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INSULIN PRODUCTION

FROM Escherichia coli


ANDI SUARES
ANANG WAHIF FATKHURROCHMAN
MUHAMMAD GAUSSIAN
AUFA NAUFALIA NABILA
ALFRISTA RURI NARESWARI
MUTIARA NUR ISNAINI
UYUN NURUL AINI
INTRODUCTION
UP-STREAM PROCESSING
Media & equipment preparation:
The LB broth is prepared It is
antoclaved and ampicillin and
lactose are added (after the
sterilization to prevent denaturation or
destruction). Inoculation is done by
adding the transformed bacteria into the
media.
Preparation of the bioreactor Parts of
the bioreactors are fixed and checked
such as the calibration of the pH
electrode, pO2 probe, exhaust
condensers and air inlet. The
FERMENTATION

Small Large
scaling scaling
(enrichmen (fermentor)
t liquid
culture in
shake
flask)
Contains Optimal conditions:
ampicillin and - Temperature
lactose as - pH
antibiotic.
Monitoring &
MANUFACTURE CONTROL

Cleanliness

Sterility

Purity
DOWNSTREAM PROCESSING
Isolation of crude products

Purification of crude product

Obtaining of insulin chains

Synthesis of active insulin

PR-HPLC to obtain highly purified insulin


TERIMA KASIH