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Baldovino, Andre

Bayle, Sophia
Bariata, Don Kim
Briones, Karen
Bungay, Elka
B1 Group 3
Received: 16 November 2013; Received in
revised form: 21 January 2014; Accepted:
16 February 2014
Conducted a study to compare the dietary intake of
essential fatty acids (FAs) and serum levels of
inflammatory factors in both asthmatic and healthy
adults
It aimed to examine the correlation between
inflammatory markers and FAs.
Performed using a CASE-CONTROL study on:
47 ASTHMATIC patients (26 males and 21 females) VERSUS
47 CONTROLS (24 males and 23 males)
Tests performed on participants:
TNF-alpha, high sensitive C-reactive protein (hs-CRP), leptin and
adinopectin
Assessment by semi-quantitative food frequency questionaire
(FFQ)


Results:
Asthma Controls
Dietary intake of
omega-3 FAs
Significantly
LOWER
-
Serum
Concentration
(TNF-alpha, hs-
CRP, leptin)
Significantly
HIGHER
-
Dietary intake of
adinopectin and
saturated FAs
negative negative
Correlation
between other
inflammatory
factors and
dietary intakes
NOT significant NOT significant
Higher intake of
omega-3 and lower
levels of
inflammatory
factors in the
healthy control
group compared to
asthmatic group
may explain the
protective role of
essential fatty acids
in asthma.
General Objective
To compare the dietary intake of essential fatty
acids (FAs) and serum levels of inflammatory
factors in both asthmatic and healthy adults

Specific Objectives
To examine potential relationship between
inflammatory markers and dietary fatty acids.
To measure serum concentration TNF-alpha , hs-CRP ,
leptin and adiponectin of both cases and controls.
To statistically analyze the data by independent t-test and
ANCOVA.


SUBJECT
CASES:
47 diagnosed with asthma
-26 males,21 females
CONTROL:
47 HEALTHY ADULTS
-24 males,23 females
Random sampling from patients list that
satisfied criteria of the study
Classified asthma severity
Categories:
a.symptoms
b.lung function
c.activity restrictions
Subjects were informed of the
researchers objectives
Written consent was obtained
Identified according to
American Thoracic Society
definition

Anthropometric measurement were taken
aWeight
b height,
cwaist circumference
dhip circumference
ewaist to hip ratio
fBMI
gBody adiposity index
(hip circumference/height
Assessment of dietary intakes
Semi-quantitative food frequency
questionnaire (FFQ)
Nutritionist IV program was used to estimate
dietary intakes
BIOCHEMICAL ANALYSIS
STATISTICAL ANALYSIS
RESULTS
Fasting venous blood sample
All data were presented
As mean SD
Independent sample t test
-comparison of absolute intake of fatty
acids and levels of inflammatory factors
Kolmogorov-Smirnov test
-normal distribution of all
variables
ANCOVA
-covariates
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Presented in Table 3, there is a significant
difference between the two groups on the
serum levels of inflammatory biomarkers.
Since, serum concentrations of TNF-, hs-
CRP, and leptin were significantly higher in
the asthmatic group than in the control
group, this denotes an increased
inflammatory factor, owing to its pro-
inflammatory property, in asthmatic patients
Thus, values significantly differ when
compared to a healthy participant.



As seen in Table 5, Adinocpectin level of both asthmatic
and control groups presents a positive correlation to
Omega 3 fatty acids and its derivatives mainly EPA,
DHA, and ALA. Thus, the high concentration of Omega
3 Fatty acid and its derivatives may inhibit the action of
Adinopectin.
Leptin level also showed a positive correlation with EPA
and DHA on both asthmatic and controlled groups but
only showed positive correlation for Omega 3 Fatty
acid (gm). While, ALA on the asthmatic group had a
negative correlation.
Omega 3 Fatty acid also showed a positive correlation
between tNF- alpha and hs CRP of Asthmatic group,
hence only making it inconclusive.


Higher intake of -3 fatty acids and lower
levels of inflammatory factors in the healthy
control group compared to asthmatic group
explains the protective role of essential fatty
acids in in asthma.
However, no significant correlation between
-3 and -6 intakes and serum levels of
inflammatory factors was found in this study.
Therefore, subsequent studies with larger
sample size are needed in this regard.