Outline

1. 2. 3. 4. Adhesives General principles of staining H&E Mounting

Adhesives
1. 2. 3. 4. 5. 6. 7. Mayer·s egg albumin Dried albumin Gelatin Starch paste Plasma Celloiden Chrome alum adhesive

‡ Processes involved:
² Osmosis, solvation, adsorption, absorption

‡ Chemical properties involved:
² Affinity (pH)

‡ Types of stains:
² Natural dyes ² Synthetic dyes
‡ chromophore!

‡

General Methods/terms:
1. 2. 3. 4. 5. 6. Direct Indirect Progressive Regressive Metachromatic Counterstaining/ differentiation 7. Microanatomical 8. Histochemical 10. Histological 11. Metallic impregnation 12. Vital staining 13. Supravital 14. Intravital

‡

Pre-staining considerations:
1. 2. 3. 4. Precautions & proper technique Type of tissue Component of interest Prior reagent used:
1. 2. 3. 4. Deparaffinization (*sections to water) Collodionization / parcelloiden / celloidin Frozen sections Decalcifying agents

Restaining

Removal of coverslip: Xylene ± 24 hrs OR heating Oxalic acid ± 5 min Repeat washing Xylene ± 30 min

KMnO4 ± 5-10 min

Mounting
‡ Characteristics of a good mountant? ‡ Ringing? ‡ Aqueous media
² ² ² ² ² ² ² ² ² Water (w/ glycerine & jelly) Glycerin Gum arabic (Farrant·s medium) Karo corn syrup Canada balsam DPX Xam Permount Clarite

‡ Resinous media

Mounting

H&E
‡ Nuclei - blue - with some metachromasia ‡ Cytoplasm - various shades of pinkidentifying different tissue components

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