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BL21* pLysS Δ rne131 .

N.1 mM IPTG 28 °C – O. Luria-Bertani Luria-Bertani Luria-Bertani Luria-Bertani Broth Broth Broth Broth . Picking the best condition 0 mM IPTG 0 mM IPTG 0. 1% glucose 37 °C – 3h 28 °C – 5h 28°C – O.5 mM IPTG 0.N.

5’ Tetramethylbenzidine (TMB) Positive Control Stop Solution Purified hTG2 1 .3’.5. 16 Kinetic Assay: Procedure Assay Mixture Streptavidin-peroxidase 3.

17 Expected Results and Analysis Functional hTG2 • Spectrophotometry A: 450 nm .This kinetics assay is the basis of our high-throughput screening 1 .