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BHOONAGA SATVA BHASMA :

A POTENT AND LESS TOXIC


ALTERNATIVE TO TAMRA BHASMA

Guided By: Presented By:


DR.R.S.HIREMATH MD DR. VIKAS
SAROCH
Asst. Professor II P.G. Scholar
Dr. (Mrs.) P.P.Dindore M.D.
Dr. Manojkumar S.Patil M.D.

P. G. Department of Rasashastra
K L E U’s
Shri. B.M.K.Ayurved Mahavidyalaya,
INTRODUCTION
• Mineral origin of material contains many physical ,
chemical impurities.
• Purification (Shodhana) and reduction to bhasma
(Marana) form, is lengthy, tedious procedure in
some cases.
• In Rasagranthas we have reference about
Bhunagasatwabhasma.
• Explained as substitute to Tamrabhasma which is
of animal origin ( which requires comparatively less
duration).

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02,2009 TOXIC ALTERNATIVE TO TAMRA BHASMA
EXPERIMENTAL STUDY

(Acute toxicity Guideline No.423 ANNEX 2d)

• DIVIDED IN TWO PHASES

• LD- 50 dose estimation of Bhunaga SatwaBhasma


and Tamra Bhasma.

• Comparitive Toxicity study of Bhunaga SatwaBhasma


& Tamra Bhasma(Market Sample).

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BHASMA PARIKSHA
• Bhunagasatwa Bhasma passed all Bhasma
Pariksha including Amla, Nirutha, Apunarbhava
Pariksha.
• Selection of Tamra Bhasma from market Sample
A, B & C those Bhasma pariksha were carried
out.
• Sample C passed all Bhasma Pariksha
including Amla pariksha. This sample was taken
for Toxicity study.

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02,2009 TOXIC ALTERNATIVE TO TAMRA BHASMA
TOXICITY STUDY
• No standard parameters for dose administration of
Bhunagasatwa Bhasma.

• LD 50 estimation of Bhunagasatwa Bhasma & Tamra Bhasma.

• Acute toxicity for 14 days.

• As per OECD guidelines of CPCSEA forum.

• Tamra Bhasma group shows mortality 2 animals out of 3


and Bhunagasatwa Bhasma no mortality.

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02,2009 TOXIC ALTERNATIVE TO TAMRA BHASMA
LD50 Dose Estimation of Bhunaga
SatwaBhasma
• Bhunaga SatwaBhasma 2000 mg/kg
No Animal Dose(ml) Observation of toxicity reactions
weight(g) Onset of time Recovery Toxicity
period signs

1 24 0.46
-- -- N

2 22 0.44
-- -- N

3 22 0.44
-- -- N

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02,2009 TOXIC ALTERNATIVE TO TAMRA BHASMA
LD50 dose estimation of Tamra Bhasma (Market Sample)
Animal no Dose Onset of Recover Toxicity signs observed
& weight mg/kg time y period 30 min 1hr 2hr 3hr 4hr 24hr
(g)

1 24 2000 15 min 4hrs c, rd, n rd, n rd, n rd, n rd, n N


2* 22 2000 30 min -- c, rd, n c, rd, n, t -- -- -- --
3* 22 2000 30 min -- c, rd, n c, rd, n c,rd,n,s,d c,rd,n,s,d c,rd,n,s,d --

1* 30 1000 30 min -- rd, n c, rd, n c, rd,n,s -- -- --


2 28 1000 40 min 2hrs n n N N N N
3* 25 1000 30 min -- n, rd n n, s n, s n, s --

1 30 500 N N N N N N
2 28 500 N N N N N N
3 25 500 N N N N N N
1 30 300 N N N N N N
2 26 300 N N N N N N
3 25 300 N N N N N N

*animalsdied during study; c-catatonia; d-diarrhea; n-narcosis; N-normal; rd-respiratory


depression; s-salivation BHOONAGA SATVA BHASMA:A POTENT AND LESS
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Acute oral toxicity record sheet
Species: Mice (Swiss albino) Sex: Female Observation time: 24 hrs ,Duration: Upto 14 days
Dose: Test drug (BSB) – 200mg/kg
Standard drug (TB) – 100 mg/kg

Group Animal no Dose Onset of Recovery Toxicity signs observed


& weight ml/kg time period
(g) 30 1 2 3 4 24 6th 7th 14th
min hr hr hr hr hr day day day

Control 1 25 water N N N N N N N N N N N
(q.s.)
2 28
3 30
Bhunaga 1 28 0.56 N N N N N N N N N N N
Satwa
Bhasma 2 36 0.70 N N N N N N N N N N N
3 26 0.52 N N N N N N N N N N N

Tamra 1 30 0.15 N N N N N N N N N N N
Bhasma
(market 2 26 0.13 N N N N N N N N N N N
sample)
3 25 0.12 N N N N N N N N N N N
Quantitative analysis of and Bhunagasatwa
Bhasma & Tamra Bhasma
Sl.no Test BhunagasatwaBhasma TamraBhasma

1 Copper 11.35% 63.84%

2 Borax 68.88% --

3 Sodium 10. 94 % --

4 Sulphur Nil 2.76%

5 Mercury -- ---

6 Iron -- 5.34%

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ACUTE ORAL TOXICITY

• LD 50 Determination of Tamra Bhasma was done on 1000


mg/kg b.w. where as for Bhunagasatwa Bhasma it is safe at
2000mg/kg (higher dose) and can be considered as a safe
dose.

• Hematology & Biochemical parameters -


The Hb % of both groups are increased.

• S.G.P.T. S.G.O.T. & serum Alkaline phosphate in Tamra


Bhasma group was more increased compared to that of
Bhunagasatwa Bhasma group.
It shows some obstructive pathology causing liver damage.

• Histopathology –
HPR shows mild congestion in Bhunagasatwa Bhasma where
as in Tamra Bhasma shows moderate congestion.

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CONCLUSION
• LD50 Dose estimation of Tamra Bhasma was estimated
at 1000mg/kg and Bhunagasatwa Bhasma was safe at
higher dose 2000mg/kg.

• Tamra Bhasma shows more significant Hepato toxicity


where as Bhunagasatwa Bhasma shows minimum
toxicity.

• Bhunagasatwa Bhasma can be used as substitute for


Tamra Bhasma.

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REFERENCES
• “Preparation and Physico-chemical analysis of Bhunagasatwa Bhasma and its
comparative toxicity study with Tamra Bhasma (Market Sample)” By  Dr.
Manojkumar S.Patil

• Ayurved Prakasha Edition-1999,Pp:419

• Ayurved Prakasha,Edition-1999,Pp:418.

• Rasatarangini,11th Edition- 1979,Pp : 433.

• Fundamentals of Experimental Pharmacology, Scientific Book Agency,


Calcutta, 2nd Edition, Pp :156

• Committee for the Purpose of Control and Supervision of Experiments


on Animals (CPCSEA ), Govt. of India,(Rev.)- 2000.

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