EXPERIMENT RESULT: WORK OF CATALASE ENZYME
COMPILED BY GROUP III XII-A8: • Albertus Erick B • Kurnia Susvitasari • Martha Hindriyani • Nikko Adhitama
• • • • •
To observe the work of catalase enzyme in various substrate and various pH
Catalase is an enzyme found in nearly every cells Produced by an organell named Peroxixome. Convert hydrogen peroxide to water and oxygen
H2O2 → H2O + O2
Enzyme Works on Specific pH Therefore, catalase enzyme do work in specific pH as well.
optimum pH for catalase enzyme is 7 (neutral)
Catalase Mostly Found Only in Specific Cells catalase enzyme is produced by an organelle named Peroxixome. Peroxixome mostly found in liver cells.
concentration in liver cell
∴ Catalase will have bigger
Tools and Materials
Reaction tube Ruler (1 pc) Substrate:
liver of Gallus gallus (chicken) Crushed Carrot Crushed Potato Hydrochloride Acid (HCL) Sodium Hydroxide (NaOH) Hydrogen Peroxide (H2O2)
Plastics Ribs Rubber
(9 pcs) (± 10 cm) (10 pcs)
Prepare all materials and tools Put crushed liver, crushed potato, and crushed carrot in each three reaction tube. Name the tube for each substrate with A, B, and N. Give 10 drops of HCL in tube A, 10 drops of NaOH in tube B for each substrate. Let tube N untouched. Give 10 drops of H2O2 in all tubes. Quickly wrap
the tube with plastic and bundle it with rubber Wait for about 300 seconds (5 minutes).
After 5 minutes, open the plastic wrap and do “Gas Test” by using ember ribs for all tubes. Pay attention to the ember of the ribs. Note the result
No 1 2 3 Substrate Liver Carrot Potato pH Acid Small ember, little foam no No ember, foam No foam, no ember Bases Neutral Light ember, Very light and little foam little long lasting No ember, Light long foam lasting ember, No ember, very ember,ember, Small much foam foam much foam little foam little
Catalase enzyme work best in neutral condition. This is proven by the lighter ember in neutral-substrate during Gas Test. Catalase enzyme have higher concentration in liver cell. This is proven by the lighter ember in chicken liver than the other substrate. Catalase enzyme do react with hydrogen peroxide forming O2 gas. This is proven by the lighter ember during gas test.