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PLASMONIC

NANOSENSORS
Kelompok 4

M. Rifqi P. 24141000
Pieter Karunia Deo 2414100068
Kadek Sunantara 2414100098
Aryo Prastyo A. 2414100116
Introduction &

1
Single Nanoparticle
Sensors
Methods to
Enhance The LSPR
Shift 2
Illustration of different strategies to improve the sensitivity of
plasmonic nanosensors. There are several kinds of methods
available to influence the local dielectric properties surrounding
plasmonic nanoparticles that are motivated by factors such as
nanoparticle characteristics, amplification schemes, and plasmon
coupling
Strategies for improving the sensitivity of refractive-
index-based plasmonic nanosensors

-The spectral sensitivity of plasmonic nanoparticles is


highly dependent on their size and geometrical
parameters. Early plasmonic nanosensors were often
composed of spherical or quasi-spherical
nanoparticles. Recently, theoretical simulations and
experimental data have demonstrated that anisotropic
nanostructures exhibit higher refractive index sensitivity
than spherical nanoparticles

practical sensing resolution depends on the absolute


magnitude of the plasmon intensity. Therefore,
plasmonic nanostructures with intense absorption or
scattering cross-sections are favourable in their use as
sensors.
Plasmonic-molecular resonance coupling

the demonstration of plasmonic-molecular resonance


coupling for small-molecule detection, coupling of this
kind has been used for the monitoring of reversible
switching of molecules from non-resonant to resonant
states, so-called molecular plasmonic switching
the factors that affect the sensitivity of
plasmon-coupling-based sensors can be organized into
two categories. The first category is related to
characteristics of the plasmonic nanostructures, e.g.,
the size, shape and composition of the nanoparticles. Three of the most important enhancing factors are
The other category involves external factors discussed: increased aggregate size, minimized
interparticle distance, and scattering signals of
plasmonic coupling.

a. Increased aggregate size: enzyme-guided aggregate


formation
The size of aggregates during the detection process
plays an important role in the intensity of the measured
signals. It has been demonstrated that a measurable
signal can be obtained for even relatively large
interparticle distances, provided the size of aggregates
is sufficiently large.
b. Minimized interparticle distance
In addition to increasing the size of aggregated
nanoparticles, minimizing the interparticle distance can
be an effective approach to enhance the sensitivity of
plasmon-coupling-based sensors. This approach could
be particularly important for the case of a nanoparticle
pair, which is an essential scheme for the construction
of molecular rulers and biosensors. It has been
theoretically proven that plasmonic coupling between
nanostructures is highly sensitive to the interparticle
distance
c. Scattering signals of plasmonic coupling

Scattering-based detection yielded a four-ordersof-


magnitude improvement in the detection sensitivity,
enabling the direct detection of unamplified genomic
DNA strands in zeptomole quantities. Lu et al.
developed a colorimetric sensor for adenosine
detection based on aptazyme-directed assembly of
AuNPs [
Enhancing The
Sensitivity of Plasmonic
Nanosensors 3
Enhancing The Sensitivity of Plasmonic
Nanosensors

Recent developments in this field are categorized by


the different sensing strategies:
Refractive index nanosensors
Colorimetric sensing based on plasmo coupling
Amplified sensitivity based on nanoparticle growth
Enhancing The Sensitivity of Plasmonic Nanosensors
Effect of nanoparticle composition, size, and shape on plasmonic
nanosensors

COMPOSITION

Plasmonic nanosensors are almost exclusively made of gold


or silver, LPSR sensing application of other metal, e.g., zinc
oxide, aluminum, and copper have also been investigated.
Enhancing The Sensitivity of Plasmonic Nanosensors
Effect of nanoparticle composition, size, and shape on plasmonic
nanosensors

SIZE

Spectral sensitivity of plasmonic nanoparticles is highly


dependent on their size and geometrical parameters.
Recently, theoritical simulations and experimental data have
demonstrated thar anisotropic nanostructures exhibit higher
refractive index sentivity than spherical nanoparticles.
Enhancing The Sensitivity of Plasmonic Nanosensors
Effect of nanoparticle composition, size, and shape on plasmonic
nanosensors

SHAPE
Enhancing The Sensitivity of Plasmonic Nanosensors
Practical
Implementations 4
Practical Implementation
Sensor berbasis LSPR dengan desain sangat peka terhadap perubahan karakteristik
nanopartikel, keseragaman dalam ukuran nanopartikel, bentuk dan komposisi sehingga
memiliki tingkat sensitivitas dan kehandalan yang tinggi
Dikembangkan sensor yang stabil terhadap pelarut annealing dan anotasi fotothermal
yaitu dengan cara pretreated dalam etanol, albumin air dan serum sapi
Nanopartikel seragam dapat diproduksi dengan menggunakan berbagai teknik termasuk
litografi nanosfer, litografi berpretreated dalam etanol, albumin air dan serum sapi
Practical Implementation Contd

Substrat AgFON difungsikan sebagai penyusun alumina digunakan dalam


deteksi kuantitatif biomarker antraks, kalsium dipicolinate.
Platform alumina meningkatkan masa simpan sensor SERS (lebih dari satu tahun
pada hitungan terakhir). Lapisan alumina dapat difungsikan pada monolayer
yang dirakit sendiri dengan menggunakan ikatan senyawa kimia karboksilat dan
kimia silan.
Practical Implementation Contd

Sensor LSPR dalam bidang


proteomik masih dalam tahap
pengembangan agar substrat dapat
berinteraksi dengan baik dengan
instrumen throughput tinggi.
Baru-baru ini, sensor biochip LSPR
multi-arrayed dikembangkan oleh
Endo dan rekan kerja.
Biochip ini memberikan deteksi
protein yang cepat dalam volume
sampel mikro.
Practical Implementation Contd

Biochip dibuat dalam tiga tahap:


Pertama, sebuah film emas diendapkan ke substrat kaca, kemudian silika nanospheres dilapisi
ke dalam film emas, dan akhirnya lapisan emas lain diendapkan ke nanospher dan substrat
emas yang mendasarinya.
Untuk mendeteksi protein, asam (4,4'-dithiodibutyric acid) terbentuk pada susunan inti-shell.
Biochip ini berpotensi digunakan dalam berbagai aplikasi termasuk deteksi kanker dan
deteksi mikroorganisme.
Conclusion

1. Tantangan para praktisi yaitu dalam hal peningkatan kualitas maupun


kehandalan biosensor LSPR sehingga dapat mencapai kerja maksimumnya
sebagai alat analisis untuk kuantifikasi kimiawi, karakterisator, deteksi
perubahan konformasi, dan identifikasi molekuler.
2. Pada saat ini, kemajuan di bidang ini terus berlanjut dengan cepat. Sebagai
teknik penginderaan yang baru LSPR dikembangkan untuk meningkatkan
resolusi instrumental dan pergeseran spektral nanopartikel,
3. Adapun saat ini teknik fabrikasi dan nanopartikel sudah sangat maju
menjadi lebih seragam dan stabil, dan karena teknik yang lebih beragam dan
saling melengkapi terintegrasi ini masa depan biosensor plasmon terlihat
sangat menjanjikan.
THANKS
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