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Theoretical Principles and Practical Skills of

Aseptic Manipulations

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Definitions Background Laminar Air Flow Hoods Aseptic Technique Free Powerpoint Templates Page 2 .

by mouth. orally. per tube) • Parenteral Page 3 .Definitions • Enteral – Of or relating to the intestines (e.g.

g.Definitions (cont. microorganism contamination) Page 4 .) • Contamination – any effect or action that has a negative impact on a product's integrity making it unfit for use – Chemical composition – pH – Sterility (e.

Background Sources of product contamination • People (most common) – Touch contamination – Generation of particulates from shedding cells or hair Page 5 • Supply air .

Laminar Air Flow Hoods • The underlying principle of a laminar air flow hood is that a constant flow of HEPA filtered air at a rate of approximately 90 linear feet per minute physically sweeps the work area and prevents the entry of contaminated air • The hood workspace is used to prevent the contamination of compounded sterile products. Page 6 .

Laminar Air Flow Hoods (cont.) HEPA Filter in Frame Page 7 .

Laminar Air Flow Hoods (cont.) • Horizontal Flow (Laminar Flow Hood) – Air blows towards worker Page 8 .

) • The HEPA filter is located in the fragile mesh between thin metal strips at the back of the hood behind the HEPA filter screen • Nothing should be permitted to come in contact with the HEPA filter – NO cleaning solution Page 9 .Laminar Air Flow Hoods (cont.

Laminar Air Flow Hoods (cont. or Labels in the Hood • Only products essential to product preparation should be placed in the laminar flow hood to minimize the potential for contamination Page 10 . Pens. Calculators.) No Paper.

Laminar Air Flow Hoods (cont.) • Eating. drinking. and smoking is always prohibited • Talking or coughing should be directed away from the hood to minimize air flow turbulence • A mask covering mouth and nose must be worn Page 11 .

Autoclaves Safe and Effective Use Page 12 .

Principles of Steam Sterilization (cont.) The “killing power” of steam is due to its latent heat of vaporization – 80 calories: 1 L water to boilingg – 540 calories: 1 L boiling water to steam Page 13 .

Action of Steam Steam contacts a cooler surface. condenses. causing a huge decrease in volume and setting up a negative pressure that draws more steam Condensation occurs as longg as there is a temperature differential Page 14 .

Standard Temperature and Pressure 250 °F (121 °C) and 15 p.s. Berapa atm ? .i.

inverted 15 Instruments. wrapped 30 Page 16 .How Long to Autoclave Dry goods @ 121 °C Time (min) Glassware. empty.

Special Situations Media boils out of bottles – differs among media Very dense or closed dry objects Page 17 .

TERIMA KASIH Page 18 .