shape.+ Enzymes are proteins -lo)ular ?ri)ozyme is catalytic acti'e of #3"@ each 2ith a specific structure ?nati'e conformation@. distri)ution of electrical char-es. and surface -eometry 2hose specificity depends on their tertiary structure. Ahey are each responsi)le for control of a sin-le reaction and are thus responsi)le for control of meta)olism. function.

"cti'e $ite = three dimensional cleft created )y side chain of se'eral amino acid "mino acid from different re-ions in the linear se0uence of enzyme. Catalytic residues . 1. folded. side chains close at the acti'e side "mino acid is classified into 4 functional -roups. Bindin.residues 4.

In an enzyme.. a `binding site’ = an `active site’ .4 types of binding of small molecule (red) to the binding site of a protein (shaded) Electrostatic Hydrophobic Hydrogen Bond Van Der Walls .

Enzyme $pecificity + 1. #eaction + B. . ")solute -roup . $u)strate + 4.roup + C.

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Effect enzymes on acti'ation ener-y .

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#eaction Profile .

E.amples of #eaction Profile .

E.amples of #eaction Profile .

4. 2hich depends upon the chemical nature of the reactants.molecules must mi. e'ery reaction has its o2n characteristic rate.molecules must collide to reactD Physical state .the reaction.Factors That Influence Reaction Rate &nder a specific set of conditions.molecules must collide 2ith enou-h ener-y to reactD Ahe use of a catalyst. C. B. . Concentration . 1. Four factors can )e controlled durin. to collideD Aemperature .

Cascade Model : Type reaction of enzymes .

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Effects of temperature Each enzyme has an optimal temperature in 2hich it can function .

Effects of pH Each enzyme has an optimal pH in 2hich it can function .