Laboratory Design and

Chapter 4

Planning of tissue culture labs
Importance of asepsis
Old labs – modifying ventilation and air-
conditioning, structural modifications will be
New labs – Integrative and innovative design
- Facilities may be positioned for ergonomic and
energy-saving reasons

Laminar Flow Hoods – Positions of air inlets and extracts . Positive pressure – Avoid influx of contaminated air into surroundings of lab . Negative pressure – Usage of human material . Planning of tissue culture labs Ventilation – Pressure balance and Laminar Flow Hoods . Positive-pressure buffer zone – Preparation area and microscope .

12 laminar hoods/50 people .How many people will work each week? .Area required to handle bioreactors .What kinds of culture will they perform? . Planning of tissue culture labs Accommodation .

microscopes.Area for wash up.Largest area – culture operation accommodating laminar-flow hoods. cell counters. Planning of tissue culture labs Space – . . centrifuges. stocks of reagents.Area for storage . glassware and plastics etc. incubators. and arrangements for media. preparation and sterilization .Area for incubation .

UV influx and incursion of microorganisms . Planning of tissue culture labs Aseptic area .Windows – high heat generation.Animal facility is accessible but not contiguous to tissue culture labs .

Minimize interference in airflow between hoods . Planning of tissue culture labs Hoods – . Space filled with removable cart or trolley . Space between hoods should be 500 mm (2 ft) . Motion movement .

Large number of flasks or large volume flasks (sealed) incubated in hot rooms .Open plates and dishes incubated in humid Co2 incubator . Planning of tissue culture labs Incubation – .

ii) outside wall to allow for heat extractions from ovens and steam vents from autoclaves .Facilities for washing up and for sterilization should be located .i) close to aseptic area that they service . Planning of tissue culture labs Preparation area – .

Maximum load capacity of ramp without any mechanical help Storage – .Cell line work with liquid nitrogen . Planning of tissue culture labs Servicing aseptic areas – .Space required for disposable plastics .Elevator or ramp suffice .

Screening for Mycoplasma in new cell lines and biopsies . Planning of tissue culture labs Renovations – Choose location carefully Access – Doorways are wide and high to allow for installations Quarantine - .Containment of biopsies and cell lines posing biohazard .

washup. preparation. Layout of aseptic room or suite Six main functions – sterile handling. sterilization and storage Location for sterile handling should be at one end of room .Preparation.Wash up and sterilization facilities at other end . incubation.In-between . Storage and Incubation .

1 Sterile handling area Sterile area restricted No traffic or disturbance Absence of laminar hoods – use separate room or cubicle .Plastic laminate topped bench .Freestanding or sealed to wall . 4.3.

Legroom with space for pumps.3. Cart or trolley or folding flap (300-500mm) . Chairs with adjustable seat and height .2 Laminar Hood Less expensive than sterile rooms Hoods – Operator’s arms enter Laminar flow wall or ceiling units – Operator is part of work area . aspirators . Wear sterile caps and gowns . 4.

refrigerator.3.3 Quarantine and Containment Separate room with door or lock Separate incubators. freezer. centrifuge.4. supplies and disposal Newly imported cell lines or biopsies Act as Level II containment – biohazard cabinet or pathogen trap .

4.4 Service bench Close to sterile handling area Drawer units below and open shelves above Store sterile glasswares. pipettes. plastics. screw caps.3. syringes etc .

4 Incubation Incubators Hot room Less expensive Less expensive than two Lose more heat and incubators slow to recover Insulated to prevent cold spots Avoid wooden furnishings Stainless steel bench or plastic laminate . 4.

run continuously Air circulation – Fan positioned on opposite side of room of fan heater Thermostats – Two separate heaters with their thermostats Overheating – increase of room temperature. Heat generation in hot room Heater – Fan heater provides heat. heat produced from apparatus etc .

Accommodate balances.5. pH meter.5 Preparation area 4. 4.Preparation and sterilization in hoods 4.2 Wash up and sterilization facilities outside tissue culture lab .5. osmometer etc . sorting pipettes.Space for drying glassware.1 Area for media preparation - . packaging and sealing packs for sterilization .

5 Preparation area 4. 4.5 Wash up Raised edge – to avoid spillage Four taps Deionized water provision .

3 Storage Sterile liquids – 25.5. 4. stoppers Filters Gloves and disposal bags . 4 or -20 or – 70 degree Sterile and non sterile glassware Sterile disposable plastics Screw caps.5 Preparation area 4.

5.3 Storage Liquid Nitrogen – Dewars (25-50 L) Large storage vessel (100-150 L) on trolley Storage tanks (500 – 1000 L) Safety – Ventilation + oxygen alarm + replace air in room Co2 storage units –tethered to wall or bench in a rack . 4.

This project is funded by a grant awarded under the President’s Community Based Job Training Grant as implemented by the U. color. Department of Labor’s Employment and Training Administration (CB-15-162-06-60). and against any beneficiary of programs financially assisted under Title I of the Workforce Investment Act of 1998 (WIA). national origin. political affiliation or belief. religion.S. NCC is an equal opportunity employer and does not discriminate on the following basis: against any individual in the United States. . sex. age disability. on the basis of the beneficiary’s citizenship/status as a lawfully admitted immigrant authorized to work in the United States. on the basis of race. or his or her participation in any WIA Title I-financially assisted program or activity.

Department of Labor. usefulness. All other uses require the prior authorization of the copyright owner. The solution was created by the grantee and does not necessarily reflect the official position of the U. . warranties. including any information on linked sites and including. The Department of Labor makes no guarantees. adequacy. express or implied. accuracy of the information or its completeness. or assurances of any kind. with respect to such information.S. Department of Labor’s Employment and Training Administration.S. but not limited to. This solution is copyrighted by the institution that created it. Internal use by an organization and/or personal use by an individual for non-commercial purposes is permissible. continued availability. or ownership. timeliness. Disclaimer This workforce solution was funded by a grant awarded under the President’s Community-Based Job Training Grants as implemented by the U.